Loaded Anti-SEZ6 Antibody, hFc Tag on ProA-Biosensor can bind SEZ6[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 3.31 nM as determined in BLI assay (Gator® Prime).

Immobilized SEZ6[Biotin], His & Avi, Human, His-Avi Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA.

The purity of SEZ6[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

SEZ6[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEZ6[Biotin], His & Avi, Human

Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.

Cat. No.	Z04594
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥123,118.00


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Product Introduction

Species

Human

Protein Construction

SEZ6 (Leu20-His925) Accession # Q53EL9-1	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

100.7 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 170-200 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Loaded Anti-SEZ6 Antibody, hFc Tag on ProA-Biosensor can bind SEZ6[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 3.31 nM as determined in BLI assay (Gator® Prime).

The purity of SEZ6[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

SEZ6[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background	Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
Synonyms	SEZ-6; SEZ6; BSRPC; BSRP-C

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

SEZ6[Biotin], His & Avi, Human

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