IL-4, His, Cynomolgus
IL-4, His, Cynomolgus

Immobilized IL-4, His, Cynomolgus, His Tag at 2 μg /ml (100 μl /well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA.

IL-4, His, Cynomolgus

The purity of IL-4, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

IL-4, His, Cynomolgus

IL-4, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-4, His, Cynomolgus

IL-4, His, Cynomolgus, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).

IL-4, His, Cynomolgus

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.
Z04843
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Product Introduction
Species Cynomolgus
Protein Construction
IL-4 (His25-Ser153)
Accession # P79339-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 16.03 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-4, His, Cynomolgus
    • IL-4, His, Cynomolgus

    Immobilized IL-4, His, Cynomolgus, His Tag at 2 μg /ml (100 μl /well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA.

  • IL-4, His, Cynomolgus
    • IL-4, His, Cynomolgus

    The purity of IL-4, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

  • IL-4, His, Cynomolgus
    • IL-4, His, Cynomolgus

    IL-4, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-4, His, Cynomolgus
    • IL-4, His, Cynomolgus

    IL-4, His, Cynomolgus, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).


Background
Target Background Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.
Synonyms IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.