Immobilized Human IL-15Ra&IL-15, His Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for IL-2 R beta&IL-2 R gamma hFc Chimera, Human, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA.
IL-2 R beta&IL-2 R gamma hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
IL-2 R beta&IL-2 R gamma hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
IL-2 R beta&IL-2 R gamma hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
IL-2 R beta&IL-2 R gamma hFc Chimera, Human
Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change
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