Serial dilutions of Activin RIIB/ACVR2B, His, Human/Cynomolgus Antibody were added into Human Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 0.14 μg /ml.

Immobilized Human Activin A, No Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for Activin RIIB/ACVR2B, His, Human/Cynomolgus, His Tag with the EC50 of 11.6ng/ml determined by ELISA.

Immobilized Activin RIIB/ACVR2B, His, Human/Cynomolgus, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat GDF-8, Avi Tag with the EC50 of 2.5ng/ml determined by ELISA.

Immobilized Activin RIIB/ACVR2B, His, Human/Cynomolgus, His Tag at 0.5 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

The purity of Activin RIIB/ACVR2B, His, Human/Cynomolgus is greater than 95% as determined by SEC-HPLC.

Activin RIIB/ACVR2B, His, Human/Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Activin RIIB/ACVR2B, His, Human/Cynomolgus, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A with an affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).

Activin RIIB/ACVR2B, His, Human/Cynomolgus

ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).

Cat. No.	Z04990
Size	100 μg : Quote 500 μg : Quote Buy in bulk
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Price	¥35,443.00


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Product Introduction

Species

Human/Cynomolgus

Protein Construction

Activin RIIB/ACVR2B [Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus)] Accession # Q13705-1 (Human) / XP_045242398.1 (Cynomolgus)	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

14.75 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 25-45 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

The purity of Activin RIIB/ACVR2B, His, Human/Cynomolgus is greater than 95% as determined by SEC-HPLC.

Activin RIIB/ACVR2B, His, Human/Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background	ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
Synonyms	Activin receptor type-2B; ACVR2B; Activin RIIB

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Activin RIIB/ACVR2B, His, Human/Cynomolgus

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