GenCRISPR™ ゲノム編集関連製品 » GenCRISPR™ ヌクレアーゼ-CRISPR遺伝子編集 » GenCrispr Cas9 Nuclease
GenCrispr Cas9 Nuclease

In vitro DNA cleavage assay with Cas9 nuclease
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is EcoRV-linearized pUC57 plasmid DNA

GenCrispr Cas9 Nuclease

GenCrispr Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With GenCrispr Cas9 nuclease, customers can screen highly efficient gRNA using in vitro DNA cleavage assays. It could be a powerful tool for gene editing. Product Source : GenCrispr Cas9 Nuclease is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes without nuclear localization signal (NLS). With this Cas9 Nuclease, you can: Screening for highly efficient and specific targeting gRNAs by in vitro DNA cleavage using Cas9 Nuclease from S. pyogenes ( please find the detailed protocol in the product manual ). Highly purified Cas9 antigen could be used for specific antibody production.
Z03386
¥6,344.00

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Description

GenCrispr Cas9 Nuclease is the recombinant Streptococcus pyogenes_x000D_ Cas9 (wt) protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With GenCrispr Cas9 nuclease, customers can screen highly efficient gRNA using in vitro_x000D_ DNA cleavage assays. It could be a powerful tool for gene editing._x000D_
_x000D_ _x000D_
Product Source :_x000D_ GenCrispr Cas9 Nuclease is produced by expression in an_x000D_ E. coli_x000D_ strain carrying a plasmid encoding the Cas9 gene from_x000D_ Streptococcus pyogenes_x000D_ without nuclear localization signal (NLS)._x000D_

_x000D_ _x000D_ With this Cas9 Nuclease, you can:_x000D_

    _x000D_ _x000D_
  1. Screening for highly efficient and specific targeting gRNAs by_x000D_ in vitro_x000D_ DNA cleavage using Cas9 Nuclease from_x000D_ S. pyogenes_x000D_ (_x000D_ please find the detailed protocol in the product manual _x000D_ )._x000D_
  2. _x000D_ _x000D_ Highly purified Cas9 antigen could be used for specific antibody production._x000D_

Note

1000 nM is equal to 160 ng/ul.

Properties
Storage & Stability GenCrispr Cas9 is supplied with 1x storage buffer (10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH7.4 at 25℃) and is recommended to be stored at -20℃.

Examples
  • GenCrispr Cas9 Nuclease
    • GenCrispr Cas9 Nuclease

    In vitro DNA cleavage assay with Cas9 nuclease
    Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is EcoRV-linearized pUC57 plasmid DNA