PIFO cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIFO
Entrez Gene ID	103116985
Full Name	primary cilia formation
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007526954.1	XP_007527016.1	protein pitchfork

Pathways from BioSystems
Gene Ontology

The following PIFO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIFO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr14983	XM_007526954.1 Latest version!	Erinaceus europaeus primary cilia formation (PIFO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIFO ( XM_007526954.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007527016.1 Erinaceus europaeus primary cilia formation (PIFO), mRNA.

Price & Availability↑

CloneID	OEr14983
Clone ID Related Accession (Same CDS sequence)	XM_007526954.1
Accession Version	XM_007526954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	protein pitchfork
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804245.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 26% of CDS bases ##RefSeq-Attributes-END##

ATGGAGAAAA GAATTGAGAA ACACAAGCTT TCTGAACTTC TCTTTGATAT AGCAGTCTGG 
CCTAAAACTT CTACTACAGC TTCATCAGAT TCCCAGAATC CTTATGATCT ACTTGGGAAC 
ATAAGGGTCA GCAAAACCAT ATCATATATT ACCCAGCCAT TCTTGTGGAA ATTTTCGGAT 
GGTGCACTAT GCAATTATCC GTTTGGGACA TGTCAGCAGA GGAAGCTCTT TCCTCACTAT 
TATCCCCTAA CCTTCTTTGG GAACAAGTTT GTTCCTCTTA GAGGATCACC CCACAGAGGG 
CCTGGATGCT ACACAAAAGA TATTTATGGT TTGGCATACA GCCTCGCTAA GGTTCCGACC 
AGTAAAAAAG GATATACACT TGGAGCCAGG ACAGCCCCAA GATTTCAATC AATCAACAAG 
AATGTGACTC CTTACCCAGG CATGTACCAG GCAGAATACT CTGAGGAAAA AAAGAAAAAA 
CAGAGTTTTG CTCCATTTAA TGTCTTTGCA CCTCGGTTTA GGGAAGACTC AAAAAACATT 
CCTGGTCCTG GTACATACAA CCCAATTATA AAGCCACCCG CCAAAATCAC TTGGCCAATG 
AAATTTGGAT CTCCAGATTG GGCTCAGGTT CCATGTCTAC AGAAAAGAAC CCTAAAAACT 
GAGCTGTCCA CAGACAAAGA CTTCAGAAAG CACCGGAACC GTGTGGCTTA CCTAAGCCTG 
TATTACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007527016.1
CDS	1..732
Translation	MEKRIEKHKLSELLFDIAVWPKTSTTASSDSQNPYDLLGNIRVSKTISYITQPFLWKFSDGALCNYPFGTCQQRKLFPHYYPLTFFGNKFVPLRGSPHRGPGCYTKDIYGLAYSLAKVPTSKKGYTLGARTAPRFQSINKNVTPYPGMYQAEYSEEKKKKQSFAPFNVFAPRFREDSKNIPGPGTYNPIIKPPAKITWPMKFGSPDWAQVPCLQKRTLKTELSTDKDFRKHRNRVAYLSLYYN

Target ORF information:

RefSeq Version	XM_007526954.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus primary cilia formation (PIFO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007526954.1

ORF Insert Sequence:

ATGGAGAAAA GAATTGAGAA ACACAAGCTT TCTGAACTTC TCTTTGATAT AGCAGTCTGG 
CCTAAAACTT CTACTACAGC TTCATCAGAT TCCCAGAATC CTTATGATCT ACTTGGGAAC 
ATAAGGGTCA GCAAAACCAT ATCATATATT ACCCAGCCAT TCTTGTGGAA ATTTTCGGAT 
GGTGCACTAT GCAATTATCC GTTTGGGACA TGTCAGCAGA GGAAGCTCTT TCCTCACTAT 
TATCCCCTAA CCTTCTTTGG GAACAAGTTT GTTCCTCTTA GAGGATCACC CCACAGAGGG 
CCTGGATGCT ACACAAAAGA TATTTATGGT TTGGCATACA GCCTCGCTAA GGTTCCGACC 
AGTAAAAAAG GATATACACT TGGAGCCAGG ACAGCCCCAA GATTTCAATC AATCAACAAG 
AATGTGACTC CTTACCCAGG CATGTACCAG GCAGAATACT CTGAGGAAAA AAAGAAAAAA 
CAGAGTTTTG CTCCATTTAA TGTCTTTGCA CCTCGGTTTA GGGAAGACTC AAAAAACATT 
CCTGGTCCTG GTACATACAA CCCAATTATA AAGCCACCCG CCAAAATCAC TTGGCCAATG 
AAATTTGGAT CTCCAGATTG GGCTCAGGTT CCATGTCTAC AGAAAAGAAC CCTAAAAACT 
GAGCTGTCCA CAGACAAAGA CTTCAGAAAG CACCGGAACC GTGTGGCTTA CCTAAGCCTG 
TATTACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.