Serial dilutions of Anti-NKG2A Antibody were added into NKG2A&CD94, His & Avi, Flag Human, His Tag : Biotinylated Human HLA-E Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 31.8ng/ml.

Immobilized NKG2A&CD94, His & Avi, Flag Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.

The purity of NKG2A&CD94, His & Avi, Flag Human is greater than 95% as determined by SEC-HPLC.

NKG2A&CD94, His & Avi, Flag Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2A&CD94, His & Avi, Flag Human

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Cat. No.	Z05684
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥72,752.00


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Product Introduction

Species

Human

Protein Construction

Avi	His	NKG2A&CD94 (Arg100-Leu233 (NKG2A) and Ser34-Ile179 (CD94))_x000D_ Accession # P26715-1(NKG2A)&Q13241-1(CD94)
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

17.9 kDa (CD94) and 18.3 kDa (NKG2A)

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized NKG2A&CD94, His & Avi, Flag Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.

The purity of NKG2A&CD94, His & Avi, Flag Human is greater than 95% as determined by SEC-HPLC.

NKG2A&CD94, His & Avi, Flag Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

NKG2A&CD94, His & Avi, Flag Human

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