IL-10, Human
IL-10, Human

Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test).

IL-10, Human

Immobilized IL-10, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 59.2ng/ml determined by ELISA.

IL-10, Human

The purity of IL-10, Human is greater than 95% as determined by SEC-HPLC.

IL-10, Human

IL-10, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-10, Human

Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind IL-10, Human, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).

IL-10, Human

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
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Product Introduction
Species Human
Protein Construction
IL-10 (Ser19-Asn178)
Accession # P22301
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 0.1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 18.8 kDa
Apparent Molecular Weight The protein has a predicted MW of 18.8 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-10, Human
    • IL-10, Human

    Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test).

  • IL-10, Human
    • IL-10, Human

    Immobilized IL-10, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 59.2ng/ml determined by ELISA.

  • IL-10, Human
    • IL-10, Human

    The purity of IL-10, Human is greater than 95% as determined by SEC-HPLC.

  • IL-10, Human
    • IL-10, Human

    IL-10, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-10, Human
    • IL-10, Human

    Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind IL-10, Human, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).


Background
Target Background Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
Synonyms Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.