IL-6, Human
IL-6, Human

Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind IL-6, Human, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator® Prime).

IL-6, Human

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL. (QC Test)

IL-6, Human

Immobilized IL-6, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.

IL-6, Human

The purity of IL-6, Human is greater than 90% as determined by SEC-HPLC.

IL-6, Human

IL-6, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-6, Human

Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind IL-6, Human, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

IL-6, Human

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.
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Product Introduction
Species Human
Protein Construction
IL-6 (Val30-Met212)
Accession # P05231
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 0.05EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 20.8 kDa
Apparent Molecular Weight The protein has a predicted 20.8 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-6, Human
    • IL-6, Human

    Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind IL-6, Human, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator® Prime).

  • IL-6, Human
    • IL-6, Human

    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL. (QC Test)

  • IL-6, Human
    • IL-6, Human

    Immobilized IL-6, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.

  • IL-6, Human
    • IL-6, Human

    The purity of IL-6, Human is greater than 90% as determined by SEC-HPLC.

  • IL-6, Human
    • IL-6, Human

    IL-6, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-6, Human
    • IL-6, Human

    Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind IL-6, Human, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).


Background
Target Background IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.
Synonyms IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.