TRPA1 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRPA1
Entrez Gene ID	106968383
Full Name	transient receptor potential cation channel subfamily A member 1
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015064743.1	XP_014920229.1	transient receptor potential cation channel subfamily A member 1
XM_027064393.1	XP_026920194.1	transient receptor potential cation channel subfamily A member 1

Pathways from BioSystems
Gene Ontology

The following TRPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc223524	XM_027064393.1 Latest version!	Acinonyx jubatus transient receptor potential cation channel subfamily A member 1 (TRPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAc40129	XM_015064743.1 Latest version!	Acinonyx jubatus transient receptor potential cation channel, subfamily A, member 1 (TRPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPA1 ( XM_027064393.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026920194.1 Acinonyx jubatus transient receptor potential cation channel subfamily A member 1 (TRPA1), mRNA.

Price & Availability↑

CloneID	OAc223524
Clone ID Related Accession (Same CDS sequence)	XM_027064393.1
Accession Version	XM_027064393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	transient receptor potential cation channel subfamily A member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834742.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGCA GCCTGAGGAA GATGCTGAGC CCTGGGGGTA AGCAGGAGCC CCAGGGCGCA 
GTCTACCAGG GCGTGGAGAA CGGCATGGAC GGCCGCAAGG ACTCGCTGAA GGTGGTTTTT 
GAAGGAAGTA CATGTAGATT TAACTTGATG AAGAAACGAA AGAAGTTAAG CAAATATGAT 
GATGTGAACG CATCCCCTTT GCACCATGCT GCAGAAGAAG GTCAAGTGCA GCTGATGGGG 
ATGATCATCA ATGACTCCTC TTTTGAAGTG CTGAATGTGA TGGATGATTA TGGAAATACC 
CCTCTGCACT GGGCCACAGA AAAAAACCAA GTTGAAAGTG TTAAGTTTCT TCTTAGCAAA 
GGAGCCAACC CAAACCTCCG AAACAGCAAC ATGTTGGCTC CTCTTCACAT CGCTGTGCAG 
GGCTTACACA ATGAGGTGAT GAAGGTCTTG ACTGAGCACA ACAACACTAA TATTAATTTG 
GAAGGAGAAA ATGGAAACAC AGCTGTGATA ATCGCATGCG CCAAAGATAA TAGTGAAGCA 
CTGCAAATCT TGTTAAATAA AGGAGCTAAG CCATGTAAGG CAAATAAATG GGGATGTTTC 
CCTATTCATC AGGCTGCATT CTCAGGTGCC AAAAAATGCA TGGAAATAAT ATTAAAGTTT 
GGTGAGGAAC ATGGGTATAG CAGAGAGTCT CATATTAACT TTGTGAATAA TGCAAAAGCT 
AGTCCCCTCC ACATGGCGGT TCAAAGTGGT GACTTGGAAA TGATTAAAAT GTGCTTGGAC 
AATGGCGCAC AGTTGGATCT GATGGAAAAT GGGAAGTGCA CACCCCTTCA CTTTGCTGCC 
ACCCAGGGAG CCACTGAGAT TGTTAAATTG ATGATATCTT CCTATTCTGG TAGCAGTGAT 
ATCGTCAATG CAGTGGATGG AAATCAGGAG ACACTCCTTC ACAGAGCCTC GTTATTTGAT 
CACCATGAAC TGGCAGACTA CTTAATTTCA GTGGGAGCAG ATATCAATAG CACTGATTCG 
GAAGGACGTT CTCCACTTAT ATTAGCAACT GCTTCTGCAT CTTGGAATAC TGTAAATTTG 
CTACTGTCTA AAGGTGCCCA TGTAGACATA AAAGATCATC TTGGACGTAA TTTTTTGCAT 
TTGACTGTAC AGCAACCTCA TGGATTAAAA AATCTGCGAC CTGAGTTTAT GCAGATGCAA 
CATATTAAAA AGCTGGTAAT GGATGAAGAT GATGACGGGT GTACTCCTCT ACATTATGCA 
TGTAGACACG GGATTCCTGT CTCTGTAGAT AACCTTCTTG ACTTTAATGT GTCCATTCAT 
TCCAAAAGCA AAGATAAGAA ATCACCCTTA CATTTTGCAG CCAGTTATGG GCGTATCAAC 
ACCTGTCAGA GGCTTCTGCA AGATATGAAT GACACGAGGC TTTTGAATGA AGGTGACCTC 
CATGGAATGA CGCCTTTGCA CCTGGCTGCA AAAAATGGGC ATGATAAGGT AGTTCAACTT 
CTTCTGAAAA AAGGTGCATT ATTTCTCAGT GACCACAGTG GCTGGACTGC TTTGCATCAT 
GCTTCCTTGG GCGGGTACAC TCAGACCATG AAGGTCATTC TTGACACTAA CATGAAGTGC 
ACAGATCAGC TAGATGAAGA AGGGAACACT GCACTGCACT TTGCTGCAAG GGAAGGCCAT 
GCCAAAGCCG TCGCACTTCT TTTGAGTTAT AATGCTGACA TCGTCCTGAA CAAGCAGCAG 
GCCTCCTTTT TGCATGTTGC AATTCACAAT AAGAGGAAGG ACGTGGTTCT TACGACCATC 
AAGAACAAAA GATGGGAAGA ATGCCTTAAG GTTTTTAGTC ATTATTCTCC AAGCAATAAA 
TGTCCAGTTA TGGAAATGGT AGAATACCTC CCTGAATGCA TGAAGGTCCT TTTAGATTGT 
TGCATGATGC CCTCCAAAAG AGACAAGTCC TGCCAAGACT ATTATATTGA GTATAATTTC 
AGGTATCTTC AGTGTCCATT AGAATTTACC AAGAAAGTAA CACCTGCACA GGATGTTACA 
TATGAGCCTC TTACAACCCT CAACGCAATG GTACAACATA ACCGCATAGA GCTTCTCAAT 
CATCCTGTAT GTAAAGAATA TTTACTTATG AAATGGTTGG CTTATGGGTT TAGAGCCCAT 
ATGATGAACC TAGGATCTTA CTGTCTTGGT CTCCTGCCTA TGACCTTTCT TGTTGTCAGT 
ATAAAGCCAG GTATGGCTTT CAACTCAACT GGAATCATCA ATGAAACTAG TGACCATTCA 
GAAATATTAG ACACCAAGAA TTCCTATTCT ATAAAAGTTT GTATGATTTT AGTTTTTTTA 
TCAAGTATAT TTGGATACTG CAAAGAAGTG GTCCAAATTT TCCAACAGAA AAGGAATTAC 
ATCTTGGATA ATGCCAATGG AGCTGAATGG ATTATCTACA CAACAAGTAT CATTTTTGTG 
TCACCCTTAT TCATTGGTAT ACCAGCTTAT GTGCAGTGGC AATGTGGAGC GATTGCTATA 
TACTTCTACT GGATGAACTT CTTACTGTAT CTCCAAAGAT TTGAAAATTG TGGAATTTTC 
ATCGTTATGT TGGAGGTAAT TATGAAAACT CTGTTGAGGT CTACAGTCGT GTTTGTCTTT 
CTACTTTTGG CTTTTGGCCT CAGTTTCTAC GTCCTCCTGA GTATACAGGA TGCTTTCAGC 
TCTCCATTGC TTTCTATAAT CCAGACCTTC AGCATGATGC TAGGAGATAT TAATTACCGT 
GATGCCTTCC TAGAACCATT TTTGAGAAAT GAATTGGTAT ATCCAGTTCT GTCTTTTGCA 
CAGCTTATTG CCTTCACAAT GTTTGTCCCA ATTGTGCTCA TGAATTTACT TATTGGGTTG 
GCAGTTGGTG ACATTGCAGA AGTCCAGAAG CACGCATTGT TGAAGAGGAT TGCTATGCAG 
GTGGAACTTC ATACCAGCTT AGAGAAGAAG CTGCCACTGT GGTTCCTACG CAAAGTAGAT 
CAGAAATCCA TCATCGTGTA TCCCAACAGA CCCAGATGTA CAGGGTTATT AGCTATATTT 
CATTTTATTT TTTATACCCA AGAAGAAAGG CAGGAAATTC CAAATGTTGA CACATCTCTG 
GAAACAGAAA TATTGAAGCA GAAATATCGG CTGAAGGATC TCACTTCTCT TGTGGAGAAA 
CAGCATGAGC TTATTAAACT CATCATTCAG AAGATGGAAA TCATCTCTGA GACAGAGGAT 
GAAGACAACC ATCATTCTTT TCAAGACAGG TTTAAAAAAG AGCAGTTAGA ACAAAGGAAT 
AGCAAATGGA ATTCTGTGTT GAAAGCAGTA AAGGCAAAAA CACACTGCCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026920194.1
CDS	297..3650
Translation	MRRSLRKMLSPGGKQEPQGAVYQGVENGMDGRKDSLKVVFEGSTCRFNLMKKRKKLSKYDDVNASPLHHAAEEGQVQLMGMIINDSSFEVLNVMDDYGNTPLHWATEKNQVESVKFLLSKGANPNLRNSNMLAPLHIAVQGLHNEVMKVLTEHNNTNINLEGENGNTAVIIACAKDNSEALQILLNKGAKPCKANKWGCFPIHQAAFSGAKKCMEIILKFGEEHGYSRESHINFVNNAKASPLHMAVQSGDLEMIKMCLDNGAQLDLMENGKCTPLHFAATQGATEIVKLMISSYSGSSDIVNAVDGNQETLLHRASLFDHHELADYLISVGADINSTDSEGRSPLILATASASWNTVNLLLSKGAHVDIKDHLGRNFLHLTVQQPHGLKNLRPEFMQMQHIKKLVMDEDDDGCTPLHYACRHGIPVSVDNLLDFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDMNDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHSGWTALHHASLGGYTQTMKVILDTNMKCTDQLDEEGNTALHFAAREGHAKAVALLLSYNADIVLNKQQASFLHVAIHNKRKDVVLTTIKNKRWEECLKVFSHYSPSNKCPVMEMVEYLPECMKVLLDCCMMPSKRDKSCQDYYIEYNFRYLQCPLEFTKKVTPAQDVTYEPLTTLNAMVQHNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLLPMTFLVVSIKPGMAFNSTGIINETSDHSEILDTKNSYSIKVCMILVFLSSIFGYCKEVVQIFQQKRNYILDNANGAEWIIYTTSIIFVSPLFIGIPAYVQWQCGAIAIYFYWMNFLLYLQRFENCGIFIVMLEVIMKTLLRSTVVFVFLLLAFGLSFYVLLSIQDAFSSPLLSIIQTFSMMLGDINYRDAFLEPFLRNELVYPVLSFAQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHALLKRIAMQVELHTSLEKKLPLWFLRKVDQKSIIVYPNRPRCTGLLAIFHFIFYTQEERQEIPNVDTSLETEILKQKYRLKDLTSLVEKQHELIKLIIQKMEIISETEDEDNHHSFQDRFKKEQLEQRNSKWNSVLKAVKAKTHCP

Target ORF information:

RefSeq Version	XM_027064393.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus transient receptor potential cation channel subfamily A member 1 (TRPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027064393.1

ORF Insert Sequence:

ATGAGGCGCA GCCTGAGGAA GATGCTGAGC CCTGGGGGTA AGCAGGAGCC CCAGGGCGCA 
GTCTACCAGG GCGTGGAGAA CGGCATGGAC GGCCGCAAGG ACTCGCTGAA GGTGGTTTTT 
GAAGGAAGTA CATGTAGATT TAACTTGATG AAGAAACGAA AGAAGTTAAG CAAATATGAT 
GATGTGAACG CATCCCCTTT GCACCATGCT GCAGAAGAAG GTCAAGTGCA GCTGATGGGG 
ATGATCATCA ATGACTCCTC TTTTGAAGTG CTGAATGTGA TGGATGATTA TGGAAATACC 
CCTCTGCACT GGGCCACAGA AAAAAACCAA GTTGAAAGTG TTAAGTTTCT TCTTAGCAAA 
GGAGCCAACC CAAACCTCCG AAACAGCAAC ATGTTGGCTC CTCTTCACAT CGCTGTGCAG 
GGCTTACACA ATGAGGTGAT GAAGGTCTTG ACTGAGCACA ACAACACTAA TATTAATTTG 
GAAGGAGAAA ATGGAAACAC AGCTGTGATA ATCGCATGCG CCAAAGATAA TAGTGAAGCA 
CTGCAAATCT TGTTAAATAA AGGAGCTAAG CCATGTAAGG CAAATAAATG GGGATGTTTC 
CCTATTCATC AGGCTGCATT CTCAGGTGCC AAAAAATGCA TGGAAATAAT ATTAAAGTTT 
GGTGAGGAAC ATGGGTATAG CAGAGAGTCT CATATTAACT TTGTGAATAA TGCAAAAGCT 
AGTCCCCTCC ACATGGCGGT TCAAAGTGGT GACTTGGAAA TGATTAAAAT GTGCTTGGAC 
AATGGCGCAC AGTTGGATCT GATGGAAAAT GGGAAGTGCA CACCCCTTCA CTTTGCTGCC 
ACCCAGGGAG CCACTGAGAT TGTTAAATTG ATGATATCTT CCTATTCTGG TAGCAGTGAT 
ATCGTCAATG CAGTGGATGG AAATCAGGAG ACACTCCTTC ACAGAGCCTC GTTATTTGAT 
CACCATGAAC TGGCAGACTA CTTAATTTCA GTGGGAGCAG ATATCAATAG CACTGATTCG 
GAAGGACGTT CTCCACTTAT ATTAGCAACT GCTTCTGCAT CTTGGAATAC TGTAAATTTG 
CTACTGTCTA AAGGTGCCCA TGTAGACATA AAAGATCATC TTGGACGTAA TTTTTTGCAT 
TTGACTGTAC AGCAACCTCA TGGATTAAAA AATCTGCGAC CTGAGTTTAT GCAGATGCAA 
CATATTAAAA AGCTGGTAAT GGATGAAGAT GATGACGGGT GTACTCCTCT ACATTATGCA 
TGTAGACACG GGATTCCTGT CTCTGTAGAT AACCTTCTTG ACTTTAATGT GTCCATTCAT 
TCCAAAAGCA AAGATAAGAA ATCACCCTTA CATTTTGCAG CCAGTTATGG GCGTATCAAC 
ACCTGTCAGA GGCTTCTGCA AGATATGAAT GACACGAGGC TTTTGAATGA AGGTGACCTC 
CATGGAATGA CGCCTTTGCA CCTGGCTGCA AAAAATGGGC ATGATAAGGT AGTTCAACTT 
CTTCTGAAAA AAGGTGCATT ATTTCTCAGT GACCACAGTG GCTGGACTGC TTTGCATCAT 
GCTTCCTTGG GCGGGTACAC TCAGACCATG AAGGTCATTC TTGACACTAA CATGAAGTGC 
ACAGATCAGC TAGATGAAGA AGGGAACACT GCACTGCACT TTGCTGCAAG GGAAGGCCAT 
GCCAAAGCCG TCGCACTTCT TTTGAGTTAT AATGCTGACA TCGTCCTGAA CAAGCAGCAG 
GCCTCCTTTT TGCATGTTGC AATTCACAAT AAGAGGAAGG ACGTGGTTCT TACGACCATC 
AAGAACAAAA GATGGGAAGA ATGCCTTAAG GTTTTTAGTC ATTATTCTCC AAGCAATAAA 
TGTCCAGTTA TGGAAATGGT AGAATACCTC CCTGAATGCA TGAAGGTCCT TTTAGATTGT 
TGCATGATGC CCTCCAAAAG AGACAAGTCC TGCCAAGACT ATTATATTGA GTATAATTTC 
AGGTATCTTC AGTGTCCATT AGAATTTACC AAGAAAGTAA CACCTGCACA GGATGTTACA 
TATGAGCCTC TTACAACCCT CAACGCAATG GTACAACATA ACCGCATAGA GCTTCTCAAT 
CATCCTGTAT GTAAAGAATA TTTACTTATG AAATGGTTGG CTTATGGGTT TAGAGCCCAT 
ATGATGAACC TAGGATCTTA CTGTCTTGGT CTCCTGCCTA TGACCTTTCT TGTTGTCAGT 
ATAAAGCCAG GTATGGCTTT CAACTCAACT GGAATCATCA ATGAAACTAG TGACCATTCA 
GAAATATTAG ACACCAAGAA TTCCTATTCT ATAAAAGTTT GTATGATTTT AGTTTTTTTA 
TCAAGTATAT TTGGATACTG CAAAGAAGTG GTCCAAATTT TCCAACAGAA AAGGAATTAC 
ATCTTGGATA ATGCCAATGG AGCTGAATGG ATTATCTACA CAACAAGTAT CATTTTTGTG 
TCACCCTTAT TCATTGGTAT ACCAGCTTAT GTGCAGTGGC AATGTGGAGC GATTGCTATA 
TACTTCTACT GGATGAACTT CTTACTGTAT CTCCAAAGAT TTGAAAATTG TGGAATTTTC 
ATCGTTATGT TGGAGGTAAT TATGAAAACT CTGTTGAGGT CTACAGTCGT GTTTGTCTTT 
CTACTTTTGG CTTTTGGCCT CAGTTTCTAC GTCCTCCTGA GTATACAGGA TGCTTTCAGC 
TCTCCATTGC TTTCTATAAT CCAGACCTTC AGCATGATGC TAGGAGATAT TAATTACCGT 
GATGCCTTCC TAGAACCATT TTTGAGAAAT GAATTGGTAT ATCCAGTTCT GTCTTTTGCA 
CAGCTTATTG CCTTCACAAT GTTTGTCCCA ATTGTGCTCA TGAATTTACT TATTGGGTTG 
GCAGTTGGTG ACATTGCAGA AGTCCAGAAG CACGCATTGT TGAAGAGGAT TGCTATGCAG 
GTGGAACTTC ATACCAGCTT AGAGAAGAAG CTGCCACTGT GGTTCCTACG CAAAGTAGAT 
CAGAAATCCA TCATCGTGTA TCCCAACAGA CCCAGATGTA CAGGGTTATT AGCTATATTT 
CATTTTATTT TTTATACCCA AGAAGAAAGG CAGGAAATTC CAAATGTTGA CACATCTCTG 
GAAACAGAAA TATTGAAGCA GAAATATCGG CTGAAGGATC TCACTTCTCT TGTGGAGAAA 
CAGCATGAGC TTATTAAACT CATCATTCAG AAGATGGAAA TCATCTCTGA GACAGAGGAT 
GAAGACAACC ATCATTCTTT TCAAGACAGG TTTAAAAAAG AGCAGTTAGA ACAAAGGAAT 
AGCAAATGGA ATTCTGTGTT GAAAGCAGTA AAGGCAAAAA CACACTGCCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPA1 ( XM_015064743.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014920229.1 Acinonyx jubatus transient receptor potential cation channel, subfamily A, member 1 (TRPA1), mRNA.

Price & Availability↑

CloneID	OAc40129
Clone ID Related Accession (Same CDS sequence)	XM_015064743.1
Accession Version	XM_015064743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	transient receptor potential cation channel subfamily A member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015130784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 21% of CDS bases ##RefSeq-Attributes-END##

ATGGCCGCAA GTGCCCGAGC ACTTAGAACT GCCTTAGCAG ATTCAGCTAA GACAGTGCAT 
CTTCACCCGG GCAGTGACCG CTTGCTTAGG AAGCCCCACC TGCTCGGTGA GCTGCTACAG 
TCTCATCTGT GGAGACTCGC ACTCAAGCTG GGGCCCTTCC CAAGTCCTTC TCAGCCCCTT 
GCTGAGGCCC AAGCTCCTAT AAGGCCGGCA AAATCTGAGC GCCACTTTCT CCAGTCTCCA 
AAGCACAGAC CCACTTCCAC ACAGTCGCTG CTTGTACAGG CAACTGACGT TCTACCCTTG 
GATGCCTGGC AACAAGTAGG TGGCAACGAT AGCCTGGTGG GTTTCCAGAA AGGCCCAAGA 
GCCACTCAAT ATGATGGCAG GAGGCAGCAG GACACATGGT CAGCTCATGC AGCTGCTGCC 
TTTACTGGGA ACCTCCAGGG GTGCGTTGCC GCGGTCGCAG GTCGGACAGG CGAGTCGTGC 
GTGCTCTGGG CCCGAAGCGA CGAGCGGGGG ACAGTCTCGG CGCGAACACC CCTTCGCCGC 
GCGCGGACAA GAGGTCTGTG TCCCTGCCTC TTCCCGGTGT CCCTCCTGCT TCCTGCGCTG 
CAAGCGGCCC CGGGGCCGAA GAACCCCTTC CAGAAGTTCT CTCGGCCTCC GGGAGTGGGG 
GCTTCTGGAA GGACTGTGAA AGAGGAGGCC GTTGAACGCA GGGTAGGTGG TCTTCGCGAG 
ACCCCGAGGG CTAGGACGGG TTTCTGTTGT TTGCGCGGTT CCCGGGACAC GCTGACGTCC 
GGAGAGTCAG GGCGCAGTCT GAGCGCCGAG GCTGTTGGGG GCTCTGCAAA CCCGCCCCTG 
GCTGTCTTCA CGTGGTCGGC GGGAAGAAGG GTGGTTTTTG AAGGAAGTAC ATGTAGATTT 
AACTTGATGA AGAAACGAAA GAAGTTAAGC AAATATGATG ATGTGAACGC ATCCCCTTTG 
CACCATGCTG CAGAAGAAGG TCAAGTGCAG CTGATGGGGA TGATCATCAA TGACTCCTCT 
TTTGAAGTGC TGAATGTGAC GGATGATTAT GGAAATACCC CTCTGCACTG GGCCACAGAA 
AAAAACCAAG TTGAAAGTGT TAAGTTTCTT CTTAGCAAAG GAGCCAACCC AAACCTCCGA 
AACAGCAACA TGTTGGCTCC TCTTCACATC GCTGTGCAGG GCTTACACAA TGAGGTGATG 
AAGGTCTTGA CTGAGCACAA CAACACTAAT ATTAATTTGG AAGGAGAAAA TGGAAACACA 
GCTGTGATAA TCGCATGCGC CAAAGATAAT AGTGAAGCAC TGCAAATCTT GTTAAATAAA 
GGAGCTAAGC CATGTAAGGC AAATAAATGG GGATGTTTCC CTATTCATCA GGCTGCATTC 
TCAGGTGCCA AAAAATGCAT GGAAATAATA TTAAAGTTTG GTGAGGAACA TGGGTATAGC 
AGAGAGTCTC ATATTAACTT TGTGAATAAT GCAAAAGCTA GTCCCCTCCA CATGGCGGTT 
CAAAGTGGTG ACTTGGAAAT GATTAAAATG TGCTTGGACA ATGGCGCACA GTTGGATCTG 
ATGGAAAATG GGAAGTGCAC ACCCCTTCAC TTTGCTGCCA CCCAGGGAGC CACTGAGATT 
GTTAAATTGA TGATATCTTC CTATTCTGGT AGCAGTGATA TCGTCAATGC AGTGGATGGA 
AATCAGGAGA CACTCCTTCA CAGAGCCTCG TTATTTGATC ACCATGAACT GGCAGACTAC 
TTAATTTCAG TGGGAGCAGA TATCAATAGC ACTGATTCGG AAGGACGTTC TCCACTTATA 
TTAGCAACTG CTTCTGCATC TTGGAATACT GTAAATTTGC TACTGTCTAA AGGTGCCCAT 
GTAGACATAA AAGATCATCT TGGACGTAAT TTTTTGCATT TGACTGTACA GCAACCTCAT 
GGATTAAAAA ATCTGCGACC TGAGTTTATG CAGATGCAAC ATATTAAAAA GCTGGTAATG 
GATGAAGATG ATGACGGGTG TACTCCTCTA CATTATGCAT GTAGACACGG GATTCCTGTC 
TCTGTAGATA ACCTTCTTGA CTTTAATGTG TCCATTCATT CCAAAAGCAA AGATAAGAAA 
TCACCCTTAC ATTTTGCAGC CAGTTATGGG CGTATCAACA CCTGTCAGAG GCTTCTGCAA 
GATATGAATG ACACGAGGCT TTTGAATGAA GGTGACCTCC ATGGAATGAC GCCTTTGCAC 
CTGGCTGCAA AAAATGGGCA TGATAAGGTA GTTCAACTTC TTCTGAAAAA AGGTGCATTA 
TTTCTCAGTG ACCACAGTGG CTGGACTGCT TTGCATCATG CTTCCTTGGG CGGGTACACT 
CAGACCATGA AGGTCATTCT TGACACTAAC ATGAAGTGCA CAGATCAGCT AGATGAAGAA 
GGGAACACTG CACTGCACTT TGCTGCAAGG GAAGGCCATG CCAAAGCCGT CGCACTTCTT 
TTGAGTTATA ATGCTGACAT CGTCCTGAAC AAGCAGCAGG CCTCCTTTTT GCATGTTGCA 
ATTCACAATA AGAGGAAGGA CGTGGTTCTT ACGACCATCA AGAACAAAAG ATGGGAAGAA 
TGCCTTAAGG TTTTTAGTCA TTATTCTCCA AGCAATAAAT GTCCAGTTAT GGAAATGGTA 
GAATACCTCC CTGAATGCAT GAAGGTCCTT TTAGATTGTT GCATGATGCC CTCCAAAAGA 
GACAAGTCCT GCCAAGACTA TTATATTGAG TATAATTTCA GGTATCTTCA GTGTCCATTA 
GAATTTACCA AGAAAGTAAC ACCTGCACAG GATGTTACAT ATGAGCCTCT TACAACCCTC 
AACGCAATGG TACAACATAA CCGCATAGAG CTTCTCAATC ATCCTGTATG TAAAGAATAT 
TTACTTATGA AATGGTTGGC TTATGGGTTT AGAGCCCATA TGATGAACCT AGGATCTTAC 
TGTCTTGGTC TCCTGCCTAT GACCTTTCTT GTTGTCAGTA TAAAGCCAGG TATGGCTTTC 
AACTCAACTG GAATCATCAA TGAAACTAGT GACCATTCAG AAATATTAGA CACCAAGAAT 
TCCTATTCTA TAAAAGTTTG TATGATTTTA GTTTTTTTAT CAAGTATATT TGGATACTGC 
AAAGAAGTGG TCCAAATTTT CCAACAGAAA AGGAATTACA TCTTGGATAA TGCCAATGGA 
GCTGAATGGA TTATCTACAC AACAAGTATC ATTTTTGTGT CACCCTTATT CATTGGTATA 
CCAGCTTATG TGCAGTGGCA ATGTGGAGCG ATTGCTATAT ACTTCTACTG GATGAACTTC 
TTACTGTATC TCCAAAGATT TGAAAATTGT GGAATTTTCA TCGTTATGTT GGAGGTAATT 
ATGAAAACTC TGTTGAGGTC TACAGTCGTG TTTGTCTTTC TACTTTTGGC TTTTGGCCTC 
AGTTTCTACG TCCTCCTGAG TATACAGGAT GCTTTCAGCT CTCCATTGCT TTCTATAATC 
CAGACCTTCA GCATGATGCT AGGAGATATT AATTACCGTG ATGCCTTCCT AGAACCATTT 
TTGAGAAATG AATTGGTATA TCCAGTTCTG TCTTTTGCAC AGCTTATTGC CTTCACAATG 
TTTGTCCCAA TTGTGCTCAT GAATTTACTT ATTGGGTTGG CAGTTGGTGA CATTGCAGAA 
GTCCAGAAGC ACGCATTGTT GAAGAGGATT GCTATGCAGG TGGAACTTCA TACCAGCTTA 
GAGAAGAAGC TGCCACTGTG GTTCCTACGC AAAGTAGATC AGAAATCCAT CATCGTGTAT 
CCCAACAGAC CCAGATGTAC AGGGTTATTA GCTATATTTC ATTTTATTTT TTATACCCAA 
GAAGAAAGGC AGGAAATTCC AAATGTTGAC ACATCTCTGG AAACAGAAAT ATTGAAGCAG 
AAATATCGGC TGAAGGATCT CACTTCTCTT GTGGAGAAAC AGCATGAGCT TATTAAACTC 
ATCATTCAGA AGATGGAAAT CATCTCTGAG ACAGAGGATG AAGACAACCA TCATTCTTTT 
CAAGACAGGT TTAAAAAAGA GCAGTTAGAA CAAAGGAATA GCAAATGGAA TTCTGTGTTG 
AAAGCAGTAA AGGCAAAAAC ACACTGCCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014920229.1
CDS	1..4113
Translation	MAASARALRTALADSAKTVHLHPGSDRLLRKPHLLGELLQSHLWRLALKLGPFPSPSQPLAEAQAPIRPAKSERHFLQSPKHRPTSTQSLLVQATDVLPLDAWQQVGGNDSLVGFQKGPRATQYDGRRQQDTWSAHAAAAFTGNLQGCVAAVAGRTGESCVLWARSDERGTVSARTPLRRARTRGLCPCLFPVSLLLPALQAAPGPKNPFQKFSRPPGVGASGRTVKEEAVERRVGGLRETPRARTGFCCLRGSRDTLTSGESGRSLSAEAVGGSANPPLAVFTWSAGRRVVFEGSTCRFNLMKKRKKLSKYDDVNASPLHHAAEEGQVQLMGMIINDSSFEVLNVTDDYGNTPLHWATEKNQVESVKFLLSKGANPNLRNSNMLAPLHIAVQGLHNEVMKVLTEHNNTNINLEGENGNTAVIIACAKDNSEALQILLNKGAKPCKANKWGCFPIHQAAFSGAKKCMEIILKFGEEHGYSRESHINFVNNAKASPLHMAVQSGDLEMIKMCLDNGAQLDLMENGKCTPLHFAATQGATEIVKLMISSYSGSSDIVNAVDGNQETLLHRASLFDHHELADYLISVGADINSTDSEGRSPLILATASASWNTVNLLLSKGAHVDIKDHLGRNFLHLTVQQPHGLKNLRPEFMQMQHIKKLVMDEDDDGCTPLHYACRHGIPVSVDNLLDFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDMNDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHSGWTALHHASLGGYTQTMKVILDTNMKCTDQLDEEGNTALHFAAREGHAKAVALLLSYNADIVLNKQQASFLHVAIHNKRKDVVLTTIKNKRWEECLKVFSHYSPSNKCPVMEMVEYLPECMKVLLDCCMMPSKRDKSCQDYYIEYNFRYLQCPLEFTKKVTPAQDVTYEPLTTLNAMVQHNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLLPMTFLVVSIKPGMAFNSTGIINETSDHSEILDTKNSYSIKVCMILVFLSSIFGYCKEVVQIFQQKRNYILDNANGAEWIIYTTSIIFVSPLFIGIPAYVQWQCGAIAIYFYWMNFLLYLQRFENCGIFIVMLEVIMKTLLRSTVVFVFLLLAFGLSFYVLLSIQDAFSSPLLSIIQTFSMMLGDINYRDAFLEPFLRNELVYPVLSFAQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHALLKRIAMQVELHTSLEKKLPLWFLRKVDQKSIIVYPNRPRCTGLLAIFHFIFYTQEERQEIPNVDTSLETEILKQKYRLKDLTSLVEKQHELIKLIIQKMEIISETEDEDNHHSFQDRFKKEQLEQRNSKWNSVLKAVKAKTHCP

Target ORF information:

RefSeq Version	XM_015064743.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus transient receptor potential cation channel, subfamily A, member 1 (TRPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015064743.1

ORF Insert Sequence:

ATGGCCGCAA GTGCCCGAGC ACTTAGAACT GCCTTAGCAG ATTCAGCTAA GACAGTGCAT 
CTTCACCCGG GCAGTGACCG CTTGCTTAGG AAGCCCCACC TGCTCGGTGA GCTGCTACAG 
TCTCATCTGT GGAGACTCGC ACTCAAGCTG GGGCCCTTCC CAAGTCCTTC TCAGCCCCTT 
GCTGAGGCCC AAGCTCCTAT AAGGCCGGCA AAATCTGAGC GCCACTTTCT CCAGTCTCCA 
AAGCACAGAC CCACTTCCAC ACAGTCGCTG CTTGTACAGG CAACTGACGT TCTACCCTTG 
GATGCCTGGC AACAAGTAGG TGGCAACGAT AGCCTGGTGG GTTTCCAGAA AGGCCCAAGA 
GCCACTCAAT ATGATGGCAG GAGGCAGCAG GACACATGGT CAGCTCATGC AGCTGCTGCC 
TTTACTGGGA ACCTCCAGGG GTGCGTTGCC GCGGTCGCAG GTCGGACAGG CGAGTCGTGC 
GTGCTCTGGG CCCGAAGCGA CGAGCGGGGG ACAGTCTCGG CGCGAACACC CCTTCGCCGC 
GCGCGGACAA GAGGTCTGTG TCCCTGCCTC TTCCCGGTGT CCCTCCTGCT TCCTGCGCTG 
CAAGCGGCCC CGGGGCCGAA GAACCCCTTC CAGAAGTTCT CTCGGCCTCC GGGAGTGGGG 
GCTTCTGGAA GGACTGTGAA AGAGGAGGCC GTTGAACGCA GGGTAGGTGG TCTTCGCGAG 
ACCCCGAGGG CTAGGACGGG TTTCTGTTGT TTGCGCGGTT CCCGGGACAC GCTGACGTCC 
GGAGAGTCAG GGCGCAGTCT GAGCGCCGAG GCTGTTGGGG GCTCTGCAAA CCCGCCCCTG 
GCTGTCTTCA CGTGGTCGGC GGGAAGAAGG GTGGTTTTTG AAGGAAGTAC ATGTAGATTT 
AACTTGATGA AGAAACGAAA GAAGTTAAGC AAATATGATG ATGTGAACGC ATCCCCTTTG 
CACCATGCTG CAGAAGAAGG TCAAGTGCAG CTGATGGGGA TGATCATCAA TGACTCCTCT 
TTTGAAGTGC TGAATGTGAC GGATGATTAT GGAAATACCC CTCTGCACTG GGCCACAGAA 
AAAAACCAAG TTGAAAGTGT TAAGTTTCTT CTTAGCAAAG GAGCCAACCC AAACCTCCGA 
AACAGCAACA TGTTGGCTCC TCTTCACATC GCTGTGCAGG GCTTACACAA TGAGGTGATG 
AAGGTCTTGA CTGAGCACAA CAACACTAAT ATTAATTTGG AAGGAGAAAA TGGAAACACA 
GCTGTGATAA TCGCATGCGC CAAAGATAAT AGTGAAGCAC TGCAAATCTT GTTAAATAAA 
GGAGCTAAGC CATGTAAGGC AAATAAATGG GGATGTTTCC CTATTCATCA GGCTGCATTC 
TCAGGTGCCA AAAAATGCAT GGAAATAATA TTAAAGTTTG GTGAGGAACA TGGGTATAGC 
AGAGAGTCTC ATATTAACTT TGTGAATAAT GCAAAAGCTA GTCCCCTCCA CATGGCGGTT 
CAAAGTGGTG ACTTGGAAAT GATTAAAATG TGCTTGGACA ATGGCGCACA GTTGGATCTG 
ATGGAAAATG GGAAGTGCAC ACCCCTTCAC TTTGCTGCCA CCCAGGGAGC CACTGAGATT 
GTTAAATTGA TGATATCTTC CTATTCTGGT AGCAGTGATA TCGTCAATGC AGTGGATGGA 
AATCAGGAGA CACTCCTTCA CAGAGCCTCG TTATTTGATC ACCATGAACT GGCAGACTAC 
TTAATTTCAG TGGGAGCAGA TATCAATAGC ACTGATTCGG AAGGACGTTC TCCACTTATA 
TTAGCAACTG CTTCTGCATC TTGGAATACT GTAAATTTGC TACTGTCTAA AGGTGCCCAT 
GTAGACATAA AAGATCATCT TGGACGTAAT TTTTTGCATT TGACTGTACA GCAACCTCAT 
GGATTAAAAA ATCTGCGACC TGAGTTTATG CAGATGCAAC ATATTAAAAA GCTGGTAATG 
GATGAAGATG ATGACGGGTG TACTCCTCTA CATTATGCAT GTAGACACGG GATTCCTGTC 
TCTGTAGATA ACCTTCTTGA CTTTAATGTG TCCATTCATT CCAAAAGCAA AGATAAGAAA 
TCACCCTTAC ATTTTGCAGC CAGTTATGGG CGTATCAACA CCTGTCAGAG GCTTCTGCAA 
GATATGAATG ACACGAGGCT TTTGAATGAA GGTGACCTCC ATGGAATGAC GCCTTTGCAC 
CTGGCTGCAA AAAATGGGCA TGATAAGGTA GTTCAACTTC TTCTGAAAAA AGGTGCATTA 
TTTCTCAGTG ACCACAGTGG CTGGACTGCT TTGCATCATG CTTCCTTGGG CGGGTACACT 
CAGACCATGA AGGTCATTCT TGACACTAAC ATGAAGTGCA CAGATCAGCT AGATGAAGAA 
GGGAACACTG CACTGCACTT TGCTGCAAGG GAAGGCCATG CCAAAGCCGT CGCACTTCTT 
TTGAGTTATA ATGCTGACAT CGTCCTGAAC AAGCAGCAGG CCTCCTTTTT GCATGTTGCA 
ATTCACAATA AGAGGAAGGA CGTGGTTCTT ACGACCATCA AGAACAAAAG ATGGGAAGAA 
TGCCTTAAGG TTTTTAGTCA TTATTCTCCA AGCAATAAAT GTCCAGTTAT GGAAATGGTA 
GAATACCTCC CTGAATGCAT GAAGGTCCTT TTAGATTGTT GCATGATGCC CTCCAAAAGA 
GACAAGTCCT GCCAAGACTA TTATATTGAG TATAATTTCA GGTATCTTCA GTGTCCATTA 
GAATTTACCA AGAAAGTAAC ACCTGCACAG GATGTTACAT ATGAGCCTCT TACAACCCTC 
AACGCAATGG TACAACATAA CCGCATAGAG CTTCTCAATC ATCCTGTATG TAAAGAATAT 
TTACTTATGA AATGGTTGGC TTATGGGTTT AGAGCCCATA TGATGAACCT AGGATCTTAC 
TGTCTTGGTC TCCTGCCTAT GACCTTTCTT GTTGTCAGTA TAAAGCCAGG TATGGCTTTC 
AACTCAACTG GAATCATCAA TGAAACTAGT GACCATTCAG AAATATTAGA CACCAAGAAT 
TCCTATTCTA TAAAAGTTTG TATGATTTTA GTTTTTTTAT CAAGTATATT TGGATACTGC 
AAAGAAGTGG TCCAAATTTT CCAACAGAAA AGGAATTACA TCTTGGATAA TGCCAATGGA 
GCTGAATGGA TTATCTACAC AACAAGTATC ATTTTTGTGT CACCCTTATT CATTGGTATA 
CCAGCTTATG TGCAGTGGCA ATGTGGAGCG ATTGCTATAT ACTTCTACTG GATGAACTTC 
TTACTGTATC TCCAAAGATT TGAAAATTGT GGAATTTTCA TCGTTATGTT GGAGGTAATT 
ATGAAAACTC TGTTGAGGTC TACAGTCGTG TTTGTCTTTC TACTTTTGGC TTTTGGCCTC 
AGTTTCTACG TCCTCCTGAG TATACAGGAT GCTTTCAGCT CTCCATTGCT TTCTATAATC 
CAGACCTTCA GCATGATGCT AGGAGATATT AATTACCGTG ATGCCTTCCT AGAACCATTT 
TTGAGAAATG AATTGGTATA TCCAGTTCTG TCTTTTGCAC AGCTTATTGC CTTCACAATG 
TTTGTCCCAA TTGTGCTCAT GAATTTACTT ATTGGGTTGG CAGTTGGTGA CATTGCAGAA 
GTCCAGAAGC ACGCATTGTT GAAGAGGATT GCTATGCAGG TGGAACTTCA TACCAGCTTA 
GAGAAGAAGC TGCCACTGTG GTTCCTACGC AAAGTAGATC AGAAATCCAT CATCGTGTAT 
CCCAACAGAC CCAGATGTAC AGGGTTATTA GCTATATTTC ATTTTATTTT TTATACCCAA 
GAAGAAAGGC AGGAAATTCC AAATGTTGAC ACATCTCTGG AAACAGAAAT ATTGAAGCAG 
AAATATCGGC TGAAGGATCT CACTTCTCTT GTGGAGAAAC AGCATGAGCT TATTAAACTC 
ATCATTCAGA AGATGGAAAT CATCTCTGAG ACAGAGGATG AAGACAACCA TCATTCTTTT 
CAAGACAGGT TTAAAAAAGA GCAGTTAGAA CAAAGGAATA GCAAATGGAA TTCTGTGTTG 
AAAGCAGTAA AGGCAAAAAC ACACTGCCCT TAG