Vinculin Antibody, mAb, Rabbit
Vinculin Antibody, MAb, Rabbit

All lanes use Vinculin Antibody, mAb, Rabbit at 1:5K dilution for 1 hour at room temperature.

Vinculin Antibody, mAb, Rabbit

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
A02392
¥18,121.00

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Overview
Specificity This product is specific to Vinculin
Host Species Rabbit
Immunogen A synthesized peptide derived from human Vinculin
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:5000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Vinculin Antibody, MAb, Rabbit
    • Vinculin Antibody, MAb, Rabbit

    All lanes use Vinculin Antibody, mAb, Rabbit at 1:5K dilution for 1 hour at room temperature.


Background
Target Background Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Synonyms Vinculin;VCL;Metavinculin; MV;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.