APRIL/TNFSF13 hFc Chimera, Mouse
APRIL/TNFSF13 HFc Chimera, Mouse

Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind APRIL/TNFSF13 hFc Chimera, Mouse, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay (Gator® Prime).

APRIL/TNFSF13 HFc Chimera, Mouse

Immobilized APRIL/TNFSF13 hFc Chimera, Mouse, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 5.4/5.1ng/ml determined by ELISA.

APRIL/TNFSF13 HFc Chimera, Mouse

The purity of APRIL/TNFSF13 hFc Chimera, Mouse is greater than 95% as determined by SEC-HPLC.

APRIL/TNFSF13 HFc Chimera, Mouse

APRIL/TNFSF13 hFc Chimera, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

APRIL/TNFSF13 hFc Chimera, Mouse

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Z06013
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Product Introduction
Species Mouse
Protein Construction
APRIL/TNFSF13 (Ala96-Leu241)
Accession # Q9D777		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 43.20 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • APRIL/TNFSF13 HFc Chimera, Mouse
    • APRIL/TNFSF13 HFc Chimera, Mouse

    Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind APRIL/TNFSF13 hFc Chimera, Mouse, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay (Gator® Prime).

  • APRIL/TNFSF13 HFc Chimera, Mouse
    • APRIL/TNFSF13 HFc Chimera, Mouse

    Immobilized APRIL/TNFSF13 hFc Chimera, Mouse, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 5.4/5.1ng/ml determined by ELISA.

  • APRIL/TNFSF13 HFc Chimera, Mouse
    • APRIL/TNFSF13 HFc Chimera, Mouse

    The purity of APRIL/TNFSF13 hFc Chimera, Mouse is greater than 95% as determined by SEC-HPLC.

  • APRIL/TNFSF13 HFc Chimera, Mouse
    • APRIL/TNFSF13 HFc Chimera, Mouse

    APRIL/TNFSF13 hFc Chimera, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Synonyms CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.