LOC108744097 cDNA ORF clone, Agrilus planipennis(emerald ash borer)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108744097
Entrez Gene ID	108744097
Full Name	ankyrin repeat domain-containing protein 27-like
Gene Type	protein-coding
Organism	Agrilus planipennis(emerald ash borer)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018479691.1	XP_018335193.1	ankyrin repeat domain-containing protein 27-like
XM_018479691.2	XP_018335193.1	ankyrin repeat domain-containing protein 27-like

Pathways from BioSystems
Gene Ontology

The following LOC108744097 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108744097 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAg14348	XM_018479691.2 Latest version!	Agrilus planipennis ankyrin repeat domain-containing protein 27-like (LOC108744097), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OAg14348	XM_018479691.1	Agrilus planipennis ankyrin repeat domain-containing protein 27-like (LOC108744097), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108744097 ( XM_018479691.1 ) cDNA ORF clone, Agrilus planipennis(emerald ash borer) -> XP_018335193.1 Agrilus planipennis ankyrin repeat domain-containing protein 27-like (LOC108744097), mRNA.

Price & Availability↑

CloneID	OAg14348
Clone ID Related Accession (Same CDS sequence)	XM_018479691.1 , XM_018479691.2
Accession Version	XM_018479691.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-21
Organism	Agrilus planipennis(emerald ash borer)
Product	ankyrin repeat domain-containing protein 27-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017259606.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Agrilus planipennis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGG AGTATGATGA GTCAATGACA GACAATCCAT TTTTTCAAGA AATTCAGAAG 
AACTACCAAA ACCTTATCCA CGAAGCAATT AAAGAAAAAT GGATATTCTG CATACCGCGA 
AGAGGAACAA TTTATGGTCA CAATTTGAAT GCGAACGACA TTTTTGAACA TGTTCTAGTG 
CCATATCCTA AAACTAAAAA TAATTGCTAC AGAACGTTAA CTAATAAAAA CGTGCACATT 
AAAAATAAGT GTCTTCATAT TAACAGAAGT CCCTTAGAAA GCAAGAACTT TAAAATATTA 
TTTGAGGAGA CTTTTTATGT AAGTAACCAA GAAAAATATA CTGTATGGTG CATCAATAAA 
CCTTTACATT ATTTGCACAA TGCAGAAATC CACTTTGATA GTAATAAAAT TAATACTTTA 
CAAGATTGCA TCAATCTTTT AAGTAAATAT TGTGTTGTTA CTGATATTTT AAAGTTAATA 
GAAAAGGAAT GTAACAAAAT ATTAGCATTA CATAAACATT TGGAAACTGA AAGTCTACAG 
AATTTACGTA ACATTACCAA AGAATTGTTT ATAAATGTTT TAGATAAAGT TATGAAATTT 
GTAAATGTAA ATGAGATGGA AACATCAACT ACTCTTGACA ACTTCAAAAT ATCTGTTGAG 
ACATACATAC AATTTATTAT ATACAAAAGA CACTTCCCAG CAGTTTGCAA TGCTACGGCT 
TATTTGGACA GTAGTCTTAA TAAAATTATA AGGAACAGTT TTCATGTAGA GTGTAAGGAT 
TTAAATATAG ATTCATCACT ACACGACTAC ATATCTTCTG CTAAATTGCA ATTAAATAAA 
ATCAACAATT TTCAGACAAT TTTAGACAAA GTTAATTGCT TAAAGTCTTC TTTCCAGTTT 
TGTAAACTCA AAAATATTAC AAATAACACA AAACTTCTTT CTGCTGATGA CTTTATAAAT 
ATAGCTATAT TTATAATTAT TAAATCAAAT GTTTACAACT GGGTGGGAAA TGTGACATAC 
ATTACTCAAT TTATTTGTAA TAATTTCTAC TCCTTTAATG AAAACTCCTT TTTTATTACT 
ACTCTTGAGG CTGCAGTGCA ACACATACAG AACAGAGGAA TACTAACTTT AAAGAATGAA 
ATTGTTAAAA ACGACTTAAT TAATAATTTT AGTACTAAAT TAGAGTTGCT GTATGAGCAC 
ATTAAAAGTA ATAACTTTAT GAAATTTCAA GAACTGTTTG CTACTATTAC TCATAAACCT 
ATAGTTCAGT ACAATCAACA ACTTTGCCAT CCTTTATGTT CCTGTGAATC TTGTGAGACT 
GTATTAAAAG ATGAAATTAA TATTGATACT GTTGACGAAA AAGGATGTAC ATTGCTTCAC 
ATGGCTTCTT ATTATGGAAG ATCGCAAATA GTAGACGAAC TCATCCATAA AGGAGCCAAT 
GTAAACTGTG TAGATTTTAT GAACAATTCT CCATTGCATT ATGCAAGCAG TAGAGGAAAC 
CAAGATATAC TTTTGCTTCT TTTACATGCA AGGAGTGAAG TGAATTTAAA AGATTATAAT 
GGAAATACAC CCCTTCATCT AGCTTCTTAC AATGGTCATG AAAATTGTGT GAAAGCCTTA 
CTTTATAGTA CAATACAGAA AGAAAAAGCT GTAGATATAA ATTGTCAGAA TAATTCTGGA 
GACACAGCTT TACATTTGGC TAGCAAATTG GGTTTTGTAG AGATTGTCGG CATTCTTCTT 
CAGTATGGAA GTAATCCAAA CATAAAAAAC CATAAAAATA TAGACGCAGT CGAACTGGCT 
CACAACTATT ACGTAAAGCA AATGATAATT TTAACTAAAC TACACGGAGT AGAAAATACA 
GATAAACACA CAAGCAATAA TAACGTTACT AAACCTAATA ACAACGATCA AACTGATAAA 
AATATGGAAA ATCCTGTATT AAATGATATT CATACAAAAG GTTACAAGCA AATGAAAAAG 
GTTGAGTTAG TGTTGAAGGC TATAGAAAAC AACGATACTC CATTGATGTG TTACCACCTG 
GGAATACCAT CGTCCCACAC TAGCGCCTTA CAACAAACAA TTGCAGGAGA ATGTCATCCC 
TTATGCCTAT GCGAAAAATG TCAAACAAAT GGCGAGATAG AACACGTAAA ATCTTTCCCG 
CCTGTTTCTA ACGCTATCCA CGTAAACATT TGCAACACTG GTGGATACAC CCCTCTTCAT 
ATAGCAACAA AATATGGTAG AAGCGAAATG GTACGTCTAC TGCTTGACAG TGGGTCGTCA 
GTTAATGCTA AAACATTCAA ACATCAGTTT ACTCCTTTGC ACATTGCTTG CCTATATCAA 
AGAGTGAATT GCATTCGAGA ATTATTGAAA TCAGGAGTCT GCGATGTAGA TACACAAGAT 
GCTAAAGGTA ACACGCCCCT TCATTATGCA GTTTCCGTAA ATAATGCAAG AATTGTAGAA 
ATTTTGCTAG GGGCTGGGGC AAATGTTACA TTAAAAAACA TATATGATAA GACATCTCAT 
CAAGAAGCCA ATGAAAGAAT GCTACTTAAT ATTTCGAAAT TATTTAAAAA AAGATATGAA 
AAATTGGTCG ACGATTGTGA ATTACCGTTA GAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018335193.1
CDS	117..2735
Translation	MCMEYDESMTDNPFFQEIQKNYQNLIHEAIKEKWIFCIPRRGTIYGHNLNANDIFEHVLVPYPKTKNNCYRTLTNKNVHIKNKCLHINRSPLESKNFKILFEETFYVSNQEKYTVWCINKPLHYLHNAEIHFDSNKINTLQDCINLLSKYCVVTDILKLIEKECNKILALHKHLETESLQNLRNITKELFINVLDKVMKFVNVNEMETSTTLDNFKISVETYIQFIIYKRHFPAVCNATAYLDSSLNKIIRNSFHVECKDLNIDSSLHDYISSAKLQLNKINNFQTILDKVNCLKSSFQFCKLKNITNNTKLLSADDFINIAIFIIIKSNVYNWVGNVTYITQFICNNFYSFNENSFFITTLEAAVQHIQNRGILTLKNEIVKNDLINNFSTKLELLYEHIKSNNFMKFQELFATITHKPIVQYNQQLCHPLCSCESCETVLKDEINIDTVDEKGCTLLHMASYYGRSQIVDELIHKGANVNCVDFMNNSPLHYASSRGNQDILLLLLHARSEVNLKDYNGNTPLHLASYNGHENCVKALLYSTIQKEKAVDINCQNNSGDTALHLASKLGFVEIVGILLQYGSNPNIKNHKNIDAVELAHNYYVKQMIILTKLHGVENTDKHTSNNNVTKPNNNDQTDKNMENPVLNDIHTKGYKQMKKVELVLKAIENNDTPLMCYHLGIPSSHTSALQQTIAGECHPLCLCEKCQTNGEIEHVKSFPPVSNAIHVNICNTGGYTPLHIATKYGRSEMVRLLLDSGSSVNAKTFKHQFTPLHIACLYQRVNCIRELLKSGVCDVDTQDAKGNTPLHYAVSVNNARIVEILLGAGANVTLKNIYDKTSHQEANERMLLNISKLFKKRYEKLVDDCELPLEI

Target ORF information:

RefSeq Version	XM_018479691.1
Organism	Agrilus planipennis(emerald ash borer)
Definition	Agrilus planipennis ankyrin repeat domain-containing protein 27-like (LOC108744097), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018479691.1

ORF Insert Sequence:

ATGTGCATGG AGTATGATGA GTCAATGACA GACAATCCAT TTTTTCAAGA AATTCAGAAG 
AACTACCAAA ACCTTATCCA CGAAGCAATT AAAGAAAAAT GGATATTCTG CATACCGCGA 
AGAGGAACAA TTTATGGTCA CAATTTGAAT GCGAACGACA TTTTTGAACA TGTTCTAGTG 
CCATATCCTA AAACTAAAAA TAATTGCTAC AGAACGTTAA CTAATAAAAA CGTGCACATT 
AAAAATAAGT GTCTTCATAT TAACAGAAGT CCCTTAGAAA GCAAGAACTT TAAAATATTA 
TTTGAGGAGA CTTTTTATGT AAGTAACCAA GAAAAATATA CTGTATGGTG CATCAATAAA 
CCTTTACATT ATTTGCACAA TGCAGAAATC CACTTTGATA GTAATAAAAT TAATACTTTA 
CAAGATTGCA TCAATCTTTT AAGTAAATAT TGTGTTGTTA CTGATATTTT AAAGTTAATA 
GAAAAGGAAT GTAACAAAAT ATTAGCATTA CATAAACATT TGGAAACTGA AAGTCTACAG 
AATTTACGTA ACATTACCAA AGAATTGTTT ATAAATGTTT TAGATAAAGT TATGAAATTT 
GTAAATGTAA ATGAGATGGA AACATCAACT ACTCTTGACA ACTTCAAAAT ATCTGTTGAG 
ACATACATAC AATTTATTAT ATACAAAAGA CACTTCCCAG CAGTTTGCAA TGCTACGGCT 
TATTTGGACA GTAGTCTTAA TAAAATTATA AGGAACAGTT TTCATGTAGA GTGTAAGGAT 
TTAAATATAG ATTCATCACT ACACGACTAC ATATCTTCTG CTAAATTGCA ATTAAATAAA 
ATCAACAATT TTCAGACAAT TTTAGACAAA GTTAATTGCT TAAAGTCTTC TTTCCAGTTT 
TGTAAACTCA AAAATATTAC AAATAACACA AAACTTCTTT CTGCTGATGA CTTTATAAAT 
ATAGCTATAT TTATAATTAT TAAATCAAAT GTTTACAACT GGGTGGGAAA TGTGACATAC 
ATTACTCAAT TTATTTGTAA TAATTTCTAC TCCTTTAATG AAAACTCCTT TTTTATTACT 
ACTCTTGAGG CTGCAGTGCA ACACATACAG AACAGAGGAA TACTAACTTT AAAGAATGAA 
ATTGTTAAAA ACGACTTAAT TAATAATTTT AGTACTAAAT TAGAGTTGCT GTATGAGCAC 
ATTAAAAGTA ATAACTTTAT GAAATTTCAA GAACTGTTTG CTACTATTAC TCATAAACCT 
ATAGTTCAGT ACAATCAACA ACTTTGCCAT CCTTTATGTT CCTGTGAATC TTGTGAGACT 
GTATTAAAAG ATGAAATTAA TATTGATACT GTTGACGAAA AAGGATGTAC ATTGCTTCAC 
ATGGCTTCTT ATTATGGAAG ATCGCAAATA GTAGACGAAC TCATCCATAA AGGAGCCAAT 
GTAAACTGTG TAGATTTTAT GAACAATTCT CCATTGCATT ATGCAAGCAG TAGAGGAAAC 
CAAGATATAC TTTTGCTTCT TTTACATGCA AGGAGTGAAG TGAATTTAAA AGATTATAAT 
GGAAATACAC CCCTTCATCT AGCTTCTTAC AATGGTCATG AAAATTGTGT GAAAGCCTTA 
CTTTATAGTA CAATACAGAA AGAAAAAGCT GTAGATATAA ATTGTCAGAA TAATTCTGGA 
GACACAGCTT TACATTTGGC TAGCAAATTG GGTTTTGTAG AGATTGTCGG CATTCTTCTT 
CAGTATGGAA GTAATCCAAA CATAAAAAAC CATAAAAATA TAGACGCAGT CGAACTGGCT 
CACAACTATT ACGTAAAGCA AATGATAATT TTAACTAAAC TACACGGAGT AGAAAATACA 
GATAAACACA CAAGCAATAA TAACGTTACT AAACCTAATA ACAACGATCA AACTGATAAA 
AATATGGAAA ATCCTGTATT AAATGATATT CATACAAAAG GTTACAAGCA AATGAAAAAG 
GTTGAGTTAG TGTTGAAGGC TATAGAAAAC AACGATACTC CATTGATGTG TTACCACCTG 
GGAATACCAT CGTCCCACAC TAGCGCCTTA CAACAAACAA TTGCAGGAGA ATGTCATCCC 
TTATGCCTAT GCGAAAAATG TCAAACAAAT GGCGAGATAG AACACGTAAA ATCTTTCCCG 
CCTGTTTCTA ACGCTATCCA CGTAAACATT TGCAACACTG GTGGATACAC CCCTCTTCAT 
ATAGCAACAA AATATGGTAG AAGCGAAATG GTACGTCTAC TGCTTGACAG TGGGTCGTCA 
GTTAATGCTA AAACATTCAA ACATCAGTTT ACTCCTTTGC ACATTGCTTG CCTATATCAA 
AGAGTGAATT GCATTCGAGA ATTATTGAAA TCAGGAGTCT GCGATGTAGA TACACAAGAT 
GCTAAAGGTA ACACGCCCCT TCATTATGCA GTTTCCGTAA ATAATGCAAG AATTGTAGAA 
ATTTTGCTAG GGGCTGGGGC AAATGTTACA TTAAAAAACA TATATGATAA GACATCTCAT 
CAAGAAGCCA ATGAAAGAAT GCTACTTAAT ATTTCGAAAT TATTTAAAAA AAGATATGAA 
AAATTGGTCG ACGATTGTGA ATTACCGTTA GAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108744097 ( XM_018479691.2 ) cDNA ORF clone, Agrilus planipennis(emerald ash borer) -> XP_018335193.1 Agrilus planipennis ankyrin repeat domain-containing protein 27-like (LOC108744097), mRNA.

Price & Availability↑

CloneID	OAg14348
Clone ID Related Accession (Same CDS sequence)	XM_018479691.1 , XM_018479691.2
Accession Version	XM_018479691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-29
Organism	Agrilus planipennis(emerald ash borer)
Product	ankyrin repeat domain-containing protein 27-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020438920.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 30, 2018 this sequence version replaced XM_018479691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Agrilus planipennis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGG AGTATGATGA GTCAATGACA GACAATCCAT TTTTTCAAGA AATTCAGAAG 
AACTACCAAA ACCTTATCCA CGAAGCAATT AAAGAAAAAT GGATATTCTG CATACCGCGA 
AGAGGAACAA TTTATGGTCA CAATTTGAAT GCGAACGACA TTTTTGAACA TGTTCTAGTG 
CCATATCCTA AAACTAAAAA TAATTGCTAC AGAACGTTAA CTAATAAAAA CGTGCACATT 
AAAAATAAGT GTCTTCATAT TAACAGAAGT CCCTTAGAAA GCAAGAACTT TAAAATATTA 
TTTGAGGAGA CTTTTTATGT AAGTAACCAA GAAAAATATA CTGTATGGTG CATCAATAAA 
CCTTTACATT ATTTGCACAA TGCAGAAATC CACTTTGATA GTAATAAAAT TAATACTTTA 
CAAGATTGCA TCAATCTTTT AAGTAAATAT TGTGTTGTTA CTGATATTTT AAAGTTAATA 
GAAAAGGAAT GTAACAAAAT ATTAGCATTA CATAAACATT TGGAAACTGA AAGTCTACAG 
AATTTACGTA ACATTACCAA AGAATTGTTT ATAAATGTTT TAGATAAAGT TATGAAATTT 
GTAAATGTAA ATGAGATGGA AACATCAACT ACTCTTGACA ACTTCAAAAT ATCTGTTGAG 
ACATACATAC AATTTATTAT ATACAAAAGA CACTTCCCAG CAGTTTGCAA TGCTACGGCT 
TATTTGGACA GTAGTCTTAA TAAAATTATA AGGAACAGTT TTCATGTAGA GTGTAAGGAT 
TTAAATATAG ATTCATCACT ACACGACTAC ATATCTTCTG CTAAATTGCA ATTAAATAAA 
ATCAACAATT TTCAGACAAT TTTAGACAAA GTTAATTGCT TAAAGTCTTC TTTCCAGTTT 
TGTAAACTCA AAAATATTAC AAATAACACA AAACTTCTTT CTGCTGATGA CTTTATAAAT 
ATAGCTATAT TTATAATTAT TAAATCAAAT GTTTACAACT GGGTGGGAAA TGTGACATAC 
ATTACTCAAT TTATTTGTAA TAATTTCTAC TCCTTTAATG AAAACTCCTT TTTTATTACT 
ACTCTTGAGG CTGCAGTGCA ACACATACAG AACAGAGGAA TACTAACTTT AAAGAATGAA 
ATTGTTAAAA ACGACTTAAT TAATAATTTT AGTACTAAAT TAGAGTTGCT GTATGAGCAC 
ATTAAAAGTA ATAACTTTAT GAAATTTCAA GAACTGTTTG CTACTATTAC TCATAAACCT 
ATAGTTCAGT ACAATCAACA ACTTTGCCAT CCTTTATGTT CCTGTGAATC TTGTGAGACT 
GTATTAAAAG ATGAAATTAA TATTGATACT GTTGACGAAA AAGGATGTAC ATTGCTTCAC 
ATGGCTTCTT ATTATGGAAG ATCGCAAATA GTAGACGAAC TCATCCATAA AGGAGCCAAT 
GTAAACTGTG TAGATTTTAT GAACAATTCT CCATTGCATT ATGCAAGCAG TAGAGGAAAC 
CAAGATATAC TTTTGCTTCT TTTACATGCA AGGAGTGAAG TGAATTTAAA AGATTATAAT 
GGAAATACAC CCCTTCATCT AGCTTCTTAC AATGGTCATG AAAATTGTGT GAAAGCCTTA 
CTTTATAGTA CAATACAGAA AGAAAAAGCT GTAGATATAA ATTGTCAGAA TAATTCTGGA 
GACACAGCTT TACATTTGGC TAGCAAATTG GGTTTTGTAG AGATTGTCGG CATTCTTCTT 
CAGTATGGAA GTAATCCAAA CATAAAAAAC CATAAAAATA TAGACGCAGT CGAACTGGCT 
CACAACTATT ACGTAAAGCA AATGATAATT TTAACTAAAC TACACGGAGT AGAAAATACA 
GATAAACACA CAAGCAATAA TAACGTTACT AAACCTAATA ACAACGATCA AACTGATAAA 
AATATGGAAA ATCCTGTATT AAATGATATT CATACAAAAG GTTACAAGCA AATGAAAAAG 
GTTGAGTTAG TGTTGAAGGC TATAGAAAAC AACGATACTC CATTGATGTG TTACCACCTG 
GGAATACCAT CGTCCCACAC TAGCGCCTTA CAACAAACAA TTGCAGGAGA ATGTCATCCC 
TTATGCCTAT GCGAAAAATG TCAAACAAAT GGCGAGATAG AACACGTAAA ATCTTTCCCG 
CCTGTTTCTA ACGCTATCCA CGTAAACATT TGCAACACTG GTGGATACAC CCCTCTTCAT 
ATAGCAACAA AATATGGTAG AAGCGAAATG GTACGTCTAC TGCTTGACAG TGGGTCGTCA 
GTTAATGCTA AAACATTCAA ACATCAGTTT ACTCCTTTGC ACATTGCTTG CCTATATCAA 
AGAGTGAATT GCATTCGAGA ATTATTGAAA TCAGGAGTCT GCGATGTAGA TACACAAGAT 
GCTAAAGGTA ACACGCCCCT TCATTATGCA GTTTCCGTAA ATAATGCAAG AATTGTAGAA 
ATTTTGCTAG GGGCTGGGGC AAATGTTACA TTAAAAAACA TATATGATAA GACATCTCAT 
CAAGAAGCCA ATGAAAGAAT GCTACTTAAT ATTTCGAAAT TATTTAAAAA AAGATATGAA 
AAATTGGTCG ACGATTGTGA ATTACCGTTA GAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018335193.1
CDS	242..2860
Translation	MCMEYDESMTDNPFFQEIQKNYQNLIHEAIKEKWIFCIPRRGTIYGHNLNANDIFEHVLVPYPKTKNNCYRTLTNKNVHIKNKCLHINRSPLESKNFKILFEETFYVSNQEKYTVWCINKPLHYLHNAEIHFDSNKINTLQDCINLLSKYCVVTDILKLIEKECNKILALHKHLETESLQNLRNITKELFINVLDKVMKFVNVNEMETSTTLDNFKISVETYIQFIIYKRHFPAVCNATAYLDSSLNKIIRNSFHVECKDLNIDSSLHDYISSAKLQLNKINNFQTILDKVNCLKSSFQFCKLKNITNNTKLLSADDFINIAIFIIIKSNVYNWVGNVTYITQFICNNFYSFNENSFFITTLEAAVQHIQNRGILTLKNEIVKNDLINNFSTKLELLYEHIKSNNFMKFQELFATITHKPIVQYNQQLCHPLCSCESCETVLKDEINIDTVDEKGCTLLHMASYYGRSQIVDELIHKGANVNCVDFMNNSPLHYASSRGNQDILLLLLHARSEVNLKDYNGNTPLHLASYNGHENCVKALLYSTIQKEKAVDINCQNNSGDTALHLASKLGFVEIVGILLQYGSNPNIKNHKNIDAVELAHNYYVKQMIILTKLHGVENTDKHTSNNNVTKPNNNDQTDKNMENPVLNDIHTKGYKQMKKVELVLKAIENNDTPLMCYHLGIPSSHTSALQQTIAGECHPLCLCEKCQTNGEIEHVKSFPPVSNAIHVNICNTGGYTPLHIATKYGRSEMVRLLLDSGSSVNAKTFKHQFTPLHIACLYQRVNCIRELLKSGVCDVDTQDAKGNTPLHYAVSVNNARIVEILLGAGANVTLKNIYDKTSHQEANERMLLNISKLFKKRYEKLVDDCELPLEI

Target ORF information:

RefSeq Version	XM_018479691.2
Organism	Agrilus planipennis(emerald ash borer)
Definition	Agrilus planipennis ankyrin repeat domain-containing protein 27-like (LOC108744097), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018479691.2

ORF Insert Sequence:

ATGTGCATGG AGTATGATGA GTCAATGACA GACAATCCAT TTTTTCAAGA AATTCAGAAG 
AACTACCAAA ACCTTATCCA CGAAGCAATT AAAGAAAAAT GGATATTCTG CATACCGCGA 
AGAGGAACAA TTTATGGTCA CAATTTGAAT GCGAACGACA TTTTTGAACA TGTTCTAGTG 
CCATATCCTA AAACTAAAAA TAATTGCTAC AGAACGTTAA CTAATAAAAA CGTGCACATT 
AAAAATAAGT GTCTTCATAT TAACAGAAGT CCCTTAGAAA GCAAGAACTT TAAAATATTA 
TTTGAGGAGA CTTTTTATGT AAGTAACCAA GAAAAATATA CTGTATGGTG CATCAATAAA 
CCTTTACATT ATTTGCACAA TGCAGAAATC CACTTTGATA GTAATAAAAT TAATACTTTA 
CAAGATTGCA TCAATCTTTT AAGTAAATAT TGTGTTGTTA CTGATATTTT AAAGTTAATA 
GAAAAGGAAT GTAACAAAAT ATTAGCATTA CATAAACATT TGGAAACTGA AAGTCTACAG 
AATTTACGTA ACATTACCAA AGAATTGTTT ATAAATGTTT TAGATAAAGT TATGAAATTT 
GTAAATGTAA ATGAGATGGA AACATCAACT ACTCTTGACA ACTTCAAAAT ATCTGTTGAG 
ACATACATAC AATTTATTAT ATACAAAAGA CACTTCCCAG CAGTTTGCAA TGCTACGGCT 
TATTTGGACA GTAGTCTTAA TAAAATTATA AGGAACAGTT TTCATGTAGA GTGTAAGGAT 
TTAAATATAG ATTCATCACT ACACGACTAC ATATCTTCTG CTAAATTGCA ATTAAATAAA 
ATCAACAATT TTCAGACAAT TTTAGACAAA GTTAATTGCT TAAAGTCTTC TTTCCAGTTT 
TGTAAACTCA AAAATATTAC AAATAACACA AAACTTCTTT CTGCTGATGA CTTTATAAAT 
ATAGCTATAT TTATAATTAT TAAATCAAAT GTTTACAACT GGGTGGGAAA TGTGACATAC 
ATTACTCAAT TTATTTGTAA TAATTTCTAC TCCTTTAATG AAAACTCCTT TTTTATTACT 
ACTCTTGAGG CTGCAGTGCA ACACATACAG AACAGAGGAA TACTAACTTT AAAGAATGAA 
ATTGTTAAAA ACGACTTAAT TAATAATTTT AGTACTAAAT TAGAGTTGCT GTATGAGCAC 
ATTAAAAGTA ATAACTTTAT GAAATTTCAA GAACTGTTTG CTACTATTAC TCATAAACCT 
ATAGTTCAGT ACAATCAACA ACTTTGCCAT CCTTTATGTT CCTGTGAATC TTGTGAGACT 
GTATTAAAAG ATGAAATTAA TATTGATACT GTTGACGAAA AAGGATGTAC ATTGCTTCAC 
ATGGCTTCTT ATTATGGAAG ATCGCAAATA GTAGACGAAC TCATCCATAA AGGAGCCAAT 
GTAAACTGTG TAGATTTTAT GAACAATTCT CCATTGCATT ATGCAAGCAG TAGAGGAAAC 
CAAGATATAC TTTTGCTTCT TTTACATGCA AGGAGTGAAG TGAATTTAAA AGATTATAAT 
GGAAATACAC CCCTTCATCT AGCTTCTTAC AATGGTCATG AAAATTGTGT GAAAGCCTTA 
CTTTATAGTA CAATACAGAA AGAAAAAGCT GTAGATATAA ATTGTCAGAA TAATTCTGGA 
GACACAGCTT TACATTTGGC TAGCAAATTG GGTTTTGTAG AGATTGTCGG CATTCTTCTT 
CAGTATGGAA GTAATCCAAA CATAAAAAAC CATAAAAATA TAGACGCAGT CGAACTGGCT 
CACAACTATT ACGTAAAGCA AATGATAATT TTAACTAAAC TACACGGAGT AGAAAATACA 
GATAAACACA CAAGCAATAA TAACGTTACT AAACCTAATA ACAACGATCA AACTGATAAA 
AATATGGAAA ATCCTGTATT AAATGATATT CATACAAAAG GTTACAAGCA AATGAAAAAG 
GTTGAGTTAG TGTTGAAGGC TATAGAAAAC AACGATACTC CATTGATGTG TTACCACCTG 
GGAATACCAT CGTCCCACAC TAGCGCCTTA CAACAAACAA TTGCAGGAGA ATGTCATCCC 
TTATGCCTAT GCGAAAAATG TCAAACAAAT GGCGAGATAG AACACGTAAA ATCTTTCCCG 
CCTGTTTCTA ACGCTATCCA CGTAAACATT TGCAACACTG GTGGATACAC CCCTCTTCAT 
ATAGCAACAA AATATGGTAG AAGCGAAATG GTACGTCTAC TGCTTGACAG TGGGTCGTCA 
GTTAATGCTA AAACATTCAA ACATCAGTTT ACTCCTTTGC ACATTGCTTG CCTATATCAA 
AGAGTGAATT GCATTCGAGA ATTATTGAAA TCAGGAGTCT GCGATGTAGA TACACAAGAT 
GCTAAAGGTA ACACGCCCCT TCATTATGCA GTTTCCGTAA ATAATGCAAG AATTGTAGAA 
ATTTTGCTAG GGGCTGGGGC AAATGTTACA TTAAAAAACA TATATGATAA GACATCTCAT 
CAAGAAGCCA ATGAAAGAAT GCTACTTAAT ATTTCGAAAT TATTTAAAAA AAGATATGAA 
AAATTGGTCG ACGATTGTGA ATTACCGTTA GAAATTTAA