ANKRD28 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD28
Entrez Gene ID	109561811
Full Name	ankyrin repeat domain 28
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019964561.1	XP_019820120.1	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X2
XM_019964553.1	XP_019820112.1	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X1

Pathways from BioSystems
Gene Ontology

The following ANKRD28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo26930	XM_019964553.1 Latest version!	Bos indicus ankyrin repeat domain 28 (ANKRD28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo26931	XM_019964561.1 Latest version!	Bos indicus ankyrin repeat domain 28 (ANKRD28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD28 ( XM_019964553.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019820112.1 Bos indicus ankyrin repeat domain 28 (ANKRD28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo26930
Clone ID Related Accession (Same CDS sequence)	XM_019964553.1
Accession Version	XM_019964553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032650.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAG TGTGTATTGT TGTTTTGGAG GAGGTAGAAG ATGAGTCTCC TGAATTCATT 
TGTAAATTGC CACAGGAAAA TAAATCCCTA CATTCTGCAC CTTCGGGAAA TGTATTGCCC 
TCACTGGTGC AAGCTATATT TAATGGAGAT CCTGATGAAG TTCGAGCACT AATATTTAAG 
AAAGAAGATG TTAACTTTCA GGACAATGAA AAGAGAACTC CACTGCATGC TGCTGCCTAT 
CTTGGAGATG CAGAAATCAT TGAATTGCTT ATTTTATCTG GAGCTAGAGT TAATGCCAAA 
GACAGCAAAT GGTTGACACC TTTACACAGG GCAGTTGCAT CTTGTAGTGA GGAAGCAGTT 
CAGGTGCTGT TGAAGCATTC CGCAGATGTT AACGCTCGAG ACAAAAACTG GCAAACCCCT 
TTGCACACAG CGGCCGCTAA CAAAGCCGTT AAGTGCGCGG AGGCCTTGGT GCCTCTTCTG 
AGCAATGTGA ACGTGTCTGA CCGGGCAGGG AGGACTGCGC TGCACCATGC AGCTCTCAGC 
GGCCATGGGG AGATGGTCAA ATTACTCTTG TCTAGAGGTG CCAATATTAA CGCTTTTGAC 
AAGAAAGATA GACGTGCTAT CCACTGGGCA GCATATATGG GTCACATTGA AGTAGTGAAA 
TTACTTGTGG CACACGGAGC TGAGGTGACA TGCAAGGACA AGAAGTCTTA CACGCCTCTC 
CACGCAGCAG CCTCCAGTGG GATGATCAGC GTAGTCAAGT ACCTTCTCGA TCTTGGAGTT 
GATATGAATG AGCCAAATGC CTATGGAAAT ACACCTCTTC ACGTAGCCTG CTACAATGGA 
CAGGATGTTG TCGTGAATGA ACTGATAGAC TCTGGTGCTA ATGTAAATCA GAAGAATGAG 
AAAGGGTTTA CTCCTTTGCA CTTTGCTGCT GCATCTACAC ACGGAGCATT GTGTTTAGAG 
CTTCTAGTAG GCAATGGGGC CGACGTCAAT ATGAAGAGCA AAGATGGAAA AACTCCACTG 
CACATGACTG CCCTCCATGG TAGATTCTCC AGATCACAAA CCATTATCCA GAGTGGAGCT 
GTAATCGACT GTGAAGATAA GAATGGCAAC ACTCCTTTGC ACATAGCAGC GAGGTATGGC 
CACGAGCTGC TCATCAACAC CCTCATCACG AGCGGGGCCG ACACCGCCAA ACGTGGCATA 
CATGGAATGT TCCCCCTCCA TTTGGCAGCC TTAAGTGGTT TTTCAGATTG CTGTAGGAAA 
CTTCTTTCTT CTGGATTTGA TATAGATACC CCAGATGATT TTGGCAGGAC CTGTCTACAT 
GCAGCTGCAG CTGGAGGTAA TTTGGAGTGC CTAAACCTTC TGCTGAATAC TGGTGCAGAC 
TTCAACAAAA AAGACAAGTT TGGAAGATCT CCACTGCATT ACGCTGCTGC CAACTGCAAC 
TACCAGTGCC TGTTTGCTCT TGTGGGCTCA GGAGCAAGCG TGAATGACCT TGATGAGCGA 
GGATGCACGC CCCTGCACTA CGCAGCGACG TCAGACACGG ACGGCAAGTG CCTGGAATAC 
TTATTAAGAA ATGATGCAAA TCCAGGGATC CGGGACAAGC AAGGATACAA TGCCGTTCAT 
TATTCAGCTG CATATGGTCA CCGTCTGTGT CTTCAGCTGA TTGCAAGTGA AACTCCTTTA 
GATGTTTTAA TGGAAACATC GGGAACAGAC ATGCTGAATG ATTCAGACAA TAGAGCAACA 
ATAAGCCCTT TACACTTGGC TGCCTATCAT GGTCACCATC AAGCCCTGGA AGTGTTAGTC 
CAGTCTCTGT TAGATCTTGA CGTGAGAAAC AGTAGTGGAA GAACCCCCTT GGACCTTGCA 
GCATTTAAGG GCCATGTAGA ATGTGTGGAT GTTCTCATTA ATCAGGGAGC CTCAATCTTA 
GTCAAAGATT ATATTTTGAA GAGAACACCT ATACATGCAG CAGCAACAAA TGGTCATTCG 
GAATGCTTAC GGCTACTAAT AGGAAATGCC GAACCACAGA ATGCAGTAGA TATTCAAGAT 
GGAAATGGAC AGACTCCTCT GATGCTGTCT GTGCTCAACG GGCACACAGA CTGTGTGTAC 
TCACTGCTCA ACAAAGGCGC GAACGTCGAT GCCAAAGACA GGTGGGGAAG GACGGCGCTG 
CACAGAGGGG CAGTCACAGG CCATGAAGAG TGTGTGGATG CATTACTTCA ACATGGTGCT 
AACTGCTTAT TTCGAGATAG CAGGGGCCGG ACACCAATAC ACTTGTCAGC TGCCTGTGGA 
CACATTGGTG TTCTTGGAGC CCTTCTGCAG TCAGCAGCAT CTATGGATGC AAACCCAGCC 
ATGGTGGACA ATCACGGATA CACAGCACTT CACTGGGCCT GCTACAATGG TCATGAGACA 
TGTGTAGAAC TCCTTTTAGA ACAAGACGTT TTCCAGAAAA CAGAAGGAAA TGCTTTCAGT 
CCGTTGCATT GTGCTGTAAT AAATGACAAC GAAGGTGCTG CTGAGATGTT AATTGATACA 
TTAGGCTCCA GCATTGTGAA CGCCACAGAT TCAAAAGGAA GAACTCCTCT GCACGCAGCC 
GCCTTCACAG ACCATGTGGA GTGTCTGCAG CTTCTGCTCA GCCATAATGC TCACGTGAAC 
TCTGTGGACT CCTCTGGGAA AACACCTCTC ATGATGGCTG CAGAAAACGG ACAGACAAAC 
ACAGTTGAGA TGCTGGTTAG CAGTGCTAGT GCAGATCTGA CTTTACAAGA TAACAGTAAA 
AACACCGCCC TCCATTTGGC TTGCAGTAAG GGTCATGAAA CTAGTGCCTT GTTAATACTG 
GAAAAGATAA CAGATAGAAA CCTCATCAAT GCAACCAATG CAGCTTTGCA AACACCTCTG 
CATGTTGCTG CCCGAAACGG GCTGACAATG GTGGTTCAGG AACTCCTGGG GAAAGGGGCA 
AGCGTGCTTG CGGTCGATGA GAACGGCTAT ACCCCAGCTT TGGCCTGTGC TCCCAACAAG 
GACGTTGCAG ACTGCCTCGC ACTCATTCTG GCCACCATGA TGCCTATCTC GTCAAGTAGC 
CCTTTATCAT CCCTGACGTT CAATGCCATT AACCGTTACA CCAACACCTC GAAAACTGTC 
AGCTTCGAAG CCTTGCCCAT CATGAGAAAT GAACCCAGCT CCTACTGTAG CTTCAACAAC 
ATCGGCGGGG AGCAGGAGTA CCTATACACT GATGTGGACG AGCTCAATGA CTCCGATTCA 
GAGACCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019820112.1
CDS	1421..4672
Translation	MSRVCIVVLEEVEDESPEFICKLPQENKSLHSAPSGNVLPSLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHTAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAALSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVAHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDSGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTIIQSGAVIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDKFGRSPLHYAAANCNYQCLFALVGSGASVNDLDERGCTPLHYAATSDTDGKCLEYLLRNDANPGIRDKQGYNAVHYSAAYGHRLCLQLIASETPLDVLMETSGTDMLNDSDNRATISPLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDRWGRTALHRGAVTGHEECVDALLQHGANCLFRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPAMVDNHGYTALHWACYNGHETCVELLLEQDVFQKTEGNAFSPLHCAVINDNEGAAEMLIDTLGSSIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAHVNSVDSSGKTPLMMAAENGQTNTVEMLVSSASADLTLQDNSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLGKGASVLAVDENGYTPALACAPNKDVADCLALILATMMPISSSSPLSSLTFNAINRYTNTSKTVSFEALPIMRNEPSSYCSFNNIGGEQEYLYTDVDELNDSDSETY

Target ORF information:

RefSeq Version	XM_019964553.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus ankyrin repeat domain 28 (ANKRD28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019964553.1

ORF Insert Sequence:

ATGAGTCGAG TGTGTATTGT TGTTTTGGAG GAGGTAGAAG ATGAGTCTCC TGAATTCATT 
TGTAAATTGC CACAGGAAAA TAAATCCCTA CATTCTGCAC CTTCGGGAAA TGTATTGCCC 
TCACTGGTGC AAGCTATATT TAATGGAGAT CCTGATGAAG TTCGAGCACT AATATTTAAG 
AAAGAAGATG TTAACTTTCA GGACAATGAA AAGAGAACTC CACTGCATGC TGCTGCCTAT 
CTTGGAGATG CAGAAATCAT TGAATTGCTT ATTTTATCTG GAGCTAGAGT TAATGCCAAA 
GACAGCAAAT GGTTGACACC TTTACACAGG GCAGTTGCAT CTTGTAGTGA GGAAGCAGTT 
CAGGTGCTGT TGAAGCATTC CGCAGATGTT AACGCTCGAG ACAAAAACTG GCAAACCCCT 
TTGCACACAG CGGCCGCTAA CAAAGCCGTT AAGTGCGCGG AGGCCTTGGT GCCTCTTCTG 
AGCAATGTGA ACGTGTCTGA CCGGGCAGGG AGGACTGCGC TGCACCATGC AGCTCTCAGC 
GGCCATGGGG AGATGGTCAA ATTACTCTTG TCTAGAGGTG CCAATATTAA CGCTTTTGAC 
AAGAAAGATA GACGTGCTAT CCACTGGGCA GCATATATGG GTCACATTGA AGTAGTGAAA 
TTACTTGTGG CACACGGAGC TGAGGTGACA TGCAAGGACA AGAAGTCTTA CACGCCTCTC 
CACGCAGCAG CCTCCAGTGG GATGATCAGC GTAGTCAAGT ACCTTCTCGA TCTTGGAGTT 
GATATGAATG AGCCAAATGC CTATGGAAAT ACACCTCTTC ACGTAGCCTG CTACAATGGA 
CAGGATGTTG TCGTGAATGA ACTGATAGAC TCTGGTGCTA ATGTAAATCA GAAGAATGAG 
AAAGGGTTTA CTCCTTTGCA CTTTGCTGCT GCATCTACAC ACGGAGCATT GTGTTTAGAG 
CTTCTAGTAG GCAATGGGGC CGACGTCAAT ATGAAGAGCA AAGATGGAAA AACTCCACTG 
CACATGACTG CCCTCCATGG TAGATTCTCC AGATCACAAA CCATTATCCA GAGTGGAGCT 
GTAATCGACT GTGAAGATAA GAATGGCAAC ACTCCTTTGC ACATAGCAGC GAGGTATGGC 
CACGAGCTGC TCATCAACAC CCTCATCACG AGCGGGGCCG ACACCGCCAA ACGTGGCATA 
CATGGAATGT TCCCCCTCCA TTTGGCAGCC TTAAGTGGTT TTTCAGATTG CTGTAGGAAA 
CTTCTTTCTT CTGGATTTGA TATAGATACC CCAGATGATT TTGGCAGGAC CTGTCTACAT 
GCAGCTGCAG CTGGAGGTAA TTTGGAGTGC CTAAACCTTC TGCTGAATAC TGGTGCAGAC 
TTCAACAAAA AAGACAAGTT TGGAAGATCT CCACTGCATT ACGCTGCTGC CAACTGCAAC 
TACCAGTGCC TGTTTGCTCT TGTGGGCTCA GGAGCAAGCG TGAATGACCT TGATGAGCGA 
GGATGCACGC CCCTGCACTA CGCAGCGACG TCAGACACGG ACGGCAAGTG CCTGGAATAC 
TTATTAAGAA ATGATGCAAA TCCAGGGATC CGGGACAAGC AAGGATACAA TGCCGTTCAT 
TATTCAGCTG CATATGGTCA CCGTCTGTGT CTTCAGCTGA TTGCAAGTGA AACTCCTTTA 
GATGTTTTAA TGGAAACATC GGGAACAGAC ATGCTGAATG ATTCAGACAA TAGAGCAACA 
ATAAGCCCTT TACACTTGGC TGCCTATCAT GGTCACCATC AAGCCCTGGA AGTGTTAGTC 
CAGTCTCTGT TAGATCTTGA CGTGAGAAAC AGTAGTGGAA GAACCCCCTT GGACCTTGCA 
GCATTTAAGG GCCATGTAGA ATGTGTGGAT GTTCTCATTA ATCAGGGAGC CTCAATCTTA 
GTCAAAGATT ATATTTTGAA GAGAACACCT ATACATGCAG CAGCAACAAA TGGTCATTCG 
GAATGCTTAC GGCTACTAAT AGGAAATGCC GAACCACAGA ATGCAGTAGA TATTCAAGAT 
GGAAATGGAC AGACTCCTCT GATGCTGTCT GTGCTCAACG GGCACACAGA CTGTGTGTAC 
TCACTGCTCA ACAAAGGCGC GAACGTCGAT GCCAAAGACA GGTGGGGAAG GACGGCGCTG 
CACAGAGGGG CAGTCACAGG CCATGAAGAG TGTGTGGATG CATTACTTCA ACATGGTGCT 
AACTGCTTAT TTCGAGATAG CAGGGGCCGG ACACCAATAC ACTTGTCAGC TGCCTGTGGA 
CACATTGGTG TTCTTGGAGC CCTTCTGCAG TCAGCAGCAT CTATGGATGC AAACCCAGCC 
ATGGTGGACA ATCACGGATA CACAGCACTT CACTGGGCCT GCTACAATGG TCATGAGACA 
TGTGTAGAAC TCCTTTTAGA ACAAGACGTT TTCCAGAAAA CAGAAGGAAA TGCTTTCAGT 
CCGTTGCATT GTGCTGTAAT AAATGACAAC GAAGGTGCTG CTGAGATGTT AATTGATACA 
TTAGGCTCCA GCATTGTGAA CGCCACAGAT TCAAAAGGAA GAACTCCTCT GCACGCAGCC 
GCCTTCACAG ACCATGTGGA GTGTCTGCAG CTTCTGCTCA GCCATAATGC TCACGTGAAC 
TCTGTGGACT CCTCTGGGAA AACACCTCTC ATGATGGCTG CAGAAAACGG ACAGACAAAC 
ACAGTTGAGA TGCTGGTTAG CAGTGCTAGT GCAGATCTGA CTTTACAAGA TAACAGTAAA 
AACACCGCCC TCCATTTGGC TTGCAGTAAG GGTCATGAAA CTAGTGCCTT GTTAATACTG 
GAAAAGATAA CAGATAGAAA CCTCATCAAT GCAACCAATG CAGCTTTGCA AACACCTCTG 
CATGTTGCTG CCCGAAACGG GCTGACAATG GTGGTTCAGG AACTCCTGGG GAAAGGGGCA 
AGCGTGCTTG CGGTCGATGA GAACGGCTAT ACCCCAGCTT TGGCCTGTGC TCCCAACAAG 
GACGTTGCAG ACTGCCTCGC ACTCATTCTG GCCACCATGA TGCCTATCTC GTCAAGTAGC 
CCTTTATCAT CCCTGACGTT CAATGCCATT AACCGTTACA CCAACACCTC GAAAACTGTC 
AGCTTCGAAG CCTTGCCCAT CATGAGAAAT GAACCCAGCT CCTACTGTAG CTTCAACAAC 
ATCGGCGGGG AGCAGGAGTA CCTATACACT GATGTGGACG AGCTCAATGA CTCCGATTCA 
GAGACCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD28 ( XM_019964561.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019820120.1 Bos indicus ankyrin repeat domain 28 (ANKRD28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo26931
Clone ID Related Accession (Same CDS sequence)	XM_019964561.1
Accession Version	XM_019964561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032650.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTAA GGGACAATGA AAAGAGAACT CCACTGCATG CTGCTGCCTA TCTTGGAGAT 
GCAGAAATCA TTGAATTGCT TATTTTATCT GGAGCTAGAG TTAATGCCAA AGACAGCAAA 
TGGTTGACAC CTTTACACAG GGCAGTTGCA TCTTGTAGTG AGGAAGCAGT TCAGGTGCTG 
TTGAAGCATT CCGCAGATGT TAACGCTCGA GACAAAAACT GGCAAACCCC TTTGCACACA 
GCGGCCGCTA ACAAAGCCGT TAAGTGCGCG GAGGCCTTGG TGCCTCTTCT GAGCAATGTG 
AACGTGTCTG ACCGGGCAGG GAGGACTGCG CTGCACCATG CAGCTCTCAG CGGCCATGGG 
GAGATGGTCA AATTACTCTT GTCTAGAGGT GCCAATATTA ACGCTTTTGA CAAGAAAGAT 
AGACGTGCTA TCCACTGGGC AGCATATATG GGTCACATTG AAGTAGTGAA ATTACTTGTG 
GCACACGGAG CTGAGGTGAC ATGCAAGGAC AAGAAGTCTT ACACGCCTCT CCACGCAGCA 
GCCTCCAGTG GGATGATCAG CGTAGTCAAG TACCTTCTCG ATCTTGGAGT TGATATGAAT 
GAGCCAAATG CCTATGGAAA TACACCTCTT CACGTAGCCT GCTACAATGG ACAGGATGTT 
GTCGTGAATG AACTGATAGA CTCTGGTGCT AATGTAAATC AGAAGAATGA GAAAGGGTTT 
ACTCCTTTGC ACTTTGCTGC TGCATCTACA CACGGAGCAT TGTGTTTAGA GCTTCTAGTA 
GGCAATGGGG CCGACGTCAA TATGAAGAGC AAAGATGGAA AAACTCCACT GCACATGACT 
GCCCTCCATG GTAGATTCTC CAGATCACAA ACCATTATCC AGAGTGGAGC TGTAATCGAC 
TGTGAAGATA AGAATGGCAA CACTCCTTTG CACATAGCAG CGAGGTATGG CCACGAGCTG 
CTCATCAACA CCCTCATCAC GAGCGGGGCC GACACCGCCA AACGTGGCAT ACATGGAATG 
TTCCCCCTCC ATTTGGCAGC CTTAAGTGGT TTTTCAGATT GCTGTAGGAA ACTTCTTTCT 
TCTGGATTTG ATATAGATAC CCCAGATGAT TTTGGCAGGA CCTGTCTACA TGCAGCTGCA 
GCTGGAGGTA ATTTGGAGTG CCTAAACCTT CTGCTGAATA CTGGTGCAGA CTTCAACAAA 
AAAGACAAGT TTGGAAGATC TCCACTGCAT TACGCTGCTG CCAACTGCAA CTACCAGTGC 
CTGTTTGCTC TTGTGGGCTC AGGAGCAAGC GTGAATGACC TTGATGAGCG AGGATGCACG 
CCCCTGCACT ACGCAGCGAC GTCAGACACG GACGGCAAGT GCCTGGAATA CTTATTAAGA 
AATGATGCAA ATCCAGGGAT CCGGGACAAG CAAGGATACA ATGCCGTTCA TTATTCAGCT 
GCATATGGTC ACCGTCTGTG TCTTCAGCTG ATTGCAAGTG AAACTCCTTT AGATGTTTTA 
ATGGAAACAT CGGGAACAGA CATGCTGAAT GATTCAGACA ATAGAGCAAC AATAAGCCCT 
TTACACTTGG CTGCCTATCA TGGTCACCAT CAAGCCCTGG AAGTGTTAGT CCAGTCTCTG 
TTAGATCTTG ACGTGAGAAA CAGTAGTGGA AGAACCCCCT TGGACCTTGC AGCATTTAAG 
GGCCATGTAG AATGTGTGGA TGTTCTCATT AATCAGGGAG CCTCAATCTT AGTCAAAGAT 
TATATTTTGA AGAGAACACC TATACATGCA GCAGCAACAA ATGGTCATTC GGAATGCTTA 
CGGCTACTAA TAGGAAATGC CGAACCACAG AATGCAGTAG ATATTCAAGA TGGAAATGGA 
CAGACTCCTC TGATGCTGTC TGTGCTCAAC GGGCACACAG ACTGTGTGTA CTCACTGCTC 
AACAAAGGCG CGAACGTCGA TGCCAAAGAC AGGTGGGGAA GGACGGCGCT GCACAGAGGG 
GCAGTCACAG GCCATGAAGA GTGTGTGGAT GCATTACTTC AACATGGTGC TAACTGCTTA 
TTTCGAGATA GCAGGGGCCG GACACCAATA CACTTGTCAG CTGCCTGTGG ACACATTGGT 
GTTCTTGGAG CCCTTCTGCA GTCAGCAGCA TCTATGGATG CAAACCCAGC CATGGTGGAC 
AATCACGGAT ACACAGCACT TCACTGGGCC TGCTACAATG GTCATGAGAC ATGTGTAGAA 
CTCCTTTTAG AACAAGACGT TTTCCAGAAA ACAGAAGGAA ATGCTTTCAG TCCGTTGCAT 
TGTGCTGTAA TAAATGACAA CGAAGGTGCT GCTGAGATGT TAATTGATAC ATTAGGCTCC 
AGCATTGTGA ACGCCACAGA TTCAAAAGGA AGAACTCCTC TGCACGCAGC CGCCTTCACA 
GACCATGTGG AGTGTCTGCA GCTTCTGCTC AGCCATAATG CTCACGTGAA CTCTGTGGAC 
TCCTCTGGGA AAACACCTCT CATGATGGCT GCAGAAAACG GACAGACAAA CACAGTTGAG 
ATGCTGGTTA GCAGTGCTAG TGCAGATCTG ACTTTACAAG ATAACAGTAA AAACACCGCC 
CTCCATTTGG CTTGCAGTAA GGGTCATGAA ACTAGTGCCT TGTTAATACT GGAAAAGATA 
ACAGATAGAA ACCTCATCAA TGCAACCAAT GCAGCTTTGC AAACACCTCT GCATGTTGCT 
GCCCGAAACG GGCTGACAAT GGTGGTTCAG GAACTCCTGG GGAAAGGGGC AAGCGTGCTT 
GCGGTCGATG AGAACGGCTA TACCCCAGCT TTGGCCTGTG CTCCCAACAA GGACGTTGCA 
GACTGCCTCG CACTCATTCT GGCCACCATG ATGCCTATCT CGTCAAGTAG CCCTTTATCA 
TCCCTGACGT TCAATGCCAT TAACCGTTAC ACCAACACCT CGAAAACTGT CAGCTTCGAA 
GCCTTGCCCA TCATGAGAAA TGAACCCAGC TCCTACTGTA GCTTCAACAA CATCGGCGGG 
GAGCAGGAGT ACCTATACAC TGATGTGGAC GAGCTCAATG ACTCCGATTC AGAGACCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019820120.1
CDS	92..3154
Translation	MGVRDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHTAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAALSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVAHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDSGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTIIQSGAVIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDKFGRSPLHYAAANCNYQCLFALVGSGASVNDLDERGCTPLHYAATSDTDGKCLEYLLRNDANPGIRDKQGYNAVHYSAAYGHRLCLQLIASETPLDVLMETSGTDMLNDSDNRATISPLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDRWGRTALHRGAVTGHEECVDALLQHGANCLFRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPAMVDNHGYTALHWACYNGHETCVELLLEQDVFQKTEGNAFSPLHCAVINDNEGAAEMLIDTLGSSIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAHVNSVDSSGKTPLMMAAENGQTNTVEMLVSSASADLTLQDNSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLGKGASVLAVDENGYTPALACAPNKDVADCLALILATMMPISSSSPLSSLTFNAINRYTNTSKTVSFEALPIMRNEPSSYCSFNNIGGEQEYLYTDVDELNDSDSETY

Target ORF information:

RefSeq Version	XM_019964561.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus ankyrin repeat domain 28 (ANKRD28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019964561.1

ORF Insert Sequence:

ATGGGTGTAA GGGACAATGA AAAGAGAACT CCACTGCATG CTGCTGCCTA TCTTGGAGAT 
GCAGAAATCA TTGAATTGCT TATTTTATCT GGAGCTAGAG TTAATGCCAA AGACAGCAAA 
TGGTTGACAC CTTTACACAG GGCAGTTGCA TCTTGTAGTG AGGAAGCAGT TCAGGTGCTG 
TTGAAGCATT CCGCAGATGT TAACGCTCGA GACAAAAACT GGCAAACCCC TTTGCACACA 
GCGGCCGCTA ACAAAGCCGT TAAGTGCGCG GAGGCCTTGG TGCCTCTTCT GAGCAATGTG 
AACGTGTCTG ACCGGGCAGG GAGGACTGCG CTGCACCATG CAGCTCTCAG CGGCCATGGG 
GAGATGGTCA AATTACTCTT GTCTAGAGGT GCCAATATTA ACGCTTTTGA CAAGAAAGAT 
AGACGTGCTA TCCACTGGGC AGCATATATG GGTCACATTG AAGTAGTGAA ATTACTTGTG 
GCACACGGAG CTGAGGTGAC ATGCAAGGAC AAGAAGTCTT ACACGCCTCT CCACGCAGCA 
GCCTCCAGTG GGATGATCAG CGTAGTCAAG TACCTTCTCG ATCTTGGAGT TGATATGAAT 
GAGCCAAATG CCTATGGAAA TACACCTCTT CACGTAGCCT GCTACAATGG ACAGGATGTT 
GTCGTGAATG AACTGATAGA CTCTGGTGCT AATGTAAATC AGAAGAATGA GAAAGGGTTT 
ACTCCTTTGC ACTTTGCTGC TGCATCTACA CACGGAGCAT TGTGTTTAGA GCTTCTAGTA 
GGCAATGGGG CCGACGTCAA TATGAAGAGC AAAGATGGAA AAACTCCACT GCACATGACT 
GCCCTCCATG GTAGATTCTC CAGATCACAA ACCATTATCC AGAGTGGAGC TGTAATCGAC 
TGTGAAGATA AGAATGGCAA CACTCCTTTG CACATAGCAG CGAGGTATGG CCACGAGCTG 
CTCATCAACA CCCTCATCAC GAGCGGGGCC GACACCGCCA AACGTGGCAT ACATGGAATG 
TTCCCCCTCC ATTTGGCAGC CTTAAGTGGT TTTTCAGATT GCTGTAGGAA ACTTCTTTCT 
TCTGGATTTG ATATAGATAC CCCAGATGAT TTTGGCAGGA CCTGTCTACA TGCAGCTGCA 
GCTGGAGGTA ATTTGGAGTG CCTAAACCTT CTGCTGAATA CTGGTGCAGA CTTCAACAAA 
AAAGACAAGT TTGGAAGATC TCCACTGCAT TACGCTGCTG CCAACTGCAA CTACCAGTGC 
CTGTTTGCTC TTGTGGGCTC AGGAGCAAGC GTGAATGACC TTGATGAGCG AGGATGCACG 
CCCCTGCACT ACGCAGCGAC GTCAGACACG GACGGCAAGT GCCTGGAATA CTTATTAAGA 
AATGATGCAA ATCCAGGGAT CCGGGACAAG CAAGGATACA ATGCCGTTCA TTATTCAGCT 
GCATATGGTC ACCGTCTGTG TCTTCAGCTG ATTGCAAGTG AAACTCCTTT AGATGTTTTA 
ATGGAAACAT CGGGAACAGA CATGCTGAAT GATTCAGACA ATAGAGCAAC AATAAGCCCT 
TTACACTTGG CTGCCTATCA TGGTCACCAT CAAGCCCTGG AAGTGTTAGT CCAGTCTCTG 
TTAGATCTTG ACGTGAGAAA CAGTAGTGGA AGAACCCCCT TGGACCTTGC AGCATTTAAG 
GGCCATGTAG AATGTGTGGA TGTTCTCATT AATCAGGGAG CCTCAATCTT AGTCAAAGAT 
TATATTTTGA AGAGAACACC TATACATGCA GCAGCAACAA ATGGTCATTC GGAATGCTTA 
CGGCTACTAA TAGGAAATGC CGAACCACAG AATGCAGTAG ATATTCAAGA TGGAAATGGA 
CAGACTCCTC TGATGCTGTC TGTGCTCAAC GGGCACACAG ACTGTGTGTA CTCACTGCTC 
AACAAAGGCG CGAACGTCGA TGCCAAAGAC AGGTGGGGAA GGACGGCGCT GCACAGAGGG 
GCAGTCACAG GCCATGAAGA GTGTGTGGAT GCATTACTTC AACATGGTGC TAACTGCTTA 
TTTCGAGATA GCAGGGGCCG GACACCAATA CACTTGTCAG CTGCCTGTGG ACACATTGGT 
GTTCTTGGAG CCCTTCTGCA GTCAGCAGCA TCTATGGATG CAAACCCAGC CATGGTGGAC 
AATCACGGAT ACACAGCACT TCACTGGGCC TGCTACAATG GTCATGAGAC ATGTGTAGAA 
CTCCTTTTAG AACAAGACGT TTTCCAGAAA ACAGAAGGAA ATGCTTTCAG TCCGTTGCAT 
TGTGCTGTAA TAAATGACAA CGAAGGTGCT GCTGAGATGT TAATTGATAC ATTAGGCTCC 
AGCATTGTGA ACGCCACAGA TTCAAAAGGA AGAACTCCTC TGCACGCAGC CGCCTTCACA 
GACCATGTGG AGTGTCTGCA GCTTCTGCTC AGCCATAATG CTCACGTGAA CTCTGTGGAC 
TCCTCTGGGA AAACACCTCT CATGATGGCT GCAGAAAACG GACAGACAAA CACAGTTGAG 
ATGCTGGTTA GCAGTGCTAG TGCAGATCTG ACTTTACAAG ATAACAGTAA AAACACCGCC 
CTCCATTTGG CTTGCAGTAA GGGTCATGAA ACTAGTGCCT TGTTAATACT GGAAAAGATA 
ACAGATAGAA ACCTCATCAA TGCAACCAAT GCAGCTTTGC AAACACCTCT GCATGTTGCT 
GCCCGAAACG GGCTGACAAT GGTGGTTCAG GAACTCCTGG GGAAAGGGGC AAGCGTGCTT 
GCGGTCGATG AGAACGGCTA TACCCCAGCT TTGGCCTGTG CTCCCAACAA GGACGTTGCA 
GACTGCCTCG CACTCATTCT GGCCACCATG ATGCCTATCT CGTCAAGTAG CCCTTTATCA 
TCCCTGACGT TCAATGCCAT TAACCGTTAC ACCAACACCT CGAAAACTGT CAGCTTCGAA 
GCCTTGCCCA TCATGAGAAA TGAACCCAGC TCCTACTGTA GCTTCAACAA CATCGGCGGG 
GAGCAGGAGT ACCTATACAC TGATGTGGAC GAGCTCAATG ACTCCGATTC AGAGACCTAC 
TGA