ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human
ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

Immobilized Biotinylated Human ANGPT2, His Tag at 1 μg/mL (100 μL/well) on the on the plate. A Dose response curve in ELISA yielded an EC50 of 14.26 ng/mL for Anti-ANGPT2 Antibody, rFc Tag.

ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

The purity of Biotinylated Human ANGPT2 is greater than 90% as determined by SEC-HPLC.

ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human on SDS-PAGE under reducing condition. The purity is greater than 95%.

ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
Z04396
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Product Introduction
Species Human
Protein Construction
Avi His ANGPT2/Angiopoietin-2(Lys275-Phe496)
Accession # O15123-1
N-term C-term
Conjugate Biotin
Purity > 95% as determined by SDS-PAGE
> 90% as determined by HPLC
Endotoxin Level < 1 EU/μg by gel clotting method
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System CHO
Theoretical Molecular Weight 28.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-37 kDa based on SDS-PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human
    • ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

    Immobilized Biotinylated Human ANGPT2, His Tag at 1 μg/mL (100 μL/well) on the on the plate. A Dose response curve in ELISA yielded an EC50 of 14.26 ng/mL for Anti-ANGPT2 Antibody, rFc Tag.

  • ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human
    • ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

    The purity of Biotinylated Human ANGPT2 is greater than 90% as determined by SEC-HPLC.

  • ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human
    • ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human

    ANGPT2/Angiopoietin-2[Biotin], His & Avi, Human on SDS-PAGE under reducing condition. The purity is greater than 95%.


Background
Target Background Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
Synonyms AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.