tbc1d4 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d4
Entrez Gene ID	101064612
Full Name	TBC1 domain family member 4
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011606853.1	XP_011605155.1	TBC1 domain family member 4 isoform X4
XM_011606840.1	XP_011605142.1	TBC1 domain family member 4 isoform X2
XM_011606846.1	XP_011605148.1	TBC1 domain family member 4 isoform X3
XM_011606840.2	XP_011605142.2	TBC1 domain family member 4 isoform X2
XM_011606853.2	XP_011605155.2	TBC1 domain family member 4 isoform X4
XM_011606835.1	XP_011605137.1	TBC1 domain family member 4 isoform X1
XM_011606835.2	XP_011605137.2	TBC1 domain family member 4 isoform X1
XM_011606846.2	XP_011605148.2	TBC1 domain family member 4 isoform X3

Pathways from BioSystems
Gene Ontology

The following tbc1d4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo42421	XM_011606835.2 Latest version!	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo42422	XM_011606840.2 Latest version!	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo42423	XM_011606846.2 Latest version!	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo42424	XM_011606853.2 Latest version!	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo21264	XM_011606835.1 Latest version!	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo21265	XM_011606840.1 Latest version!	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo21266	XM_011606846.1 Latest version!	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo21267	XM_011606853.1 Latest version!	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d4 ( XM_011606835.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605137.2 Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo42421
Clone ID Related Accession (Same CDS sequence)	XM_011606835.2
Accession Version	XM_011606835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	TBC1 domain family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGAAGCGAG CCGTGATGAA CTGCACATAC GCAAGATGAA ACTGAACTAT 
CAGGAAGTGG GCCAGTGCTC CAAAGATGCT CAGGCACTGT GGGAGAGAAA ACTAACAGCC 
CCAGGCAGAA CAACTGTTCA GCAAGATAAG AAGGAGTTAT ACCATGCGCT CTGCCAAGGT 
GTTCCCAAGA ACAGACGTGG GGAAGTTTGG TTGCTTCTTT CCTATCAGCA TCGGCTCCGC 
CACAGGCTGC CCCAGCGTCT GCATGCCCCA GACACACCCT ATCAGGATTT GCTGAAGCAG 
CTGACCGCGC AGCAACATGC CATTCTGGTG GATCTAGGGC GGACCTTCCC CACCCACCAA 
TACTTCTCAG CCCAGCTGGG TGCAGGGCAA CTTTCCCTCT ACAACCTCCT TAAAGCTTAT 
TCTCTGCTGG ATACAGAGGT TGGTTACTGT CAGGGTATCA GCTTTGTAGC CGGAGTGCTG 
CTGCTTCACA TGAGCGAGGA GCAGGCCTTT GACATGCTGA GGTTCCTCAT GTATGACCTG 
GGCATCAGGC AGCAGTACAA ACCAGACATG ATCTCTCTGC AGATTCAGAT GTACCAGCTC 
TCCAGACTGC TCCATGACTA CCACCGTGAG TTGTACACTC ATCTCGAGGA TCACGAGATC 
AGCCCAAGCC TTTACGCAGC ACCGTGGTTC CTCACACTCT TCGCCTCACA GTTCCCACTC 
GGCTTTGTGT CGCGTATTTT TGATTTTGTA TTCGTCCAAG GGACTGGAGT GATATTTAAA 
GTGGCACTCT GTCTGCTGAG CAGCCATGAG GGGGAGATAA TGGAATGTGA CAGCTTTGAG 
AGCATTGTGG ACTATTTAAA ATCTACACTT CCTGCCCTCA GTCAGTCACA GATGGAAAAG 
ACTATTGCAA AGGTCGTGGA TATGGACATC TCCAAGCAAC TGCACGCATA TGAAGTGGAG 
TACCATGTCC TGCAGGATGA GATGCTGGAT GCAGGTGCTG TGTTGGATGA CTCTGACCGT 
CTTGACAAGC TGGAAAAGAC CAACATGCAG CTGAAAAGGC AAAACATGGA CCTGCTGGAA 
AAACTTCAGG CTGCGAGGCA GAAGATTCAG TCGCTGGAGA CGAGTGTGGA GAACTTCCTT 
TCTCGGGAGA GCAAAATGAA GCACATGATT CGCTCCTTGG AGCAGGAGAA GGCAGCTTAC 
CAGAAGACCA TTGAACGCAT GCGCTCATGC CTTCCCCTTG ACACTCCGAC AGATGTGGAG 
ATGACCCAGA TCAAAACAGG TCCCAACGGG AAAGCCAAAA CTGAAGCCAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605137.2
CDS	649..4368
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERQDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSPTAVSSRTRSVSESESSFNLPSSFSAPTFLKSIYQGSLGSLSSLADSGTLKSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEKTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606835.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606835.2

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGAAGCGAG CCGTGATGAA CTGCACATAC GCAAGATGAA ACTGAACTAT 
CAGGAAGTGG GCCAGTGCTC CAAAGATGCT CAGGCACTGT GGGAGAGAAA ACTAACAGCC 
CCAGGCAGAA CAACTGTTCA GCAAGATAAG AAGGAGTTAT ACCATGCGCT CTGCCAAGGT 
GTTCCCAAGA ACAGACGTGG GGAAGTTTGG TTGCTTCTTT CCTATCAGCA TCGGCTCCGC 
CACAGGCTGC CCCAGCGTCT GCATGCCCCA GACACACCCT ATCAGGATTT GCTGAAGCAG 
CTGACCGCGC AGCAACATGC CATTCTGGTG GATCTAGGGC GGACCTTCCC CACCCACCAA 
TACTTCTCAG CCCAGCTGGG TGCAGGGCAA CTTTCCCTCT ACAACCTCCT TAAAGCTTAT 
TCTCTGCTGG ATACAGAGGT TGGTTACTGT CAGGGTATCA GCTTTGTAGC CGGAGTGCTG 
CTGCTTCACA TGAGCGAGGA GCAGGCCTTT GACATGCTGA GGTTCCTCAT GTATGACCTG 
GGCATCAGGC AGCAGTACAA ACCAGACATG ATCTCTCTGC AGATTCAGAT GTACCAGCTC 
TCCAGACTGC TCCATGACTA CCACCGTGAG TTGTACACTC ATCTCGAGGA TCACGAGATC 
AGCCCAAGCC TTTACGCAGC ACCGTGGTTC CTCACACTCT TCGCCTCACA GTTCCCACTC 
GGCTTTGTGT CGCGTATTTT TGATTTTGTA TTCGTCCAAG GGACTGGAGT GATATTTAAA 
GTGGCACTCT GTCTGCTGAG CAGCCATGAG GGGGAGATAA TGGAATGTGA CAGCTTTGAG 
AGCATTGTGG ACTATTTAAA ATCTACACTT CCTGCCCTCA GTCAGTCACA GATGGAAAAG 
ACTATTGCAA AGGTCGTGGA TATGGACATC TCCAAGCAAC TGCACGCATA TGAAGTGGAG 
TACCATGTCC TGCAGGATGA GATGCTGGAT GCAGGTGCTG TGTTGGATGA CTCTGACCGT 
CTTGACAAGC TGGAAAAGAC CAACATGCAG CTGAAAAGGC AAAACATGGA CCTGCTGGAA 
AAACTTCAGG CTGCGAGGCA GAAGATTCAG TCGCTGGAGA CGAGTGTGGA GAACTTCCTT 
TCTCGGGAGA GCAAAATGAA GCACATGATT CGCTCCTTGG AGCAGGAGAA GGCAGCTTAC 
CAGAAGACCA TTGAACGCAT GCGCTCATGC CTTCCCCTTG ACACTCCGAC AGATGTGGAG 
ATGACCCAGA TCAAAACAGG TCCCAACGGG AAAGCCAAAA CTGAAGCCAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606840.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605142.2 Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo42422
Clone ID Related Accession (Same CDS sequence)	XM_011606840.2
Accession Version	XM_011606840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	TBC1 domain family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAAAAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605142.2
CDS	648..4364
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERQDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSPTAVSSRTRSVSESESSFNLPSSFSAPTFLKSIYQGSLGSLSSLADSGTLKSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEKTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606840.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606840.2

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAAAAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606846.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605148.2 Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X3, mRNA.

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CloneID	OTo42423
Clone ID Related Accession (Same CDS sequence)	XM_011606846.2
Accession Version	XM_011606846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	TBC1 domain family member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGG ATGAGGAATC TCCGGGGCCC TCCCCTCCAG GTTCTCCCCC CACCTTTGCT 
GAAAATGACC CGGAAAATGG CGTCCAGTTC CGCCGGCGTG CCCACACCTT CAGCCACCCA 
CCTGTGAAGA AACGCATCTC CTTCGAGGGC CAGTCAAACA GCCAGAGCAA GGCTGCCCTG 
CACAGGCAAC AGTCTGTAAA CCCCGAGCTG CTCCAGAGCA GTCCCACGGC TGTCTCCTCA 
AGAACGCGCA GCGTTTCGGA GAGCGAGTCC TCCTTCAACC TCCCCTCCTC TTTCTCCGCT 
CCCACTTTCC TGAAAAGCAT TTACCAGGGT TCACTGGGCT CCCTGAGCTC CCTGGCAGAC 
AGTGGGACCC TCAAGAGCAA CGGTGAAGGC AGGAAGAGAA CCCTTTCTAG CTGCAGTAAT 
GACTCGCTGA GCTGGGGGCT GCCTCCGACC CCACGCAGGG TCTCCTGGAG ACAGAAGATC 
TTCCTCAGGG TTGCATCACC GATGAGCAAA TCACCTGCAC CCATGCACCA CCCAGACGAC 
TCTAAAGGCC AGGAGTTGTT GCCTCTTTCT CCACATTCAA GAAGATCTGT TATGGACCCA 
TCAAGGCATC TGCTTCCACC GGGGGAGAAG CCCAAGAGGA CCTCAGCTGA CTATCGGGCC 
CTATGGAAAA CTGCCATCCA CCAGCAGATC CTACTTCTGC GCATGGAAAA GGAGAACCAG 
AGACTGGAAG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAAAAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605148.2
CDS	651..4367
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSPTAVSSRTRSVSESESSFNLPSSFSAPTFLKSIYQGSLGSLSSLADSGTLKSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEKTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606846.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606846.2

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGG ATGAGGAATC TCCGGGGCCC TCCCCTCCAG GTTCTCCCCC CACCTTTGCT 
GAAAATGACC CGGAAAATGG CGTCCAGTTC CGCCGGCGTG CCCACACCTT CAGCCACCCA 
CCTGTGAAGA AACGCATCTC CTTCGAGGGC CAGTCAAACA GCCAGAGCAA GGCTGCCCTG 
CACAGGCAAC AGTCTGTAAA CCCCGAGCTG CTCCAGAGCA GTCCCACGGC TGTCTCCTCA 
AGAACGCGCA GCGTTTCGGA GAGCGAGTCC TCCTTCAACC TCCCCTCCTC TTTCTCCGCT 
CCCACTTTCC TGAAAAGCAT TTACCAGGGT TCACTGGGCT CCCTGAGCTC CCTGGCAGAC 
AGTGGGACCC TCAAGAGCAA CGGTGAAGGC AGGAAGAGAA CCCTTTCTAG CTGCAGTAAT 
GACTCGCTGA GCTGGGGGCT GCCTCCGACC CCACGCAGGG TCTCCTGGAG ACAGAAGATC 
TTCCTCAGGG TTGCATCACC GATGAGCAAA TCACCTGCAC CCATGCACCA CCCAGACGAC 
TCTAAAGGCC AGGAGTTGTT GCCTCTTTCT CCACATTCAA GAAGATCTGT TATGGACCCA 
TCAAGGCATC TGCTTCCACC GGGGGAGAAG CCCAAGAGGA CCTCAGCTGA CTATCGGGCC 
CTATGGAAAA CTGCCATCCA CCAGCAGATC CTACTTCTGC GCATGGAAAA GGAGAACCAG 
AGACTGGAAG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAAAAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606853.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605155.2 Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X4, mRNA.

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CloneID	OTo42424
Clone ID Related Accession (Same CDS sequence)	XM_011606853.2
Accession Version	XM_011606853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	TBC1 domain family member 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGCAACGG TGAAGGCAGG 
AAGAGAACCC TTTCTAGCTG CAGTAATGAC TCGCTGAGCT GGGGGCTGCC TCCGACCCCA 
CGCAGGGTCT CCTGGAGACA GAAGATCTTC CTCAGGGTTG CATCACCGAT GAGCAAATCA 
CCTGCACCCA TGCACCACCC AGACGACTCT AAAGGCCAGG AGTTGTTGCC TCTTTCTCCA 
CATTCAAGAA GATCTGTTAT GGACCCATCA AGGCATCTGC TTCCACCGGG GGAGAAGCCC 
AAGAGGACCT CAGCTGACTA TCGGGCCCTA TGGAAAACTG CCATCCACCA GCAGATCCTA 
CTTCTGCGCA TGGAAAAGGA GAACCAGAGA CTGGAAGAAG CGAGCCGTGA TGAACTGCAC 
ATACGCAAGA TGAAACTGAA CTATCAGGAA GTGGGCCAGT GCTCCAAAGA TGCTCAGGCA 
CTGTGGGAGA GAAAACTAAC AGCCCCAGGC AGAACAACTG TTCAGCAAGA TAAGAAGGAG 
TTATACCATG CGCTCTGCCA AGGTGTTCCC AAGAACAGAC GTGGGGAAGT TTGGTTGCTT 
CTTTCCTATC AGCATCGGCT CCGCCACAGG CTGCCCCAGC GTCTGCATGC CCCAGACACA 
CCCTATCAGG ATTTGCTGAA GCAGCTGACC GCGCAGCAAC ATGCCATTCT GGTGGATCTA 
GGGCGGACCT TCCCCACCCA CCAATACTTC TCAGCCCAGC TGGGTGCAGG GCAACTTTCC 
CTCTACAACC TCCTTAAAGC TTATTCTCTG CTGGATACAG AGGTTGGTTA CTGTCAGGGT 
ATCAGCTTTG TAGCCGGAGT GCTGCTGCTT CACATGAGCG AGGAGCAGGC CTTTGACATG 
CTGAGGTTCC TCATGTATGA CCTGGGCATC AGGCAGCAGT ACAAACCAGA CATGATCTCT 
CTGCAGATTC AGATGTACCA GCTCTCCAGA CTGCTCCATG ACTACCACCG TGAGTTGTAC 
ACTCATCTCG AGGATCACGA GATCAGCCCA AGCCTTTACG CAGCACCGTG GTTCCTCACA 
CTCTTCGCCT CACAGTTCCC ACTCGGCTTT GTGTCGCGTA TTTTTGATTT TGTATTCGTC 
CAAGGGACTG GAGTGATATT TAAAGTGGCA CTCTGTCTGC TGAGCAGCCA TGAGGGGGAG 
ATAATGGAAT GTGACAGCTT TGAGAGCATT GTGGACTATT TAAAATCTAC ACTTCCTGCC 
CTCAGTCAGT CACAGATGGA AAAGACTATT GCAAAGGTCG TGGATATGGA CATCTCCAAG 
CAACTGCACG CATATGAAGT GGAGTACCAT GTCCTGCAGG ATGAGATGCT GGATGCAGGT 
GCTGTGTTGG ATGACTCTGA CCGTCTTGAC AAGCTGGAAA AGACCAACAT GCAGCTGAAA 
AGGCAAAACA TGGACCTGCT GGAAAAACTT CAGGCTGCGA GGCAGAAGAT TCAGTCGCTG 
GAGACGAGTG TGGAGAACTT CCTTTCTCGG GAGAGCAAAA TGAAGCACAT GATTCGCTCC 
TTGGAGCAGG AGAAGGCAGC TTACCAGAAG ACCATTGAAC GCATGCGCTC ATGCCTTCCC 
CTTGACACTC CGACAGATGT GGAGATGACC CAGATCAAAA CAGGTCCCAA CGGGAAAGCC 
AAAACTGAAG CCAAGAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605155.2
CDS	650..4213
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERQDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEKTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606853.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes TBC1 domain family member 4 (tbc1d4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606853.2

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGCAACGG TGAAGGCAGG 
AAGAGAACCC TTTCTAGCTG CAGTAATGAC TCGCTGAGCT GGGGGCTGCC TCCGACCCCA 
CGCAGGGTCT CCTGGAGACA GAAGATCTTC CTCAGGGTTG CATCACCGAT GAGCAAATCA 
CCTGCACCCA TGCACCACCC AGACGACTCT AAAGGCCAGG AGTTGTTGCC TCTTTCTCCA 
CATTCAAGAA GATCTGTTAT GGACCCATCA AGGCATCTGC TTCCACCGGG GGAGAAGCCC 
AAGAGGACCT CAGCTGACTA TCGGGCCCTA TGGAAAACTG CCATCCACCA GCAGATCCTA 
CTTCTGCGCA TGGAAAAGGA GAACCAGAGA CTGGAAGAAG CGAGCCGTGA TGAACTGCAC 
ATACGCAAGA TGAAACTGAA CTATCAGGAA GTGGGCCAGT GCTCCAAAGA TGCTCAGGCA 
CTGTGGGAGA GAAAACTAAC AGCCCCAGGC AGAACAACTG TTCAGCAAGA TAAGAAGGAG 
TTATACCATG CGCTCTGCCA AGGTGTTCCC AAGAACAGAC GTGGGGAAGT TTGGTTGCTT 
CTTTCCTATC AGCATCGGCT CCGCCACAGG CTGCCCCAGC GTCTGCATGC CCCAGACACA 
CCCTATCAGG ATTTGCTGAA GCAGCTGACC GCGCAGCAAC ATGCCATTCT GGTGGATCTA 
GGGCGGACCT TCCCCACCCA CCAATACTTC TCAGCCCAGC TGGGTGCAGG GCAACTTTCC 
CTCTACAACC TCCTTAAAGC TTATTCTCTG CTGGATACAG AGGTTGGTTA CTGTCAGGGT 
ATCAGCTTTG TAGCCGGAGT GCTGCTGCTT CACATGAGCG AGGAGCAGGC CTTTGACATG 
CTGAGGTTCC TCATGTATGA CCTGGGCATC AGGCAGCAGT ACAAACCAGA CATGATCTCT 
CTGCAGATTC AGATGTACCA GCTCTCCAGA CTGCTCCATG ACTACCACCG TGAGTTGTAC 
ACTCATCTCG AGGATCACGA GATCAGCCCA AGCCTTTACG CAGCACCGTG GTTCCTCACA 
CTCTTCGCCT CACAGTTCCC ACTCGGCTTT GTGTCGCGTA TTTTTGATTT TGTATTCGTC 
CAAGGGACTG GAGTGATATT TAAAGTGGCA CTCTGTCTGC TGAGCAGCCA TGAGGGGGAG 
ATAATGGAAT GTGACAGCTT TGAGAGCATT GTGGACTATT TAAAATCTAC ACTTCCTGCC 
CTCAGTCAGT CACAGATGGA AAAGACTATT GCAAAGGTCG TGGATATGGA CATCTCCAAG 
CAACTGCACG CATATGAAGT GGAGTACCAT GTCCTGCAGG ATGAGATGCT GGATGCAGGT 
GCTGTGTTGG ATGACTCTGA CCGTCTTGAC AAGCTGGAAA AGACCAACAT GCAGCTGAAA 
AGGCAAAACA TGGACCTGCT GGAAAAACTT CAGGCTGCGA GGCAGAAGAT TCAGTCGCTG 
GAGACGAGTG TGGAGAACTT CCTTTCTCGG GAGAGCAAAA TGAAGCACAT GATTCGCTCC 
TTGGAGCAGG AGAAGGCAGC TTACCAGAAG ACCATTGAAC GCATGCGCTC ATGCCTTCCC 
CTTGACACTC CGACAGATGT GGAGATGACC CAGATCAAAA CAGGTCCCAA CGGGAAAGCC 
AAAACTGAAG CCAAGAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606835.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605137.1 Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X1, mRNA.

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CloneID	OTo21264
Clone ID Related Accession (Same CDS sequence)	XM_011606835.1
Accession Version	XM_011606835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	TBC1 domain family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGAAGCGAG CCGTGATGAA CTGCACATAC GCAAGATGAA ACTGAACTAT 
CAGGAAGTGG GCCAGTGCTC CAAAGATGCT CAGGCACTGT GGGAGAGAAA ACTAACAGCC 
CCAGGCAGAA CAACTGTTCA GCAAGATAAG AAGGAGTTAT ACCATGCGCT CTGCCAAGGT 
GTTCCCAAGA ACAGACGTGG GGAAGTTTGG TTGCTTCTTT CCTATCAGCA TCGGCTCCGC 
CACAGGCTGC CCCAGCGTCT GCATGCCCCA GACACACCCT ATCAGGATTT GCTGAAGCAG 
CTGACCGCGC AGCAACATGC CATTCTGGTG GATCTAGGGC GGACCTTCCC CACCCACCAA 
TACTTCTCAG CCCAGCTGGG TGCAGGGCAA CTTTCCCTCT ACAACCTCCT TAAAGCTTAT 
TCTCTGCTGG ATACAGAGGT TGGTTACTGT CAGGGTATCA GCTTTGTAGC CGGAGTGCTG 
CTGCTTCACA TGAGCGAGGA GCAGGCCTTT GACATGCTGA GGTTCCTCAT GTATGACCTG 
GGCATCAGGC AGCAGTACAA ACCAGACATG ATCTCTCTGC AGATTCAGAT GTACCAGCTC 
TCCAGACTGC TCCATGACTA CCACCGTGAG TTGTACACTC ATCTCGAGGA TCACGAGATC 
AGCCCAAGCC TTTACGCAGC ACCGTGGTTC CTCACACTCT TCGCCTCACA GTTCCCACTC 
GGCTTTGTGT CGCGTATTTT TGATTTTGTA TTCGTCCAAG GGACTGGAGT GATATTTAAA 
GTGGCACTCT GTCTGCTGAG CAGCCATGAG GGGGAGATAA TGGAATGTGA CAGCTTTGAG 
AGCATTGTGG ACTATTTAAA ATCTACACTT CCTGCCCTCA GTCAGTCACA GATGGAACAG 
ACTATTGCAA AGGTCGTGGA TATGGACATC TCCAAGCAAC TGCACGCATA TGAAGTGGAG 
TACCATGTCC TGCAGGATGA GATGCTGGAT GCAGGTGCTG TGTTGGATGA CTCTGACCGT 
CTTGACAAGC TGGAAAAGAC CAACATGCAG CTGAAAAGGC AAAACATGGA CCTGCTGGAA 
AAACTTCAGG CTGCGAGGCA GAAGATTCAG TCGCTGGAGA CGAGTGTGGA GAACTTCCTT 
TCTCGGGAGA GCAAAATGAA GCACATGATT CGCTCCTTGG AGCAGGAGAA GGCAGCTTAC 
CAGAAGACCA TTGAACGCAT GCGCTCATGC CTTCCCCTTG ACACTCCGAC AGATGTGGAG 
ATGACCCAGA TCAAAACAGG TCCCAACGGG AAAGCCAAAA CTGAAGCCAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605137.1
CDS	1906..5625
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERQDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSPTAVSSRTRSVSESESSFNLPSSFSAPTFLKSIYQGSLGSLSSLADSGTLKSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEQTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606835.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606835.1

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGAAGCGAG CCGTGATGAA CTGCACATAC GCAAGATGAA ACTGAACTAT 
CAGGAAGTGG GCCAGTGCTC CAAAGATGCT CAGGCACTGT GGGAGAGAAA ACTAACAGCC 
CCAGGCAGAA CAACTGTTCA GCAAGATAAG AAGGAGTTAT ACCATGCGCT CTGCCAAGGT 
GTTCCCAAGA ACAGACGTGG GGAAGTTTGG TTGCTTCTTT CCTATCAGCA TCGGCTCCGC 
CACAGGCTGC CCCAGCGTCT GCATGCCCCA GACACACCCT ATCAGGATTT GCTGAAGCAG 
CTGACCGCGC AGCAACATGC CATTCTGGTG GATCTAGGGC GGACCTTCCC CACCCACCAA 
TACTTCTCAG CCCAGCTGGG TGCAGGGCAA CTTTCCCTCT ACAACCTCCT TAAAGCTTAT 
TCTCTGCTGG ATACAGAGGT TGGTTACTGT CAGGGTATCA GCTTTGTAGC CGGAGTGCTG 
CTGCTTCACA TGAGCGAGGA GCAGGCCTTT GACATGCTGA GGTTCCTCAT GTATGACCTG 
GGCATCAGGC AGCAGTACAA ACCAGACATG ATCTCTCTGC AGATTCAGAT GTACCAGCTC 
TCCAGACTGC TCCATGACTA CCACCGTGAG TTGTACACTC ATCTCGAGGA TCACGAGATC 
AGCCCAAGCC TTTACGCAGC ACCGTGGTTC CTCACACTCT TCGCCTCACA GTTCCCACTC 
GGCTTTGTGT CGCGTATTTT TGATTTTGTA TTCGTCCAAG GGACTGGAGT GATATTTAAA 
GTGGCACTCT GTCTGCTGAG CAGCCATGAG GGGGAGATAA TGGAATGTGA CAGCTTTGAG 
AGCATTGTGG ACTATTTAAA ATCTACACTT CCTGCCCTCA GTCAGTCACA GATGGAACAG 
ACTATTGCAA AGGTCGTGGA TATGGACATC TCCAAGCAAC TGCACGCATA TGAAGTGGAG 
TACCATGTCC TGCAGGATGA GATGCTGGAT GCAGGTGCTG TGTTGGATGA CTCTGACCGT 
CTTGACAAGC TGGAAAAGAC CAACATGCAG CTGAAAAGGC AAAACATGGA CCTGCTGGAA 
AAACTTCAGG CTGCGAGGCA GAAGATTCAG TCGCTGGAGA CGAGTGTGGA GAACTTCCTT 
TCTCGGGAGA GCAAAATGAA GCACATGATT CGCTCCTTGG AGCAGGAGAA GGCAGCTTAC 
CAGAAGACCA TTGAACGCAT GCGCTCATGC CTTCCCCTTG ACACTCCGAC AGATGTGGAG 
ATGACCCAGA TCAAAACAGG TCCCAACGGG AAAGCCAAAA CTGAAGCCAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606840.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605142.1 Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X2, mRNA.

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CloneID	OTo21265
Clone ID Related Accession (Same CDS sequence)	XM_011606840.1
Accession Version	XM_011606840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	TBC1 domain family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAACAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605142.1
CDS	1906..5622
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERQDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSPTAVSSRTRSVSESESSFNLPSSFSAPTFLKSIYQGSLGSLSSLADSGTLKSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEQTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606840.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606840.1

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGTCCCAC GGCTGTCTCC 
TCAAGAACGC GCAGCGTTTC GGAGAGCGAG TCCTCCTTCA ACCTCCCCTC CTCTTTCTCC 
GCTCCCACTT TCCTGAAAAG CATTTACCAG GGTTCACTGG GCTCCCTGAG CTCCCTGGCA 
GACAGTGGGA CCCTCAAGAG CAACGGTGAA GGCAGGAAGA GAACCCTTTC TAGCTGCAGT 
AATGACTCGC TGAGCTGGGG GCTGCCTCCG ACCCCACGCA GGGTCTCCTG GAGACAGAAG 
ATCTTCCTCA GGGTTGCATC ACCGATGAGC AAATCACCTG CACCCATGCA CCACCCAGAC 
GACTCTAAAG GCCAGGAGTT GTTGCCTCTT TCTCCACATT CAAGAAGATC TGTTATGGAC 
CCATCAAGGC ATCTGCTTCC ACCGGGGGAG AAGCCCAAGA GGACCTCAGC TGACTATCGG 
GCCCTATGGA AAACTGCCAT CCACCAGCAG ATCCTACTTC TGCGCATGGA AAAGGAGAAC 
CAGAGACTGG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAACAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606846.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605148.1 Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo21266
Clone ID Related Accession (Same CDS sequence)	XM_011606846.1
Accession Version	XM_011606846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	TBC1 domain family member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGG ATGAGGAATC TCCGGGGCCC TCCCCTCCAG GTTCTCCCCC CACCTTTGCT 
GAAAATGACC CGGAAAATGG CGTCCAGTTC CGCCGGCGTG CCCACACCTT CAGCCACCCA 
CCTGTGAAGA AACGCATCTC CTTCGAGGGC CAGTCAAACA GCCAGAGCAA GGCTGCCCTG 
CACAGGCAAC AGTCTGTAAA CCCCGAGCTG CTCCAGAGCA GTCCCACGGC TGTCTCCTCA 
AGAACGCGCA GCGTTTCGGA GAGCGAGTCC TCCTTCAACC TCCCCTCCTC TTTCTCCGCT 
CCCACTTTCC TGAAAAGCAT TTACCAGGGT TCACTGGGCT CCCTGAGCTC CCTGGCAGAC 
AGTGGGACCC TCAAGAGCAA CGGTGAAGGC AGGAAGAGAA CCCTTTCTAG CTGCAGTAAT 
GACTCGCTGA GCTGGGGGCT GCCTCCGACC CCACGCAGGG TCTCCTGGAG ACAGAAGATC 
TTCCTCAGGG TTGCATCACC GATGAGCAAA TCACCTGCAC CCATGCACCA CCCAGACGAC 
TCTAAAGGCC AGGAGTTGTT GCCTCTTTCT CCACATTCAA GAAGATCTGT TATGGACCCA 
TCAAGGCATC TGCTTCCACC GGGGGAGAAG CCCAAGAGGA CCTCAGCTGA CTATCGGGCC 
CTATGGAAAA CTGCCATCCA CCAGCAGATC CTACTTCTGC GCATGGAAAA GGAGAACCAG 
AGACTGGAAG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAACAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605148.1
CDS	1906..5622
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSPTAVSSRTRSVSESESSFNLPSSFSAPTFLKSIYQGSLGSLSSLADSGTLKSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEQTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606846.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606846.1

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGG ATGAGGAATC TCCGGGGCCC TCCCCTCCAG GTTCTCCCCC CACCTTTGCT 
GAAAATGACC CGGAAAATGG CGTCCAGTTC CGCCGGCGTG CCCACACCTT CAGCCACCCA 
CCTGTGAAGA AACGCATCTC CTTCGAGGGC CAGTCAAACA GCCAGAGCAA GGCTGCCCTG 
CACAGGCAAC AGTCTGTAAA CCCCGAGCTG CTCCAGAGCA GTCCCACGGC TGTCTCCTCA 
AGAACGCGCA GCGTTTCGGA GAGCGAGTCC TCCTTCAACC TCCCCTCCTC TTTCTCCGCT 
CCCACTTTCC TGAAAAGCAT TTACCAGGGT TCACTGGGCT CCCTGAGCTC CCTGGCAGAC 
AGTGGGACCC TCAAGAGCAA CGGTGAAGGC AGGAAGAGAA CCCTTTCTAG CTGCAGTAAT 
GACTCGCTGA GCTGGGGGCT GCCTCCGACC CCACGCAGGG TCTCCTGGAG ACAGAAGATC 
TTCCTCAGGG TTGCATCACC GATGAGCAAA TCACCTGCAC CCATGCACCA CCCAGACGAC 
TCTAAAGGCC AGGAGTTGTT GCCTCTTTCT CCACATTCAA GAAGATCTGT TATGGACCCA 
TCAAGGCATC TGCTTCCACC GGGGGAGAAG CCCAAGAGGA CCTCAGCTGA CTATCGGGCC 
CTATGGAAAA CTGCCATCCA CCAGCAGATC CTACTTCTGC GCATGGAAAA GGAGAACCAG 
AGACTGGAAG AAGCGAGCCG TGATGAACTG CACATACGCA AGATGAAACT GAACTATCAG 
GAAGTGGGCC AGTGCTCCAA AGATGCTCAG GCACTGTGGG AGAGAAAACT AACAGCCCCA 
GGCAGAACAA CTGTTCAGCA AGATAAGAAG GAGTTATACC ATGCGCTCTG CCAAGGTGTT 
CCCAAGAACA GACGTGGGGA AGTTTGGTTG CTTCTTTCCT ATCAGCATCG GCTCCGCCAC 
AGGCTGCCCC AGCGTCTGCA TGCCCCAGAC ACACCCTATC AGGATTTGCT GAAGCAGCTG 
ACCGCGCAGC AACATGCCAT TCTGGTGGAT CTAGGGCGGA CCTTCCCCAC CCACCAATAC 
TTCTCAGCCC AGCTGGGTGC AGGGCAACTT TCCCTCTACA ACCTCCTTAA AGCTTATTCT 
CTGCTGGATA CAGAGGTTGG TTACTGTCAG GGTATCAGCT TTGTAGCCGG AGTGCTGCTG 
CTTCACATGA GCGAGGAGCA GGCCTTTGAC ATGCTGAGGT TCCTCATGTA TGACCTGGGC 
ATCAGGCAGC AGTACAAACC AGACATGATC TCTCTGCAGA TTCAGATGTA CCAGCTCTCC 
AGACTGCTCC ATGACTACCA CCGTGAGTTG TACACTCATC TCGAGGATCA CGAGATCAGC 
CCAAGCCTTT ACGCAGCACC GTGGTTCCTC ACACTCTTCG CCTCACAGTT CCCACTCGGC 
TTTGTGTCGC GTATTTTTGA TTTTGTATTC GTCCAAGGGA CTGGAGTGAT ATTTAAAGTG 
GCACTCTGTC TGCTGAGCAG CCATGAGGGG GAGATAATGG AATGTGACAG CTTTGAGAGC 
ATTGTGGACT ATTTAAAATC TACACTTCCT GCCCTCAGTC AGTCACAGAT GGAACAGACT 
ATTGCAAAGG TCGTGGATAT GGACATCTCC AAGCAACTGC ACGCATATGA AGTGGAGTAC 
CATGTCCTGC AGGATGAGAT GCTGGATGCA GGTGCTGTGT TGGATGACTC TGACCGTCTT 
GACAAGCTGG AAAAGACCAA CATGCAGCTG AAAAGGCAAA ACATGGACCT GCTGGAAAAA 
CTTCAGGCTG CGAGGCAGAA GATTCAGTCG CTGGAGACGA GTGTGGAGAA CTTCCTTTCT 
CGGGAGAGCA AAATGAAGCA CATGATTCGC TCCTTGGAGC AGGAGAAGGC AGCTTACCAG 
AAGACCATTG AACGCATGCG CTCATGCCTT CCCCTTGACA CTCCGACAGA TGTGGAGATG 
ACCCAGATCA AAACAGGTCC CAACGGGAAA GCCAAAACTG AAGCCAAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_011606853.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605155.1 Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo21267
Clone ID Related Accession (Same CDS sequence)	XM_011606853.1
Accession Version	XM_011606853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	TBC1 domain family member 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGCAACGG TGAAGGCAGG 
AAGAGAACCC TTTCTAGCTG CAGTAATGAC TCGCTGAGCT GGGGGCTGCC TCCGACCCCA 
CGCAGGGTCT CCTGGAGACA GAAGATCTTC CTCAGGGTTG CATCACCGAT GAGCAAATCA 
CCTGCACCCA TGCACCACCC AGACGACTCT AAAGGCCAGG AGTTGTTGCC TCTTTCTCCA 
CATTCAAGAA GATCTGTTAT GGACCCATCA AGGCATCTGC TTCCACCGGG GGAGAAGCCC 
AAGAGGACCT CAGCTGACTA TCGGGCCCTA TGGAAAACTG CCATCCACCA GCAGATCCTA 
CTTCTGCGCA TGGAAAAGGA GAACCAGAGA CTGGAAGAAG CGAGCCGTGA TGAACTGCAC 
ATACGCAAGA TGAAACTGAA CTATCAGGAA GTGGGCCAGT GCTCCAAAGA TGCTCAGGCA 
CTGTGGGAGA GAAAACTAAC AGCCCCAGGC AGAACAACTG TTCAGCAAGA TAAGAAGGAG 
TTATACCATG CGCTCTGCCA AGGTGTTCCC AAGAACAGAC GTGGGGAAGT TTGGTTGCTT 
CTTTCCTATC AGCATCGGCT CCGCCACAGG CTGCCCCAGC GTCTGCATGC CCCAGACACA 
CCCTATCAGG ATTTGCTGAA GCAGCTGACC GCGCAGCAAC ATGCCATTCT GGTGGATCTA 
GGGCGGACCT TCCCCACCCA CCAATACTTC TCAGCCCAGC TGGGTGCAGG GCAACTTTCC 
CTCTACAACC TCCTTAAAGC TTATTCTCTG CTGGATACAG AGGTTGGTTA CTGTCAGGGT 
ATCAGCTTTG TAGCCGGAGT GCTGCTGCTT CACATGAGCG AGGAGCAGGC CTTTGACATG 
CTGAGGTTCC TCATGTATGA CCTGGGCATC AGGCAGCAGT ACAAACCAGA CATGATCTCT 
CTGCAGATTC AGATGTACCA GCTCTCCAGA CTGCTCCATG ACTACCACCG TGAGTTGTAC 
ACTCATCTCG AGGATCACGA GATCAGCCCA AGCCTTTACG CAGCACCGTG GTTCCTCACA 
CTCTTCGCCT CACAGTTCCC ACTCGGCTTT GTGTCGCGTA TTTTTGATTT TGTATTCGTC 
CAAGGGACTG GAGTGATATT TAAAGTGGCA CTCTGTCTGC TGAGCAGCCA TGAGGGGGAG 
ATAATGGAAT GTGACAGCTT TGAGAGCATT GTGGACTATT TAAAATCTAC ACTTCCTGCC 
CTCAGTCAGT CACAGATGGA ACAGACTATT GCAAAGGTCG TGGATATGGA CATCTCCAAG 
CAACTGCACG CATATGAAGT GGAGTACCAT GTCCTGCAGG ATGAGATGCT GGATGCAGGT 
GCTGTGTTGG ATGACTCTGA CCGTCTTGAC AAGCTGGAAA AGACCAACAT GCAGCTGAAA 
AGGCAAAACA TGGACCTGCT GGAAAAACTT CAGGCTGCGA GGCAGAAGAT TCAGTCGCTG 
GAGACGAGTG TGGAGAACTT CCTTTCTCGG GAGAGCAAAA TGAAGCACAT GATTCGCTCC 
TTGGAGCAGG AGAAGGCAGC TTACCAGAAG ACCATTGAAC GCATGCGCTC ATGCCTTCCC 
CTTGACACTC CGACAGATGT GGAGATGACC CAGATCAAAA CAGGTCCCAA CGGGAAAGCC 
AAAACTGAAG CCAAGAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605155.1
CDS	1906..5469
Translation	MESLAEKDNCSSKSGRRFSLTYIGWSALDRRTTLPMLPWLVAEIRRRSEKGDCGPVVQPREVHLVLNPPFVRCVPSSSTNSSVFIFEHKAQLISRFIHNSNDLTYFAYLLRGQPDNPESQMSCHVFKACDPNQVSEVICRIRQVSKSVLKGDAKPKQEADEAFYNSQKFEVLYCGKVTVMHKKAPPTLVDDCIDKFHQHEVEQKRLRLLNGQRGSMEHNPVEFLIGGEGDKVPPSLTERGEDTESAGVDDTAGVQSLCSRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQMKPGEGVIMGPPRRRHSSAPNHVQPSNAEKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGIKHSDHFGFICRDQLDSGPSQYVCFVFQCASESLVEEVMLTLKQAFSTAAALQSNKTEIQLCEACPIHDLHKLCERIEGLYPPRAKIAIQKYLSQLTDNEQAEIFERVQKLKPRSDQEENELVIFHLRQLCDNKQTSHVHIGENAVSSASGADSSASASRFKLDLLKNKARTSLTNSLENIFARGASRMRGRLGSMGSIGSFERQDEESPGPSPPGSPPTFAENDPENGVQFRRRAHTFSHPPVKKRISFEGQSNSQSKAALHRQQSVNPELLQSSNGEGRKRTLSSCSNDSLSWGLPPTPRRVSWRQKIFLRVASPMSKSPAPMHHPDDSKGQELLPLSPHSRRSVMDPSRHLLPPGEKPKRTSADYRALWKTAIHQQILLLRMEKENQRLEEASRDELHIRKMKLNYQEVGQCSKDAQALWERKLTAPGRTTVQQDKKELYHALCQGVPKNRRGEVWLLLSYQHRLRHRLPQRLHAPDTPYQDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFDMLRFLMYDLGIRQQYKPDMISLQIQMYQLSRLLHDYHRELYTHLEDHEISPSLYAAPWFLTLFASQFPLGFVSRIFDFVFVQGTGVIFKVALCLLSSHEGEIMECDSFESIVDYLKSTLPALSQSQMEQTIAKVVDMDISKQLHAYEVEYHVLQDEMLDAGAVLDDSDRLDKLEKTNMQLKRQNMDLLEKLQAARQKIQSLETSVENFLSRESKMKHMIRSLEQEKAAYQKTIERMRSCLPLDTPTDVEMTQIKTGPNGKAKTEAKKP

Target ORF information:

RefSeq Version	XM_011606853.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes TBC1 domain family, member 4 (tbc1d4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606853.1

ORF Insert Sequence:

ATGGAGAGTT TGGCAGAGAA GGACAACTGT TCTTCCAAAT CCGGCAGGAG GTTCTCCTTG 
ACTTACATCG GCTGGTCCGC GCTGGACAGG CGGACCACTC TGCCCATGCT GCCGTGGCTG 
GTGGCTGAGA TCCGCAGGAG GAGCGAGAAG GGCGACTGCG GCCCCGTGGT GCAGCCGAGG 
GAGGTGCACC TGGTCCTTAA TCCCCCTTTT GTCCGCTGCG TCCCCTCCAG CAGCACCAAC 
TCCTCGGTTT TTATTTTTGA ACACAAAGCG CAGCTCATCT CTCGCTTCAT CCACAACAGC 
AATGACCTCA CATATTTTGC TTATCTCCTG CGGGGCCAGC CGGACAACCC GGAGTCCCAG 
ATGTCTTGTC ACGTATTCAA GGCCTGCGAC CCCAACCAGG TCTCTGAGGT GATTTGCAGA 
ATTCGCCAAG TGTCCAAATC TGTCCTAAAG GGAGATGCCA AACCCAAACA GGAAGCTGAC 
GAAGCCTTCT ACAACTCCCA GAAATTTGAA GTGCTTTACT GTGGCAAGGT GACAGTGATG 
CACAAAAAGG CTCCACCAAC CCTCGTCGAC GACTGCATCG ATAAATTTCA TCAGCATGAG 
GTCGAGCAAA AGCGCCTGCG CCTGCTCAAC GGGCAGCGGG GGTCCATGGA GCACAACCCT 
GTGGAGTTTC TGATTGGAGG GGAAGGAGAC AAAGTCCCCC CTTCCCTGAC TGAGCGAGGA 
GAAGATACGG AAAGTGCCGG AGTAGATGAC ACTGCAGGAG TTCAAAGCCT GTGCAGCAGA 
GGATCTTTTC CTGAGTGCAT CCTGGAGGAT TCTGGGTTCG AGGAGCCTCA GGAGTTCCGT 
ACACGTTGCA GCAGCCTGGC TGGCAGTCTT CAGATGAAAC CAGGGGAAGG TGTCATCATG 
GGCCCCCCAC GCCGCCGACA CTCCAGCGCA CCAAATCACG TTCAGCCGTC CAATGCGGAA 
AAGAACCGCA CGATGCTCTT TCAGGTGGGT CGTTTTGAAG TAAACCTCAT AAGTCCTGAC 
AGTAAAACTG TGGTGTTGGA GAAAAACTTC AAGGATATCT CCTCCTGCTG TCAGGGTATC 
AAGCATTCGG ACCACTTCGG TTTCATCTGT CGGGACCAGC TGGACTCTGG GCCCAGTCAA 
TATGTGTGCT TTGTTTTCCA GTGTGCCAGT GAGTCCCTGG TGGAGGAGGT GATGCTTACG 
TTGAAGCAGG CCTTCTCTAC AGCAGCAGCT CTGCAGAGCA ACAAAACAGA GATCCAGCTA 
TGTGAAGCCT GCCCCATTCA TGACCTGCAC AAACTCTGTG AGCGGATTGA AGGTCTCTAC 
CCACCCAGAG CAAAGATAGC CATCCAAAAA TATCTCTCCC AGCTCACCGA TAATGAACAG 
GCTGAGATTT TTGAACGGGT TCAGAAATTA AAGCCCCGGT CAGACCAAGA GGAGAACGAG 
CTGGTGATTT TTCATCTGAG GCAGCTGTGC GACAACAAGC AGACGTCCCA CGTTCACATC 
GGAGAGAATG CAGTGAGCAG CGCGTCAGGA GCAGACTCCT CAGCTAGTGC CAGTCGCTTC 
AAACTTGATC TCCTCAAGAA TAAAGCACGC ACCTCCCTCA CCAACTCTCT GGAGAACATC 
TTTGCAAGGG GTGCCAGCCG GATGCGGGGG CGCTTGGGAA GCATGGGGAG CATTGGCAGT 
TTTGAAAGGC AGGATGAGGA ATCTCCGGGG CCCTCCCCTC CAGGTTCTCC CCCCACCTTT 
GCTGAAAATG ACCCGGAAAA TGGCGTCCAG TTCCGCCGGC GTGCCCACAC CTTCAGCCAC 
CCACCTGTGA AGAAACGCAT CTCCTTCGAG GGCCAGTCAA ACAGCCAGAG CAAGGCTGCC 
CTGCACAGGC AACAGTCTGT AAACCCCGAG CTGCTCCAGA GCAGCAACGG TGAAGGCAGG 
AAGAGAACCC TTTCTAGCTG CAGTAATGAC TCGCTGAGCT GGGGGCTGCC TCCGACCCCA 
CGCAGGGTCT CCTGGAGACA GAAGATCTTC CTCAGGGTTG CATCACCGAT GAGCAAATCA 
CCTGCACCCA TGCACCACCC AGACGACTCT AAAGGCCAGG AGTTGTTGCC TCTTTCTCCA 
CATTCAAGAA GATCTGTTAT GGACCCATCA AGGCATCTGC TTCCACCGGG GGAGAAGCCC 
AAGAGGACCT CAGCTGACTA TCGGGCCCTA TGGAAAACTG CCATCCACCA GCAGATCCTA 
CTTCTGCGCA TGGAAAAGGA GAACCAGAGA CTGGAAGAAG CGAGCCGTGA TGAACTGCAC 
ATACGCAAGA TGAAACTGAA CTATCAGGAA GTGGGCCAGT GCTCCAAAGA TGCTCAGGCA 
CTGTGGGAGA GAAAACTAAC AGCCCCAGGC AGAACAACTG TTCAGCAAGA TAAGAAGGAG 
TTATACCATG CGCTCTGCCA AGGTGTTCCC AAGAACAGAC GTGGGGAAGT TTGGTTGCTT 
CTTTCCTATC AGCATCGGCT CCGCCACAGG CTGCCCCAGC GTCTGCATGC CCCAGACACA 
CCCTATCAGG ATTTGCTGAA GCAGCTGACC GCGCAGCAAC ATGCCATTCT GGTGGATCTA 
GGGCGGACCT TCCCCACCCA CCAATACTTC TCAGCCCAGC TGGGTGCAGG GCAACTTTCC 
CTCTACAACC TCCTTAAAGC TTATTCTCTG CTGGATACAG AGGTTGGTTA CTGTCAGGGT 
ATCAGCTTTG TAGCCGGAGT GCTGCTGCTT CACATGAGCG AGGAGCAGGC CTTTGACATG 
CTGAGGTTCC TCATGTATGA CCTGGGCATC AGGCAGCAGT ACAAACCAGA CATGATCTCT 
CTGCAGATTC AGATGTACCA GCTCTCCAGA CTGCTCCATG ACTACCACCG TGAGTTGTAC 
ACTCATCTCG AGGATCACGA GATCAGCCCA AGCCTTTACG CAGCACCGTG GTTCCTCACA 
CTCTTCGCCT CACAGTTCCC ACTCGGCTTT GTGTCGCGTA TTTTTGATTT TGTATTCGTC 
CAAGGGACTG GAGTGATATT TAAAGTGGCA CTCTGTCTGC TGAGCAGCCA TGAGGGGGAG 
ATAATGGAAT GTGACAGCTT TGAGAGCATT GTGGACTATT TAAAATCTAC ACTTCCTGCC 
CTCAGTCAGT CACAGATGGA ACAGACTATT GCAAAGGTCG TGGATATGGA CATCTCCAAG 
CAACTGCACG CATATGAAGT GGAGTACCAT GTCCTGCAGG ATGAGATGCT GGATGCAGGT 
GCTGTGTTGG ATGACTCTGA CCGTCTTGAC AAGCTGGAAA AGACCAACAT GCAGCTGAAA 
AGGCAAAACA TGGACCTGCT GGAAAAACTT CAGGCTGCGA GGCAGAAGAT TCAGTCGCTG 
GAGACGAGTG TGGAGAACTT CCTTTCTCGG GAGAGCAAAA TGAAGCACAT GATTCGCTCC 
TTGGAGCAGG AGAAGGCAGC TTACCAGAAG ACCATTGAAC GCATGCGCTC ATGCCTTCCC 
CTTGACACTC CGACAGATGT GGAGATGACC CAGATCAAAA CAGGTCCCAA CGGGAAAGCC 
AAAACTGAAG CCAAGAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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オンライン注文

tbc1d4 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム