Siglec-3/CD33 hFc Chimera, Human
Siglec-3/CD33 HFc Chimera, Human

Immobilized Siglec-3/CD33 hFc Chimera, Human, hFc Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.6 ng/ml determined by ELISA.

Siglec-3/CD33 HFc Chimera, Human

The purity of Siglec-3/CD33 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

Siglec-3/CD33 HFc Chimera, Human

Siglec-3/CD33 hFc Chimera, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Siglec-3/CD33 hFc Chimera, Human

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Z04281
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Product Introduction
Species Human
Protein Construction
Siglec-3/CD33 (Asp18-His259)
Accession # P20138-1		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 53.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • Siglec-3/CD33 HFc Chimera, Human
    • Siglec-3/CD33 HFc Chimera, Human

    Immobilized Siglec-3/CD33 hFc Chimera, Human, hFc Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.6 ng/ml determined by ELISA.

  • Siglec-3/CD33 HFc Chimera, Human
    • Siglec-3/CD33 HFc Chimera, Human

    The purity of Siglec-3/CD33 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • Siglec-3/CD33 HFc Chimera, Human
    • Siglec-3/CD33 HFc Chimera, Human

    Siglec-3/CD33 hFc Chimera, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.


Background
Target Background Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Synonyms CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.