MonoRab™ Anti-Camelid VHH Affinity Resin
MonoRab™ Anti-Camelid VHH Affinity Resin

Figure 1. Purification of Camelid VHH#1 from E. coli lysate

Lanes:
1. Protein marker
2. Whole cell lysate
3. Flow through
4. Washing eluate(first time)
5. Washing eluate(final-time)
6. Eluate of target Camelid VHH #1 (M.W.=15 kDa monomer and 30 kDa dimer)

MonoRab™ Anti-Camelid VHH Affinity Resin

Figure 2. Purification of Camelid VHH#2 from E. coli lysate

Lanes:
1. Protein marker
2. Flow through 1
3. Flow through 2
4. Eluate 1 of target Camelid VHH #2 (M.W.=15 kDa)
5. Eluate 2 of target Camelid VHH #2 (M.W.=15 kDa)
6. Empty elution buffer
7. Whole cell lysate

MonoRab™ Anti-Camelid VHH Affinity Resin

GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells.
L00905
¥66,975.00

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Overview
Product description Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This singledomain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity.
GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified Camelid VHH single domain antibody is eluted with a high recovery rate. Table 1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin.
Key Features ● Small size, simple production and high affinity
● Can be recycled at least 5 times with no loss of binding capability
● Can be reused up to 10 times with minimum loss of binding capability
● Acid buffer elution (pH 2.5)

Properties
Formulation 50% settled resin in TBS with 0.02% sodium azide
Matrix 4% cross-linked agarose
Average Particle Size 90 μm (45-165 μm)
Ligand MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb
Binding Capacity Approximately 2-4 mg Camelid VHH protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs)
Elution Method Acid buffer elution (pH 2.5)
Reuse Capability When stored at 2 - 8° C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability.
Reagents Compatibility Compatible with commonly used bacterial lysis reagents.
Application purification of Camelid VHH single domain antibody
Storage Stored at 2°C~8°C. Do not freeze.

Examples
  • MonoRab™ Anti-Camelid VHH Affinity Resin
    • MonoRab™ Anti-Camelid VHH Affinity Resin

    Figure 1. Purification of Camelid VHH#1 from E. coli lysate

    Lanes:
    1. Protein marker
    2. Whole cell lysate
    3. Flow through
    4. Washing eluate(first time)
    5. Washing eluate(final-time)
    6. Eluate of target Camelid VHH #1 (M.W.=15 kDa monomer and 30 kDa dimer)

  • MonoRab™ Anti-Camelid VHH Affinity Resin
    • MonoRab™ Anti-Camelid VHH Affinity Resin

    Figure 2. Purification of Camelid VHH#2 from E. coli lysate

    Lanes:
    1. Protein marker
    2. Flow through 1
    3. Flow through 2
    4. Eluate 1 of target Camelid VHH #2 (M.W.=15 kDa)
    5. Eluate 2 of target Camelid VHH #2 (M.W.=15 kDa)
    6. Empty elution buffer
    7. Whole cell lysate


Terms of Use
Terms of Use This product may be the subject of one or more patents filed by GenScript Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer should not sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. For commercial use, please contact GenScript at [email protected].

For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.