HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human
HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&AFP Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA.

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

The purity of HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP TCR with an affinity constant of 0.14 μM as determined in SPR assay (Biacore T200).

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
Z06459
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Product Introduction
Species Human
Protein Construction
HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer [Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide]
Accession # A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 50.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human
    • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

    Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&AFP Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA.

  • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human
    • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

    The purity of HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human
    • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

    HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human
    • HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human

    HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer[Biotin], His & Avi, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP TCR with an affinity constant of 0.14 μM as determined in SPR assay (Biacore T200).


Background
Target Background Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
Synonyms Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.