IL-12, His & Avi, Human
IL-12, His & Avi, Human

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1 - 1 ng/mL.

IL-12, His & Avi, Human

Immobilized IL-12, His & Avi, Human, His Tag at 1 μg/ml (100 μI/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.

IL-12, His & Avi, Human

The purity of IL-12, His & Avi, Human is greater than 90% as determined by SEC-HPLC.

IL-12, His & Avi, Human

IL-12, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-12, His & Avi, Human

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Z05429
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Product Introduction
Species Human
Protein Construction
IL-12 [Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A)]
Accession # P29460 (IL-12B) & P29459 (IL-12A)		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-12, His & Avi, Human
    • IL-12, His & Avi, Human

    Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1 - 1 ng/mL.

  • IL-12, His & Avi, Human
    • IL-12, His & Avi, Human

    Immobilized IL-12, His & Avi, Human, His Tag at 1 μg/ml (100 μI/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.

  • IL-12, His & Avi, Human
    • IL-12, His & Avi, Human

    The purity of IL-12, His & Avi, Human is greater than 90% as determined by SEC-HPLC.

  • IL-12, His & Avi, Human
    • IL-12, His & Avi, Human

    IL-12, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Synonyms CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.