VEGF121, Human
VEGF121, Human

Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL. (QC Test)

VEGF121, Human

Immobilized VEGF121, Human, No Tag at 0.5 μg/ml (100 μI/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.

VEGF121, Human

The purity of VEGF121, Human is greater than 90% as determined by SEC-HPLC.

VEGF121, Human

VEGF121, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

VEGF121, Human

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
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Product Introduction
Species Human
Protein Construction
VEGF121 (Ala27-Arg147)_x000D_
Accession # P15692-9
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 14.07 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • VEGF121, Human
    • VEGF121, Human

    Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL. (QC Test)

  • VEGF121, Human
    • VEGF121, Human

    Immobilized VEGF121, Human, No Tag at 0.5 μg/ml (100 μI/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.

  • VEGF121, Human
    • VEGF121, Human

    The purity of VEGF121, Human is greater than 90% as determined by SEC-HPLC.

  • VEGF121, Human
    • VEGF121, Human

    VEGF121, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Synonyms VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.