Siglec-4a/MAG[Biotin], His & Avi, Human
Siglec-4a/MAG[Biotin], His & Avi, Human

Immobilized Siglec-4a/MAG[Biotin], His & Avi, Human, hFc Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA.

Siglec-4a/MAG[Biotin], His & Avi, Human

The purity of Siglec-4a/MAG[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Siglec-4a/MAG[Biotin], His & Avi, Human

Siglec-4a/MAG[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Siglec-4a/MAG[Biotin], His & Avi, Human

Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
Z04598
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Product Introduction
Species Human
Protein Construction
Siglec-4a/MAG (Gly20-Pro516)
Accession # P20916		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 57.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • Siglec-4a/MAG[Biotin], His & Avi, Human
    • Siglec-4a/MAG[Biotin], His & Avi, Human

    Immobilized Siglec-4a/MAG[Biotin], His & Avi, Human, hFc Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA.

  • Siglec-4a/MAG[Biotin], His & Avi, Human
    • Siglec-4a/MAG[Biotin], His & Avi, Human

    The purity of Siglec-4a/MAG[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • Siglec-4a/MAG[Biotin], His & Avi, Human
    • Siglec-4a/MAG[Biotin], His & Avi, Human

    Siglec-4a/MAG[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
Synonyms MAG; Siglec4a; Siglec-4a; S-MAG; GMA

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.