B7-1/CD80, His & Avi, Human
B7-1/CD80, His & Avi, Human

Immobilized Human CTLA-4, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for B7-1/CD80, His & Avi, Human, His Tag with the EC50 of 1.33μg/ml determined by ELISA.

B7-1/CD80, His & Avi, Human

The purity of  B7-1/CD80, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-1/CD80, His & Avi, Human

B7-1/CD80, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-1/CD80, His & Avi, Human

B7-1/CD80, His & Avi, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).

B7-1/CD80, His & Avi, Human

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Z05053
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Product Introduction
Species Human
Protein Construction
B7-1/CD80 (Val35-Asn242)
Accession # P33681-1		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • B7-1/CD80, His & Avi, Human
    • B7-1/CD80, His & Avi, Human

    Immobilized Human CTLA-4, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for B7-1/CD80, His & Avi, Human, His Tag with the EC50 of 1.33μg/ml determined by ELISA.

  • B7-1/CD80, His & Avi, Human
    • B7-1/CD80, His & Avi, Human

    The purity of  B7-1/CD80, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • B7-1/CD80, His & Avi, Human
    • B7-1/CD80, His & Avi, Human

    B7-1/CD80, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • B7-1/CD80, His & Avi, Human
    • B7-1/CD80, His & Avi, Human

    B7-1/CD80, His & Avi, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).


Background
Target Background Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.