LOC108712509 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108712509
Entrez Gene ID	108712509
Full Name	pleckstrin homology domain-containing family A member 5-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018254709.1	XP_018110198.1	pleckstrin homology domain-containing family A member 5-like isoform X1
XM_018254710.1	XP_018110199.1	pleckstrin homology domain-containing family A member 5-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC108712509 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108712509 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf26231	XM_018254709.1 Latest version!	Xenopus laevis pleckstrin homology domain-containing family A member 5-like (LOC108712509), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAf26232	XM_018254710.1 Latest version!	Xenopus laevis pleckstrin homology domain-containing family A member 5-like (LOC108712509), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108712509 ( XM_018254709.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018110198.1 Xenopus laevis pleckstrin homology domain-containing family A member 5-like (LOC108712509), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf26231
Clone ID Related Accession (Same CDS sequence)	XM_018254709.1
Accession Version	XM_018254709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	pleckstrin homology domain-containing family A member 5-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030729.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGTGTATTTG TGTAACACAA GTCCTTGTCT CCCCCAGTGA GGAAGCAAAG 
AGTACCACCT GGCTGCACCC GCTCACTGGG GAGACTCTAC TCACAGGCCA CAGAAAGACC 
CCAGACTTGC CCACAGGGTG GCAGGAGGGC TACACGTTTG AAGGCGCTCG ATACTACATA 
AACCACAATG AGCGCAATGT GACCTGCACC CACCCAGTCA GCGGGCAACC CTCCCAGGAG 
AACTGTATCT TTGTGCTGAA TGAGCAGACT CCTCCCAGTG TTTCCTCTCA GGAACAGAAA 
GATCTCACAG TCAGTATGTC AAGTGAATCC CCCAATTGCA ACACTCAGCC CCCCGATAAT 
CCATCCAGCC CCCTGACTCG GTCATCCAGA TCTTCAGGCA AAATCCACAA TTTTGGCAAA 
AGGTCAAATT CTATAAAACG GAATCCAAAT GCTCCGGTGG CGATGCGCGG CTGGCTCTAC 
AAGAAGGCTA ATTCAGGCAT GAAGCTGTGG AAGAAGCGCT GGTTTGTGCT GTCGGACCTG 
TGTCTGTTCT ATTACAGAGA TGAGAAGGAA GAAGGGGTAC TGGGGAGCAT TCTCCTCCCG 
AGCTTTCAGA TACGCATGTT AACTCCCGAC GATCACATTA ACCGCTCGTT CGCCTTCAAG 
GCAGCCCACT CCAACATGAG GACCTATTAT TTCTGCACAG ACTCTGCCAA AGAAATGGAG 
CTGTGGATGA GAGCCATGAC TGATGCATCA CTTGTGCACT CAAAGCCTGC AAACAGGGTG 
GAACCGATCA AGTGTGAGAA AGTTGCCCCC CCAGAACTGA ACCATGTTGC AAATCACAGG 
CTGCTGATCA GGCCGGAAGC CAACAATAAC CAGAGAAACA CAGAGCTGAG CAGACTTGTG 
GGGAAGAAGT CCCTGGAGGC AGAGAAATAC GGGTTCCAGC AGGGTGAGAA GCCCTTAACC 
AAAATAACCA GTGGGAGGCC CGGCATGTCG GACTATGAGA CCCTGCACAC AGTCCAGTAC 
CGCTCAGCAC ACATTAATGG ACCTGACATC CAAGGCCATT TCTCTGAGCC GGACTCTGCT 
GACCCGCACA GAACTGTCAC AGCCAACACG GAACCCGAGC GGATCATGCA GAGAACTAAT 
TCCCTATTGC AGTTGGGACA GTGGATCCGA GCGCAGAGAG ATAAGGAGTT TGAGGGCGAG 
TCGAGAGGTG TAATGTCCTA TCAGACGTTG CCCAGAAATA TGCCCAGCCA CCACATGCAG 
ACACTGCCAC GTTACCCAGA AGGCTACCAG ACTCTCCCGC GGCAACCTAA AGCCCGGCCA 
GAGAGCCTGT GCAGCACCAC CGGATACGAG CAGAGCTTGG GGCCCATGAA TGTGACTGGT 
GAGGAGAAGA GGAGATCCAT TCGGGATGAT ACCATGTGGC AGTTGTACGA GTGGCAGCAG 
AGGCAGTTCT ACAACAAACA GACCACTATA CGCCGACACG GATCCCTCAC CAGCCCCAAA 
ACCATGATCC ACATATCCGA CCAGACGTTA CAGTCCATTC CCATGTCTCC TTCCCACGGC 
TCCATCTCTG GGTACCACAC GTATTCACCC CATCGCTCCT ACAGGTCAGA TATCTCCTCC 
CCCGTGCAGA TTGGGGACAT TACTATTGAC AGGAGGCACA AGGCTTTCTC TAATAAGCAT 
GCCTTTGTGC CCGACAGAAG GTCAATGCCC GCAGGAGTGA ACCTACAGCC CGTTACCCCA 
CAGGGTTTCC AAGGCAAGAC TATGGTGCAA GACGATGGTC GCTCCACGGC GCACAAATAC 
CGCTCCGAAG AACTGGACAT CGATGCCAAG CTGAGCCGCC TGTGTGAGCA GGAGAAAGTG 
GTTCAGGCCC TGGAGGAGAA GCTGCAACAG TTGCACAAGG AGAAGTACAC TCTGGAGCAG 
GCGCTGCTCT CTGCCAGCCA GGAGATAGAA CTGAATGCCG ACAATGCGAC CACCATACAG 
AGCGTGGTGC TACAGAGAGA CGACCTGCAG GACGGGCTGC TCAGCACCTG CCGGGAAGTG 
TCTCGCGCTA CTGCAGAGCT GGAGAGATCG TGGCGAGAAT ACGACAAGTT AGAGCGCGAT 
GTGACGGCAG CGAGGAACCA AATGCGGGAG CAGCTGGAGC GACTGGGAGG GGTGCAGACA 
GAAACGGGGG GGATTCAGCG CGCCCAGATG CAGAAGGAAA TCTGGAGAAT TCAGGATGTG 
ATGGAAGGAC TCAGCAAGCA CAAGCAGCAC AGAACCCAGG CTGCAGCCGA ATCCGAGTCA 
CACGAGATTT CTACGGGAAA GGGCGCAAAT GAGGGGCCAG ACTACAGGCT GTACAAGAGT 
GAGCCCGAGC TGACCACCGT AGCGGAAGTG GACGAGACCA ACGCTGATGA CAAGAGCGAG 
GCGGCTGTGA ATCTGGAAAG TGGCGCAAGT AAAGGGTCAC ACTTTCCCGT GGGGGTCGTG 
TCCCCAAGAG CCAAATCCCC TCCACTTGAA TCCACCACTA TTGCTTCTTA TGTCACTTTA 
AGGAAGGGCA AGAAGATGGA GCCAAAAACA GATCGGCCGA GGAGCGCGCT GGAACACCTG 
TGTATAGCGG ATACGCCCAG GACCCGCATG ACGGTGGAGG AGCAACTGGA GCGAATGAAG 
AAGCACCAGC AACCCTGCCT GCGAAAAAAG GCTTTCAGTT TATTTGGGGC CCCAGAGCAG 
TCGCCATCCC AGAGCCCTTC CTTCTCCAGG GAGAATTCTC TTAAAATGAT TCGCCGGCGG 
GAGGATAAAA TATTCAGTAA CATTAAAGAC ATAGAGAACG CGGTGCGAGA AAACTCCACG 
CAAAGGGAGT CTCCAGCCGA GGAGATTCTC AGACTGAAGG AAACAGCTGA GAGATCCGAC 
CCTGACTCAG AGGCCGGGGA CGCAGCCGGG GGTACAGGGA GTTTTGCTGT ATCTGATGCC 
AAAGAGACGC TGGAACTGGA CCCTCTTGTA CTTTCCAATC CTGTTGGGTC CGTTCAGTCA 
CCAGAAGAAA TCAGCAGCCC GGCCCCCCCA TCACTGACCA ATCACAAGCC GGATTTATGC 
TGCGCTACAG AGCCACTGGC ACCAGCCATT GTTTCCCCAT ATGACTTTGC TGCAGATCCT 
TTAAAGAGAC GCACATCATT GGAAGCCGCC ACCCCATCTC CCCCAGAGCC GTCACCAGCA 
CAAGCCCAGC TCACTGAAGG CACCCACTTC ATGTGTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018110198.1
CDS	16..3237
Translation	MIVVCICVTQVLVSPSEEAKSTTWLHPLTGETLLTGHRKTPDLPTGWQEGYTFEGARYYINHNERNVTCTHPVSGQPSQENCIFVLNEQTPPSVSSQEQKDLTVSMSSESPNCNTQPPDNPSSPLTRSSRSSGKIHNFGKRSNSIKRNPNAPVAMRGWLYKKANSGMKLWKKRWFVLSDLCLFYYRDEKEEGVLGSILLPSFQIRMLTPDDHINRSFAFKAAHSNMRTYYFCTDSAKEMELWMRAMTDASLVHSKPANRVEPIKCEKVAPPELNHVANHRLLIRPEANNNQRNTELSRLVGKKSLEAEKYGFQQGEKPLTKITSGRPGMSDYETLHTVQYRSAHINGPDIQGHFSEPDSADPHRTVTANTEPERIMQRTNSLLQLGQWIRAQRDKEFEGESRGVMSYQTLPRNMPSHHMQTLPRYPEGYQTLPRQPKARPESLCSTTGYEQSLGPMNVTGEEKRRSIRDDTMWQLYEWQQRQFYNKQTTIRRHGSLTSPKTMIHISDQTLQSIPMSPSHGSISGYHTYSPHRSYRSDISSPVQIGDITIDRRHKAFSNKHAFVPDRRSMPAGVNLQPVTPQGFQGKTMVQDDGRSTAHKYRSEELDIDAKLSRLCEQEKVVQALEEKLQQLHKEKYTLEQALLSASQEIELNADNATTIQSVVLQRDDLQDGLLSTCREVSRATAELERSWREYDKLERDVTAARNQMREQLERLGGVQTETGGIQRAQMQKEIWRIQDVMEGLSKHKQHRTQAAAESESHEISTGKGANEGPDYRLYKSEPELTTVAEVDETNADDKSEAAVNLESGASKGSHFPVGVVSPRAKSPPLESTTIASYVTLRKGKKMEPKTDRPRSALEHLCIADTPRTRMTVEEQLERMKKHQQPCLRKKAFSLFGAPEQSPSQSPSFSRENSLKMIRRREDKIFSNIKDIENAVRENSTQRESPAEEILRLKETAERSDPDSEAGDAAGGTGSFAVSDAKETLELDPLVLSNPVGSVQSPEEISSPAPPSLTNHKPDLCCATEPLAPAIVSPYDFAADPLKRRTSLEAATPSPPEPSPAQAQLTEGTHFMCV

Target ORF information:

RefSeq Version	XM_018254709.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis pleckstrin homology domain-containing family A member 5-like (LOC108712509), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018254709.1

ORF Insert Sequence:

ATGATTGTGG TGTGTATTTG TGTAACACAA GTCCTTGTCT CCCCCAGTGA GGAAGCAAAG 
AGTACCACCT GGCTGCACCC GCTCACTGGG GAGACTCTAC TCACAGGCCA CAGAAAGACC 
CCAGACTTGC CCACAGGGTG GCAGGAGGGC TACACGTTTG AAGGCGCTCG ATACTACATA 
AACCACAATG AGCGCAATGT GACCTGCACC CACCCAGTCA GCGGGCAACC CTCCCAGGAG 
AACTGTATCT TTGTGCTGAA TGAGCAGACT CCTCCCAGTG TTTCCTCTCA GGAACAGAAA 
GATCTCACAG TCAGTATGTC AAGTGAATCC CCCAATTGCA ACACTCAGCC CCCCGATAAT 
CCATCCAGCC CCCTGACTCG GTCATCCAGA TCTTCAGGCA AAATCCACAA TTTTGGCAAA 
AGGTCAAATT CTATAAAACG GAATCCAAAT GCTCCGGTGG CGATGCGCGG CTGGCTCTAC 
AAGAAGGCTA ATTCAGGCAT GAAGCTGTGG AAGAAGCGCT GGTTTGTGCT GTCGGACCTG 
TGTCTGTTCT ATTACAGAGA TGAGAAGGAA GAAGGGGTAC TGGGGAGCAT TCTCCTCCCG 
AGCTTTCAGA TACGCATGTT AACTCCCGAC GATCACATTA ACCGCTCGTT CGCCTTCAAG 
GCAGCCCACT CCAACATGAG GACCTATTAT TTCTGCACAG ACTCTGCCAA AGAAATGGAG 
CTGTGGATGA GAGCCATGAC TGATGCATCA CTTGTGCACT CAAAGCCTGC AAACAGGGTG 
GAACCGATCA AGTGTGAGAA AGTTGCCCCC CCAGAACTGA ACCATGTTGC AAATCACAGG 
CTGCTGATCA GGCCGGAAGC CAACAATAAC CAGAGAAACA CAGAGCTGAG CAGACTTGTG 
GGGAAGAAGT CCCTGGAGGC AGAGAAATAC GGGTTCCAGC AGGGTGAGAA GCCCTTAACC 
AAAATAACCA GTGGGAGGCC CGGCATGTCG GACTATGAGA CCCTGCACAC AGTCCAGTAC 
CGCTCAGCAC ACATTAATGG ACCTGACATC CAAGGCCATT TCTCTGAGCC GGACTCTGCT 
GACCCGCACA GAACTGTCAC AGCCAACACG GAACCCGAGC GGATCATGCA GAGAACTAAT 
TCCCTATTGC AGTTGGGACA GTGGATCCGA GCGCAGAGAG ATAAGGAGTT TGAGGGCGAG 
TCGAGAGGTG TAATGTCCTA TCAGACGTTG CCCAGAAATA TGCCCAGCCA CCACATGCAG 
ACACTGCCAC GTTACCCAGA AGGCTACCAG ACTCTCCCGC GGCAACCTAA AGCCCGGCCA 
GAGAGCCTGT GCAGCACCAC CGGATACGAG CAGAGCTTGG GGCCCATGAA TGTGACTGGT 
GAGGAGAAGA GGAGATCCAT TCGGGATGAT ACCATGTGGC AGTTGTACGA GTGGCAGCAG 
AGGCAGTTCT ACAACAAACA GACCACTATA CGCCGACACG GATCCCTCAC CAGCCCCAAA 
ACCATGATCC ACATATCCGA CCAGACGTTA CAGTCCATTC CCATGTCTCC TTCCCACGGC 
TCCATCTCTG GGTACCACAC GTATTCACCC CATCGCTCCT ACAGGTCAGA TATCTCCTCC 
CCCGTGCAGA TTGGGGACAT TACTATTGAC AGGAGGCACA AGGCTTTCTC TAATAAGCAT 
GCCTTTGTGC CCGACAGAAG GTCAATGCCC GCAGGAGTGA ACCTACAGCC CGTTACCCCA 
CAGGGTTTCC AAGGCAAGAC TATGGTGCAA GACGATGGTC GCTCCACGGC GCACAAATAC 
CGCTCCGAAG AACTGGACAT CGATGCCAAG CTGAGCCGCC TGTGTGAGCA GGAGAAAGTG 
GTTCAGGCCC TGGAGGAGAA GCTGCAACAG TTGCACAAGG AGAAGTACAC TCTGGAGCAG 
GCGCTGCTCT CTGCCAGCCA GGAGATAGAA CTGAATGCCG ACAATGCGAC CACCATACAG 
AGCGTGGTGC TACAGAGAGA CGACCTGCAG GACGGGCTGC TCAGCACCTG CCGGGAAGTG 
TCTCGCGCTA CTGCAGAGCT GGAGAGATCG TGGCGAGAAT ACGACAAGTT AGAGCGCGAT 
GTGACGGCAG CGAGGAACCA AATGCGGGAG CAGCTGGAGC GACTGGGAGG GGTGCAGACA 
GAAACGGGGG GGATTCAGCG CGCCCAGATG CAGAAGGAAA TCTGGAGAAT TCAGGATGTG 
ATGGAAGGAC TCAGCAAGCA CAAGCAGCAC AGAACCCAGG CTGCAGCCGA ATCCGAGTCA 
CACGAGATTT CTACGGGAAA GGGCGCAAAT GAGGGGCCAG ACTACAGGCT GTACAAGAGT 
GAGCCCGAGC TGACCACCGT AGCGGAAGTG GACGAGACCA ACGCTGATGA CAAGAGCGAG 
GCGGCTGTGA ATCTGGAAAG TGGCGCAAGT AAAGGGTCAC ACTTTCCCGT GGGGGTCGTG 
TCCCCAAGAG CCAAATCCCC TCCACTTGAA TCCACCACTA TTGCTTCTTA TGTCACTTTA 
AGGAAGGGCA AGAAGATGGA GCCAAAAACA GATCGGCCGA GGAGCGCGCT GGAACACCTG 
TGTATAGCGG ATACGCCCAG GACCCGCATG ACGGTGGAGG AGCAACTGGA GCGAATGAAG 
AAGCACCAGC AACCCTGCCT GCGAAAAAAG GCTTTCAGTT TATTTGGGGC CCCAGAGCAG 
TCGCCATCCC AGAGCCCTTC CTTCTCCAGG GAGAATTCTC TTAAAATGAT TCGCCGGCGG 
GAGGATAAAA TATTCAGTAA CATTAAAGAC ATAGAGAACG CGGTGCGAGA AAACTCCACG 
CAAAGGGAGT CTCCAGCCGA GGAGATTCTC AGACTGAAGG AAACAGCTGA GAGATCCGAC 
CCTGACTCAG AGGCCGGGGA CGCAGCCGGG GGTACAGGGA GTTTTGCTGT ATCTGATGCC 
AAAGAGACGC TGGAACTGGA CCCTCTTGTA CTTTCCAATC CTGTTGGGTC CGTTCAGTCA 
CCAGAAGAAA TCAGCAGCCC GGCCCCCCCA TCACTGACCA ATCACAAGCC GGATTTATGC 
TGCGCTACAG AGCCACTGGC ACCAGCCATT GTTTCCCCAT ATGACTTTGC TGCAGATCCT 
TTAAAGAGAC GCACATCATT GGAAGCCGCC ACCCCATCTC CCCCAGAGCC GTCACCAGCA 
CAAGCCCAGC TCACTGAAGG CACCCACTTC ATGTGTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108712509 ( XM_018254710.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018110199.1 Xenopus laevis pleckstrin homology domain-containing family A member 5-like (LOC108712509), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf26232
Clone ID Related Accession (Same CDS sequence)	XM_018254710.1
Accession Version	XM_018254710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	pleckstrin homology domain-containing family A member 5-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030729.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGTGTATTTG TGTAACACAA GTCCTTGTCT CCCCCAGTGA GGAAGCAAAG 
AGTACCACCT GGCTGCACCC GCTCACTGGG GAGACTCTAC TCACAGGCCA CAGAAAGACC 
CCAGACTTGC CCACAGGGTG GCAGGAGGGC TACACGTTTG AAGGCGCTCG ATACTACATA 
AACCACAATG AGCGCAATGT GACCTGCACC CACCCAGTCA GCGGGCAACC CTCCCAGGAG 
AACTGTATCT TTGTGCTGAA TGAGCAGACT CCTCCCAGTG TTTCCTCTCA GGAACAGAAA 
GATCTCACAG TCAGTATGTC AAGTGAATCC CCCAATTGCA ACACTCAGCC CCCCGATAAT 
CCATCCAGCC CCCTGACTCG GTCATCCAGA TCTTCAGGCA AAATCCACAA TTTTGGCAAA 
AGGTCAAATT CTATAAAACG GAATCCAAAT GCTCCGGTGG CGATGCGCGG CTGGCTCTAC 
AAGAAGGCTA ATTCAGGCAT GAAGCTGTGG AAGAAGCGCT GGTTTGTGCT GTCGGACCTG 
TGTCTGTTCT ATTACAGAGA TGAGAAGGAA GAAGGGGTAC TGGGGAGCAT TCTCCTCCCG 
AGCTTTCAGA TACGCATGTT AACTCCCGAC GATCACATTA ACCGCTCGTT CGCCTTCAAG 
GCAGCCCACT CCAACATGAG GACCTATTAT TTCTGCACAG ACTCTGCCAA AGAAATGGAG 
CTGTGGATGA GAGCCATGAC TGATGCATCA CTTGTGCACT CAAAGCCTGC AAACAGGGTG 
GAACCGATCA AGTGTGAGAA AGTTGCCCCC CCAGAACTGA ACCATGTTGC AAATCACAGG 
CTGCTGATCA GGCCGGAAGC CAACAATAAC CAGAGAAACA CAGAGCTGAG CAGACTTGTG 
GGGAAGAAGT CCCTGGAGGC AGAGAAATAC GGGTTCCAGC AGGGTGAGAA GCCCTTAACC 
AAAATAACCA GTGGGAGGCC CGGCATGTCG GACTATGAGA CCCTGCACAC AGTCCAGTAC 
CGCTCAGCAC ACATTAATGG ACCTGACATC CAAGGCCATT TCTCTGAGCC GGACTCTGCT 
GACCCGCACA GAACTGTCAC AGCCAACACG GAACCCGAGC GGATCATGCA GAGAACTAAT 
TCCCTATTGC AGTTGGGACA GTGGATCCGA GCGCAGAGAG ATAAGGAGTT TGAGGGCGAG 
TCGAGAGGTG TAATGTCCTA TCAGACGTTG CCCAGAAATA TGCCCAGCCA CCACATGCAG 
ACACTGCCAC GTTACCCAGA AGGCTACCAG ACTCTCCCGC GGCAACCTAA AGCCCGGCCA 
GAGAGCCTGT GCAGCACCAC CGGATACGAG CAGAGCTTGG GGCCCATGAA TGTGACTGGT 
GAGGAGAAGA GGAGATCCAT TCGGGATGAT ACCATGTGGC AGTTGTACGA GTGGCAGCAG 
AGGCAGTTCT ACAACAAACA GACCACTATA CGCCGACACG GATCCCTCAC CAGCCCCAAA 
ACCATGATCC ACATATCCGA CCAGACGTTA CAGTCCATTC CCATGTCTCC TTCCCACGGC 
TCCATCTCTG GGTACCACAC GTATTCACCC CATCGCTCCT ACAGGTCAGA TATCTCCTCC 
CCCGTGCAGA TTGGGGACAT TACTATTGAC AGGAGGCACA AGGCTTTCTC TAATAAGCAT 
GCCTTTGTGC CCGACAGAAG GTCAATGCCC GCAGGAGTGA ACCTACAGCC CGTTACCCCA 
CAGGGTTTCC AAGGCAAGAC TATGGTGCAA GACGATGGTC GCTCCACGGC GCACAAATAC 
CGCTCCGAAG AACTGGACAT CGATGCCAAG CTGAGCCGCC TGTGTGAGCA GGAGAAAGTG 
GTTCAGGCCC TGGAGGAGAA GCTGCAACAG TTGCACAAGG AGAAGTACAC TCTGGAGCAG 
GCGCTGCTCT CTGCCAGCCA GGAGATAGAA CTGAATGCCG ACAATGCGAC CACCATACAG 
AGCGTGGTGC TACAGAGAGA CGACCTGCAG GACGGGCTGC TCAGCACCTG CCGGGAAGTG 
TCTCGCGCTA CTGCAGAGCT GGAGAGATCG TGGCGAGAAT ACGACAAGTT AGAGCGCGAT 
GTGACGGCAG CGAGGAACCA AATGCGGGAG CAGCTGGAGC GACTGGGAGG GGTGCAGACA 
GAAACGGGGG GGATTCAGCG CGCCCAGATG CAGAAGGAAA TCTGGAGAAT TCAGGATGTG 
ATGGAAGGAC TCAGCAAGCA CAAGCAGCAC AGAACCCAGG CTGCAGCCGA ATCCGAGTCA 
CACGAGATTT CTACGGGAAA GGGCGCAAAT GAGGGGCCAG ACTACAGGCT GTACAAGAGT 
GAGCCCGAGC TGACCACCGT AGCGGAAGTG GACGAGACCA ACGCTGATGA CAAGAGCGAG 
GCGGCTGTGA ATCTGGAAAG TGGCGCAAGT AAAGGGTCAC ACTTTCCCGT GGGGGTCGTG 
TCCCCAAGAG CCAAATCCCC TCCACTTGAA TCCACCACTA TTGCTTCTTA TGTCACTTTA 
AGGAAGGGCA AGAAGATGGA GCCAAAAACA GATCGGCCGA GGAGCGCGCT GGAACACCTG 
TGTATAGCGG ATACGCCCAG GACCCGCATG ACGGTGGAGG AGCAACTGGA GCGAATGAAG 
AAGCACCAGC AACCCTGCCT GCGAAAAAAG GCTTTCAGTT TATTTGGGGC CCCAGAGCAG 
TCGCCATCCC AGAGCCCTTC CTTCTCCAGG GAGAATTCTC TTAAAATGAT TCGCCGGCGG 
GAGGATAAAA TATTCAGTAA CATTAAAGAC ATAGAGAACG CGGTGCGAGA AAACTCCACG 
CAAAGGGAGT CTCCAGCCGA GGAGATTCTC AGACTGAAGG AAACAGCTGA GAGATCCGAC 
CCTGACTCAG AGGCCGGGGA CGCAGCCGGG GGTACAGGGA GTTTTGCTGT ATCTGATGCC 
AAAGAGACGC TGGAACTGGA CCCTCTTGTA CTTTCCAATC CTGTTGGGTC CGTTCAGTCA 
CCAGAAGAAA TCAGCCCGGC CCCCCCATCA CTGACCAATC ACAAGCCGGA TTTATGCTGC 
GCTACAGAGC CACTGGCACC AGCCATTGTT TCCCCATATG ACTTTGCTGC AGATCCTTTA 
AAGAGACGCA CATCATTGGA AGCCGCCACC CCATCTCCCC CAGAGCCGTC ACCAGCACAA 
GCCCAGCTCA CTGAAGGCAC CCACTTCATG TGTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018110199.1
CDS	16..3234
Translation	MIVVCICVTQVLVSPSEEAKSTTWLHPLTGETLLTGHRKTPDLPTGWQEGYTFEGARYYINHNERNVTCTHPVSGQPSQENCIFVLNEQTPPSVSSQEQKDLTVSMSSESPNCNTQPPDNPSSPLTRSSRSSGKIHNFGKRSNSIKRNPNAPVAMRGWLYKKANSGMKLWKKRWFVLSDLCLFYYRDEKEEGVLGSILLPSFQIRMLTPDDHINRSFAFKAAHSNMRTYYFCTDSAKEMELWMRAMTDASLVHSKPANRVEPIKCEKVAPPELNHVANHRLLIRPEANNNQRNTELSRLVGKKSLEAEKYGFQQGEKPLTKITSGRPGMSDYETLHTVQYRSAHINGPDIQGHFSEPDSADPHRTVTANTEPERIMQRTNSLLQLGQWIRAQRDKEFEGESRGVMSYQTLPRNMPSHHMQTLPRYPEGYQTLPRQPKARPESLCSTTGYEQSLGPMNVTGEEKRRSIRDDTMWQLYEWQQRQFYNKQTTIRRHGSLTSPKTMIHISDQTLQSIPMSPSHGSISGYHTYSPHRSYRSDISSPVQIGDITIDRRHKAFSNKHAFVPDRRSMPAGVNLQPVTPQGFQGKTMVQDDGRSTAHKYRSEELDIDAKLSRLCEQEKVVQALEEKLQQLHKEKYTLEQALLSASQEIELNADNATTIQSVVLQRDDLQDGLLSTCREVSRATAELERSWREYDKLERDVTAARNQMREQLERLGGVQTETGGIQRAQMQKEIWRIQDVMEGLSKHKQHRTQAAAESESHEISTGKGANEGPDYRLYKSEPELTTVAEVDETNADDKSEAAVNLESGASKGSHFPVGVVSPRAKSPPLESTTIASYVTLRKGKKMEPKTDRPRSALEHLCIADTPRTRMTVEEQLERMKKHQQPCLRKKAFSLFGAPEQSPSQSPSFSRENSLKMIRRREDKIFSNIKDIENAVRENSTQRESPAEEILRLKETAERSDPDSEAGDAAGGTGSFAVSDAKETLELDPLVLSNPVGSVQSPEEISPAPPSLTNHKPDLCCATEPLAPAIVSPYDFAADPLKRRTSLEAATPSPPEPSPAQAQLTEGTHFMCV

Target ORF information:

RefSeq Version	XM_018254710.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis pleckstrin homology domain-containing family A member 5-like (LOC108712509), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018254710.1

ORF Insert Sequence:

ATGATTGTGG TGTGTATTTG TGTAACACAA GTCCTTGTCT CCCCCAGTGA GGAAGCAAAG 
AGTACCACCT GGCTGCACCC GCTCACTGGG GAGACTCTAC TCACAGGCCA CAGAAAGACC 
CCAGACTTGC CCACAGGGTG GCAGGAGGGC TACACGTTTG AAGGCGCTCG ATACTACATA 
AACCACAATG AGCGCAATGT GACCTGCACC CACCCAGTCA GCGGGCAACC CTCCCAGGAG 
AACTGTATCT TTGTGCTGAA TGAGCAGACT CCTCCCAGTG TTTCCTCTCA GGAACAGAAA 
GATCTCACAG TCAGTATGTC AAGTGAATCC CCCAATTGCA ACACTCAGCC CCCCGATAAT 
CCATCCAGCC CCCTGACTCG GTCATCCAGA TCTTCAGGCA AAATCCACAA TTTTGGCAAA 
AGGTCAAATT CTATAAAACG GAATCCAAAT GCTCCGGTGG CGATGCGCGG CTGGCTCTAC 
AAGAAGGCTA ATTCAGGCAT GAAGCTGTGG AAGAAGCGCT GGTTTGTGCT GTCGGACCTG 
TGTCTGTTCT ATTACAGAGA TGAGAAGGAA GAAGGGGTAC TGGGGAGCAT TCTCCTCCCG 
AGCTTTCAGA TACGCATGTT AACTCCCGAC GATCACATTA ACCGCTCGTT CGCCTTCAAG 
GCAGCCCACT CCAACATGAG GACCTATTAT TTCTGCACAG ACTCTGCCAA AGAAATGGAG 
CTGTGGATGA GAGCCATGAC TGATGCATCA CTTGTGCACT CAAAGCCTGC AAACAGGGTG 
GAACCGATCA AGTGTGAGAA AGTTGCCCCC CCAGAACTGA ACCATGTTGC AAATCACAGG 
CTGCTGATCA GGCCGGAAGC CAACAATAAC CAGAGAAACA CAGAGCTGAG CAGACTTGTG 
GGGAAGAAGT CCCTGGAGGC AGAGAAATAC GGGTTCCAGC AGGGTGAGAA GCCCTTAACC 
AAAATAACCA GTGGGAGGCC CGGCATGTCG GACTATGAGA CCCTGCACAC AGTCCAGTAC 
CGCTCAGCAC ACATTAATGG ACCTGACATC CAAGGCCATT TCTCTGAGCC GGACTCTGCT 
GACCCGCACA GAACTGTCAC AGCCAACACG GAACCCGAGC GGATCATGCA GAGAACTAAT 
TCCCTATTGC AGTTGGGACA GTGGATCCGA GCGCAGAGAG ATAAGGAGTT TGAGGGCGAG 
TCGAGAGGTG TAATGTCCTA TCAGACGTTG CCCAGAAATA TGCCCAGCCA CCACATGCAG 
ACACTGCCAC GTTACCCAGA AGGCTACCAG ACTCTCCCGC GGCAACCTAA AGCCCGGCCA 
GAGAGCCTGT GCAGCACCAC CGGATACGAG CAGAGCTTGG GGCCCATGAA TGTGACTGGT 
GAGGAGAAGA GGAGATCCAT TCGGGATGAT ACCATGTGGC AGTTGTACGA GTGGCAGCAG 
AGGCAGTTCT ACAACAAACA GACCACTATA CGCCGACACG GATCCCTCAC CAGCCCCAAA 
ACCATGATCC ACATATCCGA CCAGACGTTA CAGTCCATTC CCATGTCTCC TTCCCACGGC 
TCCATCTCTG GGTACCACAC GTATTCACCC CATCGCTCCT ACAGGTCAGA TATCTCCTCC 
CCCGTGCAGA TTGGGGACAT TACTATTGAC AGGAGGCACA AGGCTTTCTC TAATAAGCAT 
GCCTTTGTGC CCGACAGAAG GTCAATGCCC GCAGGAGTGA ACCTACAGCC CGTTACCCCA 
CAGGGTTTCC AAGGCAAGAC TATGGTGCAA GACGATGGTC GCTCCACGGC GCACAAATAC 
CGCTCCGAAG AACTGGACAT CGATGCCAAG CTGAGCCGCC TGTGTGAGCA GGAGAAAGTG 
GTTCAGGCCC TGGAGGAGAA GCTGCAACAG TTGCACAAGG AGAAGTACAC TCTGGAGCAG 
GCGCTGCTCT CTGCCAGCCA GGAGATAGAA CTGAATGCCG ACAATGCGAC CACCATACAG 
AGCGTGGTGC TACAGAGAGA CGACCTGCAG GACGGGCTGC TCAGCACCTG CCGGGAAGTG 
TCTCGCGCTA CTGCAGAGCT GGAGAGATCG TGGCGAGAAT ACGACAAGTT AGAGCGCGAT 
GTGACGGCAG CGAGGAACCA AATGCGGGAG CAGCTGGAGC GACTGGGAGG GGTGCAGACA 
GAAACGGGGG GGATTCAGCG CGCCCAGATG CAGAAGGAAA TCTGGAGAAT TCAGGATGTG 
ATGGAAGGAC TCAGCAAGCA CAAGCAGCAC AGAACCCAGG CTGCAGCCGA ATCCGAGTCA 
CACGAGATTT CTACGGGAAA GGGCGCAAAT GAGGGGCCAG ACTACAGGCT GTACAAGAGT 
GAGCCCGAGC TGACCACCGT AGCGGAAGTG GACGAGACCA ACGCTGATGA CAAGAGCGAG 
GCGGCTGTGA ATCTGGAAAG TGGCGCAAGT AAAGGGTCAC ACTTTCCCGT GGGGGTCGTG 
TCCCCAAGAG CCAAATCCCC TCCACTTGAA TCCACCACTA TTGCTTCTTA TGTCACTTTA 
AGGAAGGGCA AGAAGATGGA GCCAAAAACA GATCGGCCGA GGAGCGCGCT GGAACACCTG 
TGTATAGCGG ATACGCCCAG GACCCGCATG ACGGTGGAGG AGCAACTGGA GCGAATGAAG 
AAGCACCAGC AACCCTGCCT GCGAAAAAAG GCTTTCAGTT TATTTGGGGC CCCAGAGCAG 
TCGCCATCCC AGAGCCCTTC CTTCTCCAGG GAGAATTCTC TTAAAATGAT TCGCCGGCGG 
GAGGATAAAA TATTCAGTAA CATTAAAGAC ATAGAGAACG CGGTGCGAGA AAACTCCACG 
CAAAGGGAGT CTCCAGCCGA GGAGATTCTC AGACTGAAGG AAACAGCTGA GAGATCCGAC 
CCTGACTCAG AGGCCGGGGA CGCAGCCGGG GGTACAGGGA GTTTTGCTGT ATCTGATGCC 
AAAGAGACGC TGGAACTGGA CCCTCTTGTA CTTTCCAATC CTGTTGGGTC CGTTCAGTCA 
CCAGAAGAAA TCAGCCCGGC CCCCCCATCA CTGACCAATC ACAAGCCGGA TTTATGCTGC 
GCTACAGAGC CACTGGCACC AGCCATTGTT TCCCCATATG ACTTTGCTGC AGATCCTTTA 
AAGAGACGCA CATCATTGGA AGCCGCCACC CCATCTCCCC CAGAGCCGTC ACCAGCACAA 
GCCCAGCTCA CTGAAGGCAC CCACTTCATG TGTGTATAG