IL-3[Biotin], His & Avi, Human
IL-3[Biotin], His & Avi, Human

Immobilized IL-3[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0 ng/ml determined by ELISA.

IL-3[Biotin], His & Avi, Human

The purity of IL-3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

IL-3[Biotin], His & Avi, Human

IL-3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-3[Biotin], His & Avi, Human

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Z04512
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Product Introduction
Species Human
Protein Construction
Avi His IL-3 (Ala20-Phe152)
Accession # P08700
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 16.9 kDa
Apparent Molecular Weight The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-3[Biotin], His & Avi, Human
    • IL-3[Biotin], His & Avi, Human

    Immobilized IL-3[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0 ng/ml determined by ELISA.

  • IL-3[Biotin], His & Avi, Human
    • IL-3[Biotin], His & Avi, Human

    The purity of IL-3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • IL-3[Biotin], His & Avi, Human
    • IL-3[Biotin], His & Avi, Human

    IL-3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Synonyms IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.