Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bindIL-22[Biotin], His & Avi, Mouse, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).

Immobilized IL-22[Biotin], His & Avi, Mouse, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.

The purity of IL-22[Biotin], His & Avi, Mouse is greater than 90% as determined by SEC-HPLC.

IL-22[Biotin], His & Avi, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-22[Biotin], His & Avi, Mouse, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 μM as determined in SPR assay (Biacore T200).

IL-22[Biotin], His & Avi, Mouse

The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.

Cat. No.	Z04670
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
TAT
Price	¥123,118.00


Ask us a question

Product Introduction

Species

Mouse

Protein Construction

Avi	His	IL-22 (Leu34-Val179) Accession # Q9JJY9
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

E.coli

Theoretical Molecular Weight

19.7 kDa

Apparent Molecular Weight

The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bindIL-22[Biotin], His & Avi, Mouse, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).

The purity of IL-22[Biotin], His & Avi, Mouse is greater than 90% as determined by SEC-HPLC.

IL-22[Biotin], His & Avi, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-22[Biotin], His & Avi, Mouse, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 μM as determined in SPR assay (Biacore T200).

Background

Target Background	The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Synonyms	Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

IL-22[Biotin], His & Avi, Mouse

サポート

会社情報

サポート

会社情報