DBR1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	DBR1
Entrez Gene ID	109559954
Full Name	debranching RNA lariats 1
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019962003.1	XP_019817562.1	lariat debranching enzyme

Pathways from BioSystems
Gene Ontology

The following DBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo26717	XM_019962003.1 Latest version!	Bos indicus debranching RNA lariats 1 (DBR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBR1 ( XM_019962003.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019817562.1 Bos indicus debranching RNA lariats 1 (DBR1), mRNA.

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CloneID	OBo26717
Clone ID Related Accession (Same CDS sequence)	XM_019962003.1
Accession Version	XM_019962003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	lariat debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032650.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGTGG CTGTGGCCGG CTGTTGCCAC GGCGAGCTGG ACAAGATCTA TGAGACGCTG 
GCGCTGGCGG AGCGGCGCGG CCCGGGGCGG ATAGACCTTC TGCTCTGCTG CGGCGACTTC 
CAGGCAGTGC GCAACGAGGC CGACCTGCGT TGCATGGCCG TGCCGCCCAA GTACCGCCAC 
ATGCAAACCT TCTACAGGTA TTACTCTGGG GAGAAGAAGG CCCCAGTTCT CACGATTTTC 
ATTGGGGGAA ACCATGAAGC CTCAAATCAT TTGCAAGAGT TACCCTACGG TGGCTGGGTA 
GCACCAAACA TTTATTATTT AGGTTTGGCA GGAGTAGTAA AATACCGAGG AGTAAGAATT 
GGTGGAATCT CTGGTATCTT TAAATCTCAT GACTATCGAA AAGGTCATTT TGAGTGCCCC 
CCTTATAATG CAGCTACAGT AAGGAGTATA TATCATGTGA GAAATATTGA AGTCTACAAA 
TTAAAACAGC TGAAGCAGCC TATGGACATA TTCTTATCTC ATGATTGGCC AAGAAGTATC 
TATCATTATG GAAACAAGAA GCAACTTCTT AAGACTAAAT CTTTTTTCCG ACAAGAAGTG 
GAAAATAACA CACTAGGAAG TCCCGCTGCC TCCGAGCTTT TAGAGCACTT AAAACCTACT 
TACTGGTTTT CCGCCCACCT ACATGTAAAG TTTGCAGCCT TGATGCAGCA CCAGACCGTG 
GATAAAGGAC AGTCAACCAA AGCAACCAAA TTTCTAGCCT TGGACAAGTG CTTACCACAC 
AGAGACTTTC TTCAGGTAAT AGAAGTAGAA CATGACCCCA GTGCTCCTGA TTACTTGGAA 
TATGATGCTG AATGGCTCAC TGTTCTCAGG GCTACTGATG ATCTTATTAA TGTGACTGAG 
CGCCTGTGGA ATATGCCTGA GAATAATGGC CTACATACAA GATGGGATTA CAGTGCAACA 
AAAGAAGCTA TTAACGAAGT ACTGGAGAAA TTGAATCATG ACCTCAAAGT TCCAAATAAC 
TTTAGTATAA CAGCTGCTTG TTATGACCCT AGCAAGCCAC AGACACAGAT GCAGTTGGTT 
CATAGGATCA GTCCTCAGAC GACTGAATTT TGTGCCCAAC TTGGCATCAC AGACATTAAC 
GTCAGGCTTC AGAAGGCCAA GGAAGACCAG CACTTGTGTG GTGACTATGA AGGGCAGGAT 
GACACGGAGG GTAATGACTC TGGAGAAGAC CCTAGTGAAT ATAACACAGA CACATCTGCC 
CTGTCCTCTA TTAATCCAGA TGAGATCATG TTAGATGAAG AAGAGGAGTA TGATGATAGT 
ATGGTAAGTG CACACAGTGA CATGAATACA CCGTCTGTAG AACCGTCTTG TGATCAAGCT 
TCTGACTTTT CTGCAAGCTT CTCTGACATC AGGATCTTGC CAAGTTCCAT GACTGTATCT 
TCTGACGATA CATCGGGTTC CCCAGTGTGC AAGGAGGGGA ATTCTGGTGA CCCTGTGGAC 
TTGGGGGATG GAAAGGACTT AACCACGGTG CCACTGAAGA GGCTGAGTGA TGAACATGAA 
CCTGAACAAA GAAAGAAAAT CAAGAGAAGG AATCAAGCCA TCTATGCCGC AGTGGATGAT 
AATGATACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019817562.1
CDS	262..1896
Translation	MRVAVAGCCHGELDKIYETLALAERRGPGRIDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSGEKKAPVLTIFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECPPYNAATVRSIYHVRNIEVYKLKQLKQPMDIFLSHDWPRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAASELLEHLKPTYWFSAHLHVKFAALMQHQTVDKGQSTKATKFLALDKCLPHRDFLQVIEVEHDPSAPDYLEYDAEWLTVLRATDDLINVTERLWNMPENNGLHTRWDYSATKEAINEVLEKLNHDLKVPNNFSITAACYDPSKPQTQMQLVHRISPQTTEFCAQLGITDINVRLQKAKEDQHLCGDYEGQDDTEGNDSGEDPSEYNTDTSALSSINPDEIMLDEEEEYDDSMVSAHSDMNTPSVEPSCDQASDFSASFSDIRILPSSMTVSSDDTSGSPVCKEGNSGDPVDLGDGKDLTTVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDNDTA

Target ORF information:

RefSeq Version	XM_019962003.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus debranching RNA lariats 1 (DBR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019962003.1

ORF Insert Sequence:

ATGCGCGTGG CTGTGGCCGG CTGTTGCCAC GGCGAGCTGG ACAAGATCTA TGAGACGCTG 
GCGCTGGCGG AGCGGCGCGG CCCGGGGCGG ATAGACCTTC TGCTCTGCTG CGGCGACTTC 
CAGGCAGTGC GCAACGAGGC CGACCTGCGT TGCATGGCCG TGCCGCCCAA GTACCGCCAC 
ATGCAAACCT TCTACAGGTA TTACTCTGGG GAGAAGAAGG CCCCAGTTCT CACGATTTTC 
ATTGGGGGAA ACCATGAAGC CTCAAATCAT TTGCAAGAGT TACCCTACGG TGGCTGGGTA 
GCACCAAACA TTTATTATTT AGGTTTGGCA GGAGTAGTAA AATACCGAGG AGTAAGAATT 
GGTGGAATCT CTGGTATCTT TAAATCTCAT GACTATCGAA AAGGTCATTT TGAGTGCCCC 
CCTTATAATG CAGCTACAGT AAGGAGTATA TATCATGTGA GAAATATTGA AGTCTACAAA 
TTAAAACAGC TGAAGCAGCC TATGGACATA TTCTTATCTC ATGATTGGCC AAGAAGTATC 
TATCATTATG GAAACAAGAA GCAACTTCTT AAGACTAAAT CTTTTTTCCG ACAAGAAGTG 
GAAAATAACA CACTAGGAAG TCCCGCTGCC TCCGAGCTTT TAGAGCACTT AAAACCTACT 
TACTGGTTTT CCGCCCACCT ACATGTAAAG TTTGCAGCCT TGATGCAGCA CCAGACCGTG 
GATAAAGGAC AGTCAACCAA AGCAACCAAA TTTCTAGCCT TGGACAAGTG CTTACCACAC 
AGAGACTTTC TTCAGGTAAT AGAAGTAGAA CATGACCCCA GTGCTCCTGA TTACTTGGAA 
TATGATGCTG AATGGCTCAC TGTTCTCAGG GCTACTGATG ATCTTATTAA TGTGACTGAG 
CGCCTGTGGA ATATGCCTGA GAATAATGGC CTACATACAA GATGGGATTA CAGTGCAACA 
AAAGAAGCTA TTAACGAAGT ACTGGAGAAA TTGAATCATG ACCTCAAAGT TCCAAATAAC 
TTTAGTATAA CAGCTGCTTG TTATGACCCT AGCAAGCCAC AGACACAGAT GCAGTTGGTT 
CATAGGATCA GTCCTCAGAC GACTGAATTT TGTGCCCAAC TTGGCATCAC AGACATTAAC 
GTCAGGCTTC AGAAGGCCAA GGAAGACCAG CACTTGTGTG GTGACTATGA AGGGCAGGAT 
GACACGGAGG GTAATGACTC TGGAGAAGAC CCTAGTGAAT ATAACACAGA CACATCTGCC 
CTGTCCTCTA TTAATCCAGA TGAGATCATG TTAGATGAAG AAGAGGAGTA TGATGATAGT 
ATGGTAAGTG CACACAGTGA CATGAATACA CCGTCTGTAG AACCGTCTTG TGATCAAGCT 
TCTGACTTTT CTGCAAGCTT CTCTGACATC AGGATCTTGC CAAGTTCCAT GACTGTATCT 
TCTGACGATA CATCGGGTTC CCCAGTGTGC AAGGAGGGGA ATTCTGGTGA CCCTGTGGAC 
TTGGGGGATG GAAAGGACTT AACCACGGTG CCACTGAAGA GGCTGAGTGA TGAACATGAA 
CCTGAACAAA GAAAGAAAAT CAAGAGAAGG AATCAAGCCA TCTATGCCGC AGTGGATGAT 
AATGATACAG CCTGA