IFN alpha 1 hFc Chimera, Human
IFN Alpha 1 HFc Chimera, Human

The purity of IFN alpha 1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

IFN Alpha 1 HFc Chimera, Human

IFN alpha 1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IFN Alpha 1 HFc Chimera, Human

Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind IFN alpha 1 hFc Chimera, Human, hFc Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200) (QC Test).

IFN Alpha 1 HFc Chimera, Human

Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind IFN alpha 1 hFc Chimera, Human, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test).

IFN alpha 1 hFc Chimera, Human

IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
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Product Introduction
Species Human
Protein Construction
IFN alpha 1 (Cys24-Glu189)
Accession # P01562		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti­his antibody can bind IFN alpha 1 hFc Chimera, Human in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 46.14 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IFN Alpha 1 HFc Chimera, Human
    • IFN Alpha 1 HFc Chimera, Human

    The purity of IFN alpha 1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • IFN Alpha 1 HFc Chimera, Human
    • IFN Alpha 1 HFc Chimera, Human

    IFN alpha 1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IFN Alpha 1 HFc Chimera, Human
    • IFN Alpha 1 HFc Chimera, Human

    Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind IFN alpha 1 hFc Chimera, Human, hFc Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200) (QC Test).

  • IFN Alpha 1 HFc Chimera, Human
    • IFN Alpha 1 HFc Chimera, Human

    Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind IFN alpha 1 hFc Chimera, Human, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test).


Background
Target Background IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
Synonyms Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.