CD8 alpha/CD8A[Biotin], His & Avi, Human
CD8 Alpha/CD8A[Biotin], His & Avi, Human

Immobilized CD8 alpha/CD8A[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μL/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.

CD8 Alpha/CD8A[Biotin], His & Avi, Human

CD8 alpha/CD8A[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD8 alpha/CD8A[Biotin], His & Avi, Human

CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Z04073
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Product Introduction
Species Human
Protein Construction
CD8 alpha/CD8A (Ser22-Asp182）
Accession # P01732-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 20.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • CD8 Alpha/CD8A[Biotin], His & Avi, Human
    • CD8 Alpha/CD8A[Biotin], His & Avi, Human

    Immobilized CD8 alpha/CD8A[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μL/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.

  • CD8 Alpha/CD8A[Biotin], His & Avi, Human
    • CD8 Alpha/CD8A[Biotin], His & Avi, Human

    CD8 alpha/CD8A[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Synonyms CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.