Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag : NKG2A&CD94 mFc Chimera, Human, mFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 0.15μg/ml.

Immobilized NKG2A&CD94 mFc Chimera, Human, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.

The purity of NKG2A&CD94 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

NKG2A&CD94 mFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2A&CD94 mFc Chimera, Human, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).

NKG2A&CD94 mFc Chimera, Human

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Cat. No.	Z05685
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥76,483.00


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Product Introduction

Species

Human

Protein Construction

NKG2A&CD94 (Ala113-Leu233 (NKG2A) and Ser34-Ile179 (CD94)) Accession # P26715-1(NKG2A)&Q13241-1(CD94)	mFc (IgG1)
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

56.9 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized NKG2A&CD94 mFc Chimera, Human, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.

The purity of NKG2A&CD94 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

NKG2A&CD94 mFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

NKG2A&CD94 mFc Chimera, Human

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