Actg2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actg2
Entrez Gene ID	25365
Full Name	actin, gamma 2, smooth muscle, enteric
Synonyms	ACTGE,SMGA
General protein information	Preferred Names actin, gamma 2, smooth muscle, enteric Names actin, gamma-enteric smooth muscle alpha-actin-3 alpha-smooth muscle actin gamma-2-actin gamma-enteric smooth muscle actin smooth muscle gamma-actin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	may play a role in smooth muscle function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012893.1	NP_037025.1	actin, gamma-enteric smooth muscle
XM_006236728.2	XP_006236790.1	actin, gamma-enteric smooth muscle isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTG2	XP_533002.2
Bos taurus (cattle)	ACTG2	NP_001013610.1
Gallus gallus (chicken)	ACTG2	NP_990503.1
Arabidopsis thaliana (thale cress)	ACT12	NP_190236.1
	ACTG2	XP_001105970.1
Mus musculus (house mouse)	Actg2	NP_033740.2
Danio rerio (zebrafish)	acta2	NP_997785.1
Arabidopsis thaliana (thale cress)	ACT4	NP_001078769.1
Homo sapiens (human)	ACTG2	NP_001606.1
Pan troglodytes (chimpanzee)	ACTG2	XP_001152416.2
Rattus norvegicus (Norway rat)	Actg2	NP_037025.1
Drosophila melanogaster (fruit fly)	Act79B	NP_001262200.1
Oryza sativa (rice)	Os10g0510000	NP_001065034.1
Xenopus tropicalis (tropical clawed frog)	acta2	NP_001011250.1

Gene Ontology
Bibliography

Related articles in PubMed

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A, Del Boccio P
Molecular bioSystems9(6)1108-16(2013 Jun)

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics75(12)3574-84(2012 Jun)

Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of alpha-smooth muscle actin and transforming growth factor-beta1.
Wang HY, Wang YJ, Cui MJ, Gu CM, Yang LZ, Zhao Y, Chen Y, Zhao D, Li TS, Chi BR
Indian journal of biochemistry & biophysics48(5)308-15(2011 Oct)

Myocardin-dependent activation of the CArG box-rich smooth muscle gamma-actin gene: preferential utilization of a single CArG element through functional association with the NKX3.1 homeodomain protein.
Sun Q, Taurin S, Sethakorn N, Long X, Imamura M, Wang DZ, Zimmer WE, Dulin NO, Miano JM
The Journal of biological chemistry284(47)32582-90(2009 Nov)

REM sleep deprivation attenuates actin-binding protein cortactin: a link between sleep and hippocampal plasticity.
Davis CJ, Meighan PC, Taishi P, Krueger JM, Harding JW, Wright JW
Neuroscience letters400(3)191-6(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of alpha-smooth muscle actin and transforming growth factor-beta1
Title: Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of alpha-smooth muscle actin and transforming growth factor-beta1.

MYOCD can discriminate among several juxtaposed CArG elements, presumably through its novel partnership with NKX3.1, to optimally transactivate the human ACTG2 promoter
Title: Myocardin-dependent activation of the CArG box-rich smooth muscle gamma-actin gene: preferential utilization of a single CArG element through functional association with the NKX3.1 homeodomain protein.

demonstrate the alterations of alpha-smooth muscle actin and vimentin localization patterns in the bovine cryptorchid testis as compared to the contralateral scrotal testis during postnatal development.
Title: Alterations in the immunohistochemical localization patterns of alpha-smooth muscle actin (SMA) and vimentin in the postnatally developing bovine cryptorchid testis.

Thus, these data support a functional link between REMS and cytoskeletal reorganization in the hippocampus, a process that is essential for synaptic plasticity.
Title: REM sleep deprivation attenuates actin-binding protein cortactin: a link between sleep and hippocampal plasticity.

PPAR gamma expression increases in rat renal tissue after unilateral ureteral obstruction but does not always correlate with alpha-smooth muscle actin expression.
Title: [Expression of peroxisome proliferators-activated receptor gamma and alpha-smooth muscle actin in unilateral ureteral obstruction in rats].

The following Actg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa27376	XM_006236728.2 Latest version!	Rattus norvegicus actin, gamma 2, smooth muscle, enteric (Actg2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa01232	NM_012893.1 Latest version!	Rattus norvegicus actin gamma 2, smooth muscle (Actg2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actg2 ( XM_006236728.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236790.1 Rattus norvegicus actin, gamma 2, smooth muscle, enteric (Actg2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27376
Clone ID Related Accession (Same CDS sequence)	XM_006236728.2
Accession Version	XM_006236728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin, gamma-enteric smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006236728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGAAG AAGAGACCAC CGCCCTTGTG TGTGACAATG GGTCTGGCCT GTGCAAGGCA 
GGCTTTGCAG GAGACGACGC TCCCAGGGCT GTCTTTCCCT CCATTGTGGG CCGCCCTCGG 
CATCAGGGCG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA CGAAGCCCAG 
AGCAAGCGTG GGATCCTGAC CCTCAAATAC CCCATTGAAC ACGGCATCAT CACGAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAACG AGCTGCGAGT AGCACCAGAA 
GAGCACCCGA CCCTGCTCAC AGAGGCCCCC CTAAACCCTA AAGCCAACAG GGAGAAGATG 
ACCCAGATCA TGTTCGAAAC CTTCAATGTT CCTGCCATGT ATGTTGCCAT TCAAGCTGTG 
CTCTCGCTCT ATGCATCTGG CCGCACCACA GGCATCGTCC TGGATTCAGG GGATGGCGTC 
ACCCACAATG TCCCCATCTA CGAGGGCTAT GCACTGCCCC ATGCCATCAT GCGTCTTGAC 
CTGGCTGGAC GGGATCTCAC AGACTACCTC ATGAAGATTC TCACAGAAAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTACGTA 
GCCCTGGATT TTGAGAATGA GATGGCCACG GCGGCTTCGT CTTCTTCCCT GGAGAAGAGC 
TACGAGTTGC CTGATGGGCA GGTCATCACG ATTGGCAATG AGCGCTTCCG CTGCCCGGAG 
ACCCTGTTCC AGCCTTCCTT CATTGGCATG GAGTCAGCTG GAATTCATGA GACAACATAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGCAAGGATT TGTATGCTAA CAATGTCCTC 
TCTGGGGGCA CTACCATGTA CCCTGGGATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
TTGGCTCCCA GCACCATGAA GATCAAGATC ATCGCTCCTC CGGAGCGGAA GTACTCAGTC 
TGGATTGGAG GCTCCATCCT GGCTTCTCTC TCCACCTTCC AGCAAATGTG GATCAGCAAG 
CCAGAGTATG ATGAAGCGGG GCCCTCCATT GTCCACAGGA AATGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236790.1
CDS	137..1267
Translation	MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	XM_006236728.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin, gamma 2, smooth muscle, enteric (Actg2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236728.2

ORF Insert Sequence:

ATGTGTGAAG AAGAGACCAC CGCCCTTGTG TGTGACAATG GGTCTGGCCT GTGCAAGGCA 
GGCTTTGCAG GAGACGACGC TCCCAGGGCT GTCTTTCCCT CCATTGTGGG CCGCCCTCGG 
CATCAGGGCG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA CGAAGCCCAG 
AGCAAGCGTG GGATCCTGAC CCTCAAATAC CCCATTGAAC ACGGCATCAT CACGAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAACG AGCTGCGAGT AGCACCAGAA 
GAGCACCCGA CCCTGCTCAC AGAGGCCCCC CTAAACCCTA AAGCCAACAG GGAGAAGATG 
ACCCAGATCA TGTTCGAAAC CTTCAATGTT CCTGCCATGT ATGTTGCCAT TCAAGCTGTG 
CTCTCGCTCT ATGCATCTGG CCGCACCACA GGCATCGTCC TGGATTCAGG GGATGGCGTC 
ACCCACAATG TCCCCATCTA CGAGGGCTAT GCACTGCCCC ATGCCATCAT GCGTCTTGAC 
CTGGCTGGAC GGGATCTCAC AGACTACCTC ATGAAGATTC TCACAGAAAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTACGTA 
GCCCTGGATT TTGAGAATGA GATGGCCACG GCGGCTTCGT CTTCTTCCCT GGAGAAGAGC 
TACGAGTTGC CTGATGGGCA GGTCATCACG ATTGGCAATG AGCGCTTCCG CTGCCCGGAG 
ACCCTGTTCC AGCCTTCCTT CATTGGCATG GAGTCAGCTG GAATTCATGA GACAACATAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGCAAGGATT TGTATGCTAA CAATGTCCTC 
TCTGGGGGCA CTACCATGTA CCCTGGGATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
TTGGCTCCCA GCACCATGAA GATCAAGATC ATCGCTCCTC CGGAGCGGAA GTACTCAGTC 
TGGATTGGAG GCTCCATCCT GGCTTCTCTC TCCACCTTCC AGCAAATGTG GATCAGCAAG 
CCAGAGTATG ATGAAGCGGG GCCCTCCATT GTCCACAGGA AATGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actg2 ( NM_012893.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037025.1 Rattus norvegicus actin gamma 2, smooth muscle (Actg2), mRNA.

Price & Availability↑

CloneID	ORa01232
Clone ID Related Accession (Same CDS sequence)	NM_012893.1
Accession Version	NM_012893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	actin, gamma-enteric smooth muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M22323.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M22323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689595, SAMEA2689596 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGAAG AAGAGACCAC CGCCCTTGTG TGTGACAATG GGTCTGGCCT GTGCAAGGCA 
GGCTTTGCAG GAGACGACGC TCCCAGGGCT GTCTTTCCCT CCATTGTGGG CCGCCCTCGG 
CATCAGGGCG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA CGAAGCCCAG 
AGCAAGCGTG GGATCCTGAC CCTCAAATAC CCCATTGAGC ACGGCATCAT CACGAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAACG AGCTGCGAGT AGCACCAGAA 
GAGCACCCGA CCCTGCTCAC AGAGGCCCCC CTAAACCCTA AAGCCAACAG GGAGAAGATG 
ACCCAGATCA TGTTCGAAAC CTTCAATGTT CCTGCCATGT ATGTTGCCAT TCAAGCTGTG 
CTCTCGCTCT ATGCATCTGG CCGCACCACA GGCATCGTCC TGGATTCAGG GGATGGCGTC 
ACCCACAATG TCCCCATCTA CGAGGGCTAT GCACTGCCCC ATGCCATCAT GCGTCTTGAC 
CTGGCTGGAC GGGATCTCAC AGACTACCTC ATGAAAATTC TCACAGAAAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTACGTA 
GCCCTGGATT TTGAGAATGA GATGGCCACG GCGGCTTCGT CTTCTTCCCT GGAGAAGAGC 
TACGAGTTGC CTGATGGGCA GGTCATCACG ATTGGCAATG AGCGCTTCCG CTGCCCGGAG 
ACCCTGTTCC AGCCTTCCTT CATTGGCATG GAGTCAGCTG GAATTCATGA GACAACATAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGCAAGGATT TGTATGCTAA CAATGTCCTC 
TCTGGGGGCA CTACCATGTA CCCTGGGATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
TTGGCTCCCA GCACCATGAA GATCAAGATC ATCGCTCCTC CGGAGCGGAA GTACTCAGTC 
TGGATTGGAG GCTCCATCCT GGCTTCTCTC TCCACCTTCC AGCAAATGTG GATCAGCAAG 
CCAGAGTATG ATGAAGCGGG GCCCTCCATT GTCCACAGGA AATGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037025.1
CDS	16..1146
Translation	MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_012893.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin gamma 2, smooth muscle (Actg2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012893.1

ORF Insert Sequence:

ATGTGTGAAG AAGAGACCAC CGCCCTTGTG TGTGACAATG GGTCTGGCCT GTGCAAGGCA 
GGCTTTGCAG GAGACGACGC TCCCAGGGCT GTCTTTCCCT CCATTGTGGG CCGCCCTCGG 
CATCAGGGCG TGATGGTGGG AATGGGCCAG AAAGACAGCT ATGTGGGGGA CGAAGCCCAG 
AGCAAGCGTG GGATCCTGAC CCTCAAATAC CCCATTGAGC ACGGCATCAT CACGAACTGG 
GATGACATGG AGAAGATCTG GCACCACTCC TTCTACAACG AGCTGCGAGT AGCACCAGAA 
GAGCACCCGA CCCTGCTCAC AGAGGCCCCC CTAAACCCTA AAGCCAACAG GGAGAAGATG 
ACCCAGATCA TGTTCGAAAC CTTCAATGTT CCTGCCATGT ATGTTGCCAT TCAAGCTGTG 
CTCTCGCTCT ATGCATCTGG CCGCACCACA GGCATCGTCC TGGATTCAGG GGATGGCGTC 
ACCCACAATG TCCCCATCTA CGAGGGCTAT GCACTGCCCC ATGCCATCAT GCGTCTTGAC 
CTGGCTGGAC GGGATCTCAC AGACTACCTC ATGAAAATTC TCACAGAAAG AGGCTATTCC 
TTTGTGACCA CAGCTGAGAG AGAAATTGTG CGAGACATCA AGGAGAAGCT GTGCTACGTA 
GCCCTGGATT TTGAGAATGA GATGGCCACG GCGGCTTCGT CTTCTTCCCT GGAGAAGAGC 
TACGAGTTGC CTGATGGGCA GGTCATCACG ATTGGCAATG AGCGCTTCCG CTGCCCGGAG 
ACCCTGTTCC AGCCTTCCTT CATTGGCATG GAGTCAGCTG GAATTCATGA GACAACATAC 
AATTCCATCA TGAAGTGTGA CATTGACATC CGCAAGGATT TGTATGCTAA CAATGTCCTC 
TCTGGGGGCA CTACCATGTA CCCTGGGATT GCTGACAGGA TGCAGAAGGA GATCACAGCC 
TTGGCTCCCA GCACCATGAA GATCAAGATC ATCGCTCCTC CGGAGCGGAA GTACTCAGTC 
TGGATTGGAG GCTCCATCCT GGCTTCTCTC TCCACCTTCC AGCAAATGTG GATCAGCAAG 
CCAGAGTATG ATGAAGCGGG GCCCTCCATT GTCCACAGGA AATGCTTCTA A