LY75/CD205, His, Human
LY75/CD205, His, Human

Immobilized LY75/CD205, His, Human, His Tag at 0.1 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

LY75/CD205, His, Human

The purity of LY75/CD205, His, Human is greater than 95% as determined by SEC-HPLC.

LY75/CD205, His, Human

LY75/CD205, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

LY75/CD205, His, Human

Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
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Product Introduction
Species Human
Protein Construction
LY75/CD205 (Ser28-Asp1666)
Accession # O60449-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 190.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 230-245 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • LY75/CD205, His, Human
    • LY75/CD205, His, Human

    Immobilized LY75/CD205, His, Human, His Tag at 0.1 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

  • LY75/CD205, His, Human
    • LY75/CD205, His, Human

    The purity of LY75/CD205, His, Human is greater than 95% as determined by SEC-HPLC.

  • LY75/CD205, His, Human
    • LY75/CD205, His, Human

    LY75/CD205, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
Synonyms Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.