LOC103028904 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103028904
Entrez Gene ID	103028904
Full Name	dentin sialophosphoprotein-like
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007254617.2	XP_007254679.1	dentin sialophosphoprotein-like
XM_022678123.1	XP_022533844.1	dentin sialophosphoprotein-like

Pathways from BioSystems
Gene Ontology

The following LOC103028904 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103028904 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs110992	XM_022678123.1 Latest version!	Astyanax mexicanus dentin sialophosphoprotein-like (LOC103028904), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAs36678	XM_007254617.2 Latest version!	Astyanax mexicanus dentin sialophosphoprotein-like (LOC103028904), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103028904 ( XM_022678123.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022533844.1 Astyanax mexicanus dentin sialophosphoprotein-like (LOC103028904), mRNA.

Price & Availability↑

CloneID	OAs110992
Clone ID Related Accession (Same CDS sequence)	XM_022678123.1
Accession Version	XM_022678123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Astyanax mexicanus(Mexican tetra)
Product	dentin sialophosphoprotein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCT TTATCCTGGA CTGCCTGCGC GTGGGACGGC GACGCGCGGC CTGCGAAAGA 
GAAAACTCAG TGGAACTGCA AACGAAGGAC AAAGATGCTG CCAACGATAA TGAAATCAAG 
GTACTGGAGG ATGTGGTGGC TATGGAAGCA GGAACACTGC AGAAGGAGAG GGTGCTGTCC 
CAGGATGCCC AGCAGGCCCT GGAGAGACTT ACGAAACGGA GAACCCAGCT GAAGGAGGAA 
ATAGAGGGCA TTCAGGAGCA GCGCAAACAG GAGGGCCAGC TGCTTCTGTC ACTGCAGGAG 
GAGCAGGAGG AGCTGGAGCA GATGGTGCTG GAGTACGAGG TGGAGCTGGA GAGAGCTAAC 
GATGAGCTAC AGCACCTCCA TGAGGAGGTC AGGAAGACCA GAGCAAGGGT GGAAGATGCA 
CATGGTCGAA TGGGTCCACT CAAACACTCC ATCGGTGAAA CATACACAGA AATCACTGAG 
ACTAAAGAGA GATTAGGTGA ACTGATTGCT GAGATGATTG CCATTGAAGC TTGTGTTCCT 
GAGATTACCT GCCATCAGAT GGCTGAGCAC TTGAGCATAG ACCTTGATAA CAGTTCAAGA 
GAAGAAGAAG ATGATGATGA TGAAGAGTGC TATGGGCATG CACATGAGGA TGAAAACACA 
ATCAAGAAAA TCACTGTGGA GAAATATCAT TCTGAGCAGG AACAAAACAT AGAGGGATCT 
GAAACACTGC AGCGTTCTGC AGAAAACTTG AAGAGCAGTG GTTCTTCTTC GTTTACTGAG 
ATTACATTAA CTGAAGTTAA AGAAGAGGAC GTGTCCCCCA GATCTAACAC ACCACAGCAC 
ATGGACTCTC CTGATTTGGA AGAAGATTTT GAGATAGTCC AGTCTGTCGA CACAAAGCCA 
ATTAACCAGA CCTCAAGCAG GGAGTTTGAC TTCTTCCACC CAGACCCTTT TGTTGATCAT 
GATGTGTTTG GAGATGACAG ATTCCCAAAA GTAGACATGA CAGAGTTCCT GTCTGGTGAT 
CCATTCAAAG GCACAGATCC TTTTGCTTCC GATATCCTTT TCAACGATGT AGCAGAAGCC 
CAGTTCTCTC AGGATTACCC TCACAATGAA GAAAGCAACA CCAATAAAGA TGAACATGCT 
GATGCAGCTA ATGCTGTTAT ACATAGTTCT GCCAATGCTG ACTGTCAGTC CATTGACTCT 
GGAATGTTTG AACAAAATAT TCCTGAGCCC AGTGACTCTG AAAGGTACAC TCCGTACAGC 
GACGTCTGTC CAGATGTTGG ATCCAACAAC ACAGTAAGCC AAGAGCTTGA CAGCATGGAA 
CTAAATAACA CCTCAGGAAC CGAATTCGAT CTTCCTGCAA GTTCCCAGCA TGACGATGAA 
GGGCGATTTG ACCCGATCCA TTGTGAACTT CACGACAGCC ATGAATCTGA TTCCAGTGAA 
TCTTCCACAG TTGATAGTTC CTTTGATGGA GATTTTGGTA CATTCACTGG ACTGGAGCAA 
ACAGAACGTG AGCCAGAACC GGATGTGGGA CCCAGTTATG TTAGAAGTAA TCGAGCTTTG 
TATGGTCTTC AGCGTATTGA CACAAGTGCA TACACTAGTT TTCCTCTTAG TCCTGATGAC 
TTTGCTCCAG AACAAGATGT TCCTGTCGCC AGTGATAAAG ACAAACCTGT TGAAGGTGCT 
GATCAATTTT CCTTCCATTC TGATCACGAT GATGCAGTCA GTGAAGTGAA GCCTGAGGTT 
GAAGAAGCTC TTTCTCCTGA GGGAAATCCT GCTAGCCCAA GTCCTCTTGA CCAAGAGTTT 
GACAAGCCCT GTGATGACTA CATTCAGGAA ATTCCTGCAG TCCATAGTCC AGAGCCTTCT 
GAAGTAGATG TTTATGACCA CAAATTTGGA GATATATATT TCTTTACAGT GCGGTTAGAC 
CCTGGTAGTC CAGATGCTAG CCCTGGTTCT GCCGAAGCCA ATGAGCAAAA GAAATCTGAA 
TGTGACCAAA CAAGTCCTGA ATCTAATGAA GCGGACGAAT TTGACTCTGC TGCTCCAGAA 
GAAGAGGATA CTAATCACTA TAATCCTGAA GCATATTTGG AGGAAAGTTT TGACAAAGAA 
GATTCTTCGA TCAGAACAGA AGAAACCCTG AGTCCTGAAC CAAATGATCC AGAACAGTCT 
GAACCTGAGG TAAGCCCCAC AGGATACAGC CCAAATTTTG AGCAGGAAGA TGAGGATAAT 
GGTCTTGATG AGTTTGGACA TAGTATATTT AATGTAGAGA GCAGCAGTGT ACTGAAAAAC 
ACTGAAAATG AAATACCTGA ATCTTTTCAC CCCGAAAATG TTGACTCTGA AAATGAAATA 
CCTGAAAAAG TTGACCCTGC CTATACTGAA GACTATTCAG TCCACTTTAC CACAGAACCT 
GTTGATCAGT TTCTCATCAG CCCTGTCTCT GGAGACACAG AAAAGGCAGC TTCTAACAGT 
TCCAAGCTTT GTTCTCATAA CCCATTCAGC CTTGACTCTG AAAGCATAGA TCACTGTGAT 
GACGCTGAAA GCAATCTTCA TCCTTTTAAC ACCAAAAACT GCACTGGAAA AGAGTCTTAT 
GGCTGTGTCA ATGAGCTTAA ATCTTTTGAC CCATTCGATC ATGTACTTAA CGAAAACACA 
ATATCAGTCA AATCGGCCAA TTCTACATGT ACTGACAGTG GAAGACTAGA TTCCGGCTAT 
CATGAAAATA ATGGGCTGGA TCCTTTCAGT CCTGCACCTT CACATCCTTC TACAGACATG 
GGTCCATGCT TTGTGAGTTC CAACACAGAG ATGCTCAGTC CTGGTCTTTT GGACCTGGAG 
GCTCCTGATC CCTTCAGCCC TGAGTCCATT GGGTCCAGTA GTACAATGAG ATTTCACTCT 
GAACTCTTCA ACCCTTCCAC CGACGATGGA ATGATTGGAG ATCCATGTCA GGTATATACA 
GCCTCGTCCG ACATCCTTGG GGATTCCTTT AGTGGGTCAG AACTTGACAG TTTAGACCCT 
TTCAGTCCGG TGCCACACTT GACAGGGGGA AGTTACAGTC ATTGCAATGT AGACATTCTT 
GTCCCTGGCT TGATTCAGAA CAATTCAAAA TTTGATGGAG AAACTAGATC CAGCAGCTGT 
GATTCAGGGG TTGGTTCTGC TCAAAATAGA GAAGGTTCCC AGCCACCAAT CCACTCTGAG 
GTCAATAACT TTGTTTATTT GCCCAATTGC CCAACAAAGG ACTCTTTTTT TGAGTCGGAT 
AACAGTAACA TGGTTACAGA TGATCATGCT TTCCAATCAA AAGTGGAGAA CGTATCAGAA 
CAGGAACAGA ACACAAATTC TTCAGCAAAT GACACATCGA TGGCAGAACT CACCGAAATA 
GATTTTTTCT GCAATGAGCT CAGCAAAATG GTGGCCTCAC GTTCCTCTGA TACTGTTCAG 
CAAAGTGTTG CTGAAATGCT GTTCGGCTCT AACCCTGACA CCGCAAGCTT CTACCCATGG 
GATTTCGAGA ACAGCGACAC AGTTGCCAAC AATTACTCAA GTCCAGAAGC CGATTTAGTG 
GACAACAGCT ACAACTACAG CAAGGATCTG CCGAAGTTGG AGTCAGGAAC CAGATCACGT 
AGGTCCAGTG ACCTTCTAGG TGAACTTCAG AGCGCTGCTG GACTTGCACA TGATGAAACT 
GAAAAGCCCT GCTTGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022533844.1
CDS	197..3877
Translation	MMRFILDCLRVGRRRAACERENSVELQTKDKDAANDNEIKVLEDVVAMEAGTLQKERVLSQDAQQALERLTKRRTQLKEEIEGIQEQRKQEGQLLLSLQEEQEELEQMVLEYEVELERANDELQHLHEEVRKTRARVEDAHGRMGPLKHSIGETYTEITETKERLGELIAEMIAIEACVPEITCHQMAEHLSIDLDNSSREEEDDDDEECYGHAHEDENTIKKITVEKYHSEQEQNIEGSETLQRSAENLKSSGSSSFTEITLTEVKEEDVSPRSNTPQHMDSPDLEEDFEIVQSVDTKPINQTSSREFDFFHPDPFVDHDVFGDDRFPKVDMTEFLSGDPFKGTDPFASDILFNDVAEAQFSQDYPHNEESNTNKDEHADAANAVIHSSANADCQSIDSGMFEQNIPEPSDSERYTPYSDVCPDVGSNNTVSQELDSMELNNTSGTEFDLPASSQHDDEGRFDPIHCELHDSHESDSSESSTVDSSFDGDFGTFTGLEQTEREPEPDVGPSYVRSNRALYGLQRIDTSAYTSFPLSPDDFAPEQDVPVASDKDKPVEGADQFSFHSDHDDAVSEVKPEVEEALSPEGNPASPSPLDQEFDKPCDDYIQEIPAVHSPEPSEVDVYDHKFGDIYFFTVRLDPGSPDASPGSAEANEQKKSECDQTSPESNEADEFDSAAPEEEDTNHYNPEAYLEESFDKEDSSIRTEETLSPEPNDPEQSEPEVSPTGYSPNFEQEDEDNGLDEFGHSIFNVESSSVLKNTENEIPESFHPENVDSENEIPEKVDPAYTEDYSVHFTTEPVDQFLISPVSGDTEKAASNSSKLCSHNPFSLDSESIDHCDDAESNLHPFNTKNCTGKESYGCVNELKSFDPFDHVLNENTISVKSANSTCTDSGRLDSGYHENNGLDPFSPAPSHPSTDMGPCFVSSNTEMLSPGLLDLEAPDPFSPESIGSSSTMRFHSELFNPSTDDGMIGDPCQVYTASSDILGDSFSGSELDSLDPFSPVPHLTGGSYSHCNVDILVPGLIQNNSKFDGETRSSSCDSGVGSAQNREGSQPPIHSEVNNFVYLPNCPTKDSFFESDNSNMVTDDHAFQSKVENVSEQEQNTNSSANDTSMAELTEIDFFCNELSKMVASRSSDTVQQSVAEMLFGSNPDTASFYPWDFENSDTVANNYSSPEADLVDNSYNYSKDLPKLESGTRSRRSSDLLGELQSAAGLAHDETEKPCLL

Target ORF information:

RefSeq Version	XM_022678123.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus dentin sialophosphoprotein-like (LOC103028904), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022678123.1

ORF Insert Sequence:

ATGATGCGCT TTATCCTGGA CTGCCTGCGC GTGGGACGGC GACGCGCGGC CTGCGAAAGA 
GAAAACTCAG TGGAACTGCA AACGAAGGAC AAAGATGCTG CCAACGATAA TGAAATCAAG 
GTACTGGAGG ATGTGGTGGC TATGGAAGCA GGAACACTGC AGAAGGAGAG GGTGCTGTCC 
CAGGATGCCC AGCAGGCCCT GGAGAGACTT ACGAAACGGA GAACCCAGCT GAAGGAGGAA 
ATAGAGGGCA TTCAGGAGCA GCGCAAACAG GAGGGCCAGC TGCTTCTGTC ACTGCAGGAG 
GAGCAGGAGG AGCTGGAGCA GATGGTGCTG GAGTACGAGG TGGAGCTGGA GAGAGCTAAC 
GATGAGCTAC AGCACCTCCA TGAGGAGGTC AGGAAGACCA GAGCAAGGGT GGAAGATGCA 
CATGGTCGAA TGGGTCCACT CAAACACTCC ATCGGTGAAA CATACACAGA AATCACTGAG 
ACTAAAGAGA GATTAGGTGA ACTGATTGCT GAGATGATTG CCATTGAAGC TTGTGTTCCT 
GAGATTACCT GCCATCAGAT GGCTGAGCAC TTGAGCATAG ACCTTGATAA CAGTTCAAGA 
GAAGAAGAAG ATGATGATGA TGAAGAGTGC TATGGGCATG CACATGAGGA TGAAAACACA 
ATCAAGAAAA TCACTGTGGA GAAATATCAT TCTGAGCAGG AACAAAACAT AGAGGGATCT 
GAAACACTGC AGCGTTCTGC AGAAAACTTG AAGAGCAGTG GTTCTTCTTC GTTTACTGAG 
ATTACATTAA CTGAAGTTAA AGAAGAGGAC GTGTCCCCCA GATCTAACAC ACCACAGCAC 
ATGGACTCTC CTGATTTGGA AGAAGATTTT GAGATAGTCC AGTCTGTCGA CACAAAGCCA 
ATTAACCAGA CCTCAAGCAG GGAGTTTGAC TTCTTCCACC CAGACCCTTT TGTTGATCAT 
GATGTGTTTG GAGATGACAG ATTCCCAAAA GTAGACATGA CAGAGTTCCT GTCTGGTGAT 
CCATTCAAAG GCACAGATCC TTTTGCTTCC GATATCCTTT TCAACGATGT AGCAGAAGCC 
CAGTTCTCTC AGGATTACCC TCACAATGAA GAAAGCAACA CCAATAAAGA TGAACATGCT 
GATGCAGCTA ATGCTGTTAT ACATAGTTCT GCCAATGCTG ACTGTCAGTC CATTGACTCT 
GGAATGTTTG AACAAAATAT TCCTGAGCCC AGTGACTCTG AAAGGTACAC TCCGTACAGC 
GACGTCTGTC CAGATGTTGG ATCCAACAAC ACAGTAAGCC AAGAGCTTGA CAGCATGGAA 
CTAAATAACA CCTCAGGAAC CGAATTCGAT CTTCCTGCAA GTTCCCAGCA TGACGATGAA 
GGGCGATTTG ACCCGATCCA TTGTGAACTT CACGACAGCC ATGAATCTGA TTCCAGTGAA 
TCTTCCACAG TTGATAGTTC CTTTGATGGA GATTTTGGTA CATTCACTGG ACTGGAGCAA 
ACAGAACGTG AGCCAGAACC GGATGTGGGA CCCAGTTATG TTAGAAGTAA TCGAGCTTTG 
TATGGTCTTC AGCGTATTGA CACAAGTGCA TACACTAGTT TTCCTCTTAG TCCTGATGAC 
TTTGCTCCAG AACAAGATGT TCCTGTCGCC AGTGATAAAG ACAAACCTGT TGAAGGTGCT 
GATCAATTTT CCTTCCATTC TGATCACGAT GATGCAGTCA GTGAAGTGAA GCCTGAGGTT 
GAAGAAGCTC TTTCTCCTGA GGGAAATCCT GCTAGCCCAA GTCCTCTTGA CCAAGAGTTT 
GACAAGCCCT GTGATGACTA CATTCAGGAA ATTCCTGCAG TCCATAGTCC AGAGCCTTCT 
GAAGTAGATG TTTATGACCA CAAATTTGGA GATATATATT TCTTTACAGT GCGGTTAGAC 
CCTGGTAGTC CAGATGCTAG CCCTGGTTCT GCCGAAGCCA ATGAGCAAAA GAAATCTGAA 
TGTGACCAAA CAAGTCCTGA ATCTAATGAA GCGGACGAAT TTGACTCTGC TGCTCCAGAA 
GAAGAGGATA CTAATCACTA TAATCCTGAA GCATATTTGG AGGAAAGTTT TGACAAAGAA 
GATTCTTCGA TCAGAACAGA AGAAACCCTG AGTCCTGAAC CAAATGATCC AGAACAGTCT 
GAACCTGAGG TAAGCCCCAC AGGATACAGC CCAAATTTTG AGCAGGAAGA TGAGGATAAT 
GGTCTTGATG AGTTTGGACA TAGTATATTT AATGTAGAGA GCAGCAGTGT ACTGAAAAAC 
ACTGAAAATG AAATACCTGA ATCTTTTCAC CCCGAAAATG TTGACTCTGA AAATGAAATA 
CCTGAAAAAG TTGACCCTGC CTATACTGAA GACTATTCAG TCCACTTTAC CACAGAACCT 
GTTGATCAGT TTCTCATCAG CCCTGTCTCT GGAGACACAG AAAAGGCAGC TTCTAACAGT 
TCCAAGCTTT GTTCTCATAA CCCATTCAGC CTTGACTCTG AAAGCATAGA TCACTGTGAT 
GACGCTGAAA GCAATCTTCA TCCTTTTAAC ACCAAAAACT GCACTGGAAA AGAGTCTTAT 
GGCTGTGTCA ATGAGCTTAA ATCTTTTGAC CCATTCGATC ATGTACTTAA CGAAAACACA 
ATATCAGTCA AATCGGCCAA TTCTACATGT ACTGACAGTG GAAGACTAGA TTCCGGCTAT 
CATGAAAATA ATGGGCTGGA TCCTTTCAGT CCTGCACCTT CACATCCTTC TACAGACATG 
GGTCCATGCT TTGTGAGTTC CAACACAGAG ATGCTCAGTC CTGGTCTTTT GGACCTGGAG 
GCTCCTGATC CCTTCAGCCC TGAGTCCATT GGGTCCAGTA GTACAATGAG ATTTCACTCT 
GAACTCTTCA ACCCTTCCAC CGACGATGGA ATGATTGGAG ATCCATGTCA GGTATATACA 
GCCTCGTCCG ACATCCTTGG GGATTCCTTT AGTGGGTCAG AACTTGACAG TTTAGACCCT 
TTCAGTCCGG TGCCACACTT GACAGGGGGA AGTTACAGTC ATTGCAATGT AGACATTCTT 
GTCCCTGGCT TGATTCAGAA CAATTCAAAA TTTGATGGAG AAACTAGATC CAGCAGCTGT 
GATTCAGGGG TTGGTTCTGC TCAAAATAGA GAAGGTTCCC AGCCACCAAT CCACTCTGAG 
GTCAATAACT TTGTTTATTT GCCCAATTGC CCAACAAAGG ACTCTTTTTT TGAGTCGGAT 
AACAGTAACA TGGTTACAGA TGATCATGCT TTCCAATCAA AAGTGGAGAA CGTATCAGAA 
CAGGAACAGA ACACAAATTC TTCAGCAAAT GACACATCGA TGGCAGAACT CACCGAAATA 
GATTTTTTCT GCAATGAGCT CAGCAAAATG GTGGCCTCAC GTTCCTCTGA TACTGTTCAG 
CAAAGTGTTG CTGAAATGCT GTTCGGCTCT AACCCTGACA CCGCAAGCTT CTACCCATGG 
GATTTCGAGA ACAGCGACAC AGTTGCCAAC AATTACTCAA GTCCAGAAGC CGATTTAGTG 
GACAACAGCT ACAACTACAG CAAGGATCTG CCGAAGTTGG AGTCAGGAAC CAGATCACGT 
AGGTCCAGTG ACCTTCTAGG TGAACTTCAG AGCGCTGCTG GACTTGCACA TGATGAAACT 
GAAAAGCCCT GCTTGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103028904 ( XM_007254617.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007254679.1 Astyanax mexicanus dentin sialophosphoprotein-like (LOC103028904), mRNA.

Price & Availability↑

CloneID	OAs36678
Clone ID Related Accession (Same CDS sequence)	XM_007254617.2
Accession Version	XM_007254617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-10
Organism	Astyanax mexicanus(Mexican tetra)
Product	dentin sialophosphoprotein-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749377.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007254617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAGAGAAA ACTCAGTGGA ACTGCAAACG AAGGACAAAG ATGCTGCCAA CGATAATGAA 
ATCAAGGTAC TGGAGGATGT GGTGGCTATG GAAGCAGGAA CACTGCAGAA GGAGAGGGTG 
CTGTCCCAGG ATGCCCAGCA GGCCCTGGAG AGACTTACGA AAAGGAGAAC CCAGCTGAAG 
GAGGAAATAG AGGGCATTCA GGAGCAGCGC AAACAGGAGG GCCAGCTGCT TCTGTCACTG 
CAGGAGGAGC AGGAGGAGCT GGAGCAGATG GTGCAGGAGT ACGAGGTGGA GCTGGAGAGA 
GCTAACGATG AGCTACAGCA CCTCCATGAG GAGGTCAGGA AGACCAGAGC AAGGGTGGAA 
GATGCACATG GTCGAATGGG TCCACTCAAA CACTCCATCG GGGAAACATA CACAGAAATC 
ACTGAGACTA AAGAGAGATT AGGTGAACTG ATTGCTGAGA TGATTGCCAT TGAAGCTTGT 
GTTCCTGAGA TTACCTGCCA TCAGATGGCT GAGCACTTGA GCATAGACCT TGATAACAGT 
TCAAGAGAAG AAGAAGATGA TGATGATGAA GAGTGCTATG GGCATGCACA TGAGGATGAA 
AACACAATCA AGAAAATCAC TGTGGAGAAA TATCATTCTG AGCAGGAACA AAACATAGAG 
GGATCTGAAA CACTGCAGCG TTCTGCAGAA AACTTGAAGA GCAGTGGTTC TTCTTCGTTT 
ACTGAGATTA CATTAACTGA AGTTAAAGAA GAGGACGTGT CCCCCAGATC TAACACACCA 
CAGCACATGG ACTCTCCTGA TTTGGAAGAA GATTTTGAGA TAGTCCAGTC TGTCGACACA 
AAGCCAATTA ACCAGACCTC AAGCAGGGAG TTTGACTTCT TCCACCCAGA CCCTTTTGTT 
GATCATGATG TGTTTGGAGA TGACAGATTC CCAAAAGTAG ACATGACAGA GTTCCTGTCT 
GGTGATCCAT TCAAAGGCAC AGATCCTTTT GCTTCCGATA TCCTTTTCAA CGATGTAGCA 
GATGCCCAGT TCTCTCAGGA TTACCCTCAC AATGAAGAAA GCAACACCAA TAAAGATGAA 
CATGCTGATG CAGCTAATGC TGTTATACAT AGTTCTGCCA ATGCTGACTG TCAGTCCATT 
GACTCTGGAA TGTTTGAACA AAATATTCCT GAGCCCAGTG ACTCTGAAAG GTACACTCCG 
TACAGCGACG TCTGTCCAGA TGTTGGATCC AACAACACAG TAAGCCAAGA GCTTGACAGC 
ATGGAACTAA ATAACACCTC AGGAACCGAA TTCGATCTTC CTGCAAGTTC CCAGCTTGAC 
GATGAAGGGC GATTTGACCC GATCCATTGT GAACTTCACG ACAGCCATGA ATCTGATTCC 
AGTGAATCTT CCACAGTTGA TAGTTCCTTT GATGGAGATT TTGGTACATT CACTGGACTG 
GAGCAAACAG AACCTGAGCC AGAACCGGAT GTGGGACCCA GTTATGTTAG AAGTAATCGA 
GCTTTGTATG GTCTTCAGCG TATTGACACA AGTGCATACA CTAGTTTTCC TCTTAGTCCT 
GATGACTTTG CTCCAGAACA AGATGTTCCT GTCGCCAGTG ATAAAGACAA ACCTGTTGAA 
GGTGCTGATC AATTTTCCTT CCATTCTGAT CACGATGATG CAGTCAGTGA AGTGAAGCCT 
GAGGTTGAAG AAGCTCTTTC TCCTGAGGGA AATCCTGCTA GCCCAAGTCC TCTTGACCAA 
GAGTTTGACA AGCCCTGTGA TGACTACATT CAGGAAATTC CTGCAGTCCA TAGTCCAGAG 
CCTTCTGAAG TAGATGTTTA TGACCACAAA TTTGGAGATA TATATTTCTT TACAGTGCGG 
TTAGACCCTG GTAGTCCAGA TGCTAGCCCT GGTTCTGCCG AAGCCAATGA GCAAAAGAAA 
TCTGAATGTG ACCAAACAAG TCCTGAATCT AATGAAGCGG ATGAATTTGA CTCTGCTGCT 
CCAGAAGAAG AGGATACTAA TCACTATAAT CCTGAAGCAT ATTTGGAGGA AAGTTTTGAC 
AAAGAAGATT CTTCGATCAG AACAGAAGAA ACCCTGAGTC CTGAACCAAA TGATCCAGAA 
CAGTCTGAAC CTGAGGTAAG CCCCACAGGA TACAGCCCAA ATTTTGAGCA GGAAGATGAG 
GATAATGGTC TTGATGAGTT TGGACATAGT ATATTTAATG TAGAGAGCAG CAGTGTACTG 
AAAAACACTG AAAATGAAAT ACCTGAATCT TTTCACCCCG AAAATGTTGA CTCTGAAAAT 
GAAATACCTG TAAAAGTTGA CCCTGCCTAT ACTGAACACT ATTCAGTCCA CTTTACCACT 
GAACCTGTTG ATCAGTTTCT CATCAGCCCT GTCTCTGGAG ACACAGAAAA GGCAGCTTCT 
AACAGTTCCA AGCTTTGTTC TCATAACCCA TTCAGCCTTG ACTCTGAAAG CATAGATCAC 
TGTGATGACG CTGAAAGCAA TCTTCAACCT TTTAACACCA AAAACTGCAC TGGAAAAGAG 
TCTTATGGCT GTGTCAATGA GCTTAAATCT TTTGACCCAT TCGATCATGT ACTTATCGAA 
AACACAATAT TAGTTAAATC GGCCAATTCT ACATGTACTG ACAGTGGAAG ACTAGATTCC 
GGCTATCATG AAAATAGTGG GCTGGATCCT TTCAGTCCTG CACCTTCACA TCCTTCTACA 
GACATGGGTC CATGCTTTGT GAGTTCCAAC ACAGAGATGC TCAGTCCTGG TCTTTTGGAC 
CTGGAGGCTC CTGATCCCTT CAGCCCTGAG TCCATTGGGT CCAGTAGTAC AATGAGATTT 
CACTCTGAAC TCTTCAACCC TTCCACCGAC GATGGAATGA TTGGAGATCC ATGTCAGGTA 
TATACAGCCT CGTCCGACAT CCTTGGGGAT TCCTTTAGTG GGTCAGAACT TGACAGTTTA 
GACCCTTTCA GTCCGGTGCC ACACTTGACA GGGGGAAGTT ACAGTCATTG CAATGTAGAC 
ATTCTTGTCC CTGGCTTGAT TCAGAACAAT TCAAAATTTG ATGGAGAAAC TAGATCCAGC 
AGCTGTGATT CAGGGGTTGG TTCTGCTCAA AATAGAGAAG GTTCCCAGCC ACCAATCCAC 
TCTGAGGTCA ATAACTTTGT TTATTTGCCC AATTGCCCAA CAAAGGACTC TTTTTTTGAG 
TCGGATAACA GTAACATGGT TACAGATGAT CATGCTTTCC AATCAAAAGT GGAGAACGTA 
TCAGAACAGG AACAGAACAC AAATTCTTCA GCAAATGACA CATCGATGGC AGAACTCACC 
GAAATAGATT TTTTCTGCAA TGAGCTCAGC AAAATGGTGG CCTCACGTTC CTCTGATACT 
GTTCAGCAAA GTGTTGCTGA AATGCTGTTC GGCTCTAACC CTGACACCGC AAGCTTCTAC 
CCATGGGATT TCGAGAACAG CGACACAGTT GCCAACAATT ACTCAAGTCC AGAAGCCGAT 
TTAGTGGACA ACAGCTACAA CTACAGCAAG GATCTGCCGA AGTTGGAGTC AGGAACCAGA 
TCACGTAGGT CCAGTGACCT TCTAGGTGAA CTTCAGAGCG CTGCTGGACT TGCACATGAT 
GAAACTGAAA AGCCCTGCTT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007254679.1
CDS	1..3627
Translation	MRENSVELQTKDKDAANDNEIKVLEDVVAMEAGTLQKERVLSQDAQQALERLTKRRTQLKEEIEGIQEQRKQEGQLLLSLQEEQEELEQMVQEYEVELERANDELQHLHEEVRKTRARVEDAHGRMGPLKHSIGETYTEITETKERLGELIAEMIAIEACVPEITCHQMAEHLSIDLDNSSREEEDDDDEECYGHAHEDENTIKKITVEKYHSEQEQNIEGSETLQRSAENLKSSGSSSFTEITLTEVKEEDVSPRSNTPQHMDSPDLEEDFEIVQSVDTKPINQTSSREFDFFHPDPFVDHDVFGDDRFPKVDMTEFLSGDPFKGTDPFASDILFNDVADAQFSQDYPHNEESNTNKDEHADAANAVIHSSANADCQSIDSGMFEQNIPEPSDSERYTPYSDVCPDVGSNNTVSQELDSMELNNTSGTEFDLPASSQLDDEGRFDPIHCELHDSHESDSSESSTVDSSFDGDFGTFTGLEQTEPEPEPDVGPSYVRSNRALYGLQRIDTSAYTSFPLSPDDFAPEQDVPVASDKDKPVEGADQFSFHSDHDDAVSEVKPEVEEALSPEGNPASPSPLDQEFDKPCDDYIQEIPAVHSPEPSEVDVYDHKFGDIYFFTVRLDPGSPDASPGSAEANEQKKSECDQTSPESNEADEFDSAAPEEEDTNHYNPEAYLEESFDKEDSSIRTEETLSPEPNDPEQSEPEVSPTGYSPNFEQEDEDNGLDEFGHSIFNVESSSVLKNTENEIPESFHPENVDSENEIPVKVDPAYTEHYSVHFTTEPVDQFLISPVSGDTEKAASNSSKLCSHNPFSLDSESIDHCDDAESNLQPFNTKNCTGKESYGCVNELKSFDPFDHVLIENTILVKSANSTCTDSGRLDSGYHENSGLDPFSPAPSHPSTDMGPCFVSSNTEMLSPGLLDLEAPDPFSPESIGSSSTMRFHSELFNPSTDDGMIGDPCQVYTASSDILGDSFSGSELDSLDPFSPVPHLTGGSYSHCNVDILVPGLIQNNSKFDGETRSSSCDSGVGSAQNREGSQPPIHSEVNNFVYLPNCPTKDSFFESDNSNMVTDDHAFQSKVENVSEQEQNTNSSANDTSMAELTEIDFFCNELSKMVASRSSDTVQQSVAEMLFGSNPDTASFYPWDFENSDTVANNYSSPEADLVDNSYNYSKDLPKLESGTRSRRSSDLLGELQSAAGLAHDETEKPCLL

Target ORF information:

RefSeq Version	XM_007254617.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus dentin sialophosphoprotein-like (LOC103028904), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007254617.2

ORF Insert Sequence:

ATGAGAGAAA ACTCAGTGGA ACTGCAAACG AAGGACAAAG ATGCTGCCAA CGATAATGAA 
ATCAAGGTAC TGGAGGATGT GGTGGCTATG GAAGCAGGAA CACTGCAGAA GGAGAGGGTG 
CTGTCCCAGG ATGCCCAGCA GGCCCTGGAG AGACTTACGA AAAGGAGAAC CCAGCTGAAG 
GAGGAAATAG AGGGCATTCA GGAGCAGCGC AAACAGGAGG GCCAGCTGCT TCTGTCACTG 
CAGGAGGAGC AGGAGGAGCT GGAGCAGATG GTGCAGGAGT ACGAGGTGGA GCTGGAGAGA 
GCTAACGATG AGCTACAGCA CCTCCATGAG GAGGTCAGGA AGACCAGAGC AAGGGTGGAA 
GATGCACATG GTCGAATGGG TCCACTCAAA CACTCCATCG GGGAAACATA CACAGAAATC 
ACTGAGACTA AAGAGAGATT AGGTGAACTG ATTGCTGAGA TGATTGCCAT TGAAGCTTGT 
GTTCCTGAGA TTACCTGCCA TCAGATGGCT GAGCACTTGA GCATAGACCT TGATAACAGT 
TCAAGAGAAG AAGAAGATGA TGATGATGAA GAGTGCTATG GGCATGCACA TGAGGATGAA 
AACACAATCA AGAAAATCAC TGTGGAGAAA TATCATTCTG AGCAGGAACA AAACATAGAG 
GGATCTGAAA CACTGCAGCG TTCTGCAGAA AACTTGAAGA GCAGTGGTTC TTCTTCGTTT 
ACTGAGATTA CATTAACTGA AGTTAAAGAA GAGGACGTGT CCCCCAGATC TAACACACCA 
CAGCACATGG ACTCTCCTGA TTTGGAAGAA GATTTTGAGA TAGTCCAGTC TGTCGACACA 
AAGCCAATTA ACCAGACCTC AAGCAGGGAG TTTGACTTCT TCCACCCAGA CCCTTTTGTT 
GATCATGATG TGTTTGGAGA TGACAGATTC CCAAAAGTAG ACATGACAGA GTTCCTGTCT 
GGTGATCCAT TCAAAGGCAC AGATCCTTTT GCTTCCGATA TCCTTTTCAA CGATGTAGCA 
GATGCCCAGT TCTCTCAGGA TTACCCTCAC AATGAAGAAA GCAACACCAA TAAAGATGAA 
CATGCTGATG CAGCTAATGC TGTTATACAT AGTTCTGCCA ATGCTGACTG TCAGTCCATT 
GACTCTGGAA TGTTTGAACA AAATATTCCT GAGCCCAGTG ACTCTGAAAG GTACACTCCG 
TACAGCGACG TCTGTCCAGA TGTTGGATCC AACAACACAG TAAGCCAAGA GCTTGACAGC 
ATGGAACTAA ATAACACCTC AGGAACCGAA TTCGATCTTC CTGCAAGTTC CCAGCTTGAC 
GATGAAGGGC GATTTGACCC GATCCATTGT GAACTTCACG ACAGCCATGA ATCTGATTCC 
AGTGAATCTT CCACAGTTGA TAGTTCCTTT GATGGAGATT TTGGTACATT CACTGGACTG 
GAGCAAACAG AACCTGAGCC AGAACCGGAT GTGGGACCCA GTTATGTTAG AAGTAATCGA 
GCTTTGTATG GTCTTCAGCG TATTGACACA AGTGCATACA CTAGTTTTCC TCTTAGTCCT 
GATGACTTTG CTCCAGAACA AGATGTTCCT GTCGCCAGTG ATAAAGACAA ACCTGTTGAA 
GGTGCTGATC AATTTTCCTT CCATTCTGAT CACGATGATG CAGTCAGTGA AGTGAAGCCT 
GAGGTTGAAG AAGCTCTTTC TCCTGAGGGA AATCCTGCTA GCCCAAGTCC TCTTGACCAA 
GAGTTTGACA AGCCCTGTGA TGACTACATT CAGGAAATTC CTGCAGTCCA TAGTCCAGAG 
CCTTCTGAAG TAGATGTTTA TGACCACAAA TTTGGAGATA TATATTTCTT TACAGTGCGG 
TTAGACCCTG GTAGTCCAGA TGCTAGCCCT GGTTCTGCCG AAGCCAATGA GCAAAAGAAA 
TCTGAATGTG ACCAAACAAG TCCTGAATCT AATGAAGCGG ATGAATTTGA CTCTGCTGCT 
CCAGAAGAAG AGGATACTAA TCACTATAAT CCTGAAGCAT ATTTGGAGGA AAGTTTTGAC 
AAAGAAGATT CTTCGATCAG AACAGAAGAA ACCCTGAGTC CTGAACCAAA TGATCCAGAA 
CAGTCTGAAC CTGAGGTAAG CCCCACAGGA TACAGCCCAA ATTTTGAGCA GGAAGATGAG 
GATAATGGTC TTGATGAGTT TGGACATAGT ATATTTAATG TAGAGAGCAG CAGTGTACTG 
AAAAACACTG AAAATGAAAT ACCTGAATCT TTTCACCCCG AAAATGTTGA CTCTGAAAAT 
GAAATACCTG TAAAAGTTGA CCCTGCCTAT ACTGAACACT ATTCAGTCCA CTTTACCACT 
GAACCTGTTG ATCAGTTTCT CATCAGCCCT GTCTCTGGAG ACACAGAAAA GGCAGCTTCT 
AACAGTTCCA AGCTTTGTTC TCATAACCCA TTCAGCCTTG ACTCTGAAAG CATAGATCAC 
TGTGATGACG CTGAAAGCAA TCTTCAACCT TTTAACACCA AAAACTGCAC TGGAAAAGAG 
TCTTATGGCT GTGTCAATGA GCTTAAATCT TTTGACCCAT TCGATCATGT ACTTATCGAA 
AACACAATAT TAGTTAAATC GGCCAATTCT ACATGTACTG ACAGTGGAAG ACTAGATTCC 
GGCTATCATG AAAATAGTGG GCTGGATCCT TTCAGTCCTG CACCTTCACA TCCTTCTACA 
GACATGGGTC CATGCTTTGT GAGTTCCAAC ACAGAGATGC TCAGTCCTGG TCTTTTGGAC 
CTGGAGGCTC CTGATCCCTT CAGCCCTGAG TCCATTGGGT CCAGTAGTAC AATGAGATTT 
CACTCTGAAC TCTTCAACCC TTCCACCGAC GATGGAATGA TTGGAGATCC ATGTCAGGTA 
TATACAGCCT CGTCCGACAT CCTTGGGGAT TCCTTTAGTG GGTCAGAACT TGACAGTTTA 
GACCCTTTCA GTCCGGTGCC ACACTTGACA GGGGGAAGTT ACAGTCATTG CAATGTAGAC 
ATTCTTGTCC CTGGCTTGAT TCAGAACAAT TCAAAATTTG ATGGAGAAAC TAGATCCAGC 
AGCTGTGATT CAGGGGTTGG TTCTGCTCAA AATAGAGAAG GTTCCCAGCC ACCAATCCAC 
TCTGAGGTCA ATAACTTTGT TTATTTGCCC AATTGCCCAA CAAAGGACTC TTTTTTTGAG 
TCGGATAACA GTAACATGGT TACAGATGAT CATGCTTTCC AATCAAAAGT GGAGAACGTA 
TCAGAACAGG AACAGAACAC AAATTCTTCA GCAAATGACA CATCGATGGC AGAACTCACC 
GAAATAGATT TTTTCTGCAA TGAGCTCAGC AAAATGGTGG CCTCACGTTC CTCTGATACT 
GTTCAGCAAA GTGTTGCTGA AATGCTGTTC GGCTCTAACC CTGACACCGC AAGCTTCTAC 
CCATGGGATT TCGAGAACAG CGACACAGTT GCCAACAATT ACTCAAGTCC AGAAGCCGAT 
TTAGTGGACA ACAGCTACAA CTACAGCAAG GATCTGCCGA AGTTGGAGTC AGGAACCAGA 
TCACGTAGGT CCAGTGACCT TCTAGGTGAA CTTCAGAGCG CTGCTGGACT TGCACATGAT 
GAAACTGAAA AGCCCTGCTT GCTTTGA