LOC108736187 cDNA ORF clone, Agrilus planipennis(emerald ash borer)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108736187
Entrez Gene ID	108736187
Full Name	eye-specific diacylglycerol kinase
Gene Type	protein-coding
Organism	Agrilus planipennis(emerald ash borer)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025981393.1	XP_025837178.1	eye-specific diacylglycerol kinase
XM_018468516.1	XP_018324018.1	eye-specific diacylglycerol kinase

Pathways from BioSystems
Gene Ontology

The following LOC108736187 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108736187 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAg19965	XM_018468516.1 Latest version!	Agrilus planipennis eye-specific diacylglycerol kinase (LOC108736187), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAg61107	XM_025981393.1 Latest version!	Agrilus planipennis eye-specific diacylglycerol kinase (LOC108736187), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108736187 ( XM_018468516.1 ) cDNA ORF clone, Agrilus planipennis(emerald ash borer) -> XP_018324018.1 Agrilus planipennis eye-specific diacylglycerol kinase (LOC108736187), mRNA.

Price & Availability↑

CloneID	OAg19965
Clone ID Related Accession (Same CDS sequence)	XM_018468516.1
Accession Version	XM_018468516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-21
Organism	Agrilus planipennis(emerald ash borer)
Product	eye-specific diacylglycerol kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017259697.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Agrilus planipennis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TTCGCACGAC GTTTCAGCGA TCGCGCACCC CTACCGGCAA TGAAATGAAG 
ACACAAAGCA GCCTCGAAGT ACCCAAACAG ATCCGATCTG CTTCATTCGA TGAGATCCAA 
GCTAAACGTC TGCAAACCAA CCCGCAATCC TCGGACCCAA ATTCATTTCT TCGGGTGCCT 
CAGTTTCAGA ATACGCGTTC CAAAAGCGTC GATTCGAACG CGAGCGATGA ATCGGGCAAT 
TTCCTGGAAG TTCCGTCGAA AAAGTTTCCA CGACGAAAGT CATCCGGTGC GCGACCGTTC 
GTATGCGTTC ACTGTATGTA CCTGGAGGAA TCGAAACAAC ACGCGGCAGA AAAACCACCC 
CCTAAACCTC CAGATTTCCG ATCGAGTCTC AGTATCAGCG AATTCTCCTC ATCATCTTCC 
GAAGAAGACA TGGCTCCAAT TCCTCATTGT TCTATAACGG TCACCGTATC GCCAACGCCT 
AACTCGCCTC CACCAAGACC TCCTCCGCCT ATTCTAACCT CTCCTGTCAT TGAATATTAT 
TCGCCCGAAA GCGAGCCAAT TCCTCCAGCA ACAGCTGTCA GGAGACGATC AATACAAAGG 
CAAGAGGCAT TTTTGGGAGA ATCGACTGGT AATTCACTGG AGGATATGAA AGATGTTGCA 
TCTGAAATGA CGTCTGACGG AACATCGGAG GGAGCATTGT CGGAAGGTGA TTGCATAGCG 
ATTCCGGACG TGGTGGTTAG AGATATTTAC CTCCAGGTAC CGGACTTAAA AAGAGACCGA 
GCAGCATCGG TGGACTCGTG CTTTTCTAAG GTGTCTCAGG GTAAGACGGA GGAATTGCAA 
CATTCTGGTG TTAGCTTGGA GGTTCCAGTG GGGCCGAATG TCGCGTTACG GTCCAGATCC 
GTTGATATCG TTCTACCCAC GGACGAACAG GCGAGGTATA AAGCCCTTGC AATGGCTGCC 
CCTCCAGACG AAACCAGTTC GGATAGAAGA GGAAAACCGA GTACCGAGTC AGAGCGATCT 
CTCCGGAACA CTCCAGATTG GACGGAAGGA GCTATTGATG GTGATCATTT GTGGGTCCCA 
ACATCCGTAT CTGGAGACTT CTGCTACGTG GGCGACCCTG AGTGCACGAA GCACGGAATC 
CGTTTGAAGT GTTCCGCTTG CAATATTGTT GCCCATCAAA AATGCATTCC CATATTGCTG 
GAACGAATAC GTTTCACTTG CAAGCCCACC TTCAGAGATG TCGGTTTAAG ACAATATCGA 
GAGCAATCTA ACGTGCAGCA CCACTGGGTC CATCGGCGGT CAGAGAAAGG GAAATGTAAA 
CAATGCGGAA AATCATTCCA ATCGAAATTA TCATTCGGCG CTAAAGAAAT CGTTGCAATA 
AATTGCTCTT GGTGCAAAGT GGCTTTCCAC AACAAAGAAA CTTGTTTCGG GTCCCATCGT 
ATGGAAGAAG CATGTAATTT AGGTGTACAT AGCAGTATTA TAGTTCCTCC TTCGTGGATT 
ATCAAGTTAC CTGGCAAAGG GAACTTTAAA TCCAGCCTTA GGAAATCTTC GAAGAAAAAT 
TCGTCGAGCA AACGACGGAA TAAAGAAAAG GATAAGGACC ATAAAGCATT TGTAATCAAG 
CCTATACCAT CAAAAACAGT TACGCCTGTG ATAGTATTTA TAAATCCCAA ATCAGGGGGC 
AATCAAGGTA CTAAACTACT GCAGAAGTTC CAATGGCTAC TCAATCCCAG ACAGGTGTTT 
GACCTAACAC AAGGTGGACC CAAGATGGGT TTGGAACTGT TCAAGAAGGT GCCGAACCTT 
CGGGTTTTGG CCTGTGGTGG TGACGGTACT GTTGGATGGG TCTTAAGTGT CATCGACCAA 
ATCGGCATAT CTCCAGCTCC AGCGGTTGGA GTTCTTCCAT TAGGCACTGG CAACGATTTG 
GCACGAGCAT TAGGATGGGG AGGGGGATAC ACTGATGAAC CAATTTCAAA AATTCTTATG 
AACATAAGCA ATAGCGAAAC TGTGTTATTG GACAGGTGGT CGTTGGAAGT GGAAAAAAAT 
CCGGACGCCG ACCCTAACGA AGGCGGAAAG GATAATCTAC CGTTGAACGT TGTAAATAAT 
TATTTTTCAC TCGGTGTCGA TGCTCATATA GCTTTAGAAT TTCACGAAGC CAGAGAGGCT 
CATCCTGAAA AATTTAATTC AAGACTGAGA AATAAAATGT TTTACGGGCA AATGGGAGGT 
AAGGACCTTC TCAAGCGAAA ATGGAAGGGT CTCGCCGAAT TAGTTACCTT AGAATGTGAC 
GGACGTGATC TTACGCCAAA GCTGAAAGAA CTCAAAGTGC ATGCGATTGT ATTTCTCAAC 
ATTCCAAGTT ATGGTGGTGG TACCAGGCCC TGGAACAGGG CAATGGGATC ATCGGAACCA 
AGCACTGACG ACGGTCTAAT CGAAGTTGTG GGTCTGACGA CCTATCAGCT GCCTCTTCTC 
CAAGCAGGTG GTCACGGTAC ATGCATCACT CAATGCAAGA ACGCGAGGAT CGTCACTTCG 
AAGACGATTC CAATGCAAGT TGATGGAGAA GCGTGTAAAC TGGCCCCATC GATAATTAAT 
CTAACGTTGC TCAACAAGGC CACCATGCTG GCGAAACGAA AAGGAGGCGG AAAACACCAT 
GTACCACGTG TACCACTCGA AGATCTTACC ATCCAGGTGC ACAGAATCGC GATGAGGGAC 
TATGAACAGC ATCACTACGA CAGGGATATG CTAACGCAGT CAGCCATCAA GGTCGGCACG 
ATAGAAGTAA ATCCTATGTC CGATTTGGAA CAGGTCAGAG CCCAAATCGA CCAGTATATG 
GAAGAATGTA ATCAAACGAA ACCTGCCGAA TGGTGTTTTC TCGATTCGTG TACAGCAGAG 
CGTTTTTTTC GTATCGACAA AGCTCAAGAG CATCTACACT ACGTTTCGGA CATAGCGTAC 
GAATCTCTTT ACATCCTCGA TTGCGGTAGC GTCACAGTAC CTCAAACTCC CGACGACGAT 
GCTACCGTTC CCTCAGCCAG TTCAATGCAC ACCACCTACA TGGTACCTGA AATGAAAGTT 
AGTTGCAGTA GTGAGGGTAG TCTGGATTCG CCCAGCGATC ATCCCCCAAT TTCGCCGAAA 
ACCGCCCTCT CACCCGACGA CGACGTACAG CAACCCATTC CGGTCGATAA ACAAAACGGG 
GAAAGGCGCC TGAACATTTA CAACGCTATT AAAATTAAAA GCGATGTAAT CGAAAAGCCC 
AACGAAGCCC TTATAAAAGC TGCTATTGTG GGAGATTTGC AAACGATAAA AAAGGTACAT 
TCCATGGGAC ATTCCTTAAC GACAACGGAT TCCTCGGGGC AGACCATCTT ACATCACGCT 
GTGAGAAATA GTCACAAAGA AATTGTGAAA TACGTTTTGA GCACAGCTCC AACTTCAATT 
TTAGAAGCTA CCGACAATGC GGGCCAGACC GCTCTACATA AAGCCGCTTC TTACAAATGT 
CGCAACATCT GTTGTATGCT AATCGCGGCT GGAGCTTCTC TGGAAGTGAA AGACCAAAAC 
GGGCTCACCC CCAGGCTTCT GGCCATCCAA GCTAACGATC ATGATCTAGG GGCGTACCTC 
GAAAGTCAAG AACACTTCAG AATGATATCC AATGATCTAG GCTCTGAGGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018324018.1
CDS	473..4126
Translation	MQRLRTTFQRSRTPTGNEMKTQSSLEVPKQIRSASFDEIQAKRLQTNPQSSDPNSFLRVPQFQNTRSKSVDSNASDESGNFLEVPSKKFPRRKSSGARPFVCVHCMYLEESKQHAAEKPPPKPPDFRSSLSISEFSSSSSEEDMAPIPHCSITVTVSPTPNSPPPRPPPPILTSPVIEYYSPESEPIPPATAVRRRSIQRQEAFLGESTGNSLEDMKDVASEMTSDGTSEGALSEGDCIAIPDVVVRDIYLQVPDLKRDRAASVDSCFSKVSQGKTEELQHSGVSLEVPVGPNVALRSRSVDIVLPTDEQARYKALAMAAPPDETSSDRRGKPSTESERSLRNTPDWTEGAIDGDHLWVPTSVSGDFCYVGDPECTKHGIRLKCSACNIVAHQKCIPILLERIRFTCKPTFRDVGLRQYREQSNVQHHWVHRRSEKGKCKQCGKSFQSKLSFGAKEIVAINCSWCKVAFHNKETCFGSHRMEEACNLGVHSSIIVPPSWIIKLPGKGNFKSSLRKSSKKNSSSKRRNKEKDKDHKAFVIKPIPSKTVTPVIVFINPKSGGNQGTKLLQKFQWLLNPRQVFDLTQGGPKMGLELFKKVPNLRVLACGGDGTVGWVLSVIDQIGISPAPAVGVLPLGTGNDLARALGWGGGYTDEPISKILMNISNSETVLLDRWSLEVEKNPDADPNEGGKDNLPLNVVNNYFSLGVDAHIALEFHEAREAHPEKFNSRLRNKMFYGQMGGKDLLKRKWKGLAELVTLECDGRDLTPKLKELKVHAIVFLNIPSYGGGTRPWNRAMGSSEPSTDDGLIEVVGLTTYQLPLLQAGGHGTCITQCKNARIVTSKTIPMQVDGEACKLAPSIINLTLLNKATMLAKRKGGGKHHVPRVPLEDLTIQVHRIAMRDYEQHHYDRDMLTQSAIKVGTIEVNPMSDLEQVRAQIDQYMEECNQTKPAEWCFLDSCTAERFFRIDKAQEHLHYVSDIAYESLYILDCGSVTVPQTPDDDATVPSASSMHTTYMVPEMKVSCSSEGSLDSPSDHPPISPKTALSPDDDVQQPIPVDKQNGERRLNIYNAIKIKSDVIEKPNEALIKAAIVGDLQTIKKVHSMGHSLTTTDSSGQTILHHAVRNSHKEIVKYVLSTAPTSILEATDNAGQTALHKAASYKCRNICCMLIAAGASLEVKDQNGLTPRLLAIQANDHDLGAYLESQEHFRMISNDLGSEV

Target ORF information:

RefSeq Version	XM_018468516.1
Organism	Agrilus planipennis(emerald ash borer)
Definition	Agrilus planipennis eye-specific diacylglycerol kinase (LOC108736187), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018468516.1

ORF Insert Sequence:

ATGCAAAGGC TTCGCACGAC GTTTCAGCGA TCGCGCACCC CTACCGGCAA TGAAATGAAG 
ACACAAAGCA GCCTCGAAGT ACCCAAACAG ATCCGATCTG CTTCATTCGA TGAGATCCAA 
GCTAAACGTC TGCAAACCAA CCCGCAATCC TCGGACCCAA ATTCATTTCT TCGGGTGCCT 
CAGTTTCAGA ATACGCGTTC CAAAAGCGTC GATTCGAACG CGAGCGATGA ATCGGGCAAT 
TTCCTGGAAG TTCCGTCGAA AAAGTTTCCA CGACGAAAGT CATCCGGTGC GCGACCGTTC 
GTATGCGTTC ACTGTATGTA CCTGGAGGAA TCGAAACAAC ACGCGGCAGA AAAACCACCC 
CCTAAACCTC CAGATTTCCG ATCGAGTCTC AGTATCAGCG AATTCTCCTC ATCATCTTCC 
GAAGAAGACA TGGCTCCAAT TCCTCATTGT TCTATAACGG TCACCGTATC GCCAACGCCT 
AACTCGCCTC CACCAAGACC TCCTCCGCCT ATTCTAACCT CTCCTGTCAT TGAATATTAT 
TCGCCCGAAA GCGAGCCAAT TCCTCCAGCA ACAGCTGTCA GGAGACGATC AATACAAAGG 
CAAGAGGCAT TTTTGGGAGA ATCGACTGGT AATTCACTGG AGGATATGAA AGATGTTGCA 
TCTGAAATGA CGTCTGACGG AACATCGGAG GGAGCATTGT CGGAAGGTGA TTGCATAGCG 
ATTCCGGACG TGGTGGTTAG AGATATTTAC CTCCAGGTAC CGGACTTAAA AAGAGACCGA 
GCAGCATCGG TGGACTCGTG CTTTTCTAAG GTGTCTCAGG GTAAGACGGA GGAATTGCAA 
CATTCTGGTG TTAGCTTGGA GGTTCCAGTG GGGCCGAATG TCGCGTTACG GTCCAGATCC 
GTTGATATCG TTCTACCCAC GGACGAACAG GCGAGGTATA AAGCCCTTGC AATGGCTGCC 
CCTCCAGACG AAACCAGTTC GGATAGAAGA GGAAAACCGA GTACCGAGTC AGAGCGATCT 
CTCCGGAACA CTCCAGATTG GACGGAAGGA GCTATTGATG GTGATCATTT GTGGGTCCCA 
ACATCCGTAT CTGGAGACTT CTGCTACGTG GGCGACCCTG AGTGCACGAA GCACGGAATC 
CGTTTGAAGT GTTCCGCTTG CAATATTGTT GCCCATCAAA AATGCATTCC CATATTGCTG 
GAACGAATAC GTTTCACTTG CAAGCCCACC TTCAGAGATG TCGGTTTAAG ACAATATCGA 
GAGCAATCTA ACGTGCAGCA CCACTGGGTC CATCGGCGGT CAGAGAAAGG GAAATGTAAA 
CAATGCGGAA AATCATTCCA ATCGAAATTA TCATTCGGCG CTAAAGAAAT CGTTGCAATA 
AATTGCTCTT GGTGCAAAGT GGCTTTCCAC AACAAAGAAA CTTGTTTCGG GTCCCATCGT 
ATGGAAGAAG CATGTAATTT AGGTGTACAT AGCAGTATTA TAGTTCCTCC TTCGTGGATT 
ATCAAGTTAC CTGGCAAAGG GAACTTTAAA TCCAGCCTTA GGAAATCTTC GAAGAAAAAT 
TCGTCGAGCA AACGACGGAA TAAAGAAAAG GATAAGGACC ATAAAGCATT TGTAATCAAG 
CCTATACCAT CAAAAACAGT TACGCCTGTG ATAGTATTTA TAAATCCCAA ATCAGGGGGC 
AATCAAGGTA CTAAACTACT GCAGAAGTTC CAATGGCTAC TCAATCCCAG ACAGGTGTTT 
GACCTAACAC AAGGTGGACC CAAGATGGGT TTGGAACTGT TCAAGAAGGT GCCGAACCTT 
CGGGTTTTGG CCTGTGGTGG TGACGGTACT GTTGGATGGG TCTTAAGTGT CATCGACCAA 
ATCGGCATAT CTCCAGCTCC AGCGGTTGGA GTTCTTCCAT TAGGCACTGG CAACGATTTG 
GCACGAGCAT TAGGATGGGG AGGGGGATAC ACTGATGAAC CAATTTCAAA AATTCTTATG 
AACATAAGCA ATAGCGAAAC TGTGTTATTG GACAGGTGGT CGTTGGAAGT GGAAAAAAAT 
CCGGACGCCG ACCCTAACGA AGGCGGAAAG GATAATCTAC CGTTGAACGT TGTAAATAAT 
TATTTTTCAC TCGGTGTCGA TGCTCATATA GCTTTAGAAT TTCACGAAGC CAGAGAGGCT 
CATCCTGAAA AATTTAATTC AAGACTGAGA AATAAAATGT TTTACGGGCA AATGGGAGGT 
AAGGACCTTC TCAAGCGAAA ATGGAAGGGT CTCGCCGAAT TAGTTACCTT AGAATGTGAC 
GGACGTGATC TTACGCCAAA GCTGAAAGAA CTCAAAGTGC ATGCGATTGT ATTTCTCAAC 
ATTCCAAGTT ATGGTGGTGG TACCAGGCCC TGGAACAGGG CAATGGGATC ATCGGAACCA 
AGCACTGACG ACGGTCTAAT CGAAGTTGTG GGTCTGACGA CCTATCAGCT GCCTCTTCTC 
CAAGCAGGTG GTCACGGTAC ATGCATCACT CAATGCAAGA ACGCGAGGAT CGTCACTTCG 
AAGACGATTC CAATGCAAGT TGATGGAGAA GCGTGTAAAC TGGCCCCATC GATAATTAAT 
CTAACGTTGC TCAACAAGGC CACCATGCTG GCGAAACGAA AAGGAGGCGG AAAACACCAT 
GTACCACGTG TACCACTCGA AGATCTTACC ATCCAGGTGC ACAGAATCGC GATGAGGGAC 
TATGAACAGC ATCACTACGA CAGGGATATG CTAACGCAGT CAGCCATCAA GGTCGGCACG 
ATAGAAGTAA ATCCTATGTC CGATTTGGAA CAGGTCAGAG CCCAAATCGA CCAGTATATG 
GAAGAATGTA ATCAAACGAA ACCTGCCGAA TGGTGTTTTC TCGATTCGTG TACAGCAGAG 
CGTTTTTTTC GTATCGACAA AGCTCAAGAG CATCTACACT ACGTTTCGGA CATAGCGTAC 
GAATCTCTTT ACATCCTCGA TTGCGGTAGC GTCACAGTAC CTCAAACTCC CGACGACGAT 
GCTACCGTTC CCTCAGCCAG TTCAATGCAC ACCACCTACA TGGTACCTGA AATGAAAGTT 
AGTTGCAGTA GTGAGGGTAG TCTGGATTCG CCCAGCGATC ATCCCCCAAT TTCGCCGAAA 
ACCGCCCTCT CACCCGACGA CGACGTACAG CAACCCATTC CGGTCGATAA ACAAAACGGG 
GAAAGGCGCC TGAACATTTA CAACGCTATT AAAATTAAAA GCGATGTAAT CGAAAAGCCC 
AACGAAGCCC TTATAAAAGC TGCTATTGTG GGAGATTTGC AAACGATAAA AAAGGTACAT 
TCCATGGGAC ATTCCTTAAC GACAACGGAT TCCTCGGGGC AGACCATCTT ACATCACGCT 
GTGAGAAATA GTCACAAAGA AATTGTGAAA TACGTTTTGA GCACAGCTCC AACTTCAATT 
TTAGAAGCTA CCGACAATGC GGGCCAGACC GCTCTACATA AAGCCGCTTC TTACAAATGT 
CGCAACATCT GTTGTATGCT AATCGCGGCT GGAGCTTCTC TGGAAGTGAA AGACCAAAAC 
GGGCTCACCC CCAGGCTTCT GGCCATCCAA GCTAACGATC ATGATCTAGG GGCGTACCTC 
GAAAGTCAAG AACACTTCAG AATGATATCC AATGATCTAG GCTCTGAGGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108736187 ( XM_025981393.1 ) cDNA ORF clone, Agrilus planipennis(emerald ash borer) -> XP_025837178.1 Agrilus planipennis eye-specific diacylglycerol kinase (LOC108736187), mRNA.

Price & Availability↑

CloneID	OAg61107
Clone ID Related Accession (Same CDS sequence)	XM_025981393.1
Accession Version	XM_025981393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-29
Organism	Agrilus planipennis(emerald ash borer)
Product	eye-specific diacylglycerol kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020442469.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Agrilus planipennis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TTCGCACGAC GTTTCAGCGA TCGCGCACCC CTACCGGCAA TGAAATGAAG 
ACACAAAGCA GCCTCGAAGT ACCCAAACAG ATCCGATCTG CTTCATTCGA TGAGATCCAA 
GCTAAACGTC TGCAAACCAA CCCGCAATCC TCGGACCCAA ATTCATTTCT TCGGGTGCCT 
CAGTTTCAGA ATACGCGTTC CAAAAGCGTC GATTCGAACG CGAGCGATGA ATCGGGCAAT 
TTCCTGGAAG TTCCGTCGAA AAAGTTTCCA CGACGAAAGT CATCCGGTGC GCGACCGTTC 
GTATGCGTTC ACTGTATGTA CCTGGAGGAA TCGAAACAAC ACGCGGCAGA AAAACCACCC 
CCTAAACCTC CAGATTTCCG ATCGAGTCTC AGTATCAGCG AATTCTCCTC ATCATCTTCC 
GAAGAAGACA TGGCTCCAAT TCCTCATTGT TCTATAACGG TCACCGTATC GCCAACGCCT 
AACTCGCCTC CACCAAGACC TCCTCCGCCT ATTCTAACCT CTCCTGTCAT TGAATATTAT 
TCGCCCGAAA GCGAGCCAAT TCCTCCAGCA ACAGCTGTCA GGAGACGATC AATACAAAGG 
CAAGAGGCAT TTTTGGGAGA ATCGACTGGT AATTCACTGG AGGATATGAA AGATGTTGCA 
TCTGAAATGA CGTCTGACGG AACATCGGAG GGAGCATTGT CGGAAGGTGA TTGCATAGCG 
ATTCCGGACG TGGTGGTTAG AGATATTTAC CTCCAGGTAC CGGACTTAAA AAGAGACCGA 
GCAGCATCGG TGGACTCGTG CTTTTCTAAG GTGTCTCAGG GTAAGACGGA GGAATTGCAA 
CATTCTGGTG TTAGCTTGGA GGTTCCAGTG GGGCCGAATG TCGCGTTACG GTCCAGATCC 
GTTGATATCG TTCTACCCAC GGACGAACAG GCGAGGTATA AAGCCCTTGC AATGGCTGCC 
CCTCCAGACG AAACCAGTTC GGATAGAAGA GGAAAACCGA GTACCGAGTC AGAGCGATCT 
CTCCGGAACA CTCCAGATTG GACGGAAGGA GCTATTGATG GTGATCATTT GTGGGTCCCA 
ACATCCGTAT CTGGAGACTT CTGCTACGTG GGCGACCCTG AGTGCACGAA GCACGGAATC 
CGTTTGAAGT GTTCCGCTTG CAATATTGTT GCCCATCAAA AATGCATTCC CATATTGCTG 
GAACGAATAC GTTTCACTTG CAAGCCCACC TTCAGAGATG TCGGTTTAAG ACAATATCGA 
GAGCAATCTA ACGTGCAGCA CCACTGGGTC CATCGGCGGT CAGAGAAAGG GAAATGTAAA 
CAATGCGGAA AATCATTCCA ATCGAAATTA TCATTCGGCG CTAAAGAAAT CGTTGCAATA 
AATTGCTCTT GGTGCAAAGT GGCTTTCCAC AACAAAGAAA CTTGTTTCGG GTCCCATCGT 
ATGGAAGAAG CATGTAATTT AGGTGTACAT AGCAGTATTA TAGTTCCTCC TTCGTGGATT 
ATCAAGTTAC CTGGCAAAGG GAACTTTAAA TCCAGCCTTA GGAAATCTTC GAAGAAAAAT 
TCGTCGAGCA AACGACGGAA TAAAGAAAAG GATAAGGACC ATAAAGCATT TGTAATCAAG 
CCTATACCAT CAAAAACAGT TACGCCTGTG ATAGTATTTA TAAATCCCAA ATCAGGGGGC 
AATCAAGGTA CTAAACTACT GCAGAAGTTC CAATGGCTAC TCAATCCCAG ACAGGTGTTT 
GACCTAACAC AAGGTGGACC CAAGATGGGT TTGGAACTGT TCAAGAAGGT GCCGAACCTT 
CGGGTTTTGG CCTGTGGTGG TGACGGTACT GTTGGATGGG TCTTAAGTGT CATCGACCAA 
ATCGGCATAT CTCCAGCTCC AGCGGTTGGA GTTCTTCCAT TAGGCACTGG CAACGATTTG 
GCACGAGCAT TAGGATGGGG AGGGGGATAC ACTGATGAAC CAATTTCAAA AATTCTTATG 
AACATAAGCA ATAGCGAAAC TGTGTTATTG GACAGGTGGT CGTTGGAAGT GGAAAAAAAT 
CCGGACGCCG ACCCTAACGA AGGCGGAAAG GATAATCTAC CGTTGAACGT TGTAAATAAT 
TATTTTTCAC TCGGTGTCGA TGCTCATATA GCTTTAGAAT TTCACGAAGC CAGAGAGGCT 
CATCCTGAAA AATTTAATTC AAGACTGAGA AATAAAATGT TTTACGGGCA AATGGGAGGT 
AAGGACCTTC TCAAGCGAAA ATGGAAGGGT CTCGCCGAAT TAGTTACCTT AGAATGTGAC 
GGACGTGATC TTACGCCAAA GCTGAAAGAA CTCAAAGTGC ATGCGATTGT ATTTCTCAAC 
ATTCCAAGTT ATGGTGGTGG TACCAGGCCC TGGAACAGGG CAATGGGATC ATCGGAACCA 
AGCACTGACG ACGGTCTAAT CGAAGTTGTG GGTCTGACGA CCTATCAGCT GCCTCTTCTC 
CAAGCAGGTG GTCACGGTAC ATGCATCACT CAATGCAAGA ACGCGAGGAT CGTCACTTCG 
AAGACGATTC CAATGCAAGT TGATGGAGAA GCGTGTAAAC TGGCCCCATC GATAATTAAT 
CTAACGTTGC TCAACAAGGC CACCATGCTG GCGAAACGAA AAGGAGGCGG AAAACACCAT 
GTACCACGTG TACCACTCGA AGATCTTACC ATCCAGGTGC ACAGAATCGC GATGAGGGAC 
TATGAACAGC ATCACTACGA CAGGGATATG CTAACGCAGT CAGCCATCAA GGTCGGCACG 
ATAGAAGTAA ATCCTATGTC CGATTTGGAA CAGGTCAGAG CCCAAATCGA CCAGTATATG 
GAAGAATGTA ATCAAACGAA ACCTGCCGAA TGGTGTTTTC TCGATTCGTG TACAGCAGAG 
CGTTTTTTTC GTATCGACAA AGCTCAAGAG CATCTACACT ACGTTTCGGA CATAGCGTAC 
GAATCTCTTT ACATCCTCGA TTGCGGTAGC GTCACAGTAC CTCAAACTCC CGACGACGAT 
GCTACCGTTC CCTCAGCCAG TTCAATGCAC ACCACCTACA TGGTACCTGA AATGAAAGTT 
AGTAGTGAGG GTAGTCTGGA TTCGCCCAGC GATCATCCCC CAATTTCGCC GAAAACCGCC 
CTCTCACCCG ACGACGACGT ACAGCAACCC ATTCCGGTCG ATAAACAAAA CGGGGAAAGG 
CGCCTGAACA TTTACAACGC TATTAAAATT AAAAGCGATG TAATCGAAAA GCCCAACGAA 
GCCCTTATAA AAGCTGCTAT TGTGGGAGAT TTGCAAACGA TAAAAAAGGT ACATTCCATG 
GGACATTCCT TAACGACAAC GGATTCCTCG GGGCAGACCA TCTTACATCA CGCTGTGAGA 
AATAGTCACA AAGAAATTGT GAAATACGTT TTGAGCACAG CTCCAACTTC AATTTTAGAA 
GCTACCGACA ATGCGGGCCA GACCGCTCTA CATAAAGCCG CTTCTTACAA ATGTCGCAAC 
ATCTGTTGTA TGCTAATCGC GGCTGGAGCT TCTCTGGAAG TGAAAGACCA AAACGGGCTC 
ACCCCCAGGC TTCTGGCCAT CCAAGCTAAC GATCATGATC TAGGGGCGTA CCTCGAAAGT 
CAAGAACACT TCAGAATGAT ATCCAATGAT CTAGGCTCTG AGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025837178.1
CDS	473..4120
Translation	MQRLRTTFQRSRTPTGNEMKTQSSLEVPKQIRSASFDEIQAKRLQTNPQSSDPNSFLRVPQFQNTRSKSVDSNASDESGNFLEVPSKKFPRRKSSGARPFVCVHCMYLEESKQHAAEKPPPKPPDFRSSLSISEFSSSSSEEDMAPIPHCSITVTVSPTPNSPPPRPPPPILTSPVIEYYSPESEPIPPATAVRRRSIQRQEAFLGESTGNSLEDMKDVASEMTSDGTSEGALSEGDCIAIPDVVVRDIYLQVPDLKRDRAASVDSCFSKVSQGKTEELQHSGVSLEVPVGPNVALRSRSVDIVLPTDEQARYKALAMAAPPDETSSDRRGKPSTESERSLRNTPDWTEGAIDGDHLWVPTSVSGDFCYVGDPECTKHGIRLKCSACNIVAHQKCIPILLERIRFTCKPTFRDVGLRQYREQSNVQHHWVHRRSEKGKCKQCGKSFQSKLSFGAKEIVAINCSWCKVAFHNKETCFGSHRMEEACNLGVHSSIIVPPSWIIKLPGKGNFKSSLRKSSKKNSSSKRRNKEKDKDHKAFVIKPIPSKTVTPVIVFINPKSGGNQGTKLLQKFQWLLNPRQVFDLTQGGPKMGLELFKKVPNLRVLACGGDGTVGWVLSVIDQIGISPAPAVGVLPLGTGNDLARALGWGGGYTDEPISKILMNISNSETVLLDRWSLEVEKNPDADPNEGGKDNLPLNVVNNYFSLGVDAHIALEFHEAREAHPEKFNSRLRNKMFYGQMGGKDLLKRKWKGLAELVTLECDGRDLTPKLKELKVHAIVFLNIPSYGGGTRPWNRAMGSSEPSTDDGLIEVVGLTTYQLPLLQAGGHGTCITQCKNARIVTSKTIPMQVDGEACKLAPSIINLTLLNKATMLAKRKGGGKHHVPRVPLEDLTIQVHRIAMRDYEQHHYDRDMLTQSAIKVGTIEVNPMSDLEQVRAQIDQYMEECNQTKPAEWCFLDSCTAERFFRIDKAQEHLHYVSDIAYESLYILDCGSVTVPQTPDDDATVPSASSMHTTYMVPEMKVSSEGSLDSPSDHPPISPKTALSPDDDVQQPIPVDKQNGERRLNIYNAIKIKSDVIEKPNEALIKAAIVGDLQTIKKVHSMGHSLTTTDSSGQTILHHAVRNSHKEIVKYVLSTAPTSILEATDNAGQTALHKAASYKCRNICCMLIAAGASLEVKDQNGLTPRLLAIQANDHDLGAYLESQEHFRMISNDLGSEV

Target ORF information:

RefSeq Version	XM_025981393.1
Organism	Agrilus planipennis(emerald ash borer)
Definition	Agrilus planipennis eye-specific diacylglycerol kinase (LOC108736187), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025981393.1

ORF Insert Sequence:

ATGCAAAGGC TTCGCACGAC GTTTCAGCGA TCGCGCACCC CTACCGGCAA TGAAATGAAG 
ACACAAAGCA GCCTCGAAGT ACCCAAACAG ATCCGATCTG CTTCATTCGA TGAGATCCAA 
GCTAAACGTC TGCAAACCAA CCCGCAATCC TCGGACCCAA ATTCATTTCT TCGGGTGCCT 
CAGTTTCAGA ATACGCGTTC CAAAAGCGTC GATTCGAACG CGAGCGATGA ATCGGGCAAT 
TTCCTGGAAG TTCCGTCGAA AAAGTTTCCA CGACGAAAGT CATCCGGTGC GCGACCGTTC 
GTATGCGTTC ACTGTATGTA CCTGGAGGAA TCGAAACAAC ACGCGGCAGA AAAACCACCC 
CCTAAACCTC CAGATTTCCG ATCGAGTCTC AGTATCAGCG AATTCTCCTC ATCATCTTCC 
GAAGAAGACA TGGCTCCAAT TCCTCATTGT TCTATAACGG TCACCGTATC GCCAACGCCT 
AACTCGCCTC CACCAAGACC TCCTCCGCCT ATTCTAACCT CTCCTGTCAT TGAATATTAT 
TCGCCCGAAA GCGAGCCAAT TCCTCCAGCA ACAGCTGTCA GGAGACGATC AATACAAAGG 
CAAGAGGCAT TTTTGGGAGA ATCGACTGGT AATTCACTGG AGGATATGAA AGATGTTGCA 
TCTGAAATGA CGTCTGACGG AACATCGGAG GGAGCATTGT CGGAAGGTGA TTGCATAGCG 
ATTCCGGACG TGGTGGTTAG AGATATTTAC CTCCAGGTAC CGGACTTAAA AAGAGACCGA 
GCAGCATCGG TGGACTCGTG CTTTTCTAAG GTGTCTCAGG GTAAGACGGA GGAATTGCAA 
CATTCTGGTG TTAGCTTGGA GGTTCCAGTG GGGCCGAATG TCGCGTTACG GTCCAGATCC 
GTTGATATCG TTCTACCCAC GGACGAACAG GCGAGGTATA AAGCCCTTGC AATGGCTGCC 
CCTCCAGACG AAACCAGTTC GGATAGAAGA GGAAAACCGA GTACCGAGTC AGAGCGATCT 
CTCCGGAACA CTCCAGATTG GACGGAAGGA GCTATTGATG GTGATCATTT GTGGGTCCCA 
ACATCCGTAT CTGGAGACTT CTGCTACGTG GGCGACCCTG AGTGCACGAA GCACGGAATC 
CGTTTGAAGT GTTCCGCTTG CAATATTGTT GCCCATCAAA AATGCATTCC CATATTGCTG 
GAACGAATAC GTTTCACTTG CAAGCCCACC TTCAGAGATG TCGGTTTAAG ACAATATCGA 
GAGCAATCTA ACGTGCAGCA CCACTGGGTC CATCGGCGGT CAGAGAAAGG GAAATGTAAA 
CAATGCGGAA AATCATTCCA ATCGAAATTA TCATTCGGCG CTAAAGAAAT CGTTGCAATA 
AATTGCTCTT GGTGCAAAGT GGCTTTCCAC AACAAAGAAA CTTGTTTCGG GTCCCATCGT 
ATGGAAGAAG CATGTAATTT AGGTGTACAT AGCAGTATTA TAGTTCCTCC TTCGTGGATT 
ATCAAGTTAC CTGGCAAAGG GAACTTTAAA TCCAGCCTTA GGAAATCTTC GAAGAAAAAT 
TCGTCGAGCA AACGACGGAA TAAAGAAAAG GATAAGGACC ATAAAGCATT TGTAATCAAG 
CCTATACCAT CAAAAACAGT TACGCCTGTG ATAGTATTTA TAAATCCCAA ATCAGGGGGC 
AATCAAGGTA CTAAACTACT GCAGAAGTTC CAATGGCTAC TCAATCCCAG ACAGGTGTTT 
GACCTAACAC AAGGTGGACC CAAGATGGGT TTGGAACTGT TCAAGAAGGT GCCGAACCTT 
CGGGTTTTGG CCTGTGGTGG TGACGGTACT GTTGGATGGG TCTTAAGTGT CATCGACCAA 
ATCGGCATAT CTCCAGCTCC AGCGGTTGGA GTTCTTCCAT TAGGCACTGG CAACGATTTG 
GCACGAGCAT TAGGATGGGG AGGGGGATAC ACTGATGAAC CAATTTCAAA AATTCTTATG 
AACATAAGCA ATAGCGAAAC TGTGTTATTG GACAGGTGGT CGTTGGAAGT GGAAAAAAAT 
CCGGACGCCG ACCCTAACGA AGGCGGAAAG GATAATCTAC CGTTGAACGT TGTAAATAAT 
TATTTTTCAC TCGGTGTCGA TGCTCATATA GCTTTAGAAT TTCACGAAGC CAGAGAGGCT 
CATCCTGAAA AATTTAATTC AAGACTGAGA AATAAAATGT TTTACGGGCA AATGGGAGGT 
AAGGACCTTC TCAAGCGAAA ATGGAAGGGT CTCGCCGAAT TAGTTACCTT AGAATGTGAC 
GGACGTGATC TTACGCCAAA GCTGAAAGAA CTCAAAGTGC ATGCGATTGT ATTTCTCAAC 
ATTCCAAGTT ATGGTGGTGG TACCAGGCCC TGGAACAGGG CAATGGGATC ATCGGAACCA 
AGCACTGACG ACGGTCTAAT CGAAGTTGTG GGTCTGACGA CCTATCAGCT GCCTCTTCTC 
CAAGCAGGTG GTCACGGTAC ATGCATCACT CAATGCAAGA ACGCGAGGAT CGTCACTTCG 
AAGACGATTC CAATGCAAGT TGATGGAGAA GCGTGTAAAC TGGCCCCATC GATAATTAAT 
CTAACGTTGC TCAACAAGGC CACCATGCTG GCGAAACGAA AAGGAGGCGG AAAACACCAT 
GTACCACGTG TACCACTCGA AGATCTTACC ATCCAGGTGC ACAGAATCGC GATGAGGGAC 
TATGAACAGC ATCACTACGA CAGGGATATG CTAACGCAGT CAGCCATCAA GGTCGGCACG 
ATAGAAGTAA ATCCTATGTC CGATTTGGAA CAGGTCAGAG CCCAAATCGA CCAGTATATG 
GAAGAATGTA ATCAAACGAA ACCTGCCGAA TGGTGTTTTC TCGATTCGTG TACAGCAGAG 
CGTTTTTTTC GTATCGACAA AGCTCAAGAG CATCTACACT ACGTTTCGGA CATAGCGTAC 
GAATCTCTTT ACATCCTCGA TTGCGGTAGC GTCACAGTAC CTCAAACTCC CGACGACGAT 
GCTACCGTTC CCTCAGCCAG TTCAATGCAC ACCACCTACA TGGTACCTGA AATGAAAGTT 
AGTAGTGAGG GTAGTCTGGA TTCGCCCAGC GATCATCCCC CAATTTCGCC GAAAACCGCC 
CTCTCACCCG ACGACGACGT ACAGCAACCC ATTCCGGTCG ATAAACAAAA CGGGGAAAGG 
CGCCTGAACA TTTACAACGC TATTAAAATT AAAAGCGATG TAATCGAAAA GCCCAACGAA 
GCCCTTATAA AAGCTGCTAT TGTGGGAGAT TTGCAAACGA TAAAAAAGGT ACATTCCATG 
GGACATTCCT TAACGACAAC GGATTCCTCG GGGCAGACCA TCTTACATCA CGCTGTGAGA 
AATAGTCACA AAGAAATTGT GAAATACGTT TTGAGCACAG CTCCAACTTC AATTTTAGAA 
GCTACCGACA ATGCGGGCCA GACCGCTCTA CATAAAGCCG CTTCTTACAA ATGTCGCAAC 
ATCTGTTGTA TGCTAATCGC GGCTGGAGCT TCTCTGGAAG TGAAAGACCA AAACGGGCTC 
ACCCCCAGGC TTCTGGCCAT CCAAGCTAAC GATCATGATC TAGGGGCGTA CCTCGAAAGT 
CAAGAACACT TCAGAATGAT ATCCAATGAT CTAGGCTCTG AGGTTTGA