Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus
Alkaline Phosphatase (Placental Type) /ALPP, His, Cynomolgus

Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Alkaline Phosphatase (Placental Type) /ALPP, His, Cynomolgus

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus, His Tag with an affinity constant of 0.11 μM as determined in SPR assay (Biacore T200).

Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus

Alkaline phosphatase can be considered 'our favorite enzyme' for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Z04729
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Product Introduction
Species Cynomolgus
Protein Construction
Alkaline Phosphatase (Placental type) /ALPP (Ile21-Thr503)
Accession # XP_045223825.1		 His
N-term C-term
Purity > 95% as determined by Bis­-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Loaded Anti­-Alkaline Phosphatase Antibody, hFc Tag on ProA-­Biosensor can bind Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus in BLI assay (Gator® Prime). Test result was comparable to standard batch. Measured by its ability to cleave a fluorogenic substrate, 4­Methylumbelliferyl phosphate (4­ MUP). Test result met the standard.
Expression System HEK293
Theoretical Molecular Weight 53.69 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • Alkaline Phosphatase (Placental Type) /ALPP, His, Cynomolgus
    • Alkaline Phosphatase (Placental Type) /ALPP, His, Cynomolgus

    Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • Alkaline Phosphatase (Placental Type) /ALPP, His, Cynomolgus
    • Alkaline Phosphatase (Placental Type) /ALPP, His, Cynomolgus

    Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus, His Tag with an affinity constant of 0.11 μM as determined in SPR assay (Biacore T200).


Background
Target Background Alkaline phosphatase can be considered 'our favorite enzyme' for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Synonyms Alkaline phosphatase; PLAP-1; ALPP; PLAP

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.