Histone H3 Antibody, mAb, Rabbit
Histone H3 Antibody, MAb, Rabbit

Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate.

Histone H3 Antibody, mAb, Rabbit

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
A02399
¥18,121.00

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Overview
Specificity This product is specific to Histone H3
Host Species Rabbit
Immunogen A synthesized peptide derived from human Histone H3.1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Histone H3 Antibody, MAb, Rabbit
    • Histone H3 Antibody, MAb, Rabbit

    Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate.


Background
Target Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms Histone H3.1, Histone H3, HIST1H3A;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.