IL-21, Human
IL-21, Human

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is <8 ng/mL (QC Test).

IL-21, Human

Immobilized IL-21, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 45.1ng/ml determined by ELISA.

IL-21, Human

The purity of IL-21, Human is greater than 95% as determined by SEC-HPLC.

IL-21, Human

IL-21, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-21, Human

Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Z05475
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Product Introduction
Species Human
Protein Construction
IL-21 (Gln32-Ser162)
Accession # Q9HBE4-1
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 15.4 kDa
Apparent Molecular Weight The protein has a predicted MW of 15.4 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5).
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-21, Human
    • IL-21, Human

    Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is <8 ng/mL (QC Test).

  • IL-21, Human
    • IL-21, Human

    Immobilized IL-21, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 45.1ng/ml determined by ELISA.

  • IL-21, Human
    • IL-21, Human

    The purity of IL-21, Human is greater than 95% as determined by SEC-HPLC.

  • IL-21, Human
    • IL-21, Human

    IL-21, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Synonyms Interleukin-21; IL-21; Za11; CVID11

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.