E2F1 Antibody, mAb, Rabbit
E2F1 Antibody, MAb, Rabbit

Western blot analysis of E2F1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

E2F1 Antibody, mAb, Rabbit

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
A03393
¥37,309.00

Ask us a question
Overview
Specificity This product is specific to E2F1
Host Species Rabbit
Immunogen A synthesized peptide derived from human E2F1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • E2F1 Antibody, MAb, Rabbit
    • E2F1 Antibody, MAb, Rabbit

    Western blot analysis of E2F1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.


Background
Target Background Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
Synonyms E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.