IL-11, His, Human
IL-11, His, Human

Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 1-8 ng/mL. (QC Test)

IL-11, His, Human

Immobilized IL-11, His, Human, His Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

IL-11, His, Human

The purity of IL-11, His, Human is greater than 95% as determined by SEC-HPLC.

IL-11, His, Human

IL-11, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-11, His, Human

Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind IL-11, His, Human, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).

IL-11, His, Human

Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers, human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.
Z05427
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Product Introduction
Species Human
Protein Construction
His IL-11 (Pro22-Leu199)
Accession # P20809-1
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 20.24 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid (pH 3.0).
Reconstitution Dissolve the lyophilized protein in 20 mM Citric acid (pH 3.0).It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/ml for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-11, His, Human
    • IL-11, His, Human

    Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 1-8 ng/mL. (QC Test)

  • IL-11, His, Human
    • IL-11, His, Human

    Immobilized IL-11, His, Human, His Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

  • IL-11, His, Human
    • IL-11, His, Human

    The purity of IL-11, His, Human is greater than 95% as determined by SEC-HPLC.

  • IL-11, His, Human
    • IL-11, His, Human

    IL-11, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-11, His, Human
    • IL-11, His, Human

    Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind IL-11, His, Human, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).


Background
Target Background Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers, human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.
Synonyms Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.