IL-18, Human
IL-18, Human

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL (QC Test).

IL-18, Human

Immobilized IL-18, Human, No Tag at 5 μg/ml (100μl/well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.2ng/ml determined by ELISA.

IL-18, Human

The purity of IL-18, Human is greater than 95% as determined by SEC-HPLC.

IL-18, Human

IL-18, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-18, Human

IL-18, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).

IL-18, Human

Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Z05450
¥69,021.00

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Product Introduction
Species Human
Protein Construction
IL-18 (Tyr37-Asp193)
Accession # Q14116
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 0.1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 18.2 kDa
Apparent Molecular Weight The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-18, Human
    • IL-18, Human

    Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL (QC Test).

  • IL-18, Human
    • IL-18, Human

    Immobilized IL-18, Human, No Tag at 5 μg/ml (100μl/well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.2ng/ml determined by ELISA.

  • IL-18, Human
    • IL-18, Human

    The purity of IL-18, Human is greater than 95% as determined by SEC-HPLC.

  • IL-18, Human
    • IL-18, Human

    IL-18, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-18, Human
    • IL-18, Human

    IL-18, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).


Background
Target Background Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Synonyms IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.