ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human
ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human, His Tag with the EC50 of 10.2 ng/ml determined by ELISA.

ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

The purity of ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
Z04398
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Product Introduction
Species Human
Protein Construction
ANGPTL3/Angiopoietin-like 3 (Ser17-Lys219)
Accession # Q9Y5C1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human
    • ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

    Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human, His Tag with the EC50 of 10.2 ng/ml determined by ELISA.

  • ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human
    • ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

    The purity of ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human
    • ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human

    ANGPTL3/Angiopoietin-like 3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.


Background
Target Background ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
Synonyms ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.