Immobilized HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA.

The purity of HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human was greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

Immobilized Tebentafusp, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag with the EC50 of 39.5 ng/ml determined by ELISA.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.

Cat. No.	Z06559
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥208,928.00


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Product Introduction

Species

Human

Protein Construction

HLA-A02:01&B2M&GP100 (YLEPGPVTA) Tetramer [Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide] Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

258 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

The purity of HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human was greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).

Background

Target Background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
Synonyms	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His & Avi, Human

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