Immobilized HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.9 ng/ml determined by ELISA.

The purity of HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human, His Tag with an affinity constant of 8.49pM as determined in SPR assay (Biacore T200).

HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human

Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.

Cat. No.	Z06575
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
TAT
Price	¥208,928.00


Ask us a question

Product Introduction

Species

Human

Protein Construction

HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer [Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide] Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

258 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

The purity of HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

Background

Target Background	Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
Synonyms	HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His & Avi, Human

サポート

会社情報

サポート

会社情報