PCNA Antibody, mAb, Rabbit
PCNA Antibody, MAb, Rabbit

Western blot analysis of PCNA expression in (1) Hela cell lysate; (2) HepG2 whole cell lysate; (3) U937 whole cell lysate; (4) Mouse spleen lysate with PCNA Antibody, mAb, Rabbit.

PCNA Antibody, mAb, Rabbit

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
A02380
¥18,121.00

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Overview
Specificity This product is specific to PCNA
Host Species Rabbit
Immunogen A synthesized peptide derived from human PCNA
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:5000-1:10000
Immunohistochemistry (IHC) 1:100-1:500
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:100-1:500
Immunohistochemistry Frozen (IHF) 1:100-1:500
Immunoprecipitation (IP) 1:50-1:100
Flow Cytometry 1:100-1:500

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • PCNA Antibody, MAb, Rabbit
    • PCNA Antibody, MAb, Rabbit

    Western blot analysis of PCNA expression in (1) Hela cell lysate; (2) HepG2 whole cell lysate; (3) U937 whole cell lysate; (4) Mouse spleen lysate with PCNA Antibody, mAb, Rabbit.


Background
Target Background Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
Synonyms Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.