Protein Isolation and Analysis » タンパク質単離・精製 » 精製用レジン » Protein L Resin

Protein L Resin

GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
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Description

GenScript Protein L Resin is an affinity chromatography medium designed_x000D_ for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.  The highly purified protein L ligand is_x000D_ coupled to 4% agarose. The coupling is_x000D_ optimized to give high binding capacity for immunoglobulins._x000D_ The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending_x000D_ on several factors such as target antibody, flow rate etc. 


_x000D_ _x000D_ Protein L, first isolated from the surface of_x000D_ bacterial species Peptostreptoccus magnus,_x000D_ binds immunoglobulin through κ light chain interaction. Protein L binds a wider_x000D_ range of Ig classes and subclasses than other antibody-binding proteins such as_x000D_ protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA,_x000D_ IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab_x000D_ fragments. 

Note

High temperature heating is not recommended. The agarose melts above 65°C.

Overview
Key Features Characteristics of Protein L Resin
Matrix spherical4% agarose
Ligandhighly purified protein L
Binding capacity
≥ 15 mg Human IgG/ml settled resin
Average particle size90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol 

Properties
Storage & Stability Store regenerated Protein L Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.