Activin A, Human
Activin A, Human

The TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell (Cat.No.M00903) was stimulated with serial dilutions of human Activin A protein. The EC50 was 0.2701 nM.

Activin A, Human

Immobilized Activin A, Human, at 1.4 μg/mL (100μL/well) on the plate. A Dose response curve in ELISA yielded an EC50 of 191.9 ng/mL for Human Activin RIIB, hFc Tag.

Activin A, Human

Immobilized Activin A, Human on SDS- PAGE under reducing (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Activin A, Human

Activin-A, a member of the TGF-β superfamily, has fundamental roles in embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation, and tissue fibrosis. It signals through two type I and two type II receptors, activating kinase activity, phosphorylating SMAD2 and 3 intracellular signaling mediators, forming a complex with SMAD4, and translocating to the nucleus to regulate gene expression.
Z03814
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Product Introduction
Species Human
Protein Construction
Activin A (Gly311-Ser426)
Accession # P08476
Purity > 95% as determined by SDS-PAGE
Endotoxin Level < 0.1 EU/μg by gel clotting method
Biological Activity Binding Activity: Confirmed by functional ELISA (results comparable to standard).
Bioactivity: Determined by dose-dependent stimulation in TGF-β/SMAD SBE Reporter-HEK293 cells (Cat. No. M00903), analyzed via a 4PL curve fit.
Expression System CHO
Theoretical Molecular Weight 12.97 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 13-17 kDa under reduced (R) condition, and 26-34 kDa under Non reducing (N) condition based on SDS- PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 4 mM HCI.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI.
Storage & Stability Upon receiving, the lyophilized product remains stable for 6 months at -20℃ or below as supplied from the date of receipt. -80℃ for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Examples
  • Activin A, Human
    • Activin A, Human

    The TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell (Cat.No.M00903) was stimulated with serial dilutions of human Activin A protein. The EC50 was 0.2701 nM.

  • Activin A, Human
    • Activin A, Human

    Immobilized Activin A, Human, at 1.4 μg/mL (100μL/well) on the plate. A Dose response curve in ELISA yielded an EC50 of 191.9 ng/mL for Human Activin RIIB, hFc Tag.

  • Activin A, Human
    • Activin A, Human

    Immobilized Activin A, Human on SDS- PAGE under reducing (R) condition and Non reducing (N) condition. The purity is greater than 95%.


Background
Target Background Activin-A, a member of the TGF-β superfamily, has fundamental roles in embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation, and tissue fibrosis. It signals through two type I and two type II receptors, activating kinase activity, phosphorylating SMAD2 and 3 intracellular signaling mediators, forming a complex with SMAD4, and translocating to the nucleus to regulate gene expression.
Synonyms INHBA; Activin A;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.