LOC100631542 cDNA ORF clone, Amphimedon queenslandica

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100631542
Entrez Gene ID	100631542
Full Name	methionine synthase-like
Gene Type	protein-coding
Organism	Amphimedon queenslandica

mRNA and Protein(s)

mRNA	Protein	Name
XM_020000653.1	XP_019856212.1	LOW QUALITY PROTEIN: methionine synthase-like

Pathways from BioSystems
Gene Ontology

The following LOC100631542 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100631542 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm23046	XM_020000653.1 Latest version!	Amphimedon queenslandica methionine synthase-like (LOC100631542), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100631542 ( XM_020000653.1 ) cDNA ORF clone, Amphimedon queenslandica -> XP_019856212.1 Amphimedon queenslandica methionine synthase-like (LOC100631542), mRNA.

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CloneID	OAm23046
Clone ID Related Accession (Same CDS sequence)	XM_020000653.1
Accession Version	XM_020000653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-17
Organism	Amphimedon queenslandica
Product	LOW QUALITY PROTEIN: methionine synthase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003546599.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amphimedon queenslandica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTACTG AAGTTCCTCT TCACTTTAGA CCAACATACC CCAAGATAAC ACCTGGTTTG 
GGTGAGACAC TAGCTAATGA GATGTGTAAG AGGATAATGG TACTAGATGG AGCAATGGGT 
ACCATGATAC AAGGACACAA CCTCACTGAA GAACAGTTCA GAGGTGAAGA GTTTAAGGAT 
CATCCCAAGC CGTTAAGAGG AGACAATGAC CTCCTCTGCC TAACACAACC TGACATCATT 
TATAATATAC ATAAACAATA TTTTGAAGCA GGTGCTGATT TTTCTGAGAC AAACACATTC 
AATGGAACAT CAATTTCACA ATCAGATTAT GGACTGGAGC ATTTGGTTTA TTGTATTAAT 
AAATCAGCTG CTGAAATTGC CAGGCAAGCA GCTGCTGACG TCAGTAAAGC AACAGGTCTA 
CCCAAGTATG TTGTTGGAGC ACTGGGTCCA ACCAACAGAA CATTATCAGT ATCACCCTCA 
GTGGAGAGAC CTGATTTTAG GAATATTACT TTTGATGAAC TGGTTGATTC ATATTCAGAG 
CAGGCCCAGG GTCTCCTTGA TGGTGGGGTT GATGTCCTTA TGGTGGAGAC CATATTTGAT 
ACAGCAAACT CTAAGGCAGC CCTTTATGCC GTTGAGACTC TCTTTGAATC AAAGTACAAA 
CCAATACCAG TCTTTGTGTC AGGTACTATA GTTGATAAAA GTGGAAGAAC ACTGTCTGGT 
CAGACGTCTG AGGCCTTTCT GTTCAGTGTG ACACACTCCA AACCACTTTG TATTGGTCTT 
AACTGTGCTT TAGGTGCTAC TGAGATGCGT CCGTTCATTC AAACTGTCAG TAGATCAACT 
GAAGCTTTTG TACTCTGTTA CCCTAATGCT GGTTTGCCCA ATGCTTTGGG TGGATATGAT 
GAAACTCCTG AAGTTATGGC CAGTCATTTG AAATCTTTTG CAGTTGATGG TCTTGTCAAC 
ATTGTCGGTG GTTGCTGTGG GACCACACCT CTTTTTATTA AAGCTATTGC CGAAGCAGTG 
AAAGGGTTAA AACCAAGGGA ACCGCCCACT AATCTTTATC ATGACGCACT CATACTGTCA 
GGGTTGGAAG GGGTTAAAAT CAATTCCACT ACTAACTTCG TTAACATTGG TGAAAGGTGC 
AATGTTGCTG GGTCAAGACG TTTCGCTCGT TTGATAATTG CCGGAAAATT TGAGGAGGCA 
CTCGCCGTTG CTAAGCAACA GGTTGAGATG GGTGCCCAGG TCCTTGACTT GAATATGGAC 
GAGGGTATGC TGGACGGAGT GAAGGCCATG GCAAGATTCT GTAACTACAT ATCAACTGAA 
CCTGACATTG CTAAACTCCC CCTCTGCATT GACTCCAGTA ATTTCTCGGT GGTTCTTGCT 
GGCCTAAAGT GTTCCCAGGG TAAGTGTATC GTGAACTCCA TTAGTCTCAA AGAAGGGGAG 
GAGGACTTCA TTGAGAAGGC AAAGATTATC AAGAAGTTTG GAGGAGCAGC TGTCGTGATG 
GCCTTTGATG AAGTCGGACA AGCTACTACA ACAGAGAACA AAGTCTCCAT CTGTACAAGG 
TCTTACAATA TTCTGGTTGA TGTTGTTGGG TTCAATCCAA ATGACATCAT CTTTGATCCA 
AACATCCTCA CCATAGCAAC GGGAATGGAG GAGCATAACA ACTATGGGAT ATCGTTTATT 
GAGGCAACGA GATTAATTAA ACAAACATTA CCTGGTGCTA GGATCAGTGG TGGCTTGTCT 
AATTTATCGT TTTCATTTCG TGGGAAGGAG GTCATTAGGG AGGCAATGCA CAGTGTCTTT 
CTCTACCATG CAATACAAGC TGGTATGGAC ATGGGTATTG TTAATGCCGG TAACCTCCCA 
CTTTATGATG ACATTAAAAA AGATTTATTG AATTTATGTG AAGACCTGCT CTGGAATAGA 
GACCCAAATA CGACTGAGAA ACTCCTCGAA TATGCTCAGT CGCTGGGTGA AGGTGCTAAG 
AAGCAGGTGG CAACTGATGA ATGGAGGAAT GCCACTGTTG AAGAAAGACT GGAATATTCA 
CTAGTTAAAG GTATTGATAA GTTTGTGGTA TCAGATACTG AAGAGGCTCG TTCTAATTTC 
GATAAGTATC CACGCCCACT TCATATAATT GAAGGGCCAC TGATGTCTGG TATGAGTATT 
GTTGGCGATC TCTTTGGATC AGGAAAAATG TTTCTTCCAC AAGTGATCAA ATCTGCTCGT 
GTCATGAAGA AAGCCGTCGG GTACCTGATT CCTTTTATGG AAGAGGAGAA GGAGAAAGCA 
GCAAAGGAAG AAGGAACTGT CATCTCTGAA GATGAAAACG ATAGATTCTC GGGTACTGTT 
GTTATAGCGA CTGTCAAAGG TGATGTACAT GATATAGGGA AGAATATTGT AGGTGTGGTC 
CTCGGCTGCA ATAACTTCAG AGTTATTGAT TTGGGAGTAA TGACACCTTG TGAGAAGATA 
ATAGAGACAG CAAGAAAAAA TAAAGCAGAT ATTATTGGTC TCTCTGGTTT AATTACTCCG 
TCGTTGGATG AGATGATTTA CGTTGCCAAG GAGATGGAGA GGGAGGGCCT AAAGATACCG 
CTACTGATAG GAGGAGCTAC TACCACAAAG ACTCATACAG CTGTCAAGAT TGCGCCATGC 
TACAGTCAGC CCACTATACA TGTTGTTGCT CTGAATGTGA ACTGCTATAA ACCTGGTAAG 
GATATTAATG GGGACTTTAA TAATTTTAAT ACTATATTGT TCTCTTGTCT CTCCTTTTCA 
GTAAAACCCA GGCAGTTGGG TATTACAGTG TTCCAGGATT ACGACTTGAA CCGTTTGGTA 
TCTTACATTG ACTGGAAACC TTTCTTTGAC GTCTGGCAGC TGAAGGGGAA GTACCCAAAC 
AGAGGGTACC CTAAAATATT CAATGACAAG GACGTGGGTG CTGAAGCTAA GCGTATATAC 
ATTGATGATG CTCAGGTTTT ATTGAATTGT ATCATTAAAG AGAAACTGTT AATAGCTAGA 
GGAGTAGTGG GTATATTTAA AGCTGTGAGA GATGGAGATG ACATCAAGAT ACTGTCAGAA 
GAGAATACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019856212.1
CDS	42..3113
Translation	MPTEVPLHFRPTYPKITPGLGETLANEMCKRIMVLDGAMGTMIQGHNLTEEQFRGEEFKDHPKPLRGDNDLLCLTQPDIIYNIHKQYFEAGADFSETNTFNGTSISQSDYGLEHLVYCINKSAAEIARQAAADVSKATGLPKYVVGALGPTNRTLSVSPSVERPDFRNITFDELVDSYSEQAQGLLDGGVDVLMVETIFDTANSKAALYAVETLFESKYKPIPVFVSGTIVDKSGRTLSGQTSEAFLFSVTHSKPLCIGLNCALGATEMRPFIQTVSRSTEAFVLCYPNAGLPNALGGYDETPEVMASHLKSFAVDGLVNIVGGCCGTTPLFIKAIAEAVKGLKPREPPTNLYHDALILSGLEGVKINSTTNFVNIGERCNVAGSRRFARLIIAGKFEEALAVAKQQVEMGAQVLDLNMDEGMLDGVKAMARFCNYISTEPDIAKLPLCIDSSNFSVVLAGLKCSQGKCIVNSISLKEGEEDFIEKAKIIKKFGGAAVVMAFDEVGQATTTENKVSICTRSYNILVDVVGFNPNDIIFDPNILTIATGMEEHNNYGISFIEATRLIKQTLPGARISGGLSNLSFSFRGKEVIREAMHSVFLYHAIQAGMDMGIVNAGNLPLYDDIKKDLLNLCEDLLWNRDPNTTEKLLEYAQSLGEGAKKQVATDEWRNATVEERLEYSLVKGIDKFVVSDTEEARSNFDKYPRPLHIIEGPLMSGMSIVGDLFGSGKMFLPQVIKSARVMKKAVGYLIPFMEEEKEKAAKEEGTVISEDENDRFSGTVVIATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCEKIIETARKNKADIIGLSGLITPSLDEMIYVAKEMEREGLKIPLLIGGATTTKTHTAVKIAPCYSQPTIHVVALNVNCYKPGKDINGDFNNFNTILFSCLSFSVKPRQLGITVFQDYDLNRLVSYIDWKPFFDVWQLKGKYPNRGYPKIFNDKDVGAEAKRIYIDDAQVLLNCIIKEKLLIARGVVGIFKAVRDGDDIKILSEENT

Target ORF information:

RefSeq Version	XM_020000653.1
Organism	Amphimedon queenslandica
Definition	Amphimedon queenslandica methionine synthase-like (LOC100631542), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020000653.1

ORF Insert Sequence:

ATGCCTACTG AAGTTCCTCT TCACTTTAGA CCAACATACC CCAAGATAAC ACCTGGTTTG 
GGTGAGACAC TAGCTAATGA GATGTGTAAG AGGATAATGG TACTAGATGG AGCAATGGGT 
ACCATGATAC AAGGACACAA CCTCACTGAA GAACAGTTCA GAGGTGAAGA GTTTAAGGAT 
CATCCCAAGC CGTTAAGAGG AGACAATGAC CTCCTCTGCC TAACACAACC TGACATCATT 
TATAATATAC ATAAACAATA TTTTGAAGCA GGTGCTGATT TTTCTGAGAC AAACACATTC 
AATGGAACAT CAATTTCACA ATCAGATTAT GGACTGGAGC ATTTGGTTTA TTGTATTAAT 
AAATCAGCTG CTGAAATTGC CAGGCAAGCA GCTGCTGACG TCAGTAAAGC AACAGGTCTA 
CCCAAGTATG TTGTTGGAGC ACTGGGTCCA ACCAACAGAA CATTATCAGT ATCACCCTCA 
GTGGAGAGAC CTGATTTTAG GAATATTACT TTTGATGAAC TGGTTGATTC ATATTCAGAG 
CAGGCCCAGG GTCTCCTTGA TGGTGGGGTT GATGTCCTTA TGGTGGAGAC CATATTTGAT 
ACAGCAAACT CTAAGGCAGC CCTTTATGCC GTTGAGACTC TCTTTGAATC AAAGTACAAA 
CCAATACCAG TCTTTGTGTC AGGTACTATA GTTGATAAAA GTGGAAGAAC ACTGTCTGGT 
CAGACGTCTG AGGCCTTTCT GTTCAGTGTG ACACACTCCA AACCACTTTG TATTGGTCTT 
AACTGTGCTT TAGGTGCTAC TGAGATGCGT CCGTTCATTC AAACTGTCAG TAGATCAACT 
GAAGCTTTTG TACTCTGTTA CCCTAATGCT GGTTTGCCCA ATGCTTTGGG TGGATATGAT 
GAAACTCCTG AAGTTATGGC CAGTCATTTG AAATCTTTTG CAGTTGATGG TCTTGTCAAC 
ATTGTCGGTG GTTGCTGTGG GACCACACCT CTTTTTATTA AAGCTATTGC CGAAGCAGTG 
AAAGGGTTAA AACCAAGGGA ACCGCCCACT AATCTTTATC ATGACGCACT CATACTGTCA 
GGGTTGGAAG GGGTTAAAAT CAATTCCACT ACTAACTTCG TTAACATTGG TGAAAGGTGC 
AATGTTGCTG GGTCAAGACG TTTCGCTCGT TTGATAATTG CCGGAAAATT TGAGGAGGCA 
CTCGCCGTTG CTAAGCAACA GGTTGAGATG GGTGCCCAGG TCCTTGACTT GAATATGGAC 
GAGGGTATGC TGGACGGAGT GAAGGCCATG GCAAGATTCT GTAACTACAT ATCAACTGAA 
CCTGACATTG CTAAACTCCC CCTCTGCATT GACTCCAGTA ATTTCTCGGT GGTTCTTGCT 
GGCCTAAAGT GTTCCCAGGG TAAGTGTATC GTGAACTCCA TTAGTCTCAA AGAAGGGGAG 
GAGGACTTCA TTGAGAAGGC AAAGATTATC AAGAAGTTTG GAGGAGCAGC TGTCGTGATG 
GCCTTTGATG AAGTCGGACA AGCTACTACA ACAGAGAACA AAGTCTCCAT CTGTACAAGG 
TCTTACAATA TTCTGGTTGA TGTTGTTGGG TTCAATCCAA ATGACATCAT CTTTGATCCA 
AACATCCTCA CCATAGCAAC GGGAATGGAG GAGCATAACA ACTATGGGAT ATCGTTTATT 
GAGGCAACGA GATTAATTAA ACAAACATTA CCTGGTGCTA GGATCAGTGG TGGCTTGTCT 
AATTTATCGT TTTCATTTCG TGGGAAGGAG GTCATTAGGG AGGCAATGCA CAGTGTCTTT 
CTCTACCATG CAATACAAGC TGGTATGGAC ATGGGTATTG TTAATGCCGG TAACCTCCCA 
CTTTATGATG ACATTAAAAA AGATTTATTG AATTTATGTG AAGACCTGCT CTGGAATAGA 
GACCCAAATA CGACTGAGAA ACTCCTCGAA TATGCTCAGT CGCTGGGTGA AGGTGCTAAG 
AAGCAGGTGG CAACTGATGA ATGGAGGAAT GCCACTGTTG AAGAAAGACT GGAATATTCA 
CTAGTTAAAG GTATTGATAA GTTTGTGGTA TCAGATACTG AAGAGGCTCG TTCTAATTTC 
GATAAGTATC CACGCCCACT TCATATAATT GAAGGGCCAC TGATGTCTGG TATGAGTATT 
GTTGGCGATC TCTTTGGATC AGGAAAAATG TTTCTTCCAC AAGTGATCAA ATCTGCTCGT 
GTCATGAAGA AAGCCGTCGG GTACCTGATT CCTTTTATGG AAGAGGAGAA GGAGAAAGCA 
GCAAAGGAAG AAGGAACTGT CATCTCTGAA GATGAAAACG ATAGATTCTC GGGTACTGTT 
GTTATAGCGA CTGTCAAAGG TGATGTACAT GATATAGGGA AGAATATTGT AGGTGTGGTC 
CTCGGCTGCA ATAACTTCAG AGTTATTGAT TTGGGAGTAA TGACACCTTG TGAGAAGATA 
ATAGAGACAG CAAGAAAAAA TAAAGCAGAT ATTATTGGTC TCTCTGGTTT AATTACTCCG 
TCGTTGGATG AGATGATTTA CGTTGCCAAG GAGATGGAGA GGGAGGGCCT AAAGATACCG 
CTACTGATAG GAGGAGCTAC TACCACAAAG ACTCATACAG CTGTCAAGAT TGCGCCATGC 
TACAGTCAGC CCACTATACA TGTTGTTGCT CTGAATGTGA ACTGCTATAA ACCTGGTAAG 
GATATTAATG GGGACTTTAA TAATTTTAAT ACTATATTGT TCTCTTGTCT CTCCTTTTCA 
GTAAAACCCA GGCAGTTGGG TATTACAGTG TTCCAGGATT ACGACTTGAA CCGTTTGGTA 
TCTTACATTG ACTGGAAACC TTTCTTTGAC GTCTGGCAGC TGAAGGGGAA GTACCCAAAC 
AGAGGGTACC CTAAAATATT CAATGACAAG GACGTGGGTG CTGAAGCTAA GCGTATATAC 
ATTGATGATG CTCAGGTTTT ATTGAATTGT ATCATTAAAG AGAAACTGTT AATAGCTAGA 
GGAGTAGTGG GTATATTTAA AGCTGTGAGA GATGGAGATG ACATCAAGAT ACTGTCAGAA 
GAGAATACAT AA