EGR 1 Antibody, mAb, Rabbit
EGR 1 Antibody, MAb, Rabbit

Western blot analysis of Egr1 expression in 293T cell lysate treated with 20% FBS.

EGR 1 Antibody, mAb, Rabbit

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status.
A03984
¥37,309.00

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Overview
Specificity This product is specific to EGR 1
Host Species Rabbit
Immunogen A synthesized peptide derived from human EGR 1
Species Reactivity Human
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:5000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • EGR 1 Antibody, MAb, Rabbit
    • EGR 1 Antibody, MAb, Rabbit

    Western blot analysis of Egr1 expression in 293T cell lysate treated with 20% FBS.


Background
Target Background Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status.
Synonyms TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR1;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.