LOC110978671 cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110978671
Entrez Gene ID	110978671
Full Name	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
Gene Type	protein-coding
Organism	Acanthaster planci(crown-of-thorns starfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022233881.1	XP_022089573.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
XM_022233960.1	XP_022089652.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like

Pathways from BioSystems
Gene Ontology

The following LOC110978671 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110978671 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc148805	XM_022233881.1 Latest version!	Acanthaster planci Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC110978671), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OAc148805	XM_022233960.1 Latest version!	Acanthaster planci Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC110978671), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110978671 ( XM_022233881.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022089573.1 Acanthaster planci Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC110978671), transcript variant X1, mRNA.

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CloneID	OAc148805
Clone ID Related Accession (Same CDS sequence)	XM_022233881.1 , XM_022233960.1
Accession Version	XM_022233881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACCA TGGTAGTAGC TCCAAAGATA CAACCACCCA CTAATGGGGT CTACATTGTT 
CAAGGGGAGA TGTTGCTAGT TTCTACAGCC ATGAGGCGGA GCTCCAGATG GACATCCCAT 
TTGCCACAGG AAGAGGAACA GGACCCTTTG CTAGGAAGCT TCTCTCGTCT GAAGGAAATC 
TTGAACTCCA TCTCAGATAT AGCAGATATA GAGCCCAATG TGTTTTTATG TCCTTTCCTC 
GATGTCATCC GGTCTGAAGA CACTACTGGA CCAATCACAG GCTTCGCTCT GTCCTCCATC 
AACAAGTTTC TGTCCTATGG AATGCTAGAC ACATCAGTTG AAGGTGCCAC CTCAGGGATT 
GAAAACATAG CGGATGCCGT CACCCACGCA AGGTTTGTTG GTACAGATCC AGGATCCGAC 
GAAGTGGTAC TCATGAAGAT TCTCCAGGTT CTCAGGACTT TACTTCTGAC CCCTGTTGGA 
GCACACCTCT CCAATGAGTC TGTCTGTGAG ATCATGCAGT CTTGCTTCAG AATTTGCTTT 
GAGATGAGAC TAAGTGAGCT TCTACGCCGA TCGGCAGAGC ACACGCTGGT TGATATGGTG 
CAGCTGCTCT TCTCGAGATT GCCACAGTTC AAAGAGGAGA AACTCTCGGG CATGAGAAAG 
TTAAAAATGC GGCCAGGCGG CATAACTGAC GGAAGTCGCC GCCGCAAATC CCGTCCCTCT 
CCCAAAGTCC GTGCCAAGAA GGACCGTCTT GCCACCACAC CAACTGACGA GCCCTCCATG 
CAATCAGGGG GGGCCGCTTC TCCGCCGACA TCCAACACCA ACGCCTTGGG CCCGACAGTG 
ACTGCACCGG TTACAGCGAC ATCTCCAGGA GCAGCATCCG CTACCATGTC TGCTGTCACA 
ACCGTCACCC CCGGCACTGC GACCACTGCC TCCTCGGAAG CAAGCCAGTC TCAGGTCAAT 
CTGCCGTCAA CCCTGGACGT ACCCTCAGAG GCAGACGAGC AGGCCCTGCC GCCTTCGCCA 
TCCCTCGACA GTGGCGTGGA CATGAAGGAT TCGCTCTCAG CCGTGACCCC TTCAGAGAGT 
GACGTCACCC CAACCCAAGG TCAGGTCATC TCCATGCAAG GAGAGCAGCA GGAGGAGCCT 
ACAGAGAACA AGAAGGGTGA TGAGACTGAC CAGATTCTTG ATGTGGAACG ATCCGATTCC 
TTAGTGGATG GTACCGAAGA CACTGAGTCC ATCACAGAGC ATTCTGACAG TGCCTCCATG 
CAAGACTCTG AATACGTCAA TCCCCAGGGG GTCAGGTTCA CACCCCACCA CAGGCATAAA 
GATGGCTCGG GGCCCATTAT ACCCTATGGG CTACCTTGTG TCAGGGAGTT GATGAGATTC 
CTCATCTCCC TCATCAATCC ATATGACAGG CATAACACAG ACTTGATGAT GCACATGGGT 
CTGAACCTTT TGACCATCGC CTTGGAGACG GGATGCGATC ACATTTCTCA TTTTTCTGCT 
CTGCTCAGTC TCATCAAAGA TGACATGTGC AAGAACCTGT TGGCTCTCCT GCAGGCAGAC 
AGGCTGTCGC TCTTCGCAGC CTCCTTGCGT GTCTGCTTCA TGCTCTTTGA GTCCATGAGG 
ACCCATCTCA AGTTCCAGCT GGAGATGTTC CTGCACAAGC TGATGGAGAT CATCATCTCA 
GAGTCAGTCC GCATTCCCTA CGAGCAGCGT GAGATGGCAC TGGACTCCAT TGTGCAGCTG 
TGGCGACTCC CCTGTATGGT GACAGAACTC TATCTGAACT ATGACTGTGA CATCTACTGC 
AGTAATTTAT TTGAAGATCT GACCAAGTTG CTGTCCAAGA ATGCCTTCCC AGTCTCGGGG 
TCTCTTTTCA CCACCCACCT GCTCTCACTG GAGGCCCTGC TGGCTGTGGT AGACAGCATT 
GAGACTAACT GCCACCACCG CATCCTGAGC TCCATGGTGG CTGCCCAGGG CCATGCCAGC 
CCCCCGGACA GGGACAAGGC TGGTGAGTCG GCCGTCACCA TCCGTGTCCA GGACATCTCG 
GGAAAGGAGG ACGGTGACAA GGGGAAGGAG AGAGGAGGGA AGGATGAGGA TGGAGCCAGG 
CAGGTCGGAA TGGGCGTGAC TCCCCCCACC ACAGGCTACG CCATGGCTCA GGTCATGTCG 
GCGAACCGCG ACAAAGAGAT AGACGCAAGC AAGTTGAAGG CCTCAGAGGA CTTCATCGAG 
GGGAGCCCCA ACCGACGAGT CAGCCGCTTC CCTCCCTCAG CCAATCTGCC ATCGCTGGAG 
CAACTCATGC AGATCAAAAA AAAGAAAAAG ATGATCCAGG CGGGAACAGA ACAGTTCAAC 
CTCAAGCCCT CCAAGGGCAT CGCATTCCTG ATAGAACAGG GCATCCTACA CACCCCGCTG 
GACCCCCACG AGGTGGCCAT CTTCTTCAAA GAGAACCCCC GACTGGACAA GAAGATGATT 
GGGGAATACC TCAGCAGGAA GAAGAACGAG AAGGTCTTGG AAGCTTTTTT GAAGTTGTTT 
GTGTTTGAGG GCACCAGGAT TGATGAAGCC CTTAGGATGT ACCTGGAAGC CTTCCGACTG 
CCAGGAGAGG CTCCTGTCAT CCAACATCTC ATGGAGCACT TCTCAGCCTA CTGGCATAAC 
ACCAACCATC AGCCATATGC AAACAGTGAC GCTGCTTTTA CACTAGCCTA CGCAGTCATC 
ATGTTGAATG TGGACCAGCA CAACCATAAT GCCAAGAAGC AGAACATTCC AATGAACGTC 
GAGCAATTTA AGAAGAATCT GAGCAAGGTG AATGGCGGGT CAGATTTTGA TCAATCCATG 
CTGGAAGAAG TCTTCAATGC TATCAAGAGC GAGGAGATTG TGATGCCGGC TGAGCAGACG 
GGCTTAGTCC GTGACAACTA CTTGTGGCGT GTGCTGCTGA AGCGAGGGGC CAGTCAGGAG 
GGTACCTTCC TGCATGCCCA GTGCGGTGAG CTGGACCGTG AGATGTTCCT GCTGGCCTGG 
GGACCCACAG TGGCTGCCCT GTCCTTTGTC TTTGACAAAG GCATGGATGA CACCATCACA 
CAGAAGGCTA TCGCTGGATT CAGGAAATGT GCAATGATCT CAGCCCATTA CGGTTTGACG 
GACGTGTTCG ACAATTTGGT CATCTCCCTC TGCAAGTTCA CCACTCTTCT GAGTTCCATG 
GAGACCCCAG AGAACCTTCC AATCGCCTTT GGCTCCAATG CCAAGGCCCA CCTTGCGGCC 
AAGACAGTCT TTGGCTTGGC CCATCGCCAT GGCGACATCC TAGCCGAAGG CTGGAAGAAC 
CTCCTGGACT GCATGCTGCA GCTGTATCGG GCCAAGCTCC TCCCCATCGA GATGGTAGAG 
GTCCAAGACT TCGTGGATCC TAGCGGCAGG GTCTCCCTCA TAAGAGAGGA GATGCAACCA 
GTCAAGTCTG ATTCGTCCTT ACTTGGCTCA TTCTACTCCT ACTTCACAAC TGCTGAGACA 
TCTACTCAGA AAGGCCCTAC CAATGAAGAT AAAGAGGCCA TTGAGCAAGC CAGGAGCTGT 
ATAGAGGATT GTCACCCAGA GCACCTGATC ACGGAGAGCA AGTTCCTGCG TCAGGAGTCA 
CTGCAGGAGC TGATGAAGGC CCTGACCTTT GCCTCCCAGG GCCCTCAGGC AGCCGACTCA 
CTGGGGATGC AGTACGAGGA GGAGGCGGCA GTCTTTAACT TTGAGCTCCT CATCAGGGTT 
GTCTTGGAAA ACAGGGATCG GGTCGGTACC CTGTGGCAGG GCATCTGTGA TCATCTCTAC 
AGTCTGATTG TATCGCAATC AGAGTACAGC CAGCTGGTTG AGAGGGCCGT GGTGGGCATC 
CTACGACTTG CTATACGCCT GCTGAGGAAG GAAGACATTG CTGCTCAGGT CTTGACATCT 
CTCCGAATGC TGCTGCTGAT GAAGAAGTCG GTCCTGCAGC GCGTCTGTCG CCAGGTGGCC 
TATGGCCTTC ACGAGCTGCT ACGCACCAAC GCTGCCAACA TCCACTCCTC CCAGGACTGG 
TATACGCTCT TTGCTCTGCT GGAGTGCGTT GGGGCTGGGG CAGTGCCCCC TAGCGTCCAC 
ATGGTAGACG GCAAGAGGAA TCTGGAGATA TCAGACACAG GCACCCAGTC AGATAGCGAG 
ATGTCAACCG GCAGTATTAG TGGTGCCTCA GACCAAGGCT ACACGTCTGA CAGCGAACTC 
TTCAAACACA GGCAGCCTCA CCGATCGAGC AGTGAGCCCG ACGTGTCAAC CATGACAGAT 
GACAGAGTCA TTGTGGTGAA CAATGACAGC ACAGTCCGCC ACACCCACAA CCATTCCAAA 
GGCGTTTCCA AGCCCCAGAA CCAGTTTGAG ATAGCCTTGG ATCAGACGCT GAGCCACCAT 
GACACCAAGG CCCTCATGAA GTGCTGCGAG TCATTGGCAT TCCTGGTGCG AGACGCCGCC 
CACGTCACAC CCGCCAACTT TGAGTCTTGC GTCCATGCCA TCAGGAGCTT TGTGGAGGCC 
ACAGTCAATG GGGGGAAACT TAGACACGAT CGACAAAAGA AACCTGAGCC GACAAAAGAC 
AAGAAAAACT CCCGAATGTC ACGGAATCAG AGGAATAAAA AGGGTATGCC CAAGATAAGC 
TCTGTACCCG AAAAGATCTC GCAGATGGCA GACGGGGCAG ACGATGGGGA AGATGATGGA 
CCTCCTGGAG GCTATCACTC ATTGTCTATA CAGTTACTGG ACCTGATGCA CACCCTCCAC 
ACGAGGGCAG CATCCATCTT CAGTTCCTGG GCTGAGGAAG AGAAGAAAGG TGGATCACAG 
ATGACGATTG ATGCAGGAGC AAGTGCATTG TGGGTCAAAT GCTGGTGCCC GCTACTTCAA 
GGCATCGCCC GGCTGTGCTG CGACATACGA CGCCAGGTAC GCATGCAGGC CCTGACCTAC 
CTGCAGCGGG CTCTGCTTGT GCATGACCTG CAGACGCTGT CGGCTGTAGA GTGGGAATCC 
TGCTTCAACA AGGTCCTGTT CCCGTTGCTC GCCAAGCTGC TGGACCAGAT CCACCCTCAG 
GACCCAGTGG GGCTGGAGGA GACACGCATG AGGGCTGCCA CCCTGCTGTG CAAGGTATTC 
TTACAGCATT TGACACCCCT TCTATCGCTG TCTACATTCA CGGCACTGTG GCTCACCATC 
CTGGATTTCA TGGACAAGTA CATGCATGCT GACAAGAGTG ACCTACTTTA TGAGGCCATC 
CCGGAATCCT TGAAGAACAT GCTTTTGGTC ATGGATACAG CAGGCGTCTT TCAAGATGCG 
GCCGGCTCAG AGCGAGGCCA GAACTGCCAG CTCTGGGTCT TCACCTGGGA GCGTATTGAC 
TGCTTCTTGC CAGGCCTCAA GAATGAAGTC TTCAAGCCCC ACGAGCCAGT TGTGAGCAAA 
GAGCCGGCAC CAAGCATGGC CCCTACACCA GATGACATCG TTAGAGATGG CAGTCCAATT 
GAAGAGGAGC AAGTTACTGT CAGTGCAAAA CCAGAAGAAG CAAGCCCAAA ACCTTCAACA 
CAGGATGCCA CCCCCACAAA AGATGCCCTG CCCACTGTAG GAACAGCAGC CACGCCTACA 
ACCACACCCA GCACCATTGA GACCAACCCA GTCACCAGCC AGCCCAACAT CATCCTGCAG 
CCGCCCCTGC CCTACCTGGC CCCCCTTCCT GGGGCCCAAG AGGCCAAGCC GGGCAGTTCT 
GTGCCGTTCC TCCTTAATCC GGCCATCCTG AAAGGCTCGC CTATCCCCAT CGTCGCGCCC 
ATACTCCCGC AGCCCGAATC CTCCACAACG GGAAGCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022089573.1
CDS	306..6107
Translation	MATMVVAPKIQPPTNGVYIVQGEMLLVSTAMRRSSRWTSHLPQEEEQDPLLGSFSRLKEILNSISDIADIEPNVFLCPFLDVIRSEDTTGPITGFALSSINKFLSYGMLDTSVEGATSGIENIADAVTHARFVGTDPGSDEVVLMKILQVLRTLLLTPVGAHLSNESVCEIMQSCFRICFEMRLSELLRRSAEHTLVDMVQLLFSRLPQFKEEKLSGMRKLKMRPGGITDGSRRRKSRPSPKVRAKKDRLATTPTDEPSMQSGGAASPPTSNTNALGPTVTAPVTATSPGAASATMSAVTTVTPGTATTASSEASQSQVNLPSTLDVPSEADEQALPPSPSLDSGVDMKDSLSAVTPSESDVTPTQGQVISMQGEQQEEPTENKKGDETDQILDVERSDSLVDGTEDTESITEHSDSASMQDSEYVNPQGVRFTPHHRHKDGSGPIIPYGLPCVRELMRFLISLINPYDRHNTDLMMHMGLNLLTIALETGCDHISHFSALLSLIKDDMCKNLLALLQADRLSLFAASLRVCFMLFESMRTHLKFQLEMFLHKLMEIIISESVRIPYEQREMALDSIVQLWRLPCMVTELYLNYDCDIYCSNLFEDLTKLLSKNAFPVSGSLFTTHLLSLEALLAVVDSIETNCHHRILSSMVAAQGHASPPDRDKAGESAVTIRVQDISGKEDGDKGKERGGKDEDGARQVGMGVTPPTTGYAMAQVMSANRDKEIDASKLKASEDFIEGSPNRRVSRFPPSANLPSLEQLMQIKKKKKMIQAGTEQFNLKPSKGIAFLIEQGILHTPLDPHEVAIFFKENPRLDKKMIGEYLSRKKNEKVLEAFLKLFVFEGTRIDEALRMYLEAFRLPGEAPVIQHLMEHFSAYWHNTNHQPYANSDAAFTLAYAVIMLNVDQHNHNAKKQNIPMNVEQFKKNLSKVNGGSDFDQSMLEEVFNAIKSEEIVMPAEQTGLVRDNYLWRVLLKRGASQEGTFLHAQCGELDREMFLLAWGPTVAALSFVFDKGMDDTITQKAIAGFRKCAMISAHYGLTDVFDNLVISLCKFTTLLSSMETPENLPIAFGSNAKAHLAAKTVFGLAHRHGDILAEGWKNLLDCMLQLYRAKLLPIEMVEVQDFVDPSGRVSLIREEMQPVKSDSSLLGSFYSYFTTAETSTQKGPTNEDKEAIEQARSCIEDCHPEHLITESKFLRQESLQELMKALTFASQGPQAADSLGMQYEEEAAVFNFELLIRVVLENRDRVGTLWQGICDHLYSLIVSQSEYSQLVERAVVGILRLAIRLLRKEDIAAQVLTSLRMLLLMKKSVLQRVCRQVAYGLHELLRTNAANIHSSQDWYTLFALLECVGAGAVPPSVHMVDGKRNLEISDTGTQSDSEMSTGSISGASDQGYTSDSELFKHRQPHRSSSEPDVSTMTDDRVIVVNNDSTVRHTHNHSKGVSKPQNQFEIALDQTLSHHDTKALMKCCESLAFLVRDAAHVTPANFESCVHAIRSFVEATVNGGKLRHDRQKKPEPTKDKKNSRMSRNQRNKKGMPKISSVPEKISQMADGADDGEDDGPPGGYHSLSIQLLDLMHTLHTRAASIFSSWAEEEKKGGSQMTIDAGASALWVKCWCPLLQGIARLCCDIRRQVRMQALTYLQRALLVHDLQTLSAVEWESCFNKVLFPLLAKLLDQIHPQDPVGLEETRMRAATLLCKVFLQHLTPLLSLSTFTALWLTILDFMDKYMHADKSDLLYEAIPESLKNMLLVMDTAGVFQDAAGSERGQNCQLWVFTWERIDCFLPGLKNEVFKPHEPVVSKEPAPSMAPTPDDIVRDGSPIEEEQVTVSAKPEEASPKPSTQDATPTKDALPTVGTAATPTTTPSTIETNPVTSQPNIILQPPLPYLAPLPGAQEAKPGSSVPFLLNPAILKGSPIPIVAPILPQPESSTTGSS

Target ORF information:

RefSeq Version	XM_022233881.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC110978671), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022233881.1

ORF Insert Sequence:

ATGGCAACCA TGGTAGTAGC TCCAAAGATA CAACCACCCA CTAATGGGGT CTACATTGTT 
CAAGGGGAGA TGTTGCTAGT TTCTACAGCC ATGAGGCGGA GCTCCAGATG GACATCCCAT 
TTGCCACAGG AAGAGGAACA GGACCCTTTG CTAGGAAGCT TCTCTCGTCT GAAGGAAATC 
TTGAACTCCA TCTCAGATAT AGCAGATATA GAGCCCAATG TGTTTTTATG TCCTTTCCTC 
GATGTCATCC GGTCTGAAGA CACTACTGGA CCAATCACAG GCTTCGCTCT GTCCTCCATC 
AACAAGTTTC TGTCCTATGG AATGCTAGAC ACATCAGTTG AAGGTGCCAC CTCAGGGATT 
GAAAACATAG CGGATGCCGT CACCCACGCA AGGTTTGTTG GTACAGATCC AGGATCCGAC 
GAAGTGGTAC TCATGAAGAT TCTCCAGGTT CTCAGGACTT TACTTCTGAC CCCTGTTGGA 
GCACACCTCT CCAATGAGTC TGTCTGTGAG ATCATGCAGT CTTGCTTCAG AATTTGCTTT 
GAGATGAGAC TAAGTGAGCT TCTACGCCGA TCGGCAGAGC ACACGCTGGT TGATATGGTG 
CAGCTGCTCT TCTCGAGATT GCCACAGTTC AAAGAGGAGA AACTCTCGGG CATGAGAAAG 
TTAAAAATGC GGCCAGGCGG CATAACTGAC GGAAGTCGCC GCCGCAAATC CCGTCCCTCT 
CCCAAAGTCC GTGCCAAGAA GGACCGTCTT GCCACCACAC CAACTGACGA GCCCTCCATG 
CAATCAGGGG GGGCCGCTTC TCCGCCGACA TCCAACACCA ACGCCTTGGG CCCGACAGTG 
ACTGCACCGG TTACAGCGAC ATCTCCAGGA GCAGCATCCG CTACCATGTC TGCTGTCACA 
ACCGTCACCC CCGGCACTGC GACCACTGCC TCCTCGGAAG CAAGCCAGTC TCAGGTCAAT 
CTGCCGTCAA CCCTGGACGT ACCCTCAGAG GCAGACGAGC AGGCCCTGCC GCCTTCGCCA 
TCCCTCGACA GTGGCGTGGA CATGAAGGAT TCGCTCTCAG CCGTGACCCC TTCAGAGAGT 
GACGTCACCC CAACCCAAGG TCAGGTCATC TCCATGCAAG GAGAGCAGCA GGAGGAGCCT 
ACAGAGAACA AGAAGGGTGA TGAGACTGAC CAGATTCTTG ATGTGGAACG ATCCGATTCC 
TTAGTGGATG GTACCGAAGA CACTGAGTCC ATCACAGAGC ATTCTGACAG TGCCTCCATG 
CAAGACTCTG AATACGTCAA TCCCCAGGGG GTCAGGTTCA CACCCCACCA CAGGCATAAA 
GATGGCTCGG GGCCCATTAT ACCCTATGGG CTACCTTGTG TCAGGGAGTT GATGAGATTC 
CTCATCTCCC TCATCAATCC ATATGACAGG CATAACACAG ACTTGATGAT GCACATGGGT 
CTGAACCTTT TGACCATCGC CTTGGAGACG GGATGCGATC ACATTTCTCA TTTTTCTGCT 
CTGCTCAGTC TCATCAAAGA TGACATGTGC AAGAACCTGT TGGCTCTCCT GCAGGCAGAC 
AGGCTGTCGC TCTTCGCAGC CTCCTTGCGT GTCTGCTTCA TGCTCTTTGA GTCCATGAGG 
ACCCATCTCA AGTTCCAGCT GGAGATGTTC CTGCACAAGC TGATGGAGAT CATCATCTCA 
GAGTCAGTCC GCATTCCCTA CGAGCAGCGT GAGATGGCAC TGGACTCCAT TGTGCAGCTG 
TGGCGACTCC CCTGTATGGT GACAGAACTC TATCTGAACT ATGACTGTGA CATCTACTGC 
AGTAATTTAT TTGAAGATCT GACCAAGTTG CTGTCCAAGA ATGCCTTCCC AGTCTCGGGG 
TCTCTTTTCA CCACCCACCT GCTCTCACTG GAGGCCCTGC TGGCTGTGGT AGACAGCATT 
GAGACTAACT GCCACCACCG CATCCTGAGC TCCATGGTGG CTGCCCAGGG CCATGCCAGC 
CCCCCGGACA GGGACAAGGC TGGTGAGTCG GCCGTCACCA TCCGTGTCCA GGACATCTCG 
GGAAAGGAGG ACGGTGACAA GGGGAAGGAG AGAGGAGGGA AGGATGAGGA TGGAGCCAGG 
CAGGTCGGAA TGGGCGTGAC TCCCCCCACC ACAGGCTACG CCATGGCTCA GGTCATGTCG 
GCGAACCGCG ACAAAGAGAT AGACGCAAGC AAGTTGAAGG CCTCAGAGGA CTTCATCGAG 
GGGAGCCCCA ACCGACGAGT CAGCCGCTTC CCTCCCTCAG CCAATCTGCC ATCGCTGGAG 
CAACTCATGC AGATCAAAAA AAAGAAAAAG ATGATCCAGG CGGGAACAGA ACAGTTCAAC 
CTCAAGCCCT CCAAGGGCAT CGCATTCCTG ATAGAACAGG GCATCCTACA CACCCCGCTG 
GACCCCCACG AGGTGGCCAT CTTCTTCAAA GAGAACCCCC GACTGGACAA GAAGATGATT 
GGGGAATACC TCAGCAGGAA GAAGAACGAG AAGGTCTTGG AAGCTTTTTT GAAGTTGTTT 
GTGTTTGAGG GCACCAGGAT TGATGAAGCC CTTAGGATGT ACCTGGAAGC CTTCCGACTG 
CCAGGAGAGG CTCCTGTCAT CCAACATCTC ATGGAGCACT TCTCAGCCTA CTGGCATAAC 
ACCAACCATC AGCCATATGC AAACAGTGAC GCTGCTTTTA CACTAGCCTA CGCAGTCATC 
ATGTTGAATG TGGACCAGCA CAACCATAAT GCCAAGAAGC AGAACATTCC AATGAACGTC 
GAGCAATTTA AGAAGAATCT GAGCAAGGTG AATGGCGGGT CAGATTTTGA TCAATCCATG 
CTGGAAGAAG TCTTCAATGC TATCAAGAGC GAGGAGATTG TGATGCCGGC TGAGCAGACG 
GGCTTAGTCC GTGACAACTA CTTGTGGCGT GTGCTGCTGA AGCGAGGGGC CAGTCAGGAG 
GGTACCTTCC TGCATGCCCA GTGCGGTGAG CTGGACCGTG AGATGTTCCT GCTGGCCTGG 
GGACCCACAG TGGCTGCCCT GTCCTTTGTC TTTGACAAAG GCATGGATGA CACCATCACA 
CAGAAGGCTA TCGCTGGATT CAGGAAATGT GCAATGATCT CAGCCCATTA CGGTTTGACG 
GACGTGTTCG ACAATTTGGT CATCTCCCTC TGCAAGTTCA CCACTCTTCT GAGTTCCATG 
GAGACCCCAG AGAACCTTCC AATCGCCTTT GGCTCCAATG CCAAGGCCCA CCTTGCGGCC 
AAGACAGTCT TTGGCTTGGC CCATCGCCAT GGCGACATCC TAGCCGAAGG CTGGAAGAAC 
CTCCTGGACT GCATGCTGCA GCTGTATCGG GCCAAGCTCC TCCCCATCGA GATGGTAGAG 
GTCCAAGACT TCGTGGATCC TAGCGGCAGG GTCTCCCTCA TAAGAGAGGA GATGCAACCA 
GTCAAGTCTG ATTCGTCCTT ACTTGGCTCA TTCTACTCCT ACTTCACAAC TGCTGAGACA 
TCTACTCAGA AAGGCCCTAC CAATGAAGAT AAAGAGGCCA TTGAGCAAGC CAGGAGCTGT 
ATAGAGGATT GTCACCCAGA GCACCTGATC ACGGAGAGCA AGTTCCTGCG TCAGGAGTCA 
CTGCAGGAGC TGATGAAGGC CCTGACCTTT GCCTCCCAGG GCCCTCAGGC AGCCGACTCA 
CTGGGGATGC AGTACGAGGA GGAGGCGGCA GTCTTTAACT TTGAGCTCCT CATCAGGGTT 
GTCTTGGAAA ACAGGGATCG GGTCGGTACC CTGTGGCAGG GCATCTGTGA TCATCTCTAC 
AGTCTGATTG TATCGCAATC AGAGTACAGC CAGCTGGTTG AGAGGGCCGT GGTGGGCATC 
CTACGACTTG CTATACGCCT GCTGAGGAAG GAAGACATTG CTGCTCAGGT CTTGACATCT 
CTCCGAATGC TGCTGCTGAT GAAGAAGTCG GTCCTGCAGC GCGTCTGTCG CCAGGTGGCC 
TATGGCCTTC ACGAGCTGCT ACGCACCAAC GCTGCCAACA TCCACTCCTC CCAGGACTGG 
TATACGCTCT TTGCTCTGCT GGAGTGCGTT GGGGCTGGGG CAGTGCCCCC TAGCGTCCAC 
ATGGTAGACG GCAAGAGGAA TCTGGAGATA TCAGACACAG GCACCCAGTC AGATAGCGAG 
ATGTCAACCG GCAGTATTAG TGGTGCCTCA GACCAAGGCT ACACGTCTGA CAGCGAACTC 
TTCAAACACA GGCAGCCTCA CCGATCGAGC AGTGAGCCCG ACGTGTCAAC CATGACAGAT 
GACAGAGTCA TTGTGGTGAA CAATGACAGC ACAGTCCGCC ACACCCACAA CCATTCCAAA 
GGCGTTTCCA AGCCCCAGAA CCAGTTTGAG ATAGCCTTGG ATCAGACGCT GAGCCACCAT 
GACACCAAGG CCCTCATGAA GTGCTGCGAG TCATTGGCAT TCCTGGTGCG AGACGCCGCC 
CACGTCACAC CCGCCAACTT TGAGTCTTGC GTCCATGCCA TCAGGAGCTT TGTGGAGGCC 
ACAGTCAATG GGGGGAAACT TAGACACGAT CGACAAAAGA AACCTGAGCC GACAAAAGAC 
AAGAAAAACT CCCGAATGTC ACGGAATCAG AGGAATAAAA AGGGTATGCC CAAGATAAGC 
TCTGTACCCG AAAAGATCTC GCAGATGGCA GACGGGGCAG ACGATGGGGA AGATGATGGA 
CCTCCTGGAG GCTATCACTC ATTGTCTATA CAGTTACTGG ACCTGATGCA CACCCTCCAC 
ACGAGGGCAG CATCCATCTT CAGTTCCTGG GCTGAGGAAG AGAAGAAAGG TGGATCACAG 
ATGACGATTG ATGCAGGAGC AAGTGCATTG TGGGTCAAAT GCTGGTGCCC GCTACTTCAA 
GGCATCGCCC GGCTGTGCTG CGACATACGA CGCCAGGTAC GCATGCAGGC CCTGACCTAC 
CTGCAGCGGG CTCTGCTTGT GCATGACCTG CAGACGCTGT CGGCTGTAGA GTGGGAATCC 
TGCTTCAACA AGGTCCTGTT CCCGTTGCTC GCCAAGCTGC TGGACCAGAT CCACCCTCAG 
GACCCAGTGG GGCTGGAGGA GACACGCATG AGGGCTGCCA CCCTGCTGTG CAAGGTATTC 
TTACAGCATT TGACACCCCT TCTATCGCTG TCTACATTCA CGGCACTGTG GCTCACCATC 
CTGGATTTCA TGGACAAGTA CATGCATGCT GACAAGAGTG ACCTACTTTA TGAGGCCATC 
CCGGAATCCT TGAAGAACAT GCTTTTGGTC ATGGATACAG CAGGCGTCTT TCAAGATGCG 
GCCGGCTCAG AGCGAGGCCA GAACTGCCAG CTCTGGGTCT TCACCTGGGA GCGTATTGAC 
TGCTTCTTGC CAGGCCTCAA GAATGAAGTC TTCAAGCCCC ACGAGCCAGT TGTGAGCAAA 
GAGCCGGCAC CAAGCATGGC CCCTACACCA GATGACATCG TTAGAGATGG CAGTCCAATT 
GAAGAGGAGC AAGTTACTGT CAGTGCAAAA CCAGAAGAAG CAAGCCCAAA ACCTTCAACA 
CAGGATGCCA CCCCCACAAA AGATGCCCTG CCCACTGTAG GAACAGCAGC CACGCCTACA 
ACCACACCCA GCACCATTGA GACCAACCCA GTCACCAGCC AGCCCAACAT CATCCTGCAG 
CCGCCCCTGC CCTACCTGGC CCCCCTTCCT GGGGCCCAAG AGGCCAAGCC GGGCAGTTCT 
GTGCCGTTCC TCCTTAATCC GGCCATCCTG AAAGGCTCGC CTATCCCCAT CGTCGCGCCC 
ATACTCCCGC AGCCCGAATC CTCCACAACG GGAAGCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110978671 ( XM_022233960.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022089652.1 Acanthaster planci Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC110978671), transcript variant X2, mRNA.

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CloneID	OAc148805
Clone ID Related Accession (Same CDS sequence)	XM_022233881.1 , XM_022233960.1
Accession Version	XM_022233960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACCA TGGTAGTAGC TCCAAAGATA CAACCACCCA CTAATGGGGT CTACATTGTT 
CAAGGGGAGA TGTTGCTAGT TTCTACAGCC ATGAGGCGGA GCTCCAGATG GACATCCCAT 
TTGCCACAGG AAGAGGAACA GGACCCTTTG CTAGGAAGCT TCTCTCGTCT GAAGGAAATC 
TTGAACTCCA TCTCAGATAT AGCAGATATA GAGCCCAATG TGTTTTTATG TCCTTTCCTC 
GATGTCATCC GGTCTGAAGA CACTACTGGA CCAATCACAG GCTTCGCTCT GTCCTCCATC 
AACAAGTTTC TGTCCTATGG AATGCTAGAC ACATCAGTTG AAGGTGCCAC CTCAGGGATT 
GAAAACATAG CGGATGCCGT CACCCACGCA AGGTTTGTTG GTACAGATCC AGGATCCGAC 
GAAGTGGTAC TCATGAAGAT TCTCCAGGTT CTCAGGACTT TACTTCTGAC CCCTGTTGGA 
GCACACCTCT CCAATGAGTC TGTCTGTGAG ATCATGCAGT CTTGCTTCAG AATTTGCTTT 
GAGATGAGAC TAAGTGAGCT TCTACGCCGA TCGGCAGAGC ACACGCTGGT TGATATGGTG 
CAGCTGCTCT TCTCGAGATT GCCACAGTTC AAAGAGGAGA AACTCTCGGG CATGAGAAAG 
TTAAAAATGC GGCCAGGCGG CATAACTGAC GGAAGTCGCC GCCGCAAATC CCGTCCCTCT 
CCCAAAGTCC GTGCCAAGAA GGACCGTCTT GCCACCACAC CAACTGACGA GCCCTCCATG 
CAATCAGGGG GGGCCGCTTC TCCGCCGACA TCCAACACCA ACGCCTTGGG CCCGACAGTG 
ACTGCACCGG TTACAGCGAC ATCTCCAGGA GCAGCATCCG CTACCATGTC TGCTGTCACA 
ACCGTCACCC CCGGCACTGC GACCACTGCC TCCTCGGAAG CAAGCCAGTC TCAGGTCAAT 
CTGCCGTCAA CCCTGGACGT ACCCTCAGAG GCAGACGAGC AGGCCCTGCC GCCTTCGCCA 
TCCCTCGACA GTGGCGTGGA CATGAAGGAT TCGCTCTCAG CCGTGACCCC TTCAGAGAGT 
GACGTCACCC CAACCCAAGG TCAGGTCATC TCCATGCAAG GAGAGCAGCA GGAGGAGCCT 
ACAGAGAACA AGAAGGGTGA TGAGACTGAC CAGATTCTTG ATGTGGAACG ATCCGATTCC 
TTAGTGGATG GTACCGAAGA CACTGAGTCC ATCACAGAGC ATTCTGACAG TGCCTCCATG 
CAAGACTCTG AATACGTCAA TCCCCAGGGG GTCAGGTTCA CACCCCACCA CAGGCATAAA 
GATGGCTCGG GGCCCATTAT ACCCTATGGG CTACCTTGTG TCAGGGAGTT GATGAGATTC 
CTCATCTCCC TCATCAATCC ATATGACAGG CATAACACAG ACTTGATGAT GCACATGGGT 
CTGAACCTTT TGACCATCGC CTTGGAGACG GGATGCGATC ACATTTCTCA TTTTTCTGCT 
CTGCTCAGTC TCATCAAAGA TGACATGTGC AAGAACCTGT TGGCTCTCCT GCAGGCAGAC 
AGGCTGTCGC TCTTCGCAGC CTCCTTGCGT GTCTGCTTCA TGCTCTTTGA GTCCATGAGG 
ACCCATCTCA AGTTCCAGCT GGAGATGTTC CTGCACAAGC TGATGGAGAT CATCATCTCA 
GAGTCAGTCC GCATTCCCTA CGAGCAGCGT GAGATGGCAC TGGACTCCAT TGTGCAGCTG 
TGGCGACTCC CCTGTATGGT GACAGAACTC TATCTGAACT ATGACTGTGA CATCTACTGC 
AGTAATTTAT TTGAAGATCT GACCAAGTTG CTGTCCAAGA ATGCCTTCCC AGTCTCGGGG 
TCTCTTTTCA CCACCCACCT GCTCTCACTG GAGGCCCTGC TGGCTGTGGT AGACAGCATT 
GAGACTAACT GCCACCACCG CATCCTGAGC TCCATGGTGG CTGCCCAGGG CCATGCCAGC 
CCCCCGGACA GGGACAAGGC TGGTGAGTCG GCCGTCACCA TCCGTGTCCA GGACATCTCG 
GGAAAGGAGG ACGGTGACAA GGGGAAGGAG AGAGGAGGGA AGGATGAGGA TGGAGCCAGG 
CAGGTCGGAA TGGGCGTGAC TCCCCCCACC ACAGGCTACG CCATGGCTCA GGTCATGTCG 
GCGAACCGCG ACAAAGAGAT AGACGCAAGC AAGTTGAAGG CCTCAGAGGA CTTCATCGAG 
GGGAGCCCCA ACCGACGAGT CAGCCGCTTC CCTCCCTCAG CCAATCTGCC ATCGCTGGAG 
CAACTCATGC AGATCAAAAA AAAGAAAAAG ATGATCCAGG CGGGAACAGA ACAGTTCAAC 
CTCAAGCCCT CCAAGGGCAT CGCATTCCTG ATAGAACAGG GCATCCTACA CACCCCGCTG 
GACCCCCACG AGGTGGCCAT CTTCTTCAAA GAGAACCCCC GACTGGACAA GAAGATGATT 
GGGGAATACC TCAGCAGGAA GAAGAACGAG AAGGTCTTGG AAGCTTTTTT GAAGTTGTTT 
GTGTTTGAGG GCACCAGGAT TGATGAAGCC CTTAGGATGT ACCTGGAAGC CTTCCGACTG 
CCAGGAGAGG CTCCTGTCAT CCAACATCTC ATGGAGCACT TCTCAGCCTA CTGGCATAAC 
ACCAACCATC AGCCATATGC AAACAGTGAC GCTGCTTTTA CACTAGCCTA CGCAGTCATC 
ATGTTGAATG TGGACCAGCA CAACCATAAT GCCAAGAAGC AGAACATTCC AATGAACGTC 
GAGCAATTTA AGAAGAATCT GAGCAAGGTG AATGGCGGGT CAGATTTTGA TCAATCCATG 
CTGGAAGAAG TCTTCAATGC TATCAAGAGC GAGGAGATTG TGATGCCGGC TGAGCAGACG 
GGCTTAGTCC GTGACAACTA CTTGTGGCGT GTGCTGCTGA AGCGAGGGGC CAGTCAGGAG 
GGTACCTTCC TGCATGCCCA GTGCGGTGAG CTGGACCGTG AGATGTTCCT GCTGGCCTGG 
GGACCCACAG TGGCTGCCCT GTCCTTTGTC TTTGACAAAG GCATGGATGA CACCATCACA 
CAGAAGGCTA TCGCTGGATT CAGGAAATGT GCAATGATCT CAGCCCATTA CGGTTTGACG 
GACGTGTTCG ACAATTTGGT CATCTCCCTC TGCAAGTTCA CCACTCTTCT GAGTTCCATG 
GAGACCCCAG AGAACCTTCC AATCGCCTTT GGCTCCAATG CCAAGGCCCA CCTTGCGGCC 
AAGACAGTCT TTGGCTTGGC CCATCGCCAT GGCGACATCC TAGCCGAAGG CTGGAAGAAC 
CTCCTGGACT GCATGCTGCA GCTGTATCGG GCCAAGCTCC TCCCCATCGA GATGGTAGAG 
GTCCAAGACT TCGTGGATCC TAGCGGCAGG GTCTCCCTCA TAAGAGAGGA GATGCAACCA 
GTCAAGTCTG ATTCGTCCTT ACTTGGCTCA TTCTACTCCT ACTTCACAAC TGCTGAGACA 
TCTACTCAGA AAGGCCCTAC CAATGAAGAT AAAGAGGCCA TTGAGCAAGC CAGGAGCTGT 
ATAGAGGATT GTCACCCAGA GCACCTGATC ACGGAGAGCA AGTTCCTGCG TCAGGAGTCA 
CTGCAGGAGC TGATGAAGGC CCTGACCTTT GCCTCCCAGG GCCCTCAGGC AGCCGACTCA 
CTGGGGATGC AGTACGAGGA GGAGGCGGCA GTCTTTAACT TTGAGCTCCT CATCAGGGTT 
GTCTTGGAAA ACAGGGATCG GGTCGGTACC CTGTGGCAGG GCATCTGTGA TCATCTCTAC 
AGTCTGATTG TATCGCAATC AGAGTACAGC CAGCTGGTTG AGAGGGCCGT GGTGGGCATC 
CTACGACTTG CTATACGCCT GCTGAGGAAG GAAGACATTG CTGCTCAGGT CTTGACATCT 
CTCCGAATGC TGCTGCTGAT GAAGAAGTCG GTCCTGCAGC GCGTCTGTCG CCAGGTGGCC 
TATGGCCTTC ACGAGCTGCT ACGCACCAAC GCTGCCAACA TCCACTCCTC CCAGGACTGG 
TATACGCTCT TTGCTCTGCT GGAGTGCGTT GGGGCTGGGG CAGTGCCCCC TAGCGTCCAC 
ATGGTAGACG GCAAGAGGAA TCTGGAGATA TCAGACACAG GCACCCAGTC AGATAGCGAG 
ATGTCAACCG GCAGTATTAG TGGTGCCTCA GACCAAGGCT ACACGTCTGA CAGCGAACTC 
TTCAAACACA GGCAGCCTCA CCGATCGAGC AGTGAGCCCG ACGTGTCAAC CATGACAGAT 
GACAGAGTCA TTGTGGTGAA CAATGACAGC ACAGTCCGCC ACACCCACAA CCATTCCAAA 
GGCGTTTCCA AGCCCCAGAA CCAGTTTGAG ATAGCCTTGG ATCAGACGCT GAGCCACCAT 
GACACCAAGG CCCTCATGAA GTGCTGCGAG TCATTGGCAT TCCTGGTGCG AGACGCCGCC 
CACGTCACAC CCGCCAACTT TGAGTCTTGC GTCCATGCCA TCAGGAGCTT TGTGGAGGCC 
ACAGTCAATG GGGGGAAACT TAGACACGAT CGACAAAAGA AACCTGAGCC GACAAAAGAC 
AAGAAAAACT CCCGAATGTC ACGGAATCAG AGGAATAAAA AGGGTATGCC CAAGATAAGC 
TCTGTACCCG AAAAGATCTC GCAGATGGCA GACGGGGCAG ACGATGGGGA AGATGATGGA 
CCTCCTGGAG GCTATCACTC ATTGTCTATA CAGTTACTGG ACCTGATGCA CACCCTCCAC 
ACGAGGGCAG CATCCATCTT CAGTTCCTGG GCTGAGGAAG AGAAGAAAGG TGGATCACAG 
ATGACGATTG ATGCAGGAGC AAGTGCATTG TGGGTCAAAT GCTGGTGCCC GCTACTTCAA 
GGCATCGCCC GGCTGTGCTG CGACATACGA CGCCAGGTAC GCATGCAGGC CCTGACCTAC 
CTGCAGCGGG CTCTGCTTGT GCATGACCTG CAGACGCTGT CGGCTGTAGA GTGGGAATCC 
TGCTTCAACA AGGTCCTGTT CCCGTTGCTC GCCAAGCTGC TGGACCAGAT CCACCCTCAG 
GACCCAGTGG GGCTGGAGGA GACACGCATG AGGGCTGCCA CCCTGCTGTG CAAGGTATTC 
TTACAGCATT TGACACCCCT TCTATCGCTG TCTACATTCA CGGCACTGTG GCTCACCATC 
CTGGATTTCA TGGACAAGTA CATGCATGCT GACAAGAGTG ACCTACTTTA TGAGGCCATC 
CCGGAATCCT TGAAGAACAT GCTTTTGGTC ATGGATACAG CAGGCGTCTT TCAAGATGCG 
GCCGGCTCAG AGCGAGGCCA GAACTGCCAG CTCTGGGTCT TCACCTGGGA GCGTATTGAC 
TGCTTCTTGC CAGGCCTCAA GAATGAAGTC TTCAAGCCCC ACGAGCCAGT TGTGAGCAAA 
GAGCCGGCAC CAAGCATGGC CCCTACACCA GATGACATCG TTAGAGATGG CAGTCCAATT 
GAAGAGGAGC AAGTTACTGT CAGTGCAAAA CCAGAAGAAG CAAGCCCAAA ACCTTCAACA 
CAGGATGCCA CCCCCACAAA AGATGCCCTG CCCACTGTAG GAACAGCAGC CACGCCTACA 
ACCACACCCA GCACCATTGA GACCAACCCA GTCACCAGCC AGCCCAACAT CATCCTGCAG 
CCGCCCCTGC CCTACCTGGC CCCCCTTCCT GGGGCCCAAG AGGCCAAGCC GGGCAGTTCT 
GTGCCGTTCC TCCTTAATCC GGCCATCCTG AAAGGCTCGC CTATCCCCAT CGTCGCGCCC 
ATACTCCCGC AGCCCGAATC CTCCACAACG GGAAGCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022089652.1
CDS	302..6103
Translation	MATMVVAPKIQPPTNGVYIVQGEMLLVSTAMRRSSRWTSHLPQEEEQDPLLGSFSRLKEILNSISDIADIEPNVFLCPFLDVIRSEDTTGPITGFALSSINKFLSYGMLDTSVEGATSGIENIADAVTHARFVGTDPGSDEVVLMKILQVLRTLLLTPVGAHLSNESVCEIMQSCFRICFEMRLSELLRRSAEHTLVDMVQLLFSRLPQFKEEKLSGMRKLKMRPGGITDGSRRRKSRPSPKVRAKKDRLATTPTDEPSMQSGGAASPPTSNTNALGPTVTAPVTATSPGAASATMSAVTTVTPGTATTASSEASQSQVNLPSTLDVPSEADEQALPPSPSLDSGVDMKDSLSAVTPSESDVTPTQGQVISMQGEQQEEPTENKKGDETDQILDVERSDSLVDGTEDTESITEHSDSASMQDSEYVNPQGVRFTPHHRHKDGSGPIIPYGLPCVRELMRFLISLINPYDRHNTDLMMHMGLNLLTIALETGCDHISHFSALLSLIKDDMCKNLLALLQADRLSLFAASLRVCFMLFESMRTHLKFQLEMFLHKLMEIIISESVRIPYEQREMALDSIVQLWRLPCMVTELYLNYDCDIYCSNLFEDLTKLLSKNAFPVSGSLFTTHLLSLEALLAVVDSIETNCHHRILSSMVAAQGHASPPDRDKAGESAVTIRVQDISGKEDGDKGKERGGKDEDGARQVGMGVTPPTTGYAMAQVMSANRDKEIDASKLKASEDFIEGSPNRRVSRFPPSANLPSLEQLMQIKKKKKMIQAGTEQFNLKPSKGIAFLIEQGILHTPLDPHEVAIFFKENPRLDKKMIGEYLSRKKNEKVLEAFLKLFVFEGTRIDEALRMYLEAFRLPGEAPVIQHLMEHFSAYWHNTNHQPYANSDAAFTLAYAVIMLNVDQHNHNAKKQNIPMNVEQFKKNLSKVNGGSDFDQSMLEEVFNAIKSEEIVMPAEQTGLVRDNYLWRVLLKRGASQEGTFLHAQCGELDREMFLLAWGPTVAALSFVFDKGMDDTITQKAIAGFRKCAMISAHYGLTDVFDNLVISLCKFTTLLSSMETPENLPIAFGSNAKAHLAAKTVFGLAHRHGDILAEGWKNLLDCMLQLYRAKLLPIEMVEVQDFVDPSGRVSLIREEMQPVKSDSSLLGSFYSYFTTAETSTQKGPTNEDKEAIEQARSCIEDCHPEHLITESKFLRQESLQELMKALTFASQGPQAADSLGMQYEEEAAVFNFELLIRVVLENRDRVGTLWQGICDHLYSLIVSQSEYSQLVERAVVGILRLAIRLLRKEDIAAQVLTSLRMLLLMKKSVLQRVCRQVAYGLHELLRTNAANIHSSQDWYTLFALLECVGAGAVPPSVHMVDGKRNLEISDTGTQSDSEMSTGSISGASDQGYTSDSELFKHRQPHRSSSEPDVSTMTDDRVIVVNNDSTVRHTHNHSKGVSKPQNQFEIALDQTLSHHDTKALMKCCESLAFLVRDAAHVTPANFESCVHAIRSFVEATVNGGKLRHDRQKKPEPTKDKKNSRMSRNQRNKKGMPKISSVPEKISQMADGADDGEDDGPPGGYHSLSIQLLDLMHTLHTRAASIFSSWAEEEKKGGSQMTIDAGASALWVKCWCPLLQGIARLCCDIRRQVRMQALTYLQRALLVHDLQTLSAVEWESCFNKVLFPLLAKLLDQIHPQDPVGLEETRMRAATLLCKVFLQHLTPLLSLSTFTALWLTILDFMDKYMHADKSDLLYEAIPESLKNMLLVMDTAGVFQDAAGSERGQNCQLWVFTWERIDCFLPGLKNEVFKPHEPVVSKEPAPSMAPTPDDIVRDGSPIEEEQVTVSAKPEEASPKPSTQDATPTKDALPTVGTAATPTTTPSTIETNPVTSQPNIILQPPLPYLAPLPGAQEAKPGSSVPFLLNPAILKGSPIPIVAPILPQPESSTTGSS

Target ORF information:

RefSeq Version	XM_022233960.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC110978671), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022233960.1

ORF Insert Sequence:

ATGGCAACCA TGGTAGTAGC TCCAAAGATA CAACCACCCA CTAATGGGGT CTACATTGTT 
CAAGGGGAGA TGTTGCTAGT TTCTACAGCC ATGAGGCGGA GCTCCAGATG GACATCCCAT 
TTGCCACAGG AAGAGGAACA GGACCCTTTG CTAGGAAGCT TCTCTCGTCT GAAGGAAATC 
TTGAACTCCA TCTCAGATAT AGCAGATATA GAGCCCAATG TGTTTTTATG TCCTTTCCTC 
GATGTCATCC GGTCTGAAGA CACTACTGGA CCAATCACAG GCTTCGCTCT GTCCTCCATC 
AACAAGTTTC TGTCCTATGG AATGCTAGAC ACATCAGTTG AAGGTGCCAC CTCAGGGATT 
GAAAACATAG CGGATGCCGT CACCCACGCA AGGTTTGTTG GTACAGATCC AGGATCCGAC 
GAAGTGGTAC TCATGAAGAT TCTCCAGGTT CTCAGGACTT TACTTCTGAC CCCTGTTGGA 
GCACACCTCT CCAATGAGTC TGTCTGTGAG ATCATGCAGT CTTGCTTCAG AATTTGCTTT 
GAGATGAGAC TAAGTGAGCT TCTACGCCGA TCGGCAGAGC ACACGCTGGT TGATATGGTG 
CAGCTGCTCT TCTCGAGATT GCCACAGTTC AAAGAGGAGA AACTCTCGGG CATGAGAAAG 
TTAAAAATGC GGCCAGGCGG CATAACTGAC GGAAGTCGCC GCCGCAAATC CCGTCCCTCT 
CCCAAAGTCC GTGCCAAGAA GGACCGTCTT GCCACCACAC CAACTGACGA GCCCTCCATG 
CAATCAGGGG GGGCCGCTTC TCCGCCGACA TCCAACACCA ACGCCTTGGG CCCGACAGTG 
ACTGCACCGG TTACAGCGAC ATCTCCAGGA GCAGCATCCG CTACCATGTC TGCTGTCACA 
ACCGTCACCC CCGGCACTGC GACCACTGCC TCCTCGGAAG CAAGCCAGTC TCAGGTCAAT 
CTGCCGTCAA CCCTGGACGT ACCCTCAGAG GCAGACGAGC AGGCCCTGCC GCCTTCGCCA 
TCCCTCGACA GTGGCGTGGA CATGAAGGAT TCGCTCTCAG CCGTGACCCC TTCAGAGAGT 
GACGTCACCC CAACCCAAGG TCAGGTCATC TCCATGCAAG GAGAGCAGCA GGAGGAGCCT 
ACAGAGAACA AGAAGGGTGA TGAGACTGAC CAGATTCTTG ATGTGGAACG ATCCGATTCC 
TTAGTGGATG GTACCGAAGA CACTGAGTCC ATCACAGAGC ATTCTGACAG TGCCTCCATG 
CAAGACTCTG AATACGTCAA TCCCCAGGGG GTCAGGTTCA CACCCCACCA CAGGCATAAA 
GATGGCTCGG GGCCCATTAT ACCCTATGGG CTACCTTGTG TCAGGGAGTT GATGAGATTC 
CTCATCTCCC TCATCAATCC ATATGACAGG CATAACACAG ACTTGATGAT GCACATGGGT 
CTGAACCTTT TGACCATCGC CTTGGAGACG GGATGCGATC ACATTTCTCA TTTTTCTGCT 
CTGCTCAGTC TCATCAAAGA TGACATGTGC AAGAACCTGT TGGCTCTCCT GCAGGCAGAC 
AGGCTGTCGC TCTTCGCAGC CTCCTTGCGT GTCTGCTTCA TGCTCTTTGA GTCCATGAGG 
ACCCATCTCA AGTTCCAGCT GGAGATGTTC CTGCACAAGC TGATGGAGAT CATCATCTCA 
GAGTCAGTCC GCATTCCCTA CGAGCAGCGT GAGATGGCAC TGGACTCCAT TGTGCAGCTG 
TGGCGACTCC CCTGTATGGT GACAGAACTC TATCTGAACT ATGACTGTGA CATCTACTGC 
AGTAATTTAT TTGAAGATCT GACCAAGTTG CTGTCCAAGA ATGCCTTCCC AGTCTCGGGG 
TCTCTTTTCA CCACCCACCT GCTCTCACTG GAGGCCCTGC TGGCTGTGGT AGACAGCATT 
GAGACTAACT GCCACCACCG CATCCTGAGC TCCATGGTGG CTGCCCAGGG CCATGCCAGC 
CCCCCGGACA GGGACAAGGC TGGTGAGTCG GCCGTCACCA TCCGTGTCCA GGACATCTCG 
GGAAAGGAGG ACGGTGACAA GGGGAAGGAG AGAGGAGGGA AGGATGAGGA TGGAGCCAGG 
CAGGTCGGAA TGGGCGTGAC TCCCCCCACC ACAGGCTACG CCATGGCTCA GGTCATGTCG 
GCGAACCGCG ACAAAGAGAT AGACGCAAGC AAGTTGAAGG CCTCAGAGGA CTTCATCGAG 
GGGAGCCCCA ACCGACGAGT CAGCCGCTTC CCTCCCTCAG CCAATCTGCC ATCGCTGGAG 
CAACTCATGC AGATCAAAAA AAAGAAAAAG ATGATCCAGG CGGGAACAGA ACAGTTCAAC 
CTCAAGCCCT CCAAGGGCAT CGCATTCCTG ATAGAACAGG GCATCCTACA CACCCCGCTG 
GACCCCCACG AGGTGGCCAT CTTCTTCAAA GAGAACCCCC GACTGGACAA GAAGATGATT 
GGGGAATACC TCAGCAGGAA GAAGAACGAG AAGGTCTTGG AAGCTTTTTT GAAGTTGTTT 
GTGTTTGAGG GCACCAGGAT TGATGAAGCC CTTAGGATGT ACCTGGAAGC CTTCCGACTG 
CCAGGAGAGG CTCCTGTCAT CCAACATCTC ATGGAGCACT TCTCAGCCTA CTGGCATAAC 
ACCAACCATC AGCCATATGC AAACAGTGAC GCTGCTTTTA CACTAGCCTA CGCAGTCATC 
ATGTTGAATG TGGACCAGCA CAACCATAAT GCCAAGAAGC AGAACATTCC AATGAACGTC 
GAGCAATTTA AGAAGAATCT GAGCAAGGTG AATGGCGGGT CAGATTTTGA TCAATCCATG 
CTGGAAGAAG TCTTCAATGC TATCAAGAGC GAGGAGATTG TGATGCCGGC TGAGCAGACG 
GGCTTAGTCC GTGACAACTA CTTGTGGCGT GTGCTGCTGA AGCGAGGGGC CAGTCAGGAG 
GGTACCTTCC TGCATGCCCA GTGCGGTGAG CTGGACCGTG AGATGTTCCT GCTGGCCTGG 
GGACCCACAG TGGCTGCCCT GTCCTTTGTC TTTGACAAAG GCATGGATGA CACCATCACA 
CAGAAGGCTA TCGCTGGATT CAGGAAATGT GCAATGATCT CAGCCCATTA CGGTTTGACG 
GACGTGTTCG ACAATTTGGT CATCTCCCTC TGCAAGTTCA CCACTCTTCT GAGTTCCATG 
GAGACCCCAG AGAACCTTCC AATCGCCTTT GGCTCCAATG CCAAGGCCCA CCTTGCGGCC 
AAGACAGTCT TTGGCTTGGC CCATCGCCAT GGCGACATCC TAGCCGAAGG CTGGAAGAAC 
CTCCTGGACT GCATGCTGCA GCTGTATCGG GCCAAGCTCC TCCCCATCGA GATGGTAGAG 
GTCCAAGACT TCGTGGATCC TAGCGGCAGG GTCTCCCTCA TAAGAGAGGA GATGCAACCA 
GTCAAGTCTG ATTCGTCCTT ACTTGGCTCA TTCTACTCCT ACTTCACAAC TGCTGAGACA 
TCTACTCAGA AAGGCCCTAC CAATGAAGAT AAAGAGGCCA TTGAGCAAGC CAGGAGCTGT 
ATAGAGGATT GTCACCCAGA GCACCTGATC ACGGAGAGCA AGTTCCTGCG TCAGGAGTCA 
CTGCAGGAGC TGATGAAGGC CCTGACCTTT GCCTCCCAGG GCCCTCAGGC AGCCGACTCA 
CTGGGGATGC AGTACGAGGA GGAGGCGGCA GTCTTTAACT TTGAGCTCCT CATCAGGGTT 
GTCTTGGAAA ACAGGGATCG GGTCGGTACC CTGTGGCAGG GCATCTGTGA TCATCTCTAC 
AGTCTGATTG TATCGCAATC AGAGTACAGC CAGCTGGTTG AGAGGGCCGT GGTGGGCATC 
CTACGACTTG CTATACGCCT GCTGAGGAAG GAAGACATTG CTGCTCAGGT CTTGACATCT 
CTCCGAATGC TGCTGCTGAT GAAGAAGTCG GTCCTGCAGC GCGTCTGTCG CCAGGTGGCC 
TATGGCCTTC ACGAGCTGCT ACGCACCAAC GCTGCCAACA TCCACTCCTC CCAGGACTGG 
TATACGCTCT TTGCTCTGCT GGAGTGCGTT GGGGCTGGGG CAGTGCCCCC TAGCGTCCAC 
ATGGTAGACG GCAAGAGGAA TCTGGAGATA TCAGACACAG GCACCCAGTC AGATAGCGAG 
ATGTCAACCG GCAGTATTAG TGGTGCCTCA GACCAAGGCT ACACGTCTGA CAGCGAACTC 
TTCAAACACA GGCAGCCTCA CCGATCGAGC AGTGAGCCCG ACGTGTCAAC CATGACAGAT 
GACAGAGTCA TTGTGGTGAA CAATGACAGC ACAGTCCGCC ACACCCACAA CCATTCCAAA 
GGCGTTTCCA AGCCCCAGAA CCAGTTTGAG ATAGCCTTGG ATCAGACGCT GAGCCACCAT 
GACACCAAGG CCCTCATGAA GTGCTGCGAG TCATTGGCAT TCCTGGTGCG AGACGCCGCC 
CACGTCACAC CCGCCAACTT TGAGTCTTGC GTCCATGCCA TCAGGAGCTT TGTGGAGGCC 
ACAGTCAATG GGGGGAAACT TAGACACGAT CGACAAAAGA AACCTGAGCC GACAAAAGAC 
AAGAAAAACT CCCGAATGTC ACGGAATCAG AGGAATAAAA AGGGTATGCC CAAGATAAGC 
TCTGTACCCG AAAAGATCTC GCAGATGGCA GACGGGGCAG ACGATGGGGA AGATGATGGA 
CCTCCTGGAG GCTATCACTC ATTGTCTATA CAGTTACTGG ACCTGATGCA CACCCTCCAC 
ACGAGGGCAG CATCCATCTT CAGTTCCTGG GCTGAGGAAG AGAAGAAAGG TGGATCACAG 
ATGACGATTG ATGCAGGAGC AAGTGCATTG TGGGTCAAAT GCTGGTGCCC GCTACTTCAA 
GGCATCGCCC GGCTGTGCTG CGACATACGA CGCCAGGTAC GCATGCAGGC CCTGACCTAC 
CTGCAGCGGG CTCTGCTTGT GCATGACCTG CAGACGCTGT CGGCTGTAGA GTGGGAATCC 
TGCTTCAACA AGGTCCTGTT CCCGTTGCTC GCCAAGCTGC TGGACCAGAT CCACCCTCAG 
GACCCAGTGG GGCTGGAGGA GACACGCATG AGGGCTGCCA CCCTGCTGTG CAAGGTATTC 
TTACAGCATT TGACACCCCT TCTATCGCTG TCTACATTCA CGGCACTGTG GCTCACCATC 
CTGGATTTCA TGGACAAGTA CATGCATGCT GACAAGAGTG ACCTACTTTA TGAGGCCATC 
CCGGAATCCT TGAAGAACAT GCTTTTGGTC ATGGATACAG CAGGCGTCTT TCAAGATGCG 
GCCGGCTCAG AGCGAGGCCA GAACTGCCAG CTCTGGGTCT TCACCTGGGA GCGTATTGAC 
TGCTTCTTGC CAGGCCTCAA GAATGAAGTC TTCAAGCCCC ACGAGCCAGT TGTGAGCAAA 
GAGCCGGCAC CAAGCATGGC CCCTACACCA GATGACATCG TTAGAGATGG CAGTCCAATT 
GAAGAGGAGC AAGTTACTGT CAGTGCAAAA CCAGAAGAAG CAAGCCCAAA ACCTTCAACA 
CAGGATGCCA CCCCCACAAA AGATGCCCTG CCCACTGTAG GAACAGCAGC CACGCCTACA 
ACCACACCCA GCACCATTGA GACCAACCCA GTCACCAGCC AGCCCAACAT CATCCTGCAG 
CCGCCCCTGC CCTACCTGGC CCCCCTTCCT GGGGCCCAAG AGGCCAAGCC GGGCAGTTCT 
GTGCCGTTCC TCCTTAATCC GGCCATCCTG AAAGGCTCGC CTATCCCCAT CGTCGCGCCC 
ATACTCCCGC AGCCCGAATC CTCCACAACG GGAAGCTCTT AG