Cleaved-Caspase 3 p17 Antibody, mAb, Rabbit
Cleaved-Caspase 3 P17 Antibody, MAb, Rabbit

Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin.

Cleaved-Caspase 3 p17 Antibody, mAb, Rabbit

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis.Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins.
A02441
¥37,309.00

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Overview
Specificity This product is specific to Cleaved-Caspase 3 p17
Host Species Rabbit
Immunogen A synthesized peptide derived from human Caspase 3
Species Reactivity Human
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Cleaved-Caspase 3 P17 Antibody, MAb, Rabbit
    • Cleaved-Caspase 3 P17 Antibody, MAb, Rabbit

    Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin.


Background
Target Background Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis.Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins.
Synonyms Apopain precursor; Cysteine protease CPP32; ICE3; CASP-3; CPP32; Caspase-3; SCA-1;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.